• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.6135-6140
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• 2013

Background: Breast cancer is a common malignant tumor which affects health of women and multidrug resistance (MDR) is one of the main factors leading to failure of chemotherapy. This study was conducted to establish paclitaxel-resistant breast cancer cell line and nude mice models to explore underlying mechanisms of MDR. Methods: The breast cancer drug-sensitive cell line MCF-7 (MCF-7/S) was exposed in stepwise escalating paclitaxel (TAX) to induce a resistant cell line MCF-7/TAX. Cell sensitivity to drugs and growth curves were measured by MTT assay. Changes of cell morphology and ultrastructure were examined by optical and electron microscopy. The cell cycle distribution was determined by flow cytometry. Furthermore, expression of proteins related to breast cancer occurrence and MDR was tested by immunocytochemistry. In Vivo, nude mice were injected with MCF-7/S and MCF-7/TAX cells and weights and tumor sizes were observed after paclitaxel treatment. In addition, proteins involved breast cancer and MDR were detected by immunohistochemistry. Results: Compared to MCF-7/S, MCF-7/TAX cells had a higher resistance to paclitaxel, cross-resistance and prolonged doubling time. Moreover, MCF-7/TAX showed obvious alterations of ultrastructure. Estrogen receptor (ER) expression was low in drug resistant cells and tumors while expression of human epidermal growth factor receptor 2 (HER2) and Ki-67 was up-regulated. P-glycoprotein (P-gp), lung resistance-related protein (LRP) and glutathione-S-transferase-${\pi}$ (GST-${\pi}$) involved in the MDR phenotype of resistant cells and tumors were all overexpressed. Conclusion: The underlying MDR mechanism of breast cancer may involve increased expression of P-gp, LRP and GST-${\pi}$.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.21
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• pp.9265-9270
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• 2014

Background: The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions. Materials and Methods: Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA-34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a. Results: After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05). Conclusions: miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2915-2921
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• 2015

Background: Some recent clinical trials have been conducted to evaluate a combination of EGFR- TKI with chemotherapy for advanced NSCLC patients as second-line therapy, but the results on the efficacy of such trials are inconsistent. The aim of this meta-analysis was to evaluate the efficacy and safety of combination of EGFR-TKI and chemotherapy for patients with advanced NSCLC who failed first-line treatment. Materials and Methods: We searched relative trials from PubMed, EMBASE, ASCO Abstracts, ESMO Abstracts, Cochrane Library and Clinical Trials.gov. Outcomes analyzed were overall response rate (ORR), progression- free survival (PFS), overall survival (OS) and major toxicity. Results: Seven trails eventually were included in this meta-analysis, covering 1,168 patients. The results showed that the combined regimen arm had a significant higher ORR (RR 1.76 [1.16, 2.66], p=0.007) and longer PFS (HR 0.75 [0.66-0.85], p<0.00001), but failed to show effects on OS (HR 0.88 [0.68-1.15], p=0.36). In terms of subgroup results, continuation of EGFR-TKI in addition to chemotherapy after first-line EGFR-TKI resistance confered no improvement in ORR (RR 0.95 [0.68, 1.33], p=0.75) and PFS (HR 0.89[0.69, 1.15], p=0.38), and OS was even shorter (HR1.52 [1.05-2.21], p=0.03). However, combination therapy with EGFR-TKI and chemotherapy after failure of first-line chemotherapy significantly improved the ORR (RR 2.06 [1.42, 2.99], p=0.0002), PFS (HR 0.71 [0.61, 0.82], p<0.00001) and OS (HR 0.74 [0.62-0.88], p=0.0008), clinical benefit being restricted to combining EGFR-TKI with pemetrexed, but not docetaxel. Grade 3-4 toxicity was found at significantly higher incidence in the combined regimen arm. Conclusions: Continuation of EGFR-TKI in addition to chemotherapy after first-line EGFR-TKI resistance should be avoided. Combination therapy of EGFR-TKI and pemetrexed for advanced NSCLC should be further investigated for prognostic and predictive factors to find the group with the highest benefit of the combination strategy.

• Proceedings of the ESK Conference
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• 1995.10a
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• pp.41-45
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• 1995

Menu-driven interfaces are frequently employed for user -system interfaces on many electronic products. Due to the space and budget constraint, a single or multi-line display is used to show menu items. Single or Multi-line display present 8 .approx. 21 characters on an LCD screen and users select items using a series of button pushes. Multi-line displays are different from the single-line ones in the following aspects. First, they can present multiple menu items at the same time. Second, they can present menu items in a various way, for example, same-depth presentation, sub-depth presentation, previous selection, etc. In this study, a human factors experiment is being conducted to examine the effects of three independent variables on the design of a multi-line display. Factors investigated include menu structure, number of lines on the display, item presentation methodl. Usability of the multi-line display is being measured quantitatively in terms of four different aspects: task completion time, accuracy, inefficiency, user preference. A set of design guidelines will be drawn from this study which can be applied to usef-system interfaces of a various types of consumer products.

