• Smart Structures and Systems
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• v.6 no.5_6
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• pp.423-438
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• 2010

Wireless smart sensors enable new approaches to improve structural health monitoring (SHM) practices through the use of distributed data processing. Such an approach is scalable to the large number of sensor nodes required for high-fidelity modal analysis and damage detection. While much of the technology associated with smart sensors has been available for nearly a decade, there have been limited numbers of fulls-cale implementations due to the lack of critical hardware and software elements. This research develops a flexible wireless smart sensor framework for full-scale, autonomous SHM that integrates the necessary software and hardware while addressing key implementation requirements. The Imote2 smart sensor platform is employed, providing the computation and communication resources that support demanding sensor network applications such as SHM of civil infrastructure. A multi-metric Imote2 sensor board with onboard signal processing specifically designed for SHM applications has been designed and validated. The framework software is based on a service-oriented architecture that is modular, reusable and extensible, thus allowing engineers to more readily realize the potential of smart sensor technology. Flexible network management software combines a sleep/wake cycle for enhanced power efficiency with threshold detection for triggering network wide operations such as synchronized sensing or decentralized modal analysis. The framework developed in this research has been validated on a full-scale a cable-stayed bridge in South Korea.

• Journal of the Korea Society for Simulation
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• v.27 no.1
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• pp.33-38
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• 2018

This paper introduces the simulation concepts and technical approach of wire-guided torpedo performance analysis simulator, as a consequence, provide a framework for understanding overall attack procedures and effectiveness of tactics to torpedo operator. It described the mathematical models of simulation components and weapon engagement principle, especially it derived the closed-form solution of time consumption and leading angle problem of torpedo attack situation based on geographical assumption. In addition, it adopted the proportional navigation guidance at final stage of torpedo attack and also consider the tradeoff relation between target ship speed(propeller noise level) and detection probability, so that it improves the fidelity of physical realism. Simulator is developed with high degree of freedom in the perspective of tactical situation, and it helps user to understand the overall situation and tactical effectiveness.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.3
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• pp.121-128
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• 2021

Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl₂. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 ㎛ of CdCl₂, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl₂ untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl₂ in HC11 cells decreased the mRNA expression of Rad51.

• Genomics & Informatics
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• v.18 no.4
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• pp.42.1-42.9
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• 2020

Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-Seq) is a powerful technology to profile the location of proteins of interest on a whole-genome scale. To identify the enrichment location of proteins, many programs and algorithms have been proposed. However, none of the commonly used peak calling programs could accurately explain the binding features of target proteins detected by ChIP-Seq. Here, publicly available data on 12 histone modifications, including H3K4ac/me1/me2/me3, H3K9ac/me3, H3K27ac/me3, H3K36me3, H3K56ac, and H3K79me1/me2, generated from a human embryonic stem cell line (H1), were profiled with five peak callers (CisGenome, MACS1, MACS2, PeakSeq, and SISSRs). The performance of the peak calling programs was compared in terms of reproducibility between replicates, examination of enriched regions to variable sequencing depths, the specificity-to-noise signal, and sensitivity of peak prediction. There were no major differences among peak callers when analyzing point source histone modifications. The peak calling results from histone modifications with low fidelity, such as H3K4ac, H3K56ac, and H3K79me1/me2, showed low performance in all parameters, which indicates that their peak positions might not be located accurately. Our comparative results could provide a helpful guide to choose a suitable peak calling program for specific histone modifications.

• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.157-165
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• 2019

Di- and tri-methylation of lysine 4 on histone H3 (H3K4me2 and H3K4me3, respectively) are epigenetic markers of active genes. Complex associated with Set1 (COMPASS) mediates these H3K4 methylations. The involvement of COMPASS activity in secondary metabolite (SM) biosynthesis was first demonstrated with an Aspergillus nidulans cclA knockout mutant. The cclA knockout induced the transcription of two cryptic SM biosynthetic gene clusters, leading to the production of the cognate SM. Monascus spp. are filamentous fungi that have been used for food fermentation in eastern Asia, and the pigment Monascus azaphione (MAz) is their main SM. Monascus highly produces MAz, implying that the cognate biosynthetic genes are highly active in transcription. In the present study, we examined how COMPASS activity modulates MAz biosynthesis by inactivating Monascus purpureus cclA (Mp-cclA) and swd1 (Mp-swd1). For both ${\Delta}Mp-cclA$ and ${\Delta}Mp-swd1$, a reduction in MAz production, accompanied by an abated cell growth, was observed. Suppression of MAz production was more effective in an agar culture than in the submerged liquid culture. The fidelity of the ${\Delta}Mp-swd1$ phenotypes was verified by restoring the WT-like phenotypes in a reversion recombinant mutant, namely, trpCp: Mp-swd1, that was generated from the ${\Delta}Mp-swd1$ mutant. Real-time quantitative Polymerase chain reaction analysis indicated that the transcription of MAz biosynthetic genes was repressed in the ${\Delta}Mp-swd1$ mutant. This study demonstrated that MAz biosynthesis is under the control of COMPASS activity and that the extent of this regulation is dependent on growth conditions.

