• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.514.1-514
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• 1986

토양으로부터 dextranase를 분비하는 균을 분리하였으며, 그 균이 Flavobacterium multivorum으로 동정되었다. 이 F. multivorum의 배치조건에 따른 dextran 이용과 dextranase 분비능을 조사하였고 아울러 성장조건도 알아보았다. 한편 위의 균은 plasmid를 갖고 있지 않았으며, 그에 따라 chromosomal DNA를 추출하여 Sau3Al 절단한 후

• Microbiology and Biotechnology Letters
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• v.23 no.1
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• pp.75-80
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• 1995

Dextranase produced by Flavobacterium multivorum HL-1 isolated from soil were characterized. Optimum growth condition for the production of the enzyme by the strain appeared to be at the 1.0% concentration of dextran. When NH$_{4}$N0$_{3}$ and beef extract was added to the culture media, the enzyme activity increased upto 570 and 680 units per ml respectively, and other nitrogen sources did not increase the activity. Urea, casamino acid, (NH$_{4}$)$_{2}$SO$_{4}$ and KNO$_{3}$ inhibited the activity. In the presence of Mg^{2+} in culture media, the enzyme activity increased 124%, but its activity was inhibited in the presences of Ca$^{2+}$, Co2$^{2+}$, Hg^{2+} and Zn$^{2+}$ . The optimum temperature for the enzyme activity was 45-55$\CIRC$C . In the ranges of pH 4 to 10, the activity of the enzyme appeared approximately similar.

• Korean Journal of Microbiology
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• v.25 no.4
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• pp.346-352
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• 1987

One hundred and seventeen colonies were screened for the detection of the production of exodextranase on the dextran-mineral salts medium. Ten colonies out of them produced the dextranase. Flavobacterium multivorum greatly producing the enzyme was isolated from soil, identified and then studied for various biochemical characteristics. The activity of the dextranase in the cultured medium was high between pH8 and 9 at $35^{\circ}C$, and between $45^{\circ}C$ and $55^{\circ}C$ at pH8. By the growth curves the generation times of the bacterium were approximately 52 minutes in the LB broth, 38 minutes in the LB plus 1% dextran and 660 minutes in the dextran-salts. The strain did not have ant plasmid, and was susceptible to genramicin, cotrimoxazole and cefoperazone, and moderately susceptible to chloramphenicol, cefamandole and cefotaxime, but resistant to ampicillin, cephalothin, tetracycline, amikacin and tobramycin.