• 대한수의학회지
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• 제15권1호
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• pp.57-67
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• 1975

The poxvirus group is considered to be a typical cytoplasmic inclusion forming virus. Every poxvirus has been reported to produce only one kind of inclusion in the infected tissues. A vague concept that inclusions of poxviruses are eosinophilic or acidophilic has prevailed. Although many papers and theories about the nature of the inclusion have been presented, most of them are not quite convincing on the point of the relations with virus multiplication, and an analysis of papers published showed that there seem to be many discrepancies in the descriptions of the nature of the poxvirus inclusions. Comparative studies on host-virus interaction with cowpox, orf, swinepox and fowlpox viruses which selected from each Group (I-IV) of poxviruses were performed from the morphological and virological standpoints. At first, in cowpox virus-FL cell system, as a comparative model, cytoplasmic inclusion, nucleic acid metabolism by autoradiography and detection of viral antigen by immunofluorescence were studied and obtained the results as follows: 1. The focus-like cytopathic effect (CPE) at early stage developed to entire culture at terminal stage of infection, and also the developing status of CPE was correlated to viral doses for inoculation. Two kinds of cytoplasmic inclusions which named A and B type were easily observed by Giemsa, hematoxylin-eosin (H & E) and May-Greenwald Giemsa (MGG) stainings in the infected cells. The B type inclusions were formed at early stage of infection and the A type inclusions were produced subsequently the B type formation. The B type which common type inclusion in poxviruses was a small compact or aggregate at early stage and developed to a large diffuse body at terminal stage of infection. On the other hand, the A type inclusion which depend upon the kind of virus was appeared as round and discrete shape, and its size and number was increased gradually during the culture period. It was characteristic to form distinct halos around the both types of inclusions in acid fixed, H & E stained preparations of infected cultures. The B type inclusion was always positive in Feulgen reaction and showed as DNA containing body but the A type inclusion was not. 2. In the relationship between inclusion and DNA metabolism of infected cells by the qualitative autoradiography using 3H-thymidine, the appearance of silver grains was coincided with B type inclusion but not with A type inclusion. This showed that the DNA synthesis was proceeded in all B type inclusions except those in the terminal stage with a diffuse form. This suggested that the B type inclusions are only sites of DNA synthesis and this was proceeded after the cell infection independently. The activity of DNA synthesis of the inclusions was nearly the same as that of the nucleic of normal cells and non-inclusion bearing cells. and non-inclusion bearing cells. Regardless of the size of the degree of DNA synthesis of the B type inclusion, inclusion bearing cells all showed remarkable suppression of nuclear DNA synthesis. 3. By the direct fluorescent antibody technique viral antigen in infected cells was detected. The B type inclusions have been proved to contain a great deal of viral antigen, whereas the basic substance of A type inclusion did not show antigenicity except the round edge. It was suggested that the round edge fluorescence might be caused by the glare of cytoplasmic viral antigen which pushed out and concentrated by the A type inclusion development. 4. Hemorrhagic red pock formations on chorioallantoic membrane of embryonated chicken egg had proved the characteristic of used viral strain. 5. By the above studies on the nature of two types of inclusions and the role they play in virus multiplication, it was concluded that the B type inclusion must be the site of the synthesis of viral DNA and protein as well as the site of the virus.

• 임산에너지
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• 제7권1호
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• pp.22-27
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• 1987

신품종 포플러류의 식재(10,000본/ha) 당년생일 때 직경, 수고, 재적$(D^2H)$등 몇가지 조사특성에 의한 biomass 생산량과 생산량 추정식을 유도한 결과를 요약하면 다음과 같다. 1. 현사시의 평균 건물 생산량은 줄기 2.86, 가지 0.41, 잎 0.70, 전체가 3.97t/ha이었으며 건중량 대 생중량의 비는 줄기 0.47, 가지 0.36, 잎 0.31, 전체가 0.41로 나타났다. 양황철나무의 경우 평균 건물생산량은 줄기 1.48, 가지 0.54, 잎 0.71, 전체가 2.73t/ha이었으며 건중량 대 생중량의 비는 줄기 0.42, 가지 0.37, 잎 0.28, 전체가 0.36이었다. 2. Biomass 생산량(건중량 및 생산량) 추정은 현사시의 경우 직경만으로 비교적 정확히 추정할 수 있었지만 양황철나무의 경우 직경 식재 당년생에서는 어느 특성으로도 추정이 어려울 것으로 생각된다. 3. Biomass 생산량(건중량) 분포비율은 현사시의 경우 줄기, 가지, 잎이 각각 73, 10, 17$\%$로 나타났으며 양황철나무는 줄기, 가지, 잎이 각각 54, 20, 26$\%$로 나타나 줄기 건중량에 의한 가지, 잎, 전체 건중량을 추정할 수 있었다.

