• Asian-Australasian Journal of Animal Sciences
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• 제9권5호
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• pp.583-590
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• 1996

The purpose of this study was to evaluate some factors in the bovine embryonic development from one-cell to blastocyst using modified synthetic oviduct fluid medium (mSOFM), after maturation and in vitro fertilization of the oocytes. The embryonic development to the blastocyst stage was assessed at 7-10 days after in vitro fertilization, and the total cells in the blastocysts were counted by staining nuclei with fluorochrome. Some commercial calf sera (CS) and a superovulated cow serum had different effects on the embryonic development to the blastocyst stage (8.6-21.4%), dependent upon their product lots, although the development might not be affected at least by serum progesterone levels. ${\beta}$-Mercaptoethanol (${\beta}$-ME) supplemented into mSOFM was effective to the embryonic development (27.8%), as well as the co-culture system with cumulus cells (19.5%). In a serum- and feeder cell-free culture using mSOFM containing several growth factors and ${\beta}$-ME instead of CS plus co-cultured cumulus cells, bovine serum albumin (BSA, fraction V), but not polyvinyl alcohol (PVA), was highly effective in embryonic development to the blastocyst stage, almost comparable to CS in the serum-contained culture (CS, BSA and PVA; 27.8, 19.5 and 5.7%, respectively). However, fatty acid free BSA rather reduced the number of developed blastocysts, compared with fraction V BSA (7.3 vs 29.4%). In the serum- and feeder cell-free culture, supplement of glucose to the medium (final 2.0 mM) stimulated the cell proliferation of developing embryos 120 hr after in vitro fertilization. These results indicated that a serum-free medium supplemented with ${\beta}$-ME could successfully support the development of bovine one-cell embryos to the blastocyst stage. Moreover, supplement of glucose and fatty acids to the medium might support preferably the development and cell proliferation of embryos.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2001년도 제41차 추계학술대회
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• pp.103-103
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• 2001

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2002년도 제42차 춘계학술대회
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• pp.88-89
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• 2002

• Journal of Microbiology and Biotechnology
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• 제32권1호
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• pp.126-140
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• 2022

Stem cells can be applied usefully in basic research and clinical field due to their differentiation and self-renewal capacity. The aim of this study was to establish an effective novel therapeutic cellular source and create its molecular expression profile map to elucidate the possible therapeutic mechanism and signaling pathway. We successfully obtained a mesenchymal stem cell population from human embryonic stem cells (hESCs) cultured on chemically defined feeder-free conditions and treated with connective tissue growth factor (CTGF) and performed the expressive proteomic approach to elucidate the molecular basis. We further selected 12 differentially expressed proteins in CTGF-induced hESC-derived mesenchymal stem cells (C-hESC-MSCs), which were found to be involved in the metabolic process, immune response, cell signaling, and cell proliferation, as compared to bone marrow derived-MSCs(BM-MSCs). Moreover, these up-regulated proteins were potentially related to the Wnt/β-catenin pathway. These results suggest that C-hESC-MSCs are a highly proliferative cell population, which can interact with the Wnt/β-catenin signaling pathway; thus, due to the upregulated cell survival ability or downregulated apoptosis effects of C-hESC-MSCs, these can be used as an unlimited cellular source in the cell therapy field for a higher therapeutic potential. Overall, the study provided valuable insights into the molecular functioning of hESC derivatives as a valuable cellular source.

• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1717-1725
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• 2000

The objective was to determine the relationship between feed intake levels and patterns, and growth performance and body composition of barrows and gilts using automatic feed intake recording equipment (F.I.R.E.). This system records the time of visits to the feeder and the duration and size of meals for individual animals housed in groups. Ninety-six crossbred pigs were grown from $33.4{\pm}0.51$ to $109.7{\pm}1.39kg$ live weight over a 13-week period. Eight mixed-sex groups of 12 pigs were used and 4 dietary treatments were compared giving 2 pens per treatment. The dietary treatments consisted of corn-soybean meal diets with differing protein levels which ranged from 14.7% to 19% between 30 to 55 kg, from 13.3% to 16.9% between 56 and 85 kg, and from 12.3% to 16.8% for the remainder of the study. Animals were ultrasonically scanned to measure loin-eye area and backfat thickness to estimate carcass fat-free lean content at the beginning and end of the study. Barrows had higher daily feed intake than gilts (2.67 vs. 2.46 kg resp. p<0.05) which was the result of a longer feeder occupation time per visit (4.77 vs. 4.54 min, resp. p<0.05), higher feed consumption rates (30.4 vs. 29.0 g/min, resp. p<0.05), and higher feed intakes per visit (136.9 vs. 126.8 g, resp. p<0.01). Gilts had less backfat and greater loin-eye area than barrows (p<0.05). Diet had no significant effect on growth performance and had limited impact on feeding patterns. Body weight showed high correlations with ADG (r=0.74), feed intake per visit (r=0.51) and feed consumption rate (r=0.69). Positive correlation were also found between daily feed intake and feed intake per visit (r=0.45), feeder occupation time per day (r=0.56), and feed consumption rate (r=0.55), and between daily feed intake and backfat thickness (r=0.32) and feed consumption rate and loin-eye area (r=0.32). There were negative correlations between number of feeder visit per day and daily feed intake (r=-0.54), and between feed intake per visit and number of feeder visits per day (r=-0.43). However, correlations between feed intake traits and carcass traits were generally low. Visits to the feeder were greatest during the morning (0700 to 1100 h) and lowest during the evening and nighttime. These results highlight limited variation among the sexes in feeding patterns and suggest important relationships between feeding behavior and feed intake.

