• Title/Summary/Keyword: Flat Life

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HeLa Cell Culture on Nanoimprinted Patterns Using Conducting Polymer (전도성 고분자 나노임프린트 패턴 상의 HeLa 세포 배양)

  • Ahn, Junhyoung;Park, Kyungsook;Lee, Suok;Jung, Sanghee;Lim, Hyungjun;Shin, Yong-Beom;Lee, JaeJong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.41 no.1
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    • pp.63-67
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    • 2017
  • In bioscience and biotechnology, the research of fundamental life mechanisms and their diseases caused by insufficiency is important. The study of a whole organism is difficult and sometimes impossible because of DNA, RNA, proteins, cellular organelles, various cells, and organs. Cell cultures can provide a simple method for researching cellular mechanisms and conditions, both in terms of physiological performance, and in response to chemical stimulation. According to conventional cell culture methodology, the flat surface is used with surface treatments for cell adhesion on the surface. Micro- and nanoscale patterns have been developed with chemical and biochemical modifications for cell immobilization. In this study, HeLa cell culture on nanostructures patterns was studied, including the 300 nm line and 150 nm pillar structures, using nanoimprint lithography and pyrrole as a biocompatible conducting polymer.

Lived Experience of Women체s Urinary Incontinence in Small Island (도서지역여성의 요실금 체험)

  • 이명희;신경림
    • Journal of Korean Academy of Nursing
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    • v.30 no.3
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    • pp.799-812
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    • 2000
  • This study adopts the phenomenological approach in order to explore the experience of urinary felt by the small island women and to find the meaning and structure of their experience, for the further understanding of them. This study succeeded in detecting five topics and three basic structure from eight participants, and followings are the comprehensive statement of them. The five topics include neglect of care after childbirth, unavoidable life in the tidal flat, shame which cannot be expressed even to their husbands, endless anxiety toward the expected future, and sad(dilemmatic) lived experience. The basic structure is that small island women who have urinary incontinence are apt to regard their disease as a natural destiny of women who fail to get adequate care after childbirth, and something to be endured to live in the seashore. They think of urinary incontinence as something so shameful that they cannot reveal it even to their husband and family. They believe that it even changes their personality since they must always stay alert in order to cope with the situation; for example, when it takes place unexpectedly, like too often to go to toilet, to change the underwears, to wake up in the middle of the night to go to toilet, to try not to laugh loudly, or to have showers. In addition, they accept it as a natural process of aging and incurable disease, and they consider themselves already ruined on the way of becoming uglier. They show dilemmatic abandonment: give it up unwillingly but at the same time think it is natural for others too. The unique experience of small island women with urinary incontinence implied in those statement are inseparable with the specific conditions for survival in the island. Unlike other diseases, it is considered the result of traditionally poor care after childbirth. However this misunderstanding that it is a natural phenomena for all the women who experience childbirth and aging and thereby incurable leads to an undesirable attitude toward urinary incontinence. According to the analysis, environmental conditions specific for small islands make the women there have distinct and unique experience concerned with urinary incontinence. Consequently, the future nursing plan for urinary incontinence in the small island area must be made and enforced with the consideration of these specific phenomenological meanings. Modern Korean nursing has basically been centered to hospital or urban areas. Besides, nursing intervention has long depended upon the research of western countries. This research, however, shows how greatly the regional and cultural characteristics influence the understanding of a certain disease, and is expected to make more specific and in-depth nursing approach enable for those who have urinary incontinence in small islands.

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Locations and Topographical Character of the MAEULSOOP in the Southwestern and the Eastern Region (마을숲의 분포 위치와 지형적 공간특성 유형화 방안 - 경북 의성, 전북 진안 및 전남 함평지역을 대상으로)

  • Kwon, Jino;Oh, Jeong-Hak;Lee, Jeong-Youn;Park, Chan-Ryul;Choi, Myoung-Sub
    • Journal of the Korean Association of Geographic Information Studies
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    • v.11 no.4
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    • pp.85-93
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    • 2008
  • The MAEULSOOP, Korean traditional village groves have been installed and survived since 7th Century to serve local village dwellers as a community forest. The common sense of their reasons for being is related to the surroundings such as hills, waterways and wind-ways. To understand the roles in a local community, spatial characteristics of distribution, locations and shapes were tested at the two-characterized regions, the Southwestern Flat Region and the Eastern Hilly Region. Approximately more than 500 written evidences related to trees and forests were surveyed, for example village names, folk tales and lists of designated trees for protection. Twenty sites in each region were selected and tested for the spatial analysis. Aerial photographs, DEM and the ArcGIS with a modified AML for slope analysis are applied based on the criteria of the KLCIS(Kwon, 2002; 권진오, 2008). The major factors in the role of the MAEULSOOP based on the spatial character of two regions are; the array and locations of hills for encircling or exposure, locations against corridors and waterway or not, locations of the community to serve, the conservation of energy. Although locations and shapes of the MAEULSOOP are slightly different, it seems that one of the prime roles is what makes their everyday life difficult the most in the community.

