• Title/Summary/Keyword: Field evaluation

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Evaluation of Agronomic Characteristics, Nutritional Contents, and Insect Response of the Transgenic Potato Resistant to Glufosinate Ammonium (제초제 저항성 형질전환 감자의 농업적 특성, 영양 성분 및 해충 반응성 평가)

  • Ahn, Soon-Young;Cho, Kwang-Soo;Seo, Hyeo-Won;Yi, Jeong-Yoon;Bae, Shin-Cheol;Cho, Ji-Hong;Park, Young-Eun;Kim, Ju-Il;Kim, Hyun-Jun;Cho, Hyun-Mook
    • Horticultural Science & Technology
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    • v.29 no.3
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    • pp.247-254
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    • 2011
  • The agronomic characteristics, nutritional contents, and insect response of the potato clones transformed with a glufosinate ammonium resistance gene were evaluated. Among the 4 transgenic potato clones, the Bar 3 clone was selected as a promising one for commercialization. The Bar 3 clone showed similar tuber yield capacity but higher herbicide resistance as compared with the non-transgenic potato cv. Dejima. The herbicide resistance of the Bar 3 clone was more than 5 times higher when tested with the herbicide concentration recommended by the producer. The major agronomic characteristics of the Bar 3 clone were not different from those of the non-transgenic Dejima. The annual variation in yields and agronomic characteristics showed similar tendency for 2 years from the third to fourth generation after transformation. The tubers of the Bar 3 clone also showed low occurrence in common scab and physiological disorders such as cracking and secondary growth. But the reasons for such results are yet to be studied. Also, it was considered that the Bar 3 clone have a potential of reducing not only common scab occurrence but also soil erosion during potato cultivation in field. The nutritional contents (mineral compound, vitamin C and amino acid) and response to Spodoptera exigua of the transgenic potato clones were not significantly different.

Evaluation of the Efficiency of E. coli O157: H7 Rapid Detection Kit using Immunochromatography (면역크로마토그래피를 이용한 E. coli O157: H7 신속검출 키트의 유효성 평가)

  • Kwak, Hyo-Sun;Lee, Dong-Ha;Moon, Hee-Sook;Park, Jong-Seok;Woo, Gun-Jo;Kim, Chang-Min
    • Journal of Food Hygiene and Safety
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    • v.18 no.3
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    • pp.118-124
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    • 2003
  • For the rapid detection of various pathogenic microorganisms from food sample, various kinds of kits have been developed and commercially available in the markets. With the advantages of speed, accuracy and easiness, the market of these kits has gradually increased for the QC and QA field of food company as well as testing facilities or laboratories. In this study, the characteristics such as the detection limit and the sensitivity of immunochromatographic type of rapid detection kit (Donga Co, Korea, D-kit) for E. coli 0157:H7 developed by monoclonal antibody were examined and also the possibility of application of the kit to food samples was evaluated. The reference kits used for comparison study were Reveal E. coli 0157:H7 (Neogen Co., USA, R-kit) and VIP EHEC kit (Biocontrol Inc., USA, V-kit) occupying major market share. In the detection limit test with the E. coli 0157:H7 reference, both R-kit and D-kit showed a distinct positive reaction in $10^4$/ml and weak positive reaction in $10^3$/ml, whereas V-kit showed a same reaction in 105/ml. Also, it was identified that the culture treated with heat showed more sensitivity than no heat treated culture. The sensitivity test was conducted against 22 isolates of E. coli 0157:H7, 7 strains of non-O157:H7 verotoxin-producing E. coli, 40 strains of E. coli with different O and H antigen type, and 38 strains of non-E. coli Enterobacteriaceae, and all of the test strains except three were showed exactly three were showed exactly the same reaction against three kinds of the tested kits. All the three kinds of kits showed a positive reaction against E. coli O157:H19, E. coli O148:H18 and Salmonella galinarium. We suppose that there might be a similarity in serological property between these three strains and O157:H7. From the test results, it can be concluded that there is (was) no difference between the D-kit developed in this study and R-kit or V-kit based on the detection limit and sensitivity.