• 한국음향학회:학술대회논문집
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• 한국음향학회 2000년도 하계학술발표대회 논문집 제19권 1호
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• pp.325-328
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• 2000

According to H.B.Lee(1993), standard Korean vowel qualities are as follows: in /i/, /e/, $/\epsilon/$, /a/, /o/, /w/, they have 4 qualities each other and in /er/ there are 3 qualities. The environments of 4 qualities are iong and stressed vowel in word initial, short and stressed vowel in word initial, unstressed vowel in word initial, unstressed vowel in word finial. The aim of this study is to seek and compare with H.B.Lee(1993). Conclusively I could not find on the whole any pattern of the same types of H.B.Lee(1993) in this study And especially in Fl vowel formant values of /er/and /w/, I never found any pattern of the same types of H.B.Lee(1993). Also F2 vowel formant values of $/\varepsilon/$ and /w/ do not have any kind of pattern of the same types of H.B.Lee(1993), between them, the patternize of F2 vowel formant values in /w / is especially difficult. It is the same story of Jaekang Lee(1998). But in some case, the patternize could be done. among the whole vowels, analysis environment b has the wide width on the change of the formant value. As the another result of the analysis It is to possible to make the pattern of the old male group. The old male group on the whole is analyzed to have the most low formant values and the old women group is analyzed to have the most high formants values, but in the most high formant valus there are young women group. And the formant values's rising in 2 cases of the formant value of /er/ is analyzed to have the same pattern of H.B.Lee(1993).

• 한국미생물·생명공학회지
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• 제34권1호
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• pp.7-14
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• 2006

가금류의 feather은 전 세계적으로 발생되지 않는 지역이 없을 만큼 광범위하게 발생되어지는 폐기물로써, 현재까지 많은 연구자들에 의하여 그 이용성에 대한 연구가 활발하게 이루지고 있다. 그러나 disulfide 결합, 수소결합 및 이온결합에 의하여 매우 강한 결합력을 가지는 feather 구성 단백질의 화학적인 성질 때문에 분해하여 사용하기에 많은 어려움이 있다. 가금류 폐기물을 이용한 사료의 제조과정에서 가열 및 가압 등의 과정을 거치면서 아미노산의 손실이 발생하고 낮은 소화율 등이 문제로 야기되어지고 있다. 이러한 문제를 해결하기 위한 노력으로 미생물이 생산하는 keratinolytic protease를 이용하고자 산업적으로 이용 가능성을 가지는 균주를 분리하였다. 토양 시료로부터 Bacillus sp. SMMJ-2, FL-3, NO-4 및 RM-12 4종의 keratinolytic protease 생성균을 분리하였다. 이 균주들은 5.0% skim milk agar plate에서 높은 protease 활성을 나타내었으며, 2.0% whole chicken feather agar plate에서도 많은 점질성의 물질을 생산하였다. Keratinolytic protease 생산을 위하여 배지4($0.7%\;K_{2}HPO_{4},\;0.2%\;KH_{2}PO_{4},\;0.1%$ fructose, 1.2% soybean meal, $0.01%\;Na_{2}CO_3$, pH 7.0)에서 Bacillus sp. SMMJ-2에 의한 keratinolytic protease의 생산을 조사한 결과, 배양 3일에 가장 높은 keratinolytic protease 활성을 나타내었다. 그러나, Basal medium(0.05% NaCl, $0.03%\;Na_{2}HPO_{4},\;0.04%\;NaH_{2}PO_{4},\;0.5%$ whole chicken feather, pH 7.0)에 유일한 탄소 및 질소원으로 whole chicken feather를 첨가하고 배양한 결과, $30^{\circ}C$에서 일주일 이상 배양하였을 때 feather은 모두 분해되었으나 깃대는 분리하지 못하였고 배지 4에 질소원으로 1.2% whole chicken feather를 첨가하였을 때 40시간 이내에 깃대를 포함한 feather를 모두 분해하였다. Bacillus sp. SMMJ-2에 의한 keratinolytic protease의 생산은 접종 후 9시간이 지나면서 생산되기 시작하여 24시간 동안 배양하였을 때 최대 활성의 86%를 나타내는 것으로 조사되었다. Bacillus sp. SMMJ-2에 의하여 생산되어지는 keratinolytic protease 활성은 $30^{\circ}C$, 180 rpm으로 3일간 배양했을 때 106 units/ml/min 이였으며, protease 활성은 540 units/ml/min을 나타내었다. 온도와 pH에 대한 효소의 안정성은 $50\%$ acetone을 이용하여 분리한 효소로 조사한 결과, $30{\sim}50^{\circ}C$까지는 80% 이상의 잔존효소활성을 나타내었고, $60^{\circ}C$ 이상에는 20분간 열처리로 효소가 거의 실활 되었다. 효소의 pH 안정성은 pH $6.0{\sim}12.0$에서는 비교적 안정한 것으로 조사되었다.

