• 대한치과보철학회지
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• 제25권1호
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• pp.327-340
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• 1987

This study was performed to investigate the biocompatibility of several dental casting alloys (Gold, Dong Myung A-45, Dong Myung AP-35, Verabond, Rexillium, NPG) employing tissue culture. Fibroblast-like cells derived from the subcutaneous tissue of Sprague-Dawley rat were cultivated in DME medium with the addition of those alloys. Results were assessed by calculating the cell multiplication rate and relative growth rate and by observing the morphology of cells in the presence of the specimens. Gold was indicated to be most biocompatible with fibroblast-like cell. Dong Myung A-45 and Dong Myung AP-35 showed very similar effects on the cells as did Gold. Also there was a decrease in cytotoxicity of the alloys as the concentration of gold increased. Verabond and Rexillium showed a decreased in cell mutiplication rate as compared to low gold alloys. NPG exhibited the most severe cell toxicity among the tested alloys.

• 대한후두음성언어의학회지
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• 제23권1호
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• pp.28-32
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• 2012

Vocal fold scar disrupts structure of lamina propria and causes significant change in vocal fold tissue biomechanics, resulting in a range of voice problems that often significantly compromise patient quality of life. Although several therapeutic management have been offered in an attempt to improve vocal fold scar, the ideal treatment has not yet been found. Recently, several tissue engineering technique for vocal fold scar using growth factors, several cells, and scaffolds have been described in tissue culture and animal models. Several growth factors such as hepatocyte growth factor, basic fibroblast growth factor, and transforming growth factor beta 3 for therapy and prevention of vocal fold scar have been studied. Cell types to regenerate vocal folds in scarring tissue have been introduced autologous or scarred vocal fold fibroblast and adult mesenchymal stem cells. Decellularized organ matrix and several hyaluronic acid materials have used as scaffolds for vocal fold scar.

• Korean Journal of Acupuncture
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• 제28권3호
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• pp.43-51
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• 2011

Objectives : In this study, we investigated the effect of Fructus Ligustri Lucidi $H_2O$ fraction (FLLW) on cell proliferation, and the phosphorylation of ERKs and Akt in human dermal fibroblast neonatal (HDFn). Methods : After treatment of HDFn with FLLW, MTT assay was performed to quantitatively determine cellular viability. The ERK and Akt pathways were analyzed in vitro by Western blot in a HDFn. HDFn proliferation after FLLW and minoxidil treatment in the absence or presence of PD98059, a MEK inhibitor, LY294002, and a PI3K inhibitor, was examined by Western blot or MTT assay. Results : FLLW increased cell proliferation in a dose-dependent manner and minoxidil used as positive control also induced cell proliferation in HDFn. FLLW increased the phosphorylation of ERK and Akt. In addition, minoxidil, too, induced the phosphorylation of ERK and Akt in HDFn. PD98059 and LY294002 significantly attenuated FLLW-inducible p-ERK and p-Akt expression and proliferation in cultured HDFn. Conclusions : Our results suggest that FLLW stimulates the growth of fibroblast cells through ERK and Akt pathways. Therefore, FLLW is a potential agent for the inducer of fibroblast growth.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2004년도 제4회 발생공학 국제심포지움 및 학술대회
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• pp.26-31
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• 2004

This work was undertaken in order to study the developmental competence of nuclear transfer cat embryo with fetal fibroblast and adult skin fibroblast as donor nuclei. Oocytes wererecovered by mincing the ovaries in Hepes-buffered TCM199 and selected the cumulus oocyte complexes (COCs) with compact cumulus cell mass and dark. Homogenous ooplasm were cultured for maturation in TCM199 + 10% fetal bovine serum (FBS) for 12 hours and used as a source of recipient cytoplast for exogenous somatic nuclei. In Experiment 1, we evaluated the effect donor cell types on the reconstruction and development of cloned embryos. Fusion, first cleavage and blastocyst developmental rate was not different between fetal fibroblast and adult skin cell (71.2 vs. 66.8; 71.0 vs. 57.6; 4.0 vs. 6.1 %, P<0.05). In Experiment 2, cloned embryos were surgically transferred into the oviducts of recipient queens. One of seven recipient queens was delivered naturally 2healthy cloned cats and 1 stillborn from fetal fibroblast cell of male origin after 65 days embryo transfer. One of three recipient queens was delivered naturally 1 healthy cloned cat from adult skin cell of female after 65 days embryo transfer. The cloned cats showed genotypes identical to the donor cell lines, indicating that adult somatic cells can be used for feline cloning.