Effects of Milk Production, Postparient Days or Seasons on In Vivo Embryo Production by Superovulation in Holstein Cows (유우의 과배란 처리에 있어서 산유량, 분만 후 처리시기 및 계절이 체내수정란 생산에 미치는 영향)

  • Lim, Kwang-Taek
    • Journal of Embryo Transfer
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    • v.24 no.1
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    • pp.33-37
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    • 2009
  • Multiple ovulation and embryo transfer (MOET) has the potential to increase the rates of genetic improvement in cattle. Thus this study was performed to investigate several factors influencing in vivo embryo production in Holstein cattle under field conditions. The donors were superovulated with Folltropin-V and $PGF_2{\alpha}$ combination method. From Day 10 onward, donors were superovulated by i.m., twice daily, administration of 400mg Folltropin-V given in a series of decreasing doses over a 4-day period: on the first day, 3.5ml; on the second day, 3.0ml; on the third day, 2.0ml; and on the fourth day, 1.5ml (20ml in total, equivalent to 400mg of NIH-FSH-P1). Estrus was induced by i.m. administration of 25mg prostaglandin $F_2{\alpha}$ on the sixth and seventh of FSH treatment. Estrus detection was performed twice daily beginning 24h after the first prostaglandin $F_2{\alpha}$ injection. Donor cows were artificially inseminated 12 and 24 h after first standing estrus with semen from a proven Holstein sire. Embryos used in this study were recovered Day 7.5 of the cycle (Day 0: first standing estrus). From 195 superovulated dairy cows, 2,104 eggs were recovered, of which 1,172 were classified as transferable embryos based on morphological evaluation of quality. The results are summarized as follows: 1. The numbers of recovered and transferable embryos did not significantly differ among the capacity of milk production that were < 10,000kg/305days (group 1), $10,000{\sim}12,000\;kg$/305days (group 2) or > 12,000kg/305 days (group 3) (p>0.05, Table 1). 2. No differences in the numbers of recovered and transferable embryos were found among the donor's postparient days (p>0.05, Table 2). 3. Also, the numbers of recovered and transferable embryos of each superovulation seasons did not significantly differ among the four groups (p>0.05, Table 3).

Evaluation of shear-bond strength between different self-adhesive resin cements with phosphate monomer and zirconia ceramic before and after thermocycling (인산염계 기능성 단량체가 첨가된 수종의 자가 접착 레진시멘트와 지르코니아 세라믹 사이 열순환 전후 전단결합강도 비교)

  • Lee, Ji-Hun;Kim, Min-Kyung;Lee, Jung-Jin;Ahn, Seung-Geun;Park, Ju-Mi;Seo, Jae-Min
    • The Journal of Korean Academy of Prosthodontics
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    • v.53 no.4
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    • pp.318-324
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    • 2015
  • Purpose: This study compared shear bond strengths of five self-adhesive cements with phosphate monomer to zirconium oxide ceramic with and without airborn particle abrasion. Materials and methods: One hundred zirconia samples were air-abraded ($50{\mu}mAl_2O_3$). One hundred composite resin cylinders were fabricated. Composite cylinders were bonded to the zirconia samples with either Permacem 2.0 (P), $Clearfil^{TM}$ SA Luting (C), $Multilink^{(R)}$ Speed (M), $RelyX^{TM}$ U200 Automix (R), G-Cem $LinkAce^{TM}$ (G). All bonded specimens were stored in distilled water ($37^{\circ}C$) for 24 h and half of them were additionally aged by thermocycling ($5^{\circ}C$, $55^{\circ}C$, 5,000 times). The bonded specimens were loaded in shear force until fracture (1 mm/min) by using Universal Testing Machine (Model 4201, Instron Co, Canton, MA, USA). The failure sites were inspected under field-emission scanning electron microscopy. The data was analyzed with ANOVA, Tukey HSD post-hoc test and paired samples t-test ($\alpha$=.05). Results: Before and after thermocycling, $Multilink^{(R)}$ Speed (M) revealed higher shear-bond strength than the other cements. G-Cem $LinkAce^{TM}$ (G) showed significantly lower bond strengths after thermocycling than before treatment (P<.05), but the other groups were not significantly different (P>.05). Conclusion: Most self-adhesive cements with phosphate monomer showed high shear bond strength with zirconia ceramic and weren't influenced by thermocycling, so they seem to valuable to zirconia ceramic bonding.