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Analysis of Soil Erodibility Potential Depending on Soil and Topographic Condition - A Case Study of Ibang-myeon, Changnyeong-gun, Kyungsangnam-do, South Korea- (토양 및 지형 조건에 따른 토양침식 잠재성 분석 - 경상남도 창녕군 이방면을 대상으로 -)

  • Park, In-Hwan;Jang, Gab-Sue;Lee, Geun-Sang;Seo, Dong-Jo
    • Journal of Environmental Impact Assessment
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    • v.15 no.1
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    • pp.1-12
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    • 2006
  • Changes in the soil physical property and the topographic condition derived from agricultural activities like as farming activities, land clearance and cutting down resulted in environmental and economic problems including the outflow of nutrient from farms and the water pollution. Several theories on the soil conservation have been developed and reviewed to protect soil erosion in the regions having a high risk of erosion. This study was done using the USLE model developed by Wischmeier and Smith (1978), and model for the slope length and steepness made by Desmet and Govers (1996), and Nearing (1997) to evaluate the potential of the soil erodibility. Therefore, several results were obtained as follows. First, factors affecting the soil erosion based on the USLE could be extracted to examine the erosion potential in farms. Soil erodibility (K), slope length (L), and slope steepness (S) were used as main factors in the USLE in consideration of the soil, not by the land use or land cover. Second, the soil erodibility increased in paddy soils where it is low in soil content, and the very fine sandy loam exists. Analysis of the slope length showed that the value of a flat ground was 1, and the maximum value was 9.17 appearing on the steep mountain. Soil erodibility showed positive relationship to a slope. Third, the potential soil erodibility index (PSEI) showed that it is high in the PSEI of the areas of steep upland and orchard on the slope of mountainous region around Dokjigol mountain, Dunji mountain, and Deummit mountain. And the PSEI in the same land cover was different depending on the slope rather than on the physical properties in soil. Forth, the analysis of land suitability in soil erosion explained that study area had 3,672.35ha showing the suitable land, 390.88ha for the proper land, and 216.54ha for the unsuitable land. For unsuitable land, 8.71ha and 6.29ha were shown in fallow uplands and single cropping uplands, respectively.

A Taxonomic Study on Korean Allium L. Based on the Morphological Characters (형태학적 형질에 기초한 한국산 부추속의 분류학적 연구)

  • Choi, Hyeok-Jae;Jang, Chang-Gee;Lee, You-mi;Oh, Byoung-Un
    • Korean Journal of Plant Taxonomy
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    • v.37 no.3
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    • pp.275-308
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    • 2007
  • For 20 taxa of Korean Allium, including 16 species and 5 varieties, examined were morphological characters (i.e. sexuality, structure and shape of underground part, types and growing patterns of leaf and scape, and shapes of perianth, filament and pistil). The specialization and the evolutionary trends of taxonomic characters were inferred from morphological examination. Taxonomic relationships and system of Korean Allium were also studied. The characters showing evolutionary trends were the structure and shape of underground part including rhizome and bulb, leaf, scape, inflorescens, filament and ovary. It seemed that thin and short rhizome developed from thick and long one, and the membranous simple bulb tunic evolved into fibrous reticulate one. The presence of hyaline sheath in A. monanthum of sect. Microscordum was apomorphic. Both angular leaf blade with 2-rowed vascular bundle and flat blade with 1-rowed vascular bundle were developed from terete one with 2-rowed vascular bundle. The base of filament have differentiated from entire to toothed, and 2-ovuled ovary as well as erect scape before flowering was plesiomorphic type. In addition, sexuality, structure of underground part, the presence of hyaline sheath and cross-section structure of leaf were taxonomic characters with the level of the subgenus in this genus. Shape of rhizome, bulb, leaf, scape and pedicel discriminated each section from other ones in the subgenera easily. The shape and arrangement of perianth and filaments were the diagnostic characters for species level along with shape of ovary and stigma.