• 대한화장품학회지
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• 제24권3호
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• pp.31-37
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• 1998

Soybeans are one of the major crops for human food resource; protein, lipid, and carbohydrate. In these days, they are widely using for cosmetics to supply phospholipid; natural surfactant. In this study we used black soybean seed in korea and observed many kinds of biochemical constituents; isoflavone, melatonin, crisantemine and calcium in ethanol extract. Also, its extract (we named it Flatonin) has been demonstrated that korean black soybean seed is able to stimulate the proliferation of NIH 373 cells and increase the production of type III collagen in NIH 373 and Malme-3 (human skin fibroblast) cells. The addition of korean black soybean to quiescent NIH 373 cells resulted in an increase of proliferation which was assayed by MTF method. The maximum effect of korean black soybean was detected in 0.4％ korean black soybean treated cells which was comparable to that of 5% serum(96％ of 5％ serum effect). The addition of korean black soybean to NIH 373 and Malme-3 cells also increased the production of type III collagen in both cells. These results indicate that korean black soybean may enhance the repair process after injury and prevent aging processes in connective tissues.

• International Journal of Oral Biology
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• 제30권3호
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• pp.105-110
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• 2005

Periodontitis is a chronic infectious disease that leads to the destruction, one of the major cause of tooth loss in human. Osteoclast Differentiation Factor(ODF), also called as Receptor activator of NF-${\kappa}B$ ligand(RANKL), a surface-associated ligand on bone marrow stromal cells and osteoblasts, activates its cognate receptor RANK on osteoclast progenitor cells, which leads to differentiation of these mononucleated precursor cells. Osteoprotegerin(OPG), a decoy receptor, is released from stromal cells and osteoblasts to inhibit the interaction between RANKL and RANK. The experiment for the effect of pregnancy on gingival health showed greater gingival inflammation and edema during pregnancy, despite similar plaque index. There should be many factors affecting the periodontal health in pregnancy. In this experiment, we examined the direct effects of sex hormones(estrogen and progesterone) on the ODF/OPG expression in human gingival fibroblasts and periodontal ligament cells at the serum concentration of pregnancy. The ratio was high in the 1st trimester of pregnancy by estrogen and in the late 2nd trimester by progesterone. Therefore, the local periodontal destruction might be accelerated by these hormonal effect on the periodontal cells.

• 한국식품영양과학회지
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• 제33권4호
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• pp.633-640
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• 2004

녹차분말을 포함하는 차가버섯 조성물의 수용성 추출물에 의한 인체 위암 세포 AGS 및 대장암 세포 HCT-15, 그리고 마우스 정상세포 NIH3T3 fibroblast의 세포생육에 미치는 효과를 세포 수 측정방법과 MTT assay 방법으로 측정하였다. 차가버섯 조성물의 수용성 추출물은 인체 대장암 세포주 HCT-15와 위암 세포주 AGS의 생육을 억제하였다. 그러나 동일한 실험조건 하에서 마우스 정상 세포주 NIH3T3은 80% 이상의 생존율을 나타내었다. 본 연구의 결과로 차가버섯 조성물의 수용성 추출물은 정상세포에는 독성을 나타내지 않으면서 위암 및 대장암 세포에는 높은 생육억제 효과를 나타냄을 알 수 있었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제27권1호
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• pp.1-8
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• 2001

