• 한국어병학회지
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• 제36권2호
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• pp.395-401
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• 2023

Myxosporeans are widespread cnidarian endoparasites in marine and freshwater ecosystems and several species were reported to be a threat to cultured fish causing serious diseases with mass mortality. In the present study, we found a myxosporean species in the genus Thelohanellus from the gills of the cultured common carp (Cyprinus carpio) fingerling for the first time in Korea. The morphological observation showed 500 ㎛ ~ 1 mm size, oval to circular shaped plasmodia containing spores which are pyriform at the anterior end and round at the posterior end (average size 20.1 ㎛ × 9.1 ㎛), with 5 to 6 turns of a single polar filament located in the polar capsule with an average size of 10 ㎛ × 4.6 ㎛. The 18S rRNA sequence was closest to the sequence of T. wangi among Thelohanellus species infecting gills but was not completely identical. Based on the morphological characteristics and molecular analysis results, we classified the present myxosporean parasite as Thelohanellus sp., temporarily. The prevalence and infection intensity of Thelohanellus sp. in the common carp fingerling were very high, which was thought to be the main cause of high mortality.

• 한국의류산업학회지
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• 제22권6호
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• pp.834-843
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• 2020

This study develops 1/2 dress forms for draping. This study investigated the production status of domestic and foreign products in order to model their shape using CLO 3D. In addition, it developed torso-type and torso-crotch-type products that allowed for draping using a 3D printer. This study analyzed shape and size compared to developed ones after referring to the Size Korea 7th Survey data as well as seven domestic and foreign company websites. The results are follows. First, an investigation of the production status of the products for the size of most of the domestic ones (except K-4) indicated that the waist circumference was slimmer than Size Korea. Bust, waist, and hip circumferences ranged from 42.0 cm, 32.0 cm, and 45.0 cm - 49.0 cm, respectively, in Chinese products; 42.0 cm - 43.0 cm, 30.5 cm - 31.5 cm, and 46.0 cm - 46.5 cm in Japanese products; 43.0 cm, 35.0cm, and 46.5 cm in American products (Japanese body shape applied). Second, an avatar was produced on a 3D software with the average size of 20-24year-old women, and its file modified as a dress form for 3D printing with PLA filament to produce dress forms for draping, workable with pins, using quilting cotton and cotton cloth. Third, the comparisons of the form and flattening between the developed dress forms and the selling ones indicated that flattening was bigger in the waist part of the former than the latter. The waist is produced too slim in the existing dress forms; therefore, it is necessary to correct the waist circumference. Unlike existing dress forms, the developed ones were produced in proximity to the average size of 20-24 year-old women in the Size Korea 7th Survey.

• 분석과학
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• 제34권2호
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• pp.87-98
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• 2021

In this study, we developed the nanoparticle multi-generator by 3D printer fusion deposition modeling (FDM) method that can reliably generate and deliver nanoparticles at a constant concentration for inhalation risk assessment. A white ABS filament was used as the test material, and SMPS was used for concentration analysis such as particle size and particle distribution. In the case of particle size, the particle size was divided by 100 nm or less and 100 to 1,000 nm, and the number of particles concentration, mass concentration, median diameter of particles, geometric average particle diameter, etc were measured. The occurrence conditions were the extruder temperature, the extruding speed of the nozzle, and the air flow rate, and experiments were conducted according to the change of conditions including the manufacturer's standard conditions. In addition, the utility of inhalation risk assessment was reviewed through a stability maintenance experiment for 6 h. As a result of the experiment, the size of the nanoparticles increased as the discharger temperature increased, as the discharge speed of the nozzle increased, and as the air flow rate decreased. Also, a constant pattern was shown according to the conditions. Even when particles were generated for a long time (6 h), the concentration was kept constant without significant deviation. The distribution of the particles was approximately 80 % for particles of 60 nm to 260 nm, 1.7 % for 1 ㎛ or larger, 0.908 mg/㎥ for the mass concentration, 111 nm for MMAD and 2.10 for GSD. Most of the ABS particles were circular with a size of less than 10 nm, and these circular particles were aggregated to form a cluster of grape with a size of several tens to several hundred nm.

• 대한토목학회논문집
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• 제29권5A호
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• pp.519-529
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• 2009

복합소재는 화학적, 역학적인 면에서 여러 장점을 가지고 있다. 피로 저항성과 화학적 저항성이 높을 뿐 아니라, 비강도, 비강성 등이 높아서 높은 감쇠 특성을 보인다. 항만 구조물에 사용되는 파일은 압축 뿐만 아니라 휨을 받기도 하므로 이에 대한 연구가 필요하다. 본 연구는 대구경을 포함하는 콘크리트 충전 유리섬유 복합소재 파일의 압축 거동 혹은 휨-압축 거동을 분석한다. 지름 및 길이가 서로 다른 25개의 실험 파일을 제작하는데, 시편의 복합소재 튜브 내경은 165 mm에서 600 mm에 이르고, 길이는 1,350 mm에서 8,000 mm에 이른다. 수적층 및 필라멘트 와인딩 성형 공법을 모두 사용하여 튜브를 제작하여 적층의 구조가 미치는 차이를 알아보았다. 충전 콘크리트의 강도로는 27 MPa과 40 MPa를 사용하였다. 축방향 및 원주방향의 섬유의 부피비에 변화를 주어 각각의 영향을 분석하였고, 일부 시편에는 나선형 홈을 튜브 안쪽에 성형하여 충전 콘크리트와 튜브 사이의 전단변형을 줄이는데 기여할 수 있는지 분석하였다. 실험결과를 보면, 직포만을 사용하여 수적층 성형 공법으로 파일을 제작하는 것보다 필라멘트 와인딩 성형 공법을 이용하여 제작하는 것이 휨강성을 높이는데 훨씬 유리하다. 나선형 홈을 성형해서 넣더라고 휨강성은 낮은 하중단계에서 부터 지속적으로 감소하는 경향을 보이는 것으로 보아, 충전 콘크리트와 튜브 사이의 전단변형을 완전히 억제하지는 못하는 것으로 판단된다.

• 미생물학회지
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• 제40권2호
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• pp.115-120
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• 2004

시안세균(Anabaena cylindrica)의 생장을 효과적으로 억제하는 세균을 분리하여 NG-2로 구분하였다. 이 세균은 그람 음성의 막대형 세균으로서 1.3∼$1.8{\times}0.35{\mu}m$ 정도의 크기를 보였으며, 카탈라제와 옥시다제 양성 반응을 나타내었고, 35∼$40^{\circ}C$아 pH 9.0의 적정생장 조건을 보였다. A.cylindrica의 NG-2를 JM 배지에서 혼합배양할 경우 두 미생물군은 서로 반비례적으로 성장하였으며, 24시간 이내에 A. cylindrica의 영양세포가 거의 완전히 소멸하였다. NG-2는 빛이 있는 조건에서만 효과적으로 A. cylindrica를 분해하였는데, 이는 NG-2의 A. cylindrica 분해활성이 숙주의 광합성 작용에 매우 의존적임을 의미한다. 분리세균의 A. cylindlica 분해작용을 현미경으로 관찰한 결과 NG-2는 숙주세포에 부착하지 않고 배지 내에 산재한 상태에서 영양세포를 분해하였으며, 이형세포는 분해되지 않았다. 또한 영양세포의 세포벽이 분해되면 분해세균들이 filament 주위에 집중적으로 부착하는 콜로니를 형성함으로써 A. cylindrica를 완전히 분해하는 것이 확인되었다. A. cylindrica에 대한 NG-2의 분해활성은 인공배지 뿐만 아니라 강물에서도 유사한 결과를 나타내었다. 또한 발포 polystyrene 재질의 bead에 세균을 부착함으로써 A. cylindrica 분해 효율을 더 상승시킬 수 있었다.

• Corrosion Science and Technology
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• 제6권6호
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• pp.282-285
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• 2007

Smaller size and higher integration of electronic systems make narrower interconnect pitch not only in chip-level but also in package-level. Moreover electronic systems are required to operate in harsher conditions, that is, higher current / voltage, elevated temperature / humidity, and complex chemical contaminants. Under these severe circumstances, electronic components respond to applied voltages by electrochemically ionization of metals and conducting filament forms between anode and cathode across a nonmetallic medium. This phenomenon is called as the electrochemical migration. Many kinds of metal (Cu, Ag, SnPb, Sn etc) using in electronic packages are failed by ECM. Eutectic SnPb which is used in various electronic packaging structures, that is, printed circuit boards, plastic-encapsulated packages, organic display panels, and tape chip carriers, chip-on-films etc. And the material for soldering (eutectic SnPb) using in electronic package easily makes insulation failure by ECM. In real PCB system, not only metals but also many chemical species are included. And these chemical species act as resources of contamination. Model test systems were developed to characterize the migration phenomena without contamination effect. The serpentine-shape pattern was developed for analyzing relationship of applied voltage bias and failure lifetime by the temperature / humidity biased(THB) test.

• Composites Research
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• 제23권3호
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• pp.37-42
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• 2010

섬유 방향 강성과 강도는 복합재 압력 용기의 성능과 밀접한 관계를 갖기 때문에, 압력 용기 구조 설계시에 다른 물성들보다 중요 설계 인자가 된다. 즉 복합재 압력 용기의 내압 변형 및 파열 압력은 섬유 물성에 의해 큰 영향을 받는다. 그러므로 정확한 섬유 방향 물성을 측정할 수 있는 기법을 확립하는 것이 복합재 압력 용기 설계 전에 우선되어야 한다. 그러나 복합재 압력용기의 섬유 방향 물성은 제작 공정 변수(와인딩 장비, 작업자, 작업환경 등)와 크기 효과에 의해 큰 영향을 받으므로 기존의 시편 시험 방법으로는 정확한 섬유 방향 물성 측정이 어렵다. 섬유 물성을 측정하는 가장 이상적인 시험 방법은 실물 압력용기 파괴시험이지만 많은 비용이 소요되어, 제품으로부터 다량의 링 시편을 채취, 내압 시험을 할 수 있는 Hoop ring 시험 방법이 제시되었다. Hoop ring 시험과 실물 압력용기의 수압 파괴 시험으로부터 구한 섬유 방향 물성들은 근접된 좋은 일치를 나타내었다.

• 대한의생명과학회지
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• 제7권4호
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• pp.167-172
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• 2001

Nebulin is an approximately 700 kDa filamentous protein in vertebrate skeletal muscle. It binds to the Z line and also binds side-by-side to the entire thin actin filament in a sarcomere. The correlation of nebulin size with thin filament length have led to the suggestion that nebulin acts as a molecular ruler for the length of thin filaments. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain. SH3 domains have been identified in an ever-increasing number of proteins important for a wide range of cellular processes, from signal transduction to cytoskeleton assembly and membrane localization. However, the exact physiological role of SH3 domains remains, in many cases, unclear. To explore the role of nebulin SH3 in the cytoskeletal rearrangement that accompanies myoblast differentiation, we transfected sense and antisense nebulin SH3 domain fused to enhanced green fluorescent protein in myoblast. Cells expressing nebulin SH3 fragment showed decrease of cell-cell adhesion, and cells transfected with antisense nebulin SH3 gene showed a rounded cell morphology and loss of cell-matrix adhesion. No alteration in cell shape and differentiation were observed in control cells expressing enhanced green fluorescent protein. Perturbation of nebulin altered the cell shape and disrupted cell adhesion in myoblast, demonstrating that nebulin can affect cytoskeleton rearrangement.

• 대한약침학회지
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• 제23권1호
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• pp.30-36
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• 2020

Objectives: The objective of the study was to prepare Bauhinia variegata loaded nanoemulsion(formulation and determine the efficacy of herbal drug formulation against diabetic peripheral neuropathic pain through acupuncture technique. Methods: Nine different ba tches of nanoemulsion (NE1 NE9) of BVN was prepared by varying the S_mix ratio and the concentration of oil. BVN was characterized to determine particle size, shape, zeta potential, polydispersity index, optical transmittance, drug release profile and stora ge stability. The optimized formulation was subjected to plantar test, behavioral tests of neuropathic pain and Von Frey filament stimulation test. Diabetes was induced by intraperitoneal injection of freshly prepared solution of Streptozotocin (60 mg/kg) to the experimental rats. Animals were made diabetic divided into four groups, Group I was untreated normal control group, Group II was diabetic control group, Group III was Bauhinia variegata extract ( treated group (100 mg/kg/day, p.o) and Group IV was BVN treated groups (100 mg/kg/day, p.o) acute and chronically. Results: The prepared B. variegata loaded nanoemulsion was nanosized (124 nm), spherical, uniform and stable over the period of 180 days with no change in physiochemical properties. The bl ood glucose and body weight of animals was normalizing after four weeks of treatment that was significant with BVN in comparison to diabetic control group. The chronic administration of BVN significantly (P<0.001) decreased hind paw withdrawal latency an d attenuated mechanical allodynia as compared with diabetic rats. Conclusion: Thus, BVN may be an effective drug formulation against diabetic peripheral neuropathic pain.

• 한국농공학회논문집
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• 제57권6호
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• pp.141-152
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• 2015

Most of agricultural structures are consisted of complex components and exposed to various boundary conditions. There have been no ways to express those structures exactly for model experiment. As an alternative, 3D printer can produce any type of solid model. However, there are limited informations related to structural experiments using 3D printer. The object of this study gives the basic informations to structural engineers who try to use 3D printer for model experiment. When PLA was used as a supplier for 3D printer, the outcomes showed less heat deformation to compare with ABS. To test the material properties, two kinds of experiments (three-point flexibility test and compression test) were executed using universal testing machine. In three-point flexibility test, plastic hinge and its deformation were developed as observed in material such as steel. The behavior was in a linear elastic state, and elastic bending modulus and yield force were evaluated. In the compression test using unbraced columns with hinge-hinge boundary condition, the constant yield forces were observed regardless of different lengths in all columns with same section size, whereas the compressive elastic modulus was increased as the length of column was increased. The suggested results can be used for model experiments of various agricultural structures consisted of single material.