Studies on the Natural Mortality of the Young Short Necked Clam, Tapes Japonica-II . Resistance to Temperature , Salinity and Exposure for the Young Short Necked Clam (바지락치패의 폐사에 관한 연구-II 바지락 치패의 온도, 염분, 노출에 대한 저항성)

  • CHOE, Sang
    • The Korean Journal of Zoology
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    • v.9 no.1
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    • pp.7-15
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    • 1966
  • In a previous paper, the dirunal and monthly changes of the tidal flat temperature and chlorinity were observed. In this paper the resistance of the young short necked clam in various growth stages to the temperature, to the chlorinity and to the exposure were studies. The young clam used were in early (E), medium (M) and late (L) young stages whose shell lengths were 2.0-3.5mm, 9.0-11.0mm. and 14.0-16.0mm., respectively. The results were : 1. At various sea water temperatures , the chlorinity resistance of the young clam was directly proportional to the shell length. 2. When both inadequate sea water temperature and abnormal chlorinity are simultaneously applied, the resistance of these young clams was more markedly reduced than the case of applying either one of these conditions. 3. In clams of M and L, no lethal effect were observed when daily immersion of four to eight hours for a week into the sea water of any concentration of chloriity at 26-34$^{\circ}C$, whereas in E, 37-90% of mortalities were obtained by immersion of eight hours daily into both the fresh water and the sea water of higher chloriniites (more than 23.5$\textperthousand$ Cl) at the same temperature above. 4. The lower critical thermal maximum for lethal to the young clam was 38$^{\circ}C$. With four hours immersion daily at the water temperature of 38$^{\circ}C$, the mortalities of E, M and L to the lower chlorinities (less than 6.7$\textperthousand$Cl) were 100, 70-100 and 27-37% respectively ; to the higher chlorinities (more than 23.5$\textperthousand$Cl) 10-70, 10-37 and 3 % respectively ; to the normal range of chlorinities (13.4-16.8$\textperthousand$Cl) 0-13, 3 and 0 % respectively. 5. No lethal effects were observed in E and M clams immersed continuously for seven days in sea water with chlornities of 7.2 -21.7$\textperthousand$Cl at 18-24$^{\circ}C$, while notable mortalities were observed in E which had been kept at lower (less than 4.8$\textperthousand$ Cl) and higher (more than 24.1$\textperthousand$ Cl) chlorinites. 6. Although the resistance of the young clam to the chlorinity may have to be related closely to the life history of the clam prior subject to the experiment, the adapted chlorinity range was 7.2-19.3$\textperthousand$ Cl and the optimum range was 13.4-16.8$\textperthousand$Cl. 7. Remarkable lethal effects were observed for the E and M clams to the exposure temperature of 38$^{\circ}C$ whereas the L and had no such fatal results.

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Volatile Compounds and Ultrastructure of Petal Epidermal Cells According to Scent Intensity in Rosa hybrida (장미꽃의 향기정도에 따른 주요성분 및 꽃잎 세포의 미세구조)

  • Lee, Young-Soon;Lee, Yun-Hae;Lim, Seung-Hee;Park, Gun-Hwan;Choi, Sunk-Young;Hong, Hae-Jung;Ko, Jeong-Ae
    • Horticultural Science & Technology
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    • v.31 no.5
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    • pp.590-597
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    • 2013
  • Principle component and emission localization of volatile compounds were investigated according to scent intensity of rose flower. Scent intensity in cultivars and bred-line of Rosa hybrida was divided into three levels; light ('Feel Lip', 'Venus Berry'), medium ('GR07-135'), strong ('Honey Blue'). The major volatile compounds were different depending on the cultivars and selected line; 3,5-dimethoxytoluene (DMT), benzene, 1,3,5-trimethoxy ('Feel Lip'), megastigma-4,6(Z),8(E)-triene ('Venus Berry'), DMT, benzene,1-ethenyl-4-methoxyand, phenylethylalcohol ('GR07-135') and germacrene-D, DMT ('Honey Blue'). The adaxial epidermal cells were conical papillate shape, whereas the abaxial epidermal cells were flat shape. The adaxial epidermal cells of 3 cultivars and 1 selected line were surrounded by thick cell wall and covered by waxy cuticle of 2 cultivars and 1 selected line (except 'Honey Blue'). The adaxial epidermal cells contained starches in 'Feel Lip', osmiophlic droplets in 'Venus Berry', starchs, plastids, vacuoles in 'GR07-135' and plastoglobules, plastids, vacuoles in 'Honey Blue'. Based on these results, it appears that plastids and vacuoles in adaxial epidermal cells with conical papillate shape are associated production and emission of volatile compounds in scent R. hybrida.

