• Title/Summary/Keyword: Fertilized egg

Search Result 266, Processing Time 0.039 seconds

Effect of Temperature, Salinity and Density on the Egg Development of the Sunray Surf Clam, Mactra chinensis (개량조개, Mactra chinensis의 난발생에 미치는 수온, 염분 및 수용밀도의 영향)

  • Min, Byeong-Hee;Kim, Tae-Jin
    • The Korean Journal of Malacology
    • /
    • v.26 no.4
    • /
    • pp.297-302
    • /
    • 2010
  • Water temperature, salinity and density on egg development of the sunray surf clam, Mactra chinensis were investigated for artificial seedling production. The required time from fertilization to D-shaped larvae were 33.8 hours in $18^{\circ}C$, 20.6 hours in $23^{\circ}C$, 18.2 hours in $28^{\circ}C$ and 15.0 hours in $33^{\circ}C$. The development duration was reduced with increasing temperature. The relationships between temperature and the required time from egg to each developmental stage were described as follows: 2-cell, 1/h = 0.1051WT - 1.4782; 8-cell, 1/h = 0.037WT - 0.3686; gastrula, 1/h = 0.008WT - 0.0521; trochophore, 1/h = 0.0041WT - 0.0235; D-shaped larva, 1/h = 0.0024WT - 0.0102. Biological minimum temperature for the egg development was estimated to be $8.0^{\circ}C$ in average. The possible range of temperature for the development of D-shaped larvae was $18-28^{\circ}C$ and optimum of water temperature for the development of egg was $23^{\circ}C$. The possible range of salinity for the development of D-shaped larvae was 20-35 psu and optimum of salinity for the development of egg was 30-35 psu over 25 psu at least. The density of fertilized egg was below 40 per 1 ml in rearing seawater for elevating the development rate from fertilized egg to D-shaped larva.

Development and growth in fertilized eggs and larvae of the Japanese geoduck, Panopea japonica reared in the laboratory (코끼리조개, Panopea japonica (A. Adams)의 수정란 발생과 유생 성장)

  • Nam, Myung-Mo;Lee, Chu;Kim, MeeKyung;Kim, Jae Won;Kim, Young Dae
    • The Korean Journal of Malacology
    • /
    • v.30 no.4
    • /
    • pp.303-309
    • /
    • 2014
  • The development of Japanese geoduck, Panopea japonica, grown under culture conditions, has been examined through the morphological characteristics in fertilized egg, larvae and juvenile. Gametes were stripped from ripe broodstock and placed into two separate containers. Eggs were washed through a $40{\mu}m$ sieve and fertilized with dilute sperm solution. Developing larvae were maintained at $19{\pm}1^{\circ}C$. Fertilized eggs with $81.6{\mu}m$ diameter developed to trochophores within 14 h and to D-stage larvae ($116{\mu}m$ shell length) within 27 h. Larvae were spontaneously settled at shell length of $311{\mu}m$ after 20 days. The hatching from fertilized eggs and larval rearing were normally available in $18.5-21.5^{\circ}C$, and the growth was good in a cashmilon substrate, as well as sand. After rearing of day 108 from metamorphosis, the shell length of juvenile P. japonica reached 13 mm, and growth rate of shell length of the juvenile was $117.5{\mu}m/d$.

Effects of 3,5,3'-triiodo-L-thyronine (T3) Immersion on Development of Fertilized Egg and Newly Hatching Larva of Black Seabream, Acanthopagrus schlegeli (감성돔, Acanthopagrus schlegeli 수정란과 부화자어의 발달에 미치는 3,5,3'-triiodo-L-thyronine (T3)의 침지처리 효과)

  • 강덕영
    • Journal of Life Science
    • /
    • v.13 no.1
    • /
    • pp.34-41
    • /
    • 2003
  • The hatching of fertilized egg and early development of larval black seabream, Acanthopngrus schlegeli by immersion of 3,5,3'-triiodo-L-thyronine ($T_3$) with doses of 0.01~0.5 ppm in tank were examined for early development periods and compared with control fish immersed by 0.1 N NaOH and 99% alcohol. Larvae were fed with rotifers for first 5 days during experimental period. Yolk absorption of larvae from $T_3$ immersion groups were significantly faster than that of control. Although hatching times among each groups were not significant difference. the hatching rates of larvae in higher doses (0.1 ppm and 0.5 ppm $T_3$ groups) were significantly lower than control. The results of $T_3$ immersion experiments to newly hatching larvae indicated that exogenous $T_3$induced significant faster the absorption of yolk and oil globule, faster the development of myotome and digestive duct and higher the growth rate of larvae than larvae of control. These results indicate that $T_3$ immersion supplements appear to confer a distinct advantage to eggs and larvae of black seabream, which is in early fragile developmental stage.

Spawning and Hatching of Octopus minor (낙지 (Octopus minor)의 산란과 부화)

  • Kim, Dong-Soo;Kim, Jae-Man
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.40 no.4
    • /
    • pp.243-247
    • /
    • 2007
  • We investigated the reproductive behavior of Octopus minor, order Octopoda, class Cephalopoda under laboratory conditions. Each mature female octopus was kept in an aquarium with a plastic tube for shelter, and one mature male was introduced for the purpose of copulation. Before spawning, the female coated the roof of the shelter with a light-green material, upon which it then fixed its eggs one by one. This spawning behavior lasted 1 to 3 days. Fertilized females spawned 54 eggs on average, ranging from 21 to 112 eggs at 72 to 98 days after copulation. The attached eggs were 18.1-19.0 mm in length, 5.0-6.1 mm in width, and 0.30-0.38 g in weight. The mother octopods did not feed; they attended to the eggs by using their arms to rub the egg surfaces and used their funnel to blow sediments off of the eggs. At water temperatures of $20.9-21.5^{\circ}C$, the fertilized eggs hatched within 73 to 90 days after being spawned. The effective cumulative water temperature was $1,569-1,892^{\circ}C$. At the end of incubation, the body weight of the mother octopods was reduced to approximately 56% of the initial weight, and most mother octopods died soon after the young hatched.