• Clinical and Experimental Reproductive Medicine
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• v.11 no.2
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• pp.59-67
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• 1984

In vitro fertilization have been performed to know whether the frozen semen has fertilizing ability and can be used clinically. The results of cultured and developed embryos obtained are as follows: 1. The semen was frozen in three media for the good viability. The viability was more than 50% and the motility was also moderate (grade III), 2. As the 33 oocytes were collected from 45 follicles, the oocyte recovery rate was 73.3%. Among them, mature and immature ova were 5% each, and premature ova were 69.7%, When the first polar body was appeared, above ova were inseminated after adequate incubation with activated sperms. 3. The main components of three freezing medium containing egg yolk, glycerol and pyruvate respectively were the best for sperm viability, and Ham's F-10 medium was used for the fertilization and culture of eggs. 4. The results of in vitro fertilization of 33 ova, showed the second polar body developed in 12%, polyspermia in 24%, 1-cell embryo in 21% and 2-cell embryo in 9%. One mature ova developed to blastocyst via 16-cell to 32-cell embryo. The fertilization rate was 66%. 5. Above mentioned results represent that the frozen semen has fertilizing ability and can be used practically in the clinic.

• Korean Journal of Environment and Ecology
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• v.11 no.1
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• pp.37-45
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• 1997

The Nogodan area has maintained the subalpine meadows(m.s.l. 1,500m) in Chirisan National Park. A field experiment including fertilizing, introducing plants and mulching treatment was conducted for three years at artificially disturbed subalpine meadows in the Nogodan to find out effective revegetation measures. Factorial experiment(2*2*2) was applied to the subsoil sites with ten percent hillslope and the topsoil sites with forty percent hillslope. Regardless of site conditions, survival rates of plants were more effective in on-site conditions, showed significant increase in the number of individuals and crown coverage of vegetation, but mulching treatment did not show a significant effect.

• Journal of Environmental Science International
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• v.24 no.10
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• pp.1253-1264
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• 2015

This study assessed the fertilizing value of struvite deposit recovered from swine wastewater in cultivating Chinese cabbage. Struvite deposit was compared with commercial fertilizers: complex, organic and compost to evaluate the fertilizing effect of struvite deposit. Laboratory pot test obviously presented that the struvite deposit more facilitated the growth of Chinese cabbage than organic and compost fertilizers even though complex fertilizer was the most effective in growing Chinese cabbage. It was revealed that the growth rate of Chinese cabbage was simultaneously controlled by phosphorus (P) and potassium (K). Also, the nutrients such as nitrogen (N), P, K, calcium (Ca) and magnesium (Mg) were abundantly observed in the vegetable tissue of struvite pot. Specifically, P was the most abundant component in the vegetable tissue of struvite pot. Meanwhile, the utilization of struvite as a fertilizer led to the lower accumulation of chromium ($Cr^{6+}$) than other pots, except for compost fertilizer pots, and no detection of cadmium (Cd), arsenic (As) and nickel (Ni) in the Chinese cabbage. The experimental results proved that the optimum struvite dosage for the cultivation of Chinese cabbage was 2.0 g struvite/kg soil. On the basis of these findings, it was concluded that the struvite deposits recovered from swine wastewater were effective as a multi-nutrient fertilizer for Chinese cabbage cultivation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.97-101
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• 1991

This experiment was carried out to determine the effect of pure mineral nitrogen fertilization on dry matter yield and the amounts of advisable mineral nitrogen on grassland according to its age at the "Federal Institue for Agriculture in the Alps" in Austria. The results obtained were as follows: 1. With only PK-fertilizing average dry matter yields per year were progressively declined during the period of the studies. Compared with the relatively younger grassland(1st-5th year) the DM-yields were decreased by 35% in older grassland (11th-final year). 2. Due to the less reduction of DM-yields by mineral nitrogen application according to the age of grassland. With N-fertilizing the reduction of DM-yields in relatively older grassland was less than that of younger grassland by 11-21%. 3. At 4-and 5-cut systems the nitrogen amounts for the highest marginal yield(the "most efficient" Ndressing rate) per ha and year were distinctly declined in the relatively older grassland. 4. Required efficiencies of mineral nitrogen were not influenced by the age of grassland.he age of grassland.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.4
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• pp.355-360
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• 1992

The experiment was carried out to study the influence of planting densities and fertilizer application levels on some agronomic characters and yield of flower-head in Zingiber mioga ROSC. The results obtained are summarized as follows ; Plant hight was long in dense planting, and short in spacious planting. Stem diameter was thick in spacious planting, and was thin in dense planting. Flower-head weight per plant was decreased in dense planting and was increased in spacious planting. Yield was higher in dense planting densities (10$\times$20 : 50plants /m$^2$, 20$\times$20 : 25plant /m$^2$). Fertilizing (N : P$_2$O$_{5}$ : $K_2$O=15 : 15 : 15kg /10a) was increased than without fertilizing because plant height and number of stem was good.d.

• Clinical and Experimental Reproductive Medicine
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• v.16 no.1
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• pp.15-21
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• 1989

An in vitro fertilization assay employing zona-free hamster embryos was used to investigate human spermatozoal fertilitzing ability. Yanaghimarchi et al.(1976) first introduced this cross species fertilzation technique, with its application as a diagnostic tool for male infertility. Human spermatozoa were preincubated for 3 to 4 hrs in B W W medium at concentration of $4{\times}10^6$ sperm/ml prior to the addition to zona-free hamster embryos. After 3 hrs, human sperm was evaluated for fertilizing potential by the presence of swelling or decondencing sperm head in the cytoplasm. The results of penetration rates for sperm were as follow : 1. The average penetration rate of a 7 fertile donor group was $47.8{\pm}27.67%$(Range 14.3-98.0%) 2. The average penetration rate of 12 infertile patients with normal semen analysis was $21.7{\pm}26.9%$(Range 0-38.8%) 3. The average penetration rate of 10 infertile patients with semen abnormalities was $6.1{\pm}8.1%$(Range 0-25%)

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.8
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• pp.1196-1202
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• 2001

In boar spermatozoa, the head-to-head agglutinability changes in parallel with the development of the fertilizing ability. Namely, both abilities gradually increase in the distal caput and corpus epididymides, but are subsequently suppressed in the cauda epididymidis. It has been postulated that these changes of the agglutinability are controlled via sperm interaction with specific epididymal plasma factors including agglutination mediators (agglutinins) and inhibitors (anti-agglutinins). Expression of these abilities (sperm agglutination and capacitation) is hardly observed in spermatozoa immediately. after ejaculation, but it occurs during incubation in a capacitation medium. Recently, we have purified and characterized epididymal plasma anti-agglutinin for boar spermatozoa. Moreover, we have conducted a series of experiments to reveal biological significance and mechanism of the head-to-head agglutination and have accumulated data indicating that boar sperm agglutination is mediated by capacitation-supporting factors including calcium, bicarbonate and sterol acceptors. This review introduces our recent data and discusses a possible mechanism for suppression of the agglutinability in the distal epididymidis and relationship between agglutinability and fertilizing ability.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.6
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• pp.609-613
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• 1997

The reproductive performance of forty two male broilers divided into three similar groups and fed on isocaloric and isonitrogneous diets containing 0, 10 or 20% water washed neem seed kernel meal (WWNSKM) was investigated from 20 to 32-wks of age. Results on semen characteristics revealed that feeding of WWNSKM led to significant (p < 0.05) reduction in semen volume, sperm concentration associated with increased incidences of morphological abnormalities in the spermatozoa when compared to that of the control birds. A drastic reduction in the fertilizing ability of spermatozoa was observed, the adverse effects being more at higher inclusion level of the cake. Hatchability of eggs also declined in the WWNSKM fed group. Histological examination of testes revealed a higher number of degenerating cells and poor spermatogenesis along with multinucleated giant cells in the seminiferous tubules of the testes of birds receiving the high dose of WWNSKM in diet. It may be concluded that the feeding of WWNSKM by incorporating in isocaloric and isonitrogneous diets to cockerels is associated with adverse effect on their fertility.

• Korean Journal of Organic Agriculture
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• v.12 no.2
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• pp.209-217
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• 2004

The aim of this experiment was to investigate the effects of applying times and dilution rates of cattle slurry on dry matter yields of orchard grass. Cattle slurry was applied at the rates of average mineral nitrogen fertilizer equivalent to 150 kg/ha/year in 3 cutting frequency. Significantly higher dry matter yields than that of no fertilization (3.04 ton DM/ha) were recorded in the application of diluted cattle slurry(5.38~6.25 ton DM/ha) (p<0.05). Especially, this tendency was shown with higher annual dry matter yields at the partitioned dressing times, such as at the applications for 1st and 2nd growth, 1st and 3rd growth, and 1st, 2nd and 3rd growth respectively. The yields of annual dry matter both at fertilizing phosphorus and potassium, and phosphorus, potassium and nitrogen were higher than no fertilization as 5.41 tons and 8.78 tons per ha respectively. However, with application of diluted cattle slurry, dry matter yield par year (5.84 ton DM/ha) was higher than those of fertilizing phosphorus and potassium. The efficiencies of dry matter production with mineral and cattle slurry nitrogen application (kg DM/kg N) were 225 and 15.8~18.7 kg DM/kg N respectively. Especially, these tendencies were higher in diluted application plots than in no-diluted application plots of cattle slurry.

• Journal of Animal Environmental Science
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• v.19 no.2
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• pp.183-190
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• 2013

Establishment of a new concept of environmental friendly livestock manure management is required based on the facts. But now liquid fertilizer quality in korea shows a large difference among regions and the regulations are uncertain. Focusing on precedent study on main level-grading factors of liquid fertilizer quality certification, the study collected laws and standards related to liquid fertilizer of livestock manure at home and abroad and produced evaluation standards. Liquid fertilizer was divided into four factors (fertilizing value, harmfulness, stability and uniformity). According to each item, scores were awarded based on 16 details: fertilizing value (Nitrogen concentration, the whole concentration of Nitrogen, Phosphoric acid and Kalium), harmfulness (heavy metals, pathogenic microorganism and antibiotics), stability (maturity degree and odour), uniformity (EC, BOD, SS, moisture content and salt). The grade of liquid fertilizer, A (42~48), B (34~41), C (26~33) were rated using total scores.