• Journal of Acupuncture Research
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• 제23권6호
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• pp.153-164
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• 2006

Introduction : The Aim of the Study Is to Investigate the Characteristics of Yangdorak m the Patients with Idiopathic Parkinson's Disease. Methods : Subjects Were Voluntarily Recruited by Newspapers And Internet. All the Subjects Were Confirmed as Idiopathic Parkinson's Disease by a Neurologist. The Patient's Symptoms Were Assessed by Unified Parkinson's Disease Rating Scale (UPDRS), Hoehn-Yahr (H-Y) Stage, Schwab & England Activity of Daily Living And Freezing of Gait Questionnaire(FOGQ). Sasang Constitution Was Differentiated by QSCCII. Yangdorak Was Measured after 15 minutes' Bed Rest Results : The Results Were as Follows; 1. The Average Value of Yangdorak m 50 Patients with Parkinson's Disease Was $39.01{\pm}17.97$. 2. The Mean Values of Fl, F2, F3, F4, F5 and F6 were under 40. 3. The Number of Pyesaek Was Significantly Increased According to H-Y Stage. 4. The Yangdorak Value Had No Significant Correlation between UPDRS, H-Y Stage and Duration. 5. There Was No Significant Difference in the Value of Yangdorak among Sasang Constitutions. Conclusion : This Study Suggests that Yangdorak Can Be Used as a Assistant Tool to Investigate the Patients of Parkinson's Disease. Further Study on the Yangdorak And Parkinson's Disease Is Recommended.

• Design & Manufacturing
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• 제14권3호
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• pp.17-22
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• 2020

As devices such as smartphones, tablet PC, and wearable devices have been miniaturized, the connectors that go into the devices are also designed to be very small. The connector combines the plug and the receptacle to transfer electricity. As devices are miniaturized, the contact shape is formed by partially thinning the thickness of the raw material of the terminal in order to lower the coupling height of the plug and receptacle. The product used in this study is a receptacle terminal used for 0.4mm pitch board to board connector among fine pitch connectors. When the material thickness is reduced by forging the receptacle terminal, the width change of the pin is checked. To reduce the thickness of the material by forging, pre-notching is applied in the first step, forging in the second step, and notching in the third step. After forming the width dimension of the pin to 0.28 mm in the pre-notching process, in the forging process, the material thickness 0.08 mm and 0.02 mm (25%) were forged and the thickness was changed to 0.06 mm and the width change amount of the pin was measured. The facility produced 10,000 pieces at 400 SPM using a Yamada Dobby (MXM-40L) press, and thirty pins were measured and the average value was shown. After forging by using C5210-H (HP) and NKT322-EH, which are frequently used in connectors, analyze the amount of change in each material. The effect of punching oil on forging is investigated by appling FM-200M, which is highly viscous, and FL-212, fast drying oil. This study aims to minimize mold modification by predicting the amount of material change after forging.

• 한국대기환경학회지
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• 제10권1호
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• pp.57-63
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• 1994

In order to comprehension of the behaviour of PAHs in air which is known as carcinogens and/or mutagens suspended Particulates in ambient air were collected by Anderson air sampler from 1992. 6 to 1993. 5 in Seoul. Ten species of PAHs( Phen, An, Fl, R, Py, BaAn, BaP, Chry, BeP, DiB(a, h)An, I123p) were analyzed to understand monthly variations of PAHs distribution of PAHs concentration according to particle size, and correlation between PAHs and independent charactierstics of PAHs The highest concentration of TSP was 155.58$\mu\textrm{g}$/㎥ in May and the lowest was 60$\mu\textrm{g}$/㎥ in August. Concentration of TSP was more affected by coarse particles in spring, otherwise which was more affected by fine particles in winter. According to results of anaylsis of samples that were collected by Anderson air sampler, concentration of PAHs was more high in winter than that in summer. In almost samples collected by Anderson air sampler, concentration of PAHs was more high in coarse particles than in fine particles, but BaP well known as carcinogenic matter had more high concentration in fine particles(56-97.5%) than that in coarse particles(2.5-46%). Correlation between concentrations of TSP and PAHs was more high in fine Particles than in coarse Particles. Both fine particles and coarse particles have negative correaltion with radiation.