• Journal of Electrical Engineering and Technology
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• 제13권2호
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• pp.669-676
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• 2018

Transmission system has been well studied since long time and power system techniques of distribution system are more or less derived from transmission system. However, unlike transmission systems, many practical issues are encountered in the distribution system. Considerable amount of error is observed in voltage obtained from the Feeder Remote Terminal Units (FRTUs) measured by the pole mounted PTs along the distribution feeder. Load uncertainty is also an issue in distribution system. Further, penetration of Distributed Generators (DGs) creates voltage variations in the system. Hybrid radial/ loop distribution system also make it complicated to handle distribution system. How these constraints to be handled under Distribution Automation (DAS) environment in order to obtain error free voltage is described in this paper and therefore, a new approach of voltage error correction technique has been proposed. The proposed technique utilizes reliable data from substation and the FRTUs installed in DAS. The proposed technique adopts an iterative process for voltage error correction. It has been tested and proved accurate not only for conventional radial systems but also for loop distribution systems.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 학술대회지
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• pp.850-852
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• 2004

The usable output of glassware formation production line depends, among other factors, on the weight and shape of a lump of hot molten glass called a gob. In this paper, an automated image processing system for monitoring gob weight and shape on a production line is proposed. Present techniques often rely on manual weighing of gobs on a sample basis by manually controlling the gob feeder. The proposed automated image monitoring system checks the weight and shape of every free-falling gob before it reaches the mould. In this system, the estimated weight of the gob is sufficiently close to its actual weight.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 International Symposium on GPS/GNSS Vol.1
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• pp.441-446
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• 2006

The world economy has came into unlimited competition with globalization since 1990. Opening markets to the world is in progress through the expansion of world free trade and internationalization of multinational enterprises. In the maritime transportation for world trade, shipping companies pursue "Hub & Spoke" strategy so a port which is not able to be located as a hub port is degraded as a feeder port. To attract shipping companies, it is necessary for existing ports to provide differentiated service. This paper devises marketing strategies for a competitive port after evaluating relation among the factors affecting port selection when a shipping company chooses a port of call. On the basis of determinants derived from existing researches, we study the relation as well as importance among the factors of port selection.

• Journal of Astronomy and Space Sciences
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• 제37권2호
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• pp.117-129
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• 2020

In a Satellite communication system, a link budget analysis is the detailed investigation of signal gains and losses moving through a channel from a sender to receiver. It inspects the fading of passed on data signal waves due to the process of spreading or propagation, including transmitter and receiver antenna gains, feeder cables, and related losses. The extent of the proposed tool is to make an effective, efficient, and user-friendly approach to calculate link budget analysis. It is also related to the satellite communication correlation framework by building up a graphical interface link analysis tool utilizing STK^® software with the interface of C# programming. It provides better kinds of graphical display techniques, exporting and importing data files, printing link information, access data, azimuth-elevation-range (AER), and simulation is also possible at once. The components of the link budget analysis tool include transmitter gain, effective isotropic radiated power (EIRP), free space loss, propagation loss, frequency Doppler shift, flux density, link margin, elevation plot, etc. This tool can be useful for amateur users (e.g., CubeSat developers in the universities) or nanosat developers who may not know about the RF communication system of the satellite and the orbital mechanics (e.g., orbit propagators) principle used in the satellite link analysis.

• Fisheries and Aquatic Sciences
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• 제4권3호
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• pp.130-137
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• 2001

In the present study, the locomotor and feeding activities of single red sea bream, Pagrus major were simultaneously investigated to examine the existence of such dual behaviour. Seven red sea bream of 13cm body length on average were placed individually in 35L tanks equipped with an infrared sensor and a newly developed demand-feeding device. Fish were exposed to a light: dark 12: 12h cycle and constant darkness (DD) to study endogenous rhythmicity. Under LD 12: 12 h, the daily pattern of behaviour differed between individual fish; some red sea bream were diurnal and others were nocturnal. Futhermore, some of them displayed an extraordinary flexibility in phasing because they were dