• Fisheries and Aquatic Sciences
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• 제6권2호
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• pp.45-50
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• 2003

Food garbage fermented with microbial starter was formulated to diet for the growth of juvenile flounder (Paralichthys olivaceus). Two replicate groups of fish, an average weight of 4.0g, were fed the four isocaloric (19.5 MJ/kg diet) diets with different fermented food garbage levels $(0,\;5,\;10\;and\;15\%)$ for 45 days. Survival, feed efficiency, hepatosomatic index and protein efficiency ratio of fish were not affected by dietary fermented food garbage level (P>0.05). Weight gain of fish fed the diets with 5, 10 and $15\%$ fermented food garbage was significantly higher than that of fish fed the control diet (P<0.05). Condition factor of fish fed the diet with $10\%$ fermented food garbage was significantly higher than that of fish fed the control diet (P<0.05). Daily feed intake of fish fed the diets with 5 and $15\%$ fermented food garbage was significantly higher than the control diet (P<0.05). Proximate composition of whole body and plasma glucose concentration were not affected by dietary fermented food garbage level (P>0.05). These findings indicate that fermented food garbage could be utilized as a feed ingredient for juvenile flounder.

• 한국양식학회지
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• 제13권1호
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• pp.1-7
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• 2000

The effect of various dietary protein levels on growth and body composition of young common carp raised from 56 g to 170 g in recirculating system was investigated for 15 weeks when they were fed to visual satiety three times daily. Five experiemtal diets were formulated to contain 40, 35, 30, 25 and 21% protein levels and 3.56, 3.59. 3.63, 3.66 and 3.69 kcal/g diet GE levels respectively. Mean survival rates of the fish fed the 40, 35, 30 and 25% protein diets were not different but sig-nificantly higher than that of the fish fed the 40, 35, 30 and 25% protein diets were not different but sig-nificantly higher than that of o the fish fed the 21% protein diet(P<0.05) Weight gain (g/tank) of common carp fed the 30% protein diet was the best. However weight gain of the fish fed the 25, 30, 35 and 40% protein diets were not different but significantly better than that of the fish fed the 21% protein diet. Feed efficiency ratio of the 21% protein diet was significantly lower (P<0.05) than for other groups of diets which were not different among them. Protein efficiency ratio for the 21% protein diet was significantly lower (P<0.05) than for other groups of diets which were not different among them(P>0.05) Dietary protein level had no effect on hemoglobin content in the fish(P>0.05) Crude protein contents of whole body of the fish fed the 35 and 40% protein diets were significantly higher than that of the fish fed the 21$$\mid$% or 25% protein diet(P<0.05) Body crude lipid contents of the fish fed the 21 and 25% protein diets were significantly higher than that of the fish fed the 30% or 35% protein diet. Crude ash contents of the fish fed the 35 and 40% protein diets were significantly higher than that of the fish fed the 21% or 25% protein diet(P<0.05) Moisture content of the fish fed the 35% protein diet was significantly higher than that of the fish fed the 21% protein diet(P<0.05) In considering growth performance of common carp and efficiency of diet dietary protein level could be lowered up to 25% without the reduction of young common carp production in recirculating system.

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.565-575
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• 2009

Two experiments in the sequential order were conducted to determine the effects of different dietary lipid sources on the growth and fatty acid composition of rohu (Labeo rohita) and to examine the viability of a return fish oil finisher diet in restoring the human cardio-protective fatty acid profile. In the first experiment, fish were fed either with coconut oil (D1), olive oil (D2), sunflower oil (D3), linseed oil (D4) and fish oil (D5) as the main lipid source in the isonitrogenous diet for 90 days. No significant differences in growth were observed. Among the experimental diets moisture content of fish varied significantly (p<0.05) between the groups. Dietary lipid sources had a profound influence on the fatty acid profile of the muscle and liver as tissue fatty acid profile reflected the dietary fatty acid composition. Increased amounts of eicosapentaenoic acid and docosahexaenoic acid were observed in tissue of fish fed D4 and arachidonic acid was observed in the tissue of fish fed D3. We have also detected the metabolites of n-3 and n-6 pathway in D4 and D3 groups respectively, which prompted us to conclude that rohu, can desaturate and elongate $C_{18}$ essential fatty acids to $C_{20}$ and $C_{22}$ HUFA. A second feeding trial was conducted using the animals from the five different treatment groups for the duration of 30 days with fish oil rich diet (D5). Feeding with fish-oil rich washout diet resulted in the near equalization of all the other treatment groups tissue fatty acid profiles to that of fish oil (D5) fed group. These results indicate that a finishing fish oil diet can be effectively used to restore the human cardioprotective fatty acid profile in rohu fed with vegetable oils as lipid source.

