• Monthly Duck's Village
• /
• s.86
• /
• pp.57-61
• /
• 2010

이번 연구는 아플라톡신 $B_1(AFB_2)$의 독성이 오리의 생산성, 체내 기관, 간 효소 활성도, 외관상 소화율, 영양소 소화율에 미치는 영향을 알아보기 위한 것이다. 1일령의 육용오리 90마리를 3개의 처리군으로 나눠 10마리씩 펜에서 사육하였다. 그룹1은 일반 사료를 급여하였고, 그룹 2와 3은 각각 아플라톡신 $20{\mu}g/kg$, $40{\mu}g/kg$이 포함된 오염된 쌀을 섞어 6주 동안 급여하였다. 그 결과 아플라톡신에 오염된 사료를 섭취한 그룹의 증체량과 사료 섭취량이 감소하였고, 사료요구률(feed to gain ratio), 간, 신장, 췌장의 무게가 높은 것으로 나타났다. 알라닌 아미노전이효소(ALT, serum alanine aminotransferase)와 혈중 아스파라진산 아미노전이효소(AST, aspartate aminotransferase)의 활성도도 아플라톡신 오염 그룹에서 유의성을 보이며 높았다. 아플라톡신 오염 그룹의 오리들의 십이지장에서 채취한 단백질 분해효소, 키모트립신, 트립신(이자액에서 분비되는 단백질 분해효소), 전분 가수 분해효소 등 소화효소의 활성도가 증가한 반면, 조단백질의 외관상 소화율은 유의성있게 낮은 것으로 나타났다. 이는 아플라톡신에 오염된 사료로가 오리의 생산성과 영양소의 외관상 소화율을 감소시키고 십이지장 내용물의 소화효소활성을 변화시킨다고 볼 수 있다.

• Journal of Wetlands Research
• /
• v.11 no.1
• /
• pp.15-27
• /
• 2009

To reveal conservation value of Sowhangbyungsan-neup among wetlands in Mt. Odae designated as a Ramsar convention site in 2008, ecological characteristics were investigated. The maximum depth of sediment was 86cm and average at central part was 56cm. Average pH of water was 5.8 and this indicates that this wetland has the characteristics between bog and fen. Average electron conductivity was $11{\mu}S$/cm, which is the lowest among Korean wetlands. Nutrient status was oligotrophic based on contents of cation, nitrate, ammonia, soluble reactive phosphorus in water. Out-flow of water was related with the 3 day cumulative precipitation. Soil texture was loam and nutrient level was very low. Vascular plants of 45 family, 95 genus, 121 species, 2 subspecies, 16 variety, 4 forma, total 121 taxa were recorded. Main plant communities on Sphagnum base were Osmunda cinnamomea community, Carex dispalata community and Carex curta community. The results indicated that this wetland has very different ecological characteristics than others in Korea and deserves conservation value well. Also, this study revealed that there is no evident threatening factor and this wetland will have characteristics of fen continuously in near future.

• BMB Reports
• /
• v.33 no.2
• /
• pp.155-161
• /
• 2000

The saccharomyces cerevisiae Rad27, a structure-specific endonuclease for the okazaski fragment maturation has been known to interact genetically and biochemically with Dna2, an essential enzyme for DNA replication. In an attempt to define the significance of the interaction between the two enzymes, we expressed and purified both Dna2 and Rad27 proteins. In this report, Rad27 could not form a complex with Dna2 in the three different analyses. The analyses included glycerol gradient sedimentation, protein-column chromatography, and coinfection of baculoviruses followed by affinity purification. This is in striking contrast to the previous results that used crude extracts. These results suggest that the interaction between the two proteins is not sufficiently stable or indirect, and thus requires an additional protein(s) in order for Rad27 and Dna2 to form a stable physical complex. This result is consistent with our genetic findings that Schizosaccharomyces pombe Dna2 is capable of interacting with several proteins that include two subunits of polymerase $\delta$, DNA ligase I, as well as Fen-1. In addition, we found that the N-terminal modification of Rad27 abolished its enzymatic activity. Thus, as suspected, we found that on the basis of the structure determination, N-terminal methionine indeed plays an important role in the nucleolytic cleavage reaction.

• Herbal Formula Science
• /
• v.9 no.1
• /
• pp.165-206
• /
• 2001

After this study, I report the following result from it 1. 'Qiao zhang' is the symptoms that the abdomen is distension and fullness, but face, eye, four limb is not dropsial swelling. It corresponds to the Gu zhang(?脹), Zhang man(脹滿), Jiju(積聚), Dan fu zhang(單腹脹), Zhong man(重滿) of the Oriental Medicine. 2. The cause and overeating and, and malfunction pathology of the 'Qiao zhang' were accumulation of water cause of overeating and, overdrinking, the hurt of feeling, overwork, infection of blood sucker and malfunction of liver, spleen and kidney for jandice and Jiji(積聚) 3. The treatment of the 'Qiao zhang' was reinforcing middle-energizer and replenishing Qi and allevating water retention because middle-energizer were much weak. 4. The prescription of the 'Qiao zhang' that Zhang man fen xiao wan(中滿分消散) were 16 times the most. Zhang man fen xiao tang(中滿分消湯), Guang man kui jian tang(廣茂潰堅湯), ren shen gui pi tang(人蔘芎歸湯), Yu yu liang wan(禹餘糧丸), mun xiang sun qi tang(木香順氣散) were next. 5. The single herb of the prescription of the 'Qiao zhang' that were Auckrandiae Radix(木香), Citri Pericarpium(陳皮) for rephenshing qi drug, Atractylodis Macrocephalae Phieoma(白朮), Ginseng Radix(人蔘), Magnoliae Cortex(厚朴) for regurating qi drug, Pinelliae Rhzoma(半夏) for dischanging phlegm drug.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.15 no.1
• /
• pp.53-62
• /
• 2012

Culm cutting is very effective and convenient method for asexual propagation and even much less destructive than the other ways such as excavation of rhizomes. Despite that culm cutting is such a useful method, only few investigations for raising its efficiency have been carried out. We tried to examine the effect of different geographical populations and cutting sections on the shoot emergence, biomass production and its allocation in terms of cutting efficiency. Culms were sampled from three environmentally distinct wetlands : riparian marsh, salt marsh and montane fen and then they were cut separately into four sections from the bottom to the top part. Both factors of different population and section affected the shoot emergence together. Middle parts on the culm always showed more shoot emergence irrespective of different population. On the other hand, first section from salt marsh and fourth section from the montane fen did not exhibit any shoot emergence. Significant difference in increase of shoot emergence between different sections during investigation period was confirmed only from salt marsh population. Not only different population but also appropriate cutting section should be considered together for better efficiency in cutting of reed culms.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.11
• /
• pp.5725-5728
• /
• 2012

Growing evidence shows that deregulation of the circadian clock plays an important role in the development of malignant tumors, including gliomas. However, the molecular mechanisms of gene chnages controlling circadian rhythm in glioma cells have not been explored. Using real time polymerase chain reaction and immunohistochemistry techniques, we examined the expression of two important clock genes, cry1 and cry2, in 69 gliomas. In this study, out of 69 gliomas, 38 were cry1-positive, and 51 were cry2-positive. The expression levels of cry1 and cry2 in glioma cells were significantly different from the surrounding non-glioma cells (P<0.01). The difference in the expression rate of cry1 and cry 2 in high-grade (grade III and IV) and low-grade (grade 1 and II) gliomas was non-significant (P>0.05) but there was a difference in the intensity of immunoactivity for cry 2 between high-grade gliomas and low-grade gliomas (r=-0.384, P=0.021). In this study, we found that the expression of cry1 and cry2 in glioma cells was much lower than in the surrounding non-glioma cells. Therefore, we suggest that disturbances in cry1 and cry2 expression may result in the disruption of the control of normal circadian rhythm, thus benefiting the survival of glioma cells. Differential expression of circadian clock genes in glioma and non-glioma cells may provide a molecular basis for the chemotherapy of gliomas.

• Animal cells and systems
• /
• v.9 no.2
• /
• pp.79-85
• /
• 2005

Oxidized abasic residues arise as a major class of DNA damage by a variety of agents involving free radical attack and oxidation of deoxyribose sugar components. 2-deoxyribonolactone (dL) is a C1'-oxidized abasic lesion implicated in DNA strand scission, mutagenesis, and covalent DNA-protein cross-link (DPC). We show here that mammalian cell-free extract give rise to stable DPC formation that is specifically mediated by dL residue. When a duplex DNA containing dL at the site-specific position was incubated with cell-free extracts of Po ${\beta}-proficient$ and -deficient mouse embryonic fibroblast cells, the formation of major dL-mediated DPC was dependent on the presence of DNA polymerase (Pol) ${\beta}$. Formation of dL-specific DPC was also observed with histones and FEN1 nuclease, although the reactivity in forming dL-mediated DPC was significantly higher with Pol ${\beta}$ than with histones or FEN1. DNA repair assay with a defined DPC revealed that the dL lesion once cross-linked with Pol ${\beta}$ was resistant to nucleotide excision repair activity of cell-free extract. Analysis of nucleotide excision repair utilizing a model DNA substrate containing a (6-4) photoproduct suggested that excision process for DPC was inhibited because of DNA single-strand incision at 5' of the lesion. Consequently DPC mediated by dL lesion may not be readily repaired by DNA excision repair pathway but instead function as unusual DNA damage causing a prolonged DNA strand break and trapping of the major base excision repair enzyme.

• Journal of the Korean Institute of Oriental Medical Informatics
• /
• v.18 no.1
• /
• pp.78-115
• /
• 2012

Pulse diagnosis has been highly valued in many East Asian medical books, since "Huangdaineijing(黃帝內經)" published. Gigoo-inyoung pulse method(氣口人迎脈法) is one of the four pulse diagnostic methods originally written in"Huangdaineijing(黃帝內經)". At the late 3rd century, "Maijing(脈經)" redefined the positions of Gigoo-inyoung pulse(氣口人迎脈). According to "Maijing(脈經)", both spots were 1fen(分) in front of guan(關) but Gigoo on the right hand and Inyoung on the left hand. At the middle of 13th century, "Maekgyolejijangbyungsikdoseol(脈訣指掌病式圖說)" had detailed view points about the manner of reading Gigoo-inyoung pulse. "Maekgyolejijangbyungsikdoseol(脈訣指掌病式圖說)" explained clearly the position of 1fen(分) in front of guan(關) by sketch of wrist. The location indicated the border of cun(寸) and guan(關). It also explained clearly corresponding certain cun-guan-chi(寸關尺) with Gigoo-inyoung pulse by sketch. "Euihackipmoon(醫學入門)" is a comprehensive book on medicine written by Lee-Cheon(李梴) in Ming(明) dynasty. In this book, pulse diagnosis is the most emphasized method among the four techniques of diagnosis(四診). Furthermore, it introduces detailed information about Gigoo-inyoung pulse method(氣口人迎脈法) in "Gigoo-inyoungmaekgyole(氣口人迎脈訣)". Lee-Cheon concured in the theory of "Yeongruegumbang(永類鈐方)" and "Maekgyolejijangbyungsikdoseol(脈訣指掌病式圖說)" and put a short essay about Gigoo-inyoung pulse named "Gigoo-inyoungmaekgyole(氣口人迎脈訣)" quoted from the two books. Therefore, throughout many chapters of "Euihackipmoon(醫學入門)", Gigoo-inyoung pulse is used in order to distinguish internal damage(內傷) and external contraction(外感). "Maekgyolejijangbyungsikdoseol(脈訣指掌病式圖說)" and "Euihackipmoon(醫學入門)" understood Gigoo-inyoung pulse commonly by corresponding certain cun-guan-chi(寸關尺) with Gigoo-inyoung pulse. Therefrom, Gigoo-inyoung pulse can distinguish seven emotions(七情) of internal damage(內傷) or six excesses(六淫) of external contraction(外感).

• Mass Spectrometry Letters
• /
• v.8 no.1
• /
• pp.8-13
• /
• 2017

Analysis of quinidine for fish tissues using single drop microextraction (SDME) coupled with atmospheric pressure matrix assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) are reported. Optimization conditions; such as extraction solvent, extraction time, pH of the aqueous solution, salt additions (NaCl), stirring rate, matrix type and concentration are investigated. Linear dynamic range (${\mu}M$), limit of detection, relative recovery%, and enrichment factor are 0.08-9.2, 0.05, $94.8{\pm}3.1-98.5{\pm}3.3%$, $4.34{\pm}0.28-4.40{\pm}0.30$, respectively. SDME-AP-MALDI-MS shows good intraday and interday reproducibility.

• Mass Spectrometry Letters
• /
• v.6 no.2
• /
• pp.43-47
• /
• 2015

Detection of mefenamic acid (M, non-steroidal anti-inflammatory drug, NSAIDs) and its metallodrug was investigated using electrospray ionization mass spectrometry (ESI-MS) and fluorescence spectroscopy. ESI-MS data (500 µL, 1×10-3 M) revealed high detection sensitivity for the drug and metallodrug. ESI-MS spectra revealed peaks at 242, 580, and 777 Da corresponding to [M+H]+, [63Cu(M-H)2(H2O)2+H]+, and [56Fe(M-H)3+H]+, respectively. The metal:mefenamic ratios of ESIMS spectra are in complete agreement with the fluorescence spectroscopy results (1:2 for Cu(II) and 1:3 for Fe(III)). ESI is a soft ionization technique that can be used on labile metallo-mefenamic acids and is promising for the detection of these species in environmental samples and biological fluids.