This study was designed to localize the distribution of basic fibroblast growth factor(bFGF) in the developing rat condylar region and to elucidate the associated function of bFGF in the condyle development. The condyles of temporomandibular joint of Sprague-Dawley rats (27g of weight) were used. The tissues were examined with electron microscope and immunohistochemical method. The results were as follows: 1. The developing condylar region are divided in to 5 zones apparently: proliferative, maturation, hypertrophic, calcifying, and ossification zones. 2. The cells in the proliferative zone are condensed and have under-developed cell organells in the cytoplasm. This zone shows a strong immunoreactivity of bFGF. 3. The cells in the maturation zone are typical chondroblasts showing well-developed cell organells and round nucleus. The cartilaginous matrix does not show the immunoreactivity of bFGF, while the chondroblasts show the immunoreactivity. 4. The cells in the hypertrophic zone show hypertrophic change having the degenerated cell organelles and small nucleus. There are no immunoreactivity of bFGF in this zone except the nucleus and endoplasmic region showing mild immunoreactivity. 5, The cells in the calcifying zone show hypertrophic change and cell organelles are disappeared. The cells are surrounded by the calcified cartilaginous matrix. There are no immunoreactivity of bFGF in this zone except the endoplasmic region showing mild immunoreactivity. 6. In the zone of bone formation, chondroblasts are disappeared. Newly differentiated osteoblasts secreting osteoid around the calcified cartilaginous matrix. The bone marrow shows the immunoreactivity of bFGF, while the bone matrix does not show the immunoreactivity of bFGF.

• 생명과학회지
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• 제14권2호
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• pp.263-268
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• 2004

본 연구에서는 FGF-2를 이용하여 혈관내피세포의 발아와 단백질분해효소의 분비 및 인테그린과의 관계를 관찰하여 다음과 같은 결과를 얻었다. PPAECs 세포의 발아에 미치는 FGF-2의 효과를 조사한 결과, 10 ng/ml에서 약 3.5배 증가되는 등 농도-의존적으로 발아가 증가되었다. MMPs에 대한 enzyme immunoassay결과, MMP-2에서만 약 1.9배의 분비증가가 유도되었다. MMP-1 및 MMP-3는 FGF-2에 의해 유의할만한 분비 증가가 나타나지 않았으며, 특이하게 MMP-3는 FGF-2의 처리 없이도 많은 양이 분비되었고, MMP-9은 0.6∼1.2 ng/$10^{6}$ cells 정도로 분비량이 적었다. FGF-2에 의한 플라스민의 분비증가 여부를 확인한 결과, 10 ng/ml에서 약 2.6배 증가하였으며, MMPs 억제제 및 인테그린 억제제에 의해 유의할만한 감소가 나타났고 플라스민 억제제인 $\alpha$2-antiplasmin에 의해서는 플라스민의 분비가 완전히 억제되었다. 또한, FGF-2 처리에 의해 농도-의존적으로 인테그린 Mac-1의 발현이 증가되었으며, 인테그린 억제제인 IS201를 전처리한 결과, 인테그린 Mac-1의 발현이 완전히 억제되었다. FGF-2에 의한 발아 유도효과는 IS201 처리에 의해 거의 완벽하게 억제되었으며, MMP-2 및 플라스민의 분비도 유의할만하게 감소시켰다. 이상의 결과를 요약하면, FGF-2에 의해 유도된 혈관내피세포의 발아 증가는 MMP-2 및 플라스민의 분비증가와 인테그린 Mac-1의 발현 증가에 의한 것이라 생각된다.

• Clinical and Experimental Reproductive Medicine
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• 제24권3호
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• pp.355-359
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• 1997

Leiomyomas, which are the commonest pelvic tumors in women, are originated from myometrial cells. Although the exact initial pathophysiologic event of the leiomyoma is not known, recent evidences suggested that the effects of sex steroid hormones in the process of tumor growth are mediated by local production of growth factors including epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II). If we look at the effects of other cytokines, it was suggested that basic fibroblast growth factor (bFGF) may stimulate the proliferation of myometrial and leiomyomas cells. And it was reported that interferon-${\alpha}$ inhibit the action of bFGF. Therefore, we examined the effect of bFGF and interferon-${\alpha}$ on the proliferation of leiomyoma and myometrial cells. bFGF stimulated the myometrial and leiomyoma cells significantly at the concentration of 1ng/ml (p<0.05) and 5ng/ml (p<0.05). However, Interferon-${\alpha}$ inhibited the cell proliferation of myometrial and leiomyoma cells significantly at the concentration of 100U/ml (p<0.05) and 1000U/ml (p<0.05). And the stimulated effects of bFGF with the various concentration on the myometrial and leiomyoma cells ware inhibited by interferon-${\alpha}$ with 100U/ml. Therefore, we concluded that bFGF may stimulate the myometrial and leiomyoma cell proliferation and interferon-${\alpha}$ may inhibit the myometrial and leiomyoma cell proliferation through blocking the effect of basic fibroblast growth factor.