• Journal of Nutrition and Health
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• 제38권4호
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• pp.297-306
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• 2005

It is known that dehydroepiandrosterone (DHEA) shows a dual effect, prooxidant or antioxidant, depending on the do-sage or physiological status of animals. The purpose of this study was to determine the effects of DHEA administration at low dose on lipid peroxidation, protein carbonylation and fatty acid composition in liver. Sprague Dawley male rats were fed either com oil diet containing $15\%$ com oil or fish oil diet containing $2\%$ corn oil + $13\%$ sardine oil, with or without $0.2\%$ DHEA for 9 weeks. Atherogenic index and hepatic triglyceride and cholesterol levels were significantly reduced by DHEA administration in rats fed with fish oil diet. Hepatic lipid peroxide product (TBARS) and protein carbonyl levels were significantly higher in rats fed with fish oil diet than in rats fed with corn oil diet. However, DHEA administration significantly reduced the hepatic thiobarbituric acid-reactive substance (TBARS) and conjugated diene levels in rats fed with fish oil diet. Contents of C16 : 0, C16 : 1, C20 : 5 and C22 : 6 in hepatic microsome were higher in rats fed with fish oil diet than in rats fed with corn oil diet, and contents of C18 : 2 and C20 : 4 were lower than in rats fed with com oil diet. DHEA administration significantly increased C16 : 0 and C18 : 3 contents and reduced C18 : 2 content in rats fed with com oil diet, while it increased C16 : 0 and C18 : 1 and reduced C20 : 5 and C22 : 6 in rats fed with fish oil diet. On overall, DHEA administration increased saturated fatty acid (SFA) and reduced polyunsaturated fatty acid (PUFA) in hepatic microsome, thereby PUFA/SFA ratio was significantly (p < 0.0001) reduced without the change of n-3/n-6 ratio. Taken together, low dose of DHEA administration lowered PUFA/SFA ratio in hepatic microsomal membranes and also showed antioxidative effect especially in fish oil-induced highly oxidative stress condition through blocking increases of C20 : 5 and C22 : 6 contents.

• 한국수산과학회지
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• 제32권2호
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• pp.149-154
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• 1999

이 연구는 16주 동안 치어기 조피볼락 사료에 있어서 어분단백질에 대한 어분대체품의 이용 가능성을 결정하고자 실시하였다. 어분대체품 (FMA)은 혈분 (BM), 오징어간분 (SLP), 육골분(MBM), 수지박 (LM), 가금부산물 (PBP) 및 우모분 (FM)등으로 제작$\cdot$사용하였고, 필수아민노산 (EAA) 중 2가지 (Methionine& Lysine)를 첨가하여 사용하였다. 5가지의 실험사료는 조단백질 $50\%$로, 가용성 에너지 15.6KJ/g으로 조정하였다. 실험사료는 조단백질을 기준으로 어분단백질과 어분대체품 비율을 다음과 같이 요약하였다 :사료 1, $100\%$ WFM: 사료 2, $87.5\%$ WFM : $12.5\%$ FMA, 사료 3, $75\%$ WFM : $25\%$ FMA;사료 4, $75\%$ WFM : $25\%$ FMA+2 EAAs; 사료 5, $50\%$ WFM : $50\%$ FMA+2 EAAs (Methionine& Lysine). 증체율, 사료효율, 일간성장률 및 단백질 전환효율에 있어서 사료 2 ($12.5\%$ FMA)는 사료 1 (대조구)에 비해 유의적인 차이가 없었지만 (P>0.05), 나머지 사료구들은 대조구에 비해 유의적으로 낮게 나타났다 (P>0.05). 필수아미노산을 첨가한 사료 3 ($25\%$ FMA)과 사료 4($25\%$ FMA+2EAAs)의 두 사료구간에서는 사료구 4가 유의적으로 높은 경향을 보였다(P<0.05). 따라서, 치어기 조피볼락에 있어서 필수아미노산 첨가없이 어분단 백질을 복합 어분대체품 (FMA)으로 $12.5\%$ 정도 대체 가능함을 알 수 있었고, 2개의 필수아미노산의 첨가는 어분대체품에 사용되었을 경우에는 어느 정도 효과가 나타났다.

