• Title/Summary/Keyword: Fiber array

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Selection of Resistant Varieties to Aspergillus flavus by Determination of Aflatoxin B1 Content in Korean Peanut (Arachis hypogaea L.) Accessions

  • Seungah Han;Byeong-Cheol Kim;Jungmin Ha;Tae-Hwan Jun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.3
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    • pp.175-187
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    • 2023
  • Peanuts, also known as groundnuts (Arachis hypogaea L.), are globally recognized as a vital oilseed crop. Peanuts are rich in proteins (e.g., arginine), oils (e.g., oleic acid and linoleic acid), fiber, vitamins (e.g., niacin and tocopherol), and carbohydrates and are consumed worldwide. However, the presence of aflatoxin (AF) has garnered substantial attention since its initial discovery as the causative agent of Tukey's X disease in the United Kingdom in 1960. Among the 18 aflatoxins identified, aflatoxin B1 (AFB1) has the highest toxic activity and causes hepatocellular carcinoma. It is classified as Group I by the International Agency for Research on Cancer (IARC) of the World Health Organization (WHO). The present study was conducted to evaluate aflatoxin B1 resistance of 102 peanut accessions and select putative aflatoxin B1-resistant peanut accessions to aflatoxin B1. One hundred and one Korean germplasms harvested in 2020 were inoculated with A. flavus to identify aflatoxin-resistant cultivars, and the aflatoxin B1 concentration was measured using an ultra-performance liquid chromatography-photodiode array detector. Twenty-six accessions with aflatoxin B1 concentrations lower than those of the check plant 55-437 were chosen for the development of aflatoxin-resistant varieties in Korea. As Korean aflatoxin-resistant varieties have not yet been developed, the findings of the present study are expected to provide useful information for the development of aflatoxin-resistant cultivars.

Sensitivity Evaluation and Approximate Optimization Analysis for Structure Design of Module Hull Type Trimaran Pontoon Boat (모듈 선체형 삼동 폰툰 보트의 구조설계 민감도 평가와 근사 최적화 해석)

  • Bo-Youp Choi;Chang-Ryeon Son;Joon-Sik Son;Min-Ho Park;Chang-Yong Song
    • Journal of the Korean Society of Industry Convergence
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    • v.26 no.6_3
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    • pp.1279-1288
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    • 2023
  • Recently, domestic leisure boats have been actively researching eco-friendly product development to enter the global market. Since the hulls of existing leisure boats are mainly made of fiber reinforced plastic (FRP) or aluminum, design techniques for securing structural safety by applying related materials have been mainly studied. In this study, an initial structural design safety assessment of a trimaran pontoon leisure boat with a modular hull structure and eco-friendly high-density polyethylene (HDPE) material was conducted, and sensitivity evaluation and optimization analysis for lightweight design were performed. The initial structural design safety assessment was carried out by creating a finite element analysis model and applying the loading conditions specified in the ship classification regulation to check whether the specified allowable stresses are satisfied. For the sensitivity evaluation, the influence of stress and weight of each hull structural member was evaluated using the orthogonal array design of experiments method, and an approximate model based on the response surface method was generated using the results of the design of experiments. The optimization analysis set the thickness of the hull structural members as the design variable and considered the optimal design formulation to minimize the weight while satisfying the allowable stress. The algorithm of the optimization analysis applied the Gradient-population Based Optimizer (GBO) to improve the accuracy of the optimal solution convergence while reducing the numerical cost. Through this study, the optimal design of a newly developed eco-friendly trimaran pontoon leisure boat with a weight reduction of 10% was presented.

Signal Analysis of Optical Biosensor to Detect Peroxide Using Electrically Controlled Release System (전기적 방출 조절 시스템을 이용한 광 페록사이드센서의 개발)

  • Min, Jun-Hong;Lim, In-Hee;Kim, Hyo-Han;Lee, Sang-Beak;Choi, Jeong-Woo;Lee, Won-Hong
    • Journal of Sensor Science and Technology
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    • v.6 no.1
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    • pp.35-42
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    • 1997
  • The optical biosensor using the electrically controlled release of reactive reagent is developed for the detection of peroxide. Rapid degradation of polymer complex of PEOx and PMAA occurs as the applied current increases and thus released amount of HPA increases. The degradation velocity of polymer and the amount of HPA released are linearly proportional to the applied current. Peroxide is reacted with the released reagent by peroxidase and then the product, a fluorescent dimer DBDA, is formed. The monochromic light from light source (150W Xe arc ramp) excites the DBDA and the excited light is transmitted through an optical fiber to be detected by a photodiode array. The change of fluorescence intensity is related to the change of peroxide concentration. The peroxidase is entrapped in Ca-alginate get on the inner surface. The biosensor has the linear signal range of 0.025mM-10.mM peroxide. By applying the step function of peroxide, reproducibility of biosensor has been investigated. The mathematical model is constructed by the combination of enzyme kinetics with reactor flow model. Good agreement is obtained between the experimental result and model prediction in the sensor signal.

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THE NONDESTRUCTIVE MEASUREMENT OF THE SOLUBLE SOLID AND ACID CONTENTS OF INTACT PEACH USING VIS/NIR TRANSMITTANCE SPECTRA

  • Hwang, I.G.;Noh, S.H.;Lee, H.Y.;Yang, S.B.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2000.11b
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    • pp.210-218
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    • 2000
  • Since the SSC(soluble solid contents) and titratable acidity of fruit are highly concerned to the taste, the need for measuring them by non-destructive technology such as NIR(Visual and Near-infrared) spectroscopy is increasing. Specially, in order to grade the quality of each fruit with a sorter at sorting and packing facilities, technologies for online measurement satisfying the tolerance in terms of accuracy and speed should be developed. Many researches have been done to develop devices to measure the internal qualities of fruit such as SSC, titratable acidity, firmness, etc. with the VIS(Visual)/NIR(Near Infrared) reflectance spectra. The distributions of the SSC, titratable acidity, firmness, etc. are different with respect to the position and depth of fruit, and generally the VIS/NIR light can interact with fruit in a few millimeters of pathlength, and it is very difficult to measure the qualities of inner flesh of fruit. Therefore, to measure the average concentrations of each quality factor such as SSC and titratable acidity with the reflectance-type NIR devices, the spectra of fruit at several positions should be measured. Recently, the interest about the transmittance-type VIS/NIR devices is increasing. NIR light can penetrate through the fruit about 1/10-1/1,000,000 %. Therefore, very intensive light source and very sensitive sensor should be adopted to measure the transmitted light spectra of intact fruit. The ultimate purpose of this study was to develop a device to measure the transmitted light spectra of intact fruit such as apple, pear, peach, etc. With the transmittance-type VIS/NIR device, the feasibility of measurement of the SSC and titratable acidity in intact fruit cultivated in Korea was tested. The results are summarized as follows; A simple measurement device which can measure the transmitted light spectra of intact fruit was constructed with sample holder, two 500W-tungsten halogen lamps, a real-time spectrometer having a very sensitive CCD array sensor and optical fiber probe. With the device, it was possible to measure the transmitted light spectra of intact fruit such as apple, pear and peach. Main factors affecting the intensity of transmitted light spectra were the size of sample, the radiation intensity of light source and the integration time of the detector. Sample holder should be designed so that direct light leakage to the probe could be protected. Preprocessing method to the raw spectrum data significantly influenced the performance of the nondestructive measurement of SSC and titratable acidity of intact fruit. Representative results of PLS models in predicting the SSC of peach were SEP of 0.558 Brix% and R2 of 0.819, and those in predicting titratable acidity were SEP of 0.056% and R2 of 0.655.

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Quali-Quantitative Analysis of Flavonoids for Mulberry Leaf and Fruit of 'Suhyang'

  • Ju, Wan-Taek;Kwon, O-Chul;Lee, Min-Ki;Kim, Hyun-Bok;Sung, Gyoo-Byung;Kim, Yong-Soon
    • Korean Journal of Environmental Agriculture
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    • v.36 no.4
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    • pp.249-255
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    • 2017
  • BACKGROUND: Globally, mulberry (Morus sp.) is exploited for feeding leaf to silkworms in order to obtain silk fiber or for animal feedstock production. Also, mulberry fruit is known to a by-product that was produced from mulberry tree after harvesting leaves for silkworm rearing, as a yield and consumption of mulberry fruit was increased, it has been fixing to a newincome crop. Mulberry leaves and fruits are used for the health benefits of human beings. Mulberry contains various bioactive components, such as alkaloids and flavonoids. Mulberry flavonoids are an important part of the diet because of their effects on human nutrition. The flavonoids in mulberry leaf and fruit of 'Suhyang'(Morus alba L.) were determined. METHODS AND RESULTS: Flavonoids for mulberry leaf and fruit of 'Suhyang' were analysed using ultrahigh performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS)technique. An UPLC-DAD-QTOF/MS system was used, and identification of mulberry leaves constituents was carried out on the basis of the complementary information obtained from LC spectra, MS ions, and MS/MS fragments. The mulberry leaf (16 flavonoids) and fruit (9 flavonoids) were isolated and analyzed from Suhyang using UPLC-DAD-QTOF/MS chromatogram. To the best of our knowledge, Quercetin 3-O-(6"-O-malonyl) glucoside and quercetin 3-O-rutinoside (rutin) was detected on the highest content in leaf and fruit, respectively and further research will be devoted to evaluate their biological activity. CONCLUSION: Obtaining information about the concentration of functional materials in mulberry leaves could contribute to the development and promotion of processed, functional products and offer possible industrial use of 'Suhyang', holding promises to enhance the overall profitability of sericulture.

Luzhong mutton sheep: inbreeding and selection signatures

  • Tao, Lin;He, Xiaoyun;Wang, Fengyan;Zhong, Yingjie;Pan, Linxiang;Wang, Xiangyu;Gan, Shangquan;Di, Ran;Chu, Mingxing
    • Journal of Animal Science and Technology
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    • v.62 no.6
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    • pp.777-789
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    • 2020
  • Intense artificial selection has been imposed to Luzhong mutton sheep population in the past years. Improvements on growth and reproductive performance are two breeding goals in the present herd. Although some progresses were phenotypically observed possibly due to inbreeding induced by strong selection in terms of these traits, the genomic evaluation was poorly understood. Therefore, a high-density SNP array was used to characterize the pattern of runs of homozygosity (ROH), estimate inbreeding and inbreeding depressions on early growth performance and litter size based upon ROH, and scan positive selection signatures of recent population. Consequently, a low inbreeding level was observed which had negative effects on litter size, but not on early growth performance. And 160 genes were under selection, of which some were reported to be linked to several traits of sheep including body weight, litter size, carcass and meat quality, milk yield and composition, fiber quality and health, and the top genes were associated with growth (growth hormone [GH]- growth hormone receptor [GHR]- Insulin-like growth factor 1 [IGF1] axis) and litter size (bone morphogenic proteins [BMPs]-associated). The effectiveness of previous breeding measures was highlighted, but purging selection was proposed to alleviate the inbreeding depression on litter size, providing some genomic insights to breeding management of Luzhong mutton sheep.