A Study on the ICAO international aviation safety policy, a change of paradigm and the government response to the direction (ICAO 국제항공안전정책 패러다임의 변화 분석과 우리나라 신국제항공안전정책 검토)

  • Chang, Man-Heui;Hwang, Ho-Won
    • The Korean Journal of Air & Space Law and Policy
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    • v.28 no.1
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    • pp.73-96
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    • 2013
  • ICAO's Universal Safety Oversight Audit Programme (USOAP) was initially launched in January 1995, in response to widespread concerns about the adequacy of aviation safety oversight around the world. The recent reduction in aircraft accidents and effective role that is evaluated on the basis of these results, and in 2013 the existing 'snapshot approach' to 'regular monitoring system (USOAP-Continuous Monitoring Approach)' was converted to. ICAO aviation safety assessment of the state in today's international community 'aviation safety credibility' as objective indicators to judge the enormous impact on the aviation industry, the state is not satisfactory, especially if the results of the evaluation and expansion of code-share airline ban, reduced international air transit passengers, including premium increases business and economic penalties should. In addition, ICAO implementation of the existing laws and regulations(Prescriptive Approach), but based on the Risk-based prevention model, Proactive Approach introduced the concept of aviation safety system, including international aviation safety policy has been to switch paradigms. This new ICAO international aviation safety policy also applies to the Government of the Republic of Korea in line with the aviation safey policies have changed. In particular, the systematic implementation of safety management for the existing laws and regulations in the center of the safety oversight system of risk-based introduction of the concept of proactive safety management, and According to international standards ICAO aviation service providers operate their own Safety Management System was set out in Aviation Law ever. In addition, the aviation safety is at the center of the field of the safety of aircraft operations and maintenance for the promotion is promoting various safety policies. This new paradigm shift in the international aviation safety policy in line with our state in the international community with the most exemplary aviation safety system firmly established itself as a model, the Government will strengthen the competitiveness of our aviation plans to support. To do this, the government, airlines, aviation officials try all the practical effect would be expected.

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Evaluation of Fertilization Effect of Slow-Release Complex Fertilizer on Pepper Cultivation (원예용 완효성 복합비료의 고추에 대한 시비효과 평가)

  • Lee, Chang-Hoon;Lee, Hyub;Ha, Byung-Hyun;Kang, Chang-Sun;Lee, Yong-Bok;Kim, Pil-Joo
    • Korean Journal of Environmental Agriculture
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    • v.26 no.3
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    • pp.228-232
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    • 2007
  • Slow-release fertilizers (SRF) have been used to reduce nutrient loss through increasing fertilizer efficiency and to save labor. Several SRFs were developed for rice plant in Korea, but there is few for horticultural crop plants. Two slow-release complex fertilizers, 100T and 150T, which made for controlling nitrogen release time up to 100 and 150 days, respectively, were selected for the incubation test cto evaluate nitrogen (N) release rate in soil. The N of urea selected as the control was completely released within a week after application. Sixty three and 53% of total N were released from 110T and 150T of slow release fertilizers within 8th weeks after application, respectively. For pepper cultivation CF110 and CF150, new slow-release complex fertilizer, were made of mixing 40% of conventional fertilizer and 60% of 110T and 150T, respectively, based on the amount of recommended fertilizer for pepper cultivation $(N-P_2O_5-K_2O=190-112-149\;kg\;ha^{-1})$, and were totally applied before pepper transplanting in the field as the basal fertilizer. Inorganic N $(NH_4^+-N+NO_3^--N)$ concentration in soil was higher in the CF110 treatment than in the control (NPK) at all period of pepper cultivation. In the CF150 treatment concentration of inorganic N in soil was low compared to control up to 8th weeks after transplanting. However, there was no difference in plant height and nutrient content of pepper leave between CF110 treatment and the control. In comparison, plant height was significantly lower in CF150 than the control and CF110 treatments. Around 4% of fresh pepper yield was increased in CF110 compared to the control, but it was decreased to about 2% by CF150 treatment. Conclusively, CF110 form could be recommended as a slow release fertilizer for pepper cultivation.

Change in the Korean Fertility Control Policy and its Effect (출산력 억제정책의 영향과 변천에 관한 고찰)

  • Hong, Moon-Sik
    • Korea journal of population studies
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    • v.21 no.2
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    • pp.182-227
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    • 1998
  • Korean government decided to adopt an explicit population control policy in 1961 and from the following year the establishment and operation of the national family planning programme was included in each of the Five-Year Economic Development Plans that began in 1962. This policy was pursued in the understanding that without proper population control measures korea could not be able to achieve economic development. Korean national family planning programme is characterized by contraceptive target system through public health network with distribution of free contraceptives by family planning field workers and clinical contraceptive services such as IUD, vasectomy and tubaligation at designated clinics by the government. In addition, IE&C activities by the Planned Parenthood Federation of Korea and programme evaluation and research by the Korea Institute for Health and Social Affairs contributed to the development of the government programme. Between 1960 and mid 1980s the nation's total fertility rate was reduced from 6.0 to population replacement level of 2.1 and thereafter it has been maintained at 1.6 to 1.7 of below replacement level. With a short period of less than three decades fertility transition was completed in Korea. It is estimated that if the current level of below replacement fertility continues, the population in Korea stabilize at around 52.78 million people by the year 2028, and it will begin to decrease thereafter. Under this circumstances, the government adopted new directions and strategies of the population policy in June 1996, focused primarily on population quality and social welfare than on demographic arenas. In spite of over 80 percent of high contraceptive prevalence among married women, high incidence of induced abortions is maintained. Moreover, the prevalence of sex selective induced abortions using procedures to determine the sex of the fetus has resulted in an imbalance in the sex ratio at birth. In order to overcome those problems associated with reproductive health new policy for population quality control and health promotion programme should be highly strengthened in the future.

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Predicting the Goshawk's habitat area using Species Distribution Modeling: Case Study area Chungcheongbuk-do, South Korea (종분포모형을 이용한 참매의 서식지 예측 -충청북도를 대상으로-)

  • Cho, Hae-Jin;Kim, Dal-Ho;Shin, Man-Seok;Kang, Tehan;Lee, Myungwoo
    • Korean Journal of Environment and Ecology
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    • v.29 no.3
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    • pp.333-343
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    • 2015
  • This research aims at identifying the goshawk's possible and replaceable breeding ground by using the MaxEnt prediction model which has so far been insufficiently used in Korea, and providing evidence to expand possible protection areas for the goshawk's breeding for the future. The field research identified 10 goshawk's nests, and 23 appearance points confirmed during the 3rd round of environmental research were used for analysis. 4 geomorphic, 3 environmental, 7 distance, and 9 weather factors were used as model variables. The final environmental variables were selected through non-parametric verification between appearance and non-appearance coordinates identified by random sampling. The final predictive model (MaxEnt) was structured using 10 factors related to breeding ground and 7 factors related to appearance area selected by statistics verification. According to the results of the study, the factor that affected breeding point structure model the most was temperature seasonality, followed by distance from mixforest, density-class on the forest map and relief energy. The factor that affected appearance point structure model the most was temperature seasonality, followed by distance from rivers and ponds, distance from agricultural land and gradient. The nature of the goshawk's breeding environment and habit to breed inside forests were reflected in this modeling that targets breeding points. The northern central area which is about $189.5 km^2$(2.55 %) is expected to be suitable breeding ground. Large cities such as Cheongju and Chungju are located in the southern part of Chungcheongbuk-do whereas the northern part of Chungcheongbuk-do has evenly distributed forests and farmlands, which helps goshawks have a scope of influence and food source to breed. Appearance point modeling predicted an area of $3,071 km^2$(41.38 %) showing a wider ranging habitat than that of the breeding point modeling due to some limitations such as limited moving observation and non-consideration of seasonal changes. When targeting the breeding points, a specific predictive area can be deduced but it is difficult to check the points of nests and it is impossible to reflect the goshawk's behavioral area. On the other hand, when targeting appearance points, a wider ranging area can be covered but it is less accurate compared to predictive breeding point since simple movements and constant use status are not reflected. However, with these results, the goshawk's habitat can be predicted with reasonable accuracy. In particular, it is necessary to apply precise predictive breeding area data based on habitat modeling results when enforcing an environmental evaluation or establishing a development plan.

A comparative Study of Changing Pattern of Cause of Death Analysis of Korean, Korean in Japan and Japanese (재일한국인의 생활문화의 이질화와 적응과정에 관한 보건학적 연구(제 1보 한국, 재일한국인, 일본의 사인구조분석)

  • 김정근;장창곡;임달오;김무채;이주열
    • Korea journal of population studies
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    • v.15 no.2
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    • pp.15-59
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    • 1992
  • After world war II Japanese life expectancy has been improved remarkably, and reached the highest level in the world around late 1970's. The life expectancy of Korean has also shown tremendous improvement in recent years with about 20 year's gap from the Japanese. The reason of rapid improvement of life expectancy can be explained by changes in the structure of cause of death due to health system, living standard, social welfare, health behavior of individuals and so on. Korean in Japan is placed under different situations from both Korean in Korea and Japanese in these regards, and expected to show different picture of cause of death pattern. The objective of this study is the comparision of changing patterns of cause of death of three population groups, Korean in Japan, Korean in Korea and Japanese, and to investigate the reasons which effect to the structural difference of mortality cause with special emphasis on health ecological aspects. One of the major limitations of the Korean causes of death statistics is the under-registration which ranges about 10% of the total events, and inaccuracy of the exact cause of death. Some 20% of registered deaths were unable to classify by ICD. However, it is concluded that the Korean data are evaluated as sufficient to stand for over-viewing of trends of cause of death pattern. The evaluation is done by comparing data from registration and field survey over the same population sample. Population data of Korean in Japan differ between two sources of data; census and foreigner's registration. Correction is done by life table method under the assumption that age-specific mortality pattern would accord with that of the Japanese. The crude death rate was lowest among Korean in Japan, 5.7 deaths per 1,000 population in 1965. The crude death rates of Korean in Japan and Japanese are increasing recently influenced by age structure while Korean in Korea still shows decreasing tendency. The adjusted death rate is lowest among Japanese, followed by Korean in Japan, and Korean in Korea. The leading causes of death of Korean in Korea until 1960's was infectious diseases including pneumonia and tuberculosis. The causes of death structure changed gradually to accidents, neoplasm, hypertensive disease, cerebro-vascular disease in order. The main difference in cause of death between Korean and Japanese if high rate of liver diseases and diabetes for both Korean in Japan and Korea. A special feature of cause of death among Korean in Korea is remakably high rate of hypertensive disease, which is assumed to be caused by physicians tendency in choosing diagnostic categories. The low ischemic heart disease and high vasculo-cerebral disease are the distinctive characteristic of the three population groups compared to western countries. Specific causes of death were selected for detailed sex, age and ethnic group comparisons based on their high death rates. Cancer is the cause of death which showed most dramatical increase in all three population groups. In Korea 20.1% of all death were caused by cancer in 1990 compared with 10.5% in 1981. Cancer of the liver is the leading cause of cancer death among Korean in Japan for both sexes, followed by cancer of the lung and cancer of the stomach, while that of Korean in Korea is cancer of the stomach, followed by cancer of the liver and cancer of the lung for male. Causes of infant mortality were examined among the three population groups since 1980 on yearly bases. For both Japanese and Korean in Japan, leading cause of death ranks as conditions originating in the perinatal period, congenital anomalies, accidents and other violent causes. Trends since 1980 for these two population groups in the leading cause of infant mortality showed no changes. On the contrary, significant changes in leading cause of death structure in Korea were observed : the ranking of leading cause of death in 1981 were congenital asnomalies, pneumonia bronchitis, infectious disease, heart disease, conditions originating in the perinatal period, accident and other violent causes ; in 1990 the ranking shifted to congenital anomalies, accident, pneumonia bronchities, conditions originating in the perinatal period, infectious disease. The mortality rate by congenital anomalies in Korea continuously grew than any other causes. Larger increase ocurred during the 1990's

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The Influence of Adjuvants on Herbicide Activity of Streptomyces scopuliridis KR-001 (토양 방선균 Streptomyces scopuliridis KR-001 균주 배양액의 살초활성을 증가시키는 Adjuvant 탐색)

  • Kim, Jae Deok;Sin, Hoon Tak;Kim, Young Sook;Ko, Young Kwan;Cho, Nam Kyu;Hwang, Ki Hwan;Koo, Suk Jin;Choi, Jung Sup;Park, Kee Woong
    • Weed & Turfgrass Science
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    • v.4 no.4
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    • pp.288-294
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    • 2015
  • This study was conducted to investigate efficient adjuvants to increase herbicidal efficacy of metabolites from Streptpmyces scopuliridis KR-001. Commonly used 21 adjuvants mixed with the metabolites were applied to eight weed species (six grass weeds and two broadleaved weeds). Based on the visual evaluation, two adjuvants, LE7 (Polyoxyethylene lauryl ether) and EP4C (Sodium bis (2-ethylhexyl) sulfosuccinate), were selected as most efficient adjuvants to elevate herbicidal efficacy of the metabolites. Higher efficacy in the LE7 and EP4C was obtained when overall spray volume was $2,000L\;ha^{-1}(65{\mu}g\;a.i.\;ml^{-1})$ than $1,000L\;ha^{-1}(130{\mu}g\;a.i.\;ml^{-1})$. Field study demonstrated that $1,300{\mu}g\;ml^{-1}$ of metabolites from KR-001 applied with EP4C at concentration of $2{\mu}g\;ml^{-1}$ provided a highly effective post-emergence weed control which was almost equivalent to the glufosinate-ammonium at $540g\;a.i.\;ha^{-1}$. On the basis of these results, combination and multiple application methods could be developed to enhance herbicidal efficacy of metabolites from KR-001.