• Fisheries and Aquatic Sciences
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• 제22권4호
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• pp.8.1-8.9
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• 2019

A 12-week feeding trial was designed to evaluate the effect of total replacement of fish oil (FO) with terrestrial alternative oils on growth, feed utilization, body composition, hematological parameters, and fillet fatty acid profile of mandarin fish juveniles. Four iso-nitrogenous (56% crude protein) and iso-lipidic (13% crude lipid) practical diets were formulated. A control diet contained 6% FO and three other experimental diets were prepared by replacing FO with linseed oil, soybean oil, and lard (designed as FO, LO, SO, and lard, respectively). Each diet was randomly allocated to triplicate groups of 25 fish ($1.8{\pm}0.03g/fish$) in a circular tank. Complete replacement of FO by three tested alternative oils had no remarkable impact on growth performance, feed utilization efficiency, and morphological and hematological parameters of juvenile mandarin fish. However, daily feed intake was found to be significantly higher for fish fed the SO diet compared with those fed the FO and LO diets. Fish fed LO and SO diets exhibited significantly higher levels of the whole body lipid compared to fish fed diet containing FO. Fillet fatty acid composition reflected dietary fatty acid profile. The highest level of ${\alpha}$-linolenic acid, linoleic acid, and oleic acid was observed in fish fillet fed LO, SO, and lard, respectively. Although the eicosapentaenoic acid level of fish fillet fed diet FO was higher than other treatments, no significant difference was found in docosahexaenoic acid content among all dietary groups. The results of the present study clearly demonstrate that the complete replacement of FO in mandarin fish diets is achievable. These findings are useful in dietary formulation to reduce feed costs without compromising mandarin fish growth.

• Fisheries and Aquatic Sciences
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• 제12권4호
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• pp.305-310
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• 2009

This study was carried out to evaluate the use of fermented skipjack tuna viscera (FSTV) as an alternative for fish meal in juvenile olive flounder (Paralichthys olivaceus) diets. Lactobacillus bulgaricus was used as a starter for fermentation of skipjack tuna viscera. Four isonitrogenous (49% crude protein) and isocaloric (4 kcal/g DM) diets were formulated to contain graded levels (0, 5, 10, and 15%) of FSTV. Each experimental diet was fed three replicate groups (40 fish per tank) of juvenile flounder (average weight, $3.3\pm0.2$ g) for 5 weeks. At the end of feeding experiment, inclusion of FSTV up to 15% in diets did not affect survival rate (%) and weight gain of fish. Feed efficiency, protein efficiency ratio, protein and lipid retentions of fish fed the diet containing 10% FSTV were higher than those of fish fed the control diet (P<0.05). The values of fish fed the diet containing 15% FSTV were not different from those of fish fed other diets. Whole body lipid content of fish fed the diet containing 10% FSTV was higher than that of fish fed the diet containing 15% FSTV and control diet. The present results indicate that fermented skipjack tuna viscera could partially replace fish meal in juvenile flounder feed, and the inclusion of 10% FSTV may be efficient in improving the feed utilization of fish.

• Preventive Nutrition and Food Science
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• 제21권3호
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• pp.281-288
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• 2016

This study was designed to investigate the effects of dietary astaxanthin levels on growth performance, feed utilization, muscle pigmentation, and antioxidant capacity in juvenile rainbow trout. Four experimental diets were formulated to contain 0, 50, 75, and 100 mg/kg astaxanthin (designed as AX0, AX50, AX75, and AX100). Each diet was fed to triplicate groups of fish (18.5 g/fish) for 10 weeks. Growth performance and muscle composition of fish were not affected by dietary astaxanthin levels. Total carotenoid concentration in the muscle of fish fed the AX50 diet was higher than that of fish fed the AX0 diet, but no significant differences were observed between these fish and those fed the AX75 and AX100 diets. Muscle astaxanthin content increased with increased astaxanthin in the diet. Deposition of astaxanthin in the flesh resulted in a decrease in lightness and an increase in redness and yellowness. The fillets from trout fed the AX75 diet had significantly lower lightness than trout fed the AX50 and AX100 diets. Fish fed the AX50 and AX75 diets showed significantly lower catalase activity than those fed the control diet. Total antioxidant status increased significantly in all astaxanthin supplemented groups when compared to the control group. Superoxide dismutase activity was significantly decreased in fish fed the AX50 diet compared to fish fed the AX0 diet. These findings suggest that while fillet pigmentation increased with increasing dietary astaxanthin concentration, indices of fish antioxidant capacity may not be affected in a dose dependent manner.

• 수산해양교육연구
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• 제22권4호
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• pp.529-536
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• 2010

This study was conducted to effect of extruded pellets (EPs) and moist pellet (MP) diet on growth and fish health parameters in farm cultured olive flounder Paralichthys olivaceus. Two replicate groups of 2,600 fish per each tank (initial weight of $30.1{\pm}0.2$ g) were fed one of the three EPs (EP1, EP2 and CEP) and one MP for 13 months. In field feeding experiment conducted in commercial flounder farm, survival, growth (30-600 g) and condition factor of fish fed experimental EP1 and EP2 were comparable to those of fish fed MP, but considerably higher feed efficiency and protein efficiency ratio were observed in fish fed experimental EPs. Viable cell counts from diet and water from MP diet were considerably higher than those from EP diet, and lysozyme activity from EP diet was higher than that from MP diet. GOT and GPT of fish fed the EP diet were considerably lower than those from MP group, but total protein and glucose concentration of fish fed the EP groups were not significantly different from that of fish fed the MP. Therefore, these results strongly suggest that EP diets could be developed to replace MP diet without adverse effects on growth performance and to enhance the immunity and protective ability of olive flounder against fish disease such as Vibro, Edwardsiella and Strptococcus.

• 수산경영론집
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• 제29권1호
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• pp.121-137
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• 1998

This study was conducted to examine the economic evaluation of the newly developed fish meal analog (BAIFA-M) in Korean rockfish feed. A raw fish feed (MP) and two commercially formulated diets (EP) were employed to compare weight gain (WG), feed conversion ratio (FCR) and survival rate (SR) from the sea cage culture system. By using the economic model in the practical sea cage culture system, feed cost, production cost and gross profit per kg production, rate of profit to gross revenue (RPGR), and internal rate of return (IRR) were calculated based upon the results of the experiment and the information of the private aquaculture farm (Young Chang) in Tong young, Korean. IRR criteria is one of the popular economic feasibility analysis methods applicable far aquaculture industry. This is an economic evaluation method to compare the given interest rate or the discount rate with IRR which can be calculated by the difference between the present value of the benefit stream and of the cost stream. The benefits of using EP on WG, FCR, SR, and production cost will be emphasized in this study. Fish averaging 20$\pm$3.6g (Mean$\pm$SD) were randomly distributed in each small cage (6m$\times$6m) as groups of 2,000 fish. By using 3 large size cages (12m$\times$12m), 12 small cages were constructed, and only 9 small cages were employed for three replicates of each diet treatment. To compare with MP diet, two sinking EP diets were designed by our laboratory and produced by the local feed company who wanted to promote these EP diets for the mass cage culture of Korean rockfish in the future. Two EP diets contain white fish meal and/or BAIFA-M as the main animal protein sources : WFM diet, maximum 43% of white fish meal : BAIEA- M diet, 30% of white fish meal nab replaced by BAIFA- M from WFM diet. Results are summarized in Table 1. Fish fed MP diet showed significantly lower SR than does fish fed two EP diets(P<0.05). However, there Were no significant difference on FCR among fish fed three practical diets. Table 1. Average feed conversion ratio (FCR), accumulative average survival rate (SR) and economic evaluation data far three practical diets. As we expected, BAIFA-M diet is more economical than WFM diet as well as MP diet. Feed cost and production cost per kg production from BAIEA - M diet were lower than those from WFM and MP diets. Moreover, gross profit per Kg production, RPGR and IRR from BAIFA- M diet were higher than those from WFM and MP diets. This economic evaluation study clearly indicated that MP diet should be replaced by the commerical formulated EP diets as soon as possible in the near future because MP diet is not economical in the practical sea cage culture system.