• Journal of the Korea Society for Simulation
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• v.28 no.2
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• pp.89-95
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• 2019

This paper introduces the simulation concepts and technical approach of underwater weapon system performance analysis simulator, especially focused on probabilistic target detection concepts. We calculated the signal excess (SE) value using SONAR equation, then derived the probability density function(PDF) for target presence($H_1$) or absence($H_0$) cases, respectively. With the Neyman-Pearson detector criterion, we got the probability of detection($P_D$) while satisfying the given probability of false alarm($P_{FA}$). At every instance of simulation, target detection is decided in the probabilistic perspective. With the proposed detection implementation, we improved the model fidelity so that it could support the tactical decision during the operation.

• BioChip Journal
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• v.12 no.4
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• pp.332-339
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• 2018

The future neuro-prosthetic devices would be required spikes data monitoring through sub-nanoscale transistors that enables to neuroscientists and clinicals for scalable, wireless and implantable applications. This research investigates the spikes monitoring through integrated CNT front-end amplifier for neuro-prosthetic diagnosis. The proposed carbon nanotube-based architecture consists of front-end amplifier (FEA), integrate fire neuron and pseudo resistor technique that observed high electrical performance through neural activity. A pseudo resistor technique ensures large input impedance for integrated FEA by compensating the input leakage current. While carbon nanotube based FEA provides low-voltage operation with directly impacts on the power consumption and also give detector size that demonstrates fidelity of the neural signals. The observed neural activity shows amplitude of spiking in terms of action potential up to $80{\mu}V$ while local field potentials up to 40 mV by using proposed architecture. This fully integrated architecture is implemented in Analog cadence virtuoso using design kit of CNT process. The fabricated chip consumes less power consumption of $2{\mu}W$ under the supply voltage of 0.7 V. The experimental and simulated results of the integrated FEA achieves $60G{\Omega}$ of input impedance and input referred noise of $8.5nv/{\sqrt{Hz}}$ over the wide bandwidth. Moreover, measured gain of the amplifier achieves 75 dB midband from range of 1 KHz to 35 KHz. The proposed research provides refreshing neural recording data through nanotube integrated circuit and which could be beneficial for the next generation neuroscientists.

• Journal of the Korea Computer Graphics Society
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• v.25 no.3
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• pp.21-29
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• 2019

This paper proposes a novel framework for image fusion of satellite imagery to enhance spatial resolution of the image via structure-texture decomposition. The resolution of the satellite imagery depends on the sensors, for example, panchromatic images have high spatial resolution but only a single gray band whereas multi-spectral images have low spatial resolution but multiple bands. To enhance the spatial resolution of low-resolution images, such as multi-spectral or infrared images, the proposed framework combines the structures from the low-resolution image and the textures from the high-resolution image. To improve the spatial quality of structural edges, the structure image from the low-resolution image is guided filtered with the structure image from the high-resolution image as the guidance image. The combination step is performed by pixel-wise addition of the filtered structure image and the texture image. Quantitative and qualitative evaluation demonstrate the proposed method preserves spectral and spatial fidelity of input images.

• Journal of the Korea Society for Simulation
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• v.28 no.1
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• pp.41-47
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• 2019

Defense simulation is actively used to test various weapon systems and evaluate their effectiveness. The AddSIM environment is a simulation framework designed to support the weapon systems dealt with in defense simulation from an integrated point of view and is designed for reuse and scalability. Models used in AddSIM require base model structure fidelity and verification of user code area. Therefore, this paper describes the dynamic verification method used for completeness of models used in AddSIM. For the dynamic verification of user code, the specification method and the verification algorithm are described. Also, we introduce the prototype of the dynamic verifier implemented based on verification specification method and algorithm. The case study analyzes the verification results based on the simulation example implemented in AddSIM environment.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.34 no.8
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• pp.69-76
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• 2018

This study is basically about the changing appearance of the big pavilion, Yeongnamnu in Miryang city belonging to the government guesthouse of Joseon dynasty. And the purpose of this study confirms that even if the appearance of the building has not changed, the function of the building changes because the reason why people think about the building is also different. First, looked at the appearance of architecture and the function of architecture that changes with the times. In the course of the research, the changes of pavilion were traced by dividing it into five from Kory? dynasty which was first established to the present. Then, tried to find out the change patterns by analyzing important events related to buildings or places in each period. As a result, the following facts were confirmed. 1. Because of the Buddhist repression policy in Joseon Dynasty, the Yeongnamnu changed from the temple pavilion to the government pavilion. 2. Throughout the Joseon Dynasty, Yeongnamnu was the representative scenic of Miryang City. 3. In the late Joseon Dynasty, Yeongnamnu was famous as a place where a woman named Arang devoted her life in order to preserve his fidelity. 4. Since 1910, Yeongnamnu has become an important cultural asset, and space has been widely used as a venue for various public events. 5. From 1945 to 1999, Yeongnamnu was used mainly for the events of Arang Remembrance and Miryang Culture Festival. And the inner space of Yeongnamnu was rarely used because people thought preservation is important.