• Asian-Australasian Journal of Animal Sciences
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• 제17권9호
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• pp.1223-1226
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• 2004

Previously candidate gene approach revealed estrogen receptor (ESR) locus was associated with increased litter size. In this study, PvuII polymorphisms of ESR gene was detected by PCR-RFLP, and ESR locus was evaluated for its association with reproductive tracts components in the Large $White{\times}Meishan$ ($LW{\times}M$) F2 offspring. Ninety seven gilts with reproductive tracts components records and 136 offspring with performance traits records were genotyped and the results were used to estimate allele substitution effects. The results showed that two alleles (A and B) were identified, and 121 bp fragments were observed for the AA genotype and 65 bp and 56 bp fragments for the BB genotype; the length of uterine body (LUB) of BB gilts were significantly shorter than AA gilts', the additive effect was -1.762 cm; the uterine weight (UW) of AB gilts were significantly lighter than AA gilts' with the additive effect -18.058 g; no significant associations of ESR alleles with ovulation rate (OR), length of uterine horn (LUH), length of uterine cervix (LUC), weight of two ovaries (OW), volume of uterine lumen (VUL), length of oviduct (LO) were observed. BB genotypes gilts need significantly less days to 100 kg ($D_{100kg}$) than AA genotypes (p<0.01), the additive effect was per copy of B allele. Allele B is also favorable for average daily gain (ADG), with additive effect 0.015 kg/d (p<0.05). There was no difference between genotypes for backfat thickness at the 13th rib (SF13), loin meat height (ELMH), and loin meat percentage was estimated (ELMP), individual birth weight (IBW) and teat number (TN).

• Reproductive and Developmental Biology
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• 제36권2호
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• pp.121-131
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• 2012

After spermatogenesis, spermatozoa come in contact with fluids in the epididymis where they mature. During ejaculation, spermatozoa are mixed with secretions from prostate gland, vesicular glands, and bulbourethral glands. During natural mating, seminal plasma is deposited in the female reproductive tract eliciting various physiological and immunological responses. With the advances in proteomics, the components of seminal plasma have been identified and the information may be valuable in identifying markers for fertility. Components of seminal plasma that affect fertility have been discovered and the mechanism of action of these factors has been determined. The objective of this study was to determine the specific seminal plasma proteins from Korean native cattle, Hanwoo, and Korean native brindle cattle (KNBC) with the long term goal of improving fertilization rate. After SDS-PAGE and 2-dimensional gel electrophoresis, proteins were identified by Q-ToF analysis. They include plasma serine protease inhibitor precursor and platelet-activating factor acetylhydrolase after SDS-PAGE. Number and density of the spots in 2-dimensional gels were higher in KNBC than Hanwoo. Proteins identified from the paired spots of both breeds include chain A, bull seminal plasma PDC-109 Fibronectin Type II module, BSP-30 kDa precursor, and Spermadhesin Z13 or its precursor. Interestingly, some proteins were identified from multiple spots. The functional differences of these diverse forms of the proteins may require further studies. With their previously reported roles in sperm capacitation by these proteins, the studies on the mechanism of action, ligand interaction and the variation in the genome may help improving fertility in cattle.

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.632-637
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• 2006

Oviduct-specific glycoprotein 1 (OVGP1) is implicated in playing a role in fertilization and early embryo development. In this study, we have obtained the sequence of intron 9 of OVGP1 gene in swine. Comparative sequencing of Meishan (a native Chinese breed) and Large White pig breeds revealed an A/T substitution at position 943. A PCR-EcoRI-RFLP assay was developed to detect this mutation. Polymorphism analysis in Qingping animals showed that pigs with BB genotype had lower number of piglets born alive (NBA) in multiple parities than pigs with AA (p<0.05) and AB genotype (p<0.01). In Large $White{\times}Meishan$ ($LW{\times}M$) $F_2$ offspring, the weight of both ovaries (OW) of the BB genotype was significantly lighter than that of AB (p = 0.05) and AA (p<0.01) genotypes. Analysis of the data also revealed that the mutation locus affected these two traits mostly by additive effects. These studies indicated that the polymorphism was associated with NBA and OW in two distinct populations and further investigations in more purebreds or crossbreds are needed to confirm these results.

• Applied Microscopy
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• 제29권2호
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• pp.241-253
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• 1999

북부 온대지방에 서식하는 박쥐는 그 대부분이 동면을하며, 그 생식유형은 특이하여 가을에 교미하여 사정된 정자는 자성생식도내에 긴 동면기간 동안 저장되어 있는 것으로 알려져 있다(예: 한국큰관박쥐). 환언하면 저장되어 있던 정자는 이듬해 봄에 배란되는 난자와 수정하여 초여름에 출산하게 된다. 본 연구는 월동하는 정자 두부 Plasma membrane의 성분과 활성 등 형태적 및 기능적 특성을 관찰 분석함으로써, 인공임신율을 높일 수 있는 보다 효과적인 한냉온도 정자저장방법을 모색하기 위하여 시도되었다. 동면하는 한국큰관박쥐와 햄스타 및 비동면동물(마우스)의 정자 Plasma membrane이, 실온과 한냉온도(박쥐-동면온도)에서, 생존율, 첨체반응을, 단백질분포, 능동수송에 관여되는 효소$(Na^+-K^+-ATPase)$의 활성 및 주사전자현미경적 조직화학상을 분석하였다. 본 연구에서 얻은 실험결과에 의거하면, 동면동물인 한국큰관박쥐와 햄스타 및 비동면동물인 마우스 정자의 생존율과 첨체반응율은, 실온과 한냉(박쥐-동면)온도간에 의의있는 차이를 발견할 수 없었고, 단백질 분포와 $Na^+-K^+-ATPase$ 효소활성 및 주사전자현미경적 조직화학적 소견에 있어서도 두 온도간에 의의있는 차이는 없었다. 이와 같이 실온과 한냉온도 간에 형태적 기능적 차이가 없다고 하는 사실은, 동면동물이든 비동면동물이든 Spermatozoa plasma membrane이 동면중 또는 동면 이후에 안정적인 수정을 보장해 주는 형태적 및 기능적 보호구조임을 시사해 준다.