Study on the noodle-making properties of rice added with hydroxypropyl methylcellulose (I) (히드록시프로필 메틸셀룰로오스 첨가에 의한 쌀국수 제면성 연구 (I))

  • Yoo, Young-Jin;Kang, Mi-Young;Um, In-Chul
    • Current Research on Agriculture and Life Sciences
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    • v.30 no.2
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    • pp.61-67
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    • 2012
  • In this study, hydroxypropyl methylcelluloses (HPMCs) were added to prepare the rice noodle and the effects of water dosage, HPMC content, and viscosity level of HPMC on the noodle-making properties of rice dough were examined. As the water dosage was increased, the rice dough after roller pass became more flat and homogeneous. However, when the water dosage was over a certain range, the rice noodle became more waved. In the test result of various HPMC contents, 3% HPMC addition was turned out to be an optimum condition for the best noodle-making properties of rice dough. As the viscosity level of HPMC decreased, the rice dough after roller pass became inhomogeneous. However, the lowest viscosity level HPMC (PMC 40H) did not show any waved rice noodle at even high water dosage indicating the improved noodle-making properties of rice dough. On the whole, it seems that 56~58% water dosage, 3% HPMC content, and high viscosity HPMC (PMC60U) were the optimum condition for preparation of good rice noodle.

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A Study on Measurements of Autoignition and Activation Energy of Superabsorbent Polymers (고흡수성 중합체의 자연발화와 활성화에너지 측정에 관한 연구)

  • Jong-Man Heo;Jae-Wook Choi
    • Journal of the Society of Disaster Information
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    • v.19 no.2
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    • pp.292-304
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    • 2023
  • Purpose: This study was conducted to obtain experimental data for the establishment of preventive measures against fire, as large and small fire accidents occur at production and storage sites of superabsorbent polymers developed for the convenience of daily life. Method: The sample container was fixed at 0.2m in both length and width, and was shaped into a rectangular cuboid with heights of 3cm, 5cm, 7cm, and 14cm to access an infinite flat plane. The sample container was fixed in the center of a thermostatic bath that was heated to a predetermined temperature according to a preset temperature control program. If the central temperature of the sample rose more than 20℃ above the set temperature, it was determined to have 'ignited', and if it remained similar to the set temperature, it was determined to have 'unignited'. Result: The critical autoignition temperature was calculated to be 212.5℃ for a sample container with a height of 3cm, 202.5℃ for 5cm, 192.5℃ for 7cm, and 177.5℃ for 14cm. The ignition induction time to reach the highest temperature was approximately 42hours for 3cm, 91hours for 5cm, 151hours for 7cm, and 300hours for 14cm. Conclusion:① As the size of the sample container increased, the autoignition temperature decreased and the ignition induction time to reach the highest temperature increased. ② The apparent activation energy was calculated to be 39.30kcal/mol, with a correlation of 99.5%.

Experimental Study on Autoignition of Superabsorbent Polymers (고흡수성 중합물질의 자연발화에 대한 실험적 연구)

  • Jong-Man Heo;Jae-Wook Choi
    • Journal of the Society of Disaster Information
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    • v.19 no.2
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    • pp.280-291
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    • 2023
  • Purpose: As fire accidents happen at the production and storage sites of superabsorbent polymers for convenience of daily life, an experimental study was conducted to secure basic data to establish practical preventive measures against them. Method: The sample container (20cm width × 20cm length) was made into a rectangular cuboid with the heights of 3cm, 5cm, 7cm, and 14cm, respectively, to allow access to the infinite flat plane. The front and back of the container were covered with a 300-mesh stainless steel mesh for one-dimensional heat transfer. The sample container was placed in the center of the thermostatic bath, which was heated to a predetermined temperature by setting the thermostat program in advance, and it was determined to be 'ignited' when the central temperature of the sample rose by more than 20℃ above the set temperature, and "unignited" when it was maintained at an approximate value of the set temperature. Result: The critical autoignition temperature was calculated to be 217.5℃ when the height of the sample container was 3 cm, 212.5℃ when it was 5 cm, 202.5℃ when it was 7cm, and 187.5℃ when it was 14cm. The ignition induction time to reach the maximum temperature was 34hours for 3cm, 76hours for 5cm, 143hours for 7cm, and 318hours for 14cm. Conclusion: ① As the size of the container increased, the autoignition temperature decreased and the induction time to reach the maximum temperature increased. ② An apparent activation energy was calculated to be 44.92kcal/mol, with a correlation of 96.93%.