• Title/Summary/Keyword: Flavobacterlum multivorum

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Isolation of Flavobacterium multivorum Producing Exo-dextranase (세포외 덱스트란 분해효소를 생산하는 Flavobacterium multivorum의 분리)

  • 정재호;이형환;김영희;이희무
    • Korean Journal of Microbiology
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    • v.25 no.4
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    • pp.346-352
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    • 1987
  • One hundred and seventeen colonies were screened for the detection of the production of exodextranase on the dextran-mineral salts medium. Ten colonies out of them produced the dextranase. Flavobacterium multivorum greatly producing the enzyme was isolated from soil, identified and then studied for various biochemical characteristics. The activity of the dextranase in the cultured medium was high between pH8 and 9 at $35^{\circ}C$, and between $45^{\circ}C$ and $55^{\circ}C$ at pH8. By the growth curves the generation times of the bacterium were approximately 52 minutes in the LB broth, 38 minutes in the LB plus 1% dextran and 660 minutes in the dextran-salts. The strain did not have ant plasmid, and was susceptible to genramicin, cotrimoxazole and cefoperazone, and moderately susceptible to chloramphenicol, cefamandole and cefotaxime, but resistant to ampicillin, cephalothin, tetracycline, amikacin and tobramycin.

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