• Journal of the Korean Society of Clothing and Textiles
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• v.24 no.8
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• pp.1177-1186
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• 2000

The Study was consist of clothing shape(H-line, A-line, V-line, X-line, O-line) and color(Blace, Grey1, Grey2, White). The main purpose of this study is to analyze image differences due to shape variation, image differences due to observers characteristic differences. The main findings can be summarized as follow. First, image differences due to shape variation are statistically significant ar five factors - the maturity level, the degree of softness, attractiveness, the degree of recognition, and the activity. But the degree of recognition is found out to be the least significant factor. H-line is estimated the mature image, A·X-line is estimated young, feminine and active image. Second, image differences by observers characteristics are various depending on the shape of clothing.

• Journal of Korean Institute of Industrial Engineers
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• v.27 no.1
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• pp.95-102
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• 2001

The design of automobile body assembly line is one of essential parts for improving the process performance. The major objective of this research is to develop a performance evaluation model for automobile assembly line using a closed queueing network(CQN) and simulation method. In this study we used a two-step approach to compute the performance of the assembly line : first, we used CQN model considering assembly line equipments and the line operating time under the assumption of no failure, and second we used a well-known simulator FACTOR/AIM. Finally we implemented this model on a K automobile company and we have shown the sample results of automobile body assembly line.

• Journal of the Korean Institute of Telematics and Electronics
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• v.24 no.6
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• pp.1013-1019
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• 1987

This paper proposes the four directional line segmentation method to transform the size of dot patterns such as character dot patterns, line drawings and so on, while maintaining the quality of original pattern. At the first, this method extracts three kinds of line segmentm, namely horizontal, vertical line and slant line, and then transforms dot patterns using the each rules which are appricable to each kind of extracted line segments. Our experimental results show that transformation of dot patterns can be succeed in good quality without implementing some kinds of smoothing processes, covering the continuous transforming ratio of 0.5 to N times.

• Smart Media Journal
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• v.4 no.3
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• pp.44-49
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• 2015

Text line segmentation is a preprocessing step in OCR, which can significantly influence the accuracy of document analysis applications. This paper proposes a novel methodology for the text line segmentation of handwritten documents. First, the average width of the connected components is used to form a 1-D Gaussian kernel and a smoothing operation is then applied to the input binary image. The adaptive binarization of the smoothed image forms the final text lines. In this work, the segmentation method involves two stages: firstly, the large connected components are labelled as a unique text line using text line area mapping. Secondly, the final refinement of the segmentation is performed using the Euclidean distance between the text line and small connected components. The group of uniquely labelled text candidates achieves promising segmentation results. The proposed approach works well on Korean and English language handwritten documents captured using a camera.

• Animal Systematics, Evolution and Diversity
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• v.34 no.2
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• pp.96-100
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• 2018

One species of Euteliidae, Targalla delatrix ($Guen{\acute{e}}e$) and two species of Nolidae, Nola miyanoi Sasaki and Nola semiconfusa (Inoue) are reported for the first time in Korea. Targalla delatrix can be distinguished by the dark brownish forewings that show the transverse antemedial line, the costally rounded postmedial line and the bowl shaped marking on the costal part of subtermen and the dark brownish hindwings. Nola miyanoi can be distinguished by the whitish forewings that have the band-shaped central fascia with the blackish undulating antemedial line and the dark brownish undulating postmedial line and the whitish hindwings with a light blackish discal dot. Nola semiconfusa can be distinguished by the light grayish forewings that show deeply indented antemedial line, medially strongly projected and dentate postmedial line, a dark brownish subterminal line and a dark brownish termen, and the whitish hindwings with a light grayish discal dot. Adults and genitalia are illustrated and briefly described with their biological and distributional notes.

• Tuberculosis and Respiratory Diseases
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• v.83 no.1
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• pp.20-30
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• 2020

Tuberculosis (TB) remains a threat to public health and is the leading cause of death globally. Isoniazid (INH) is an important first-line agent for the treatment of TB considering its early bactericidal activity. Resistance to INH is now the most common type of resistance. Resistance to INH reduces the probability of treatment success and increases the risk of acquiring resistance to other first-line drugs such as rifampicin (RIF), thereby increasing the risk of multidrug-resistant-TB. Studies in the 1970s and 1980s showed high success rates for INH-resistant TB cases receiving regimens comprised of first-line drugs. However, recent data have indicated that INH-resistant TB patients treated with only firs-tline drugs have poor outcomes. Fortunately, based on recent systematic meta-analyses, the World Health Organization published consolidated guidelines on drug-resistant TB in 2019. Their key recommendations are treatment with RIF-ethambutol (EMB)-pyrazinamide (PZA)-levofloxacin (LFX) for 6 months and no addition of injectable agents to the treatment regimen. The guidelines also emphasize the importance of excluding resistance to RIF before starting RIF-EMB-PZA-LFX regimen. Additionally, when the diagnosis of INH-resistant TB is confirmed long after starting the first-line TB treatment, the clinician must decide whether to start a 6-month course of RIF-EMB-PZA-LFX based on the patient's condition. However, these recommendations are based on observational studies, not randomized controlled trials, and are thus conditional and based on low certainty of the effect estimates. Therefore, further work is needed to optimize the treatment of INH-resistant TB.