• 한국수정란이식학회지
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• 제10권2호
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• pp.163-169
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• 1995

In the experiment I for maas production of bovine early embryos, 18~20hpi fertilized eggs (756 eggs) and parthenogenic eggs (618 eggs) which were treated by 10% ethanol were cultured in both TGM and CZB. In the experiment II, suppiment effects each in CZB and CRlaa were tested by matured and fertilized oocytes which were after 18~20hpi. In the case of experiment I after 48hr, the cleavage rates of normally fertilized eggs were 66.6% in TCM treatment and 77.7% in CZB treatment, and after 240h the blastocysts were 7.5% in TCM and 14.1% in CZB. In the parthenogenic eggs, the deavage rates at 48hr were 39.6% in TCM and 57.5% in CZB, and at 240h, the blastocysts were 0.9% in TCM and 4.4% in CZB. These results showed that the effects of CZB on developmental ability to parthenogenic eggs as well as nomally fertilized eggs are relatively high. In experiment W, the effect of exposing the cleaved embryos to CZB for 30h on the blastocyst formation was examined. Similar rates of blastocyst formation were obtained both in TCM and CZB, suggesting that CZB exposure. during ealry development is critical. In experiments III ~ V, the effects of supplements were examined. The cleavage rates of CZB treatments at 48h were 83.8% in control, 78.1% in BSA+A.A+SIT, 75% in 5% FCS+A. A+SIT, 88.6% in BSA+A.A+SIT and not co-cultured BSA+A.A+SIT had 85.7% and in the case of 240h blastocysts showed 22.6, 0.0, fl.1, 6.5 and 0%, respectively. As a result, this study showed that CZB was effective culture system for in vitro development, and that CZB and CR$_1$aa had no significant differences and effects between them. It may be concluded that in the simple media containing supplements could replace the co-culture systems of bovine early embryo development.

• 한국패류학회지
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• 제28권3호
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• pp.255-258
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• 2012

표본은 2012년 3월 경상북도 독도주변해역에서 자망에 의해 어획된 것이다. 화살꼴뚜기는 몸통길이 (ML) 22.5-34.2cm (평균 $27.1cm{\pm}2.8$) 이다. 지느러미길이 (FL) 는 몸통길이 (ML) 의 2/3정도인 60%를 차지하였다. 지느러미폭(FW) 은 몸통길이 (ML) 의 약 1/2정도이다. 팔은 몸통길이(ML) 에 비해 짧고 굵으며, 팔길이식은 III>II>IV>I 이었다. 왼쪽 4번째 팔의 말단부 약 30%가 교접기로 되어 있다. 교접기는 아주 작은 흡반 및 거의 흔적적인 흡반이 나열되어있다. 표본 44개체 중 암컷 (♀) 은 23개체, 수컷 (♂) 21개체이었다. 생식소 발달단계는 암컷 (♀) 완숙단계, 수컷 (♂) 방정단계였다.

• 대한음성학회지:말소리
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• 제25_26호
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• pp.3-26
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• 1993

The goals of the present study were (3) to collect and analyze sets of fundamental frequency (F0) and formant frequency (F1, F2, F3) data of Korean diphthongs from ten linguistically homogeneous speakers of Korean males, and (2) to make a comparative study of Korean monophthongs and diphthongs. Various definitions, kinds, and previous studies of diphthongs were examined in the introduction. Procedures for screening subjects to form a linguistically homogeneous group, time point selection and formant determination were explained in the following section. The principal findings were as follows: 1. Much variation was observed in the ongliding part of diphthongs. 2. F2 values of (j) group descended while those of [w] group ascended, 3. The average duration of diphthongs were about 110 msec, and there was not much variation between speakers and diphthongs. 4. In a comparative study of monophthongs and diphthongs, Fl and F2 values of the same offgliding part at the third time point almost converged. 5. The gliding of diphthongs was very short beginning from the h-noise. Perceptual studies using speech synthesis are desirable to find major parameters for diphthongs. The results of the present study wi11 be useful in the area of automated speech recognition and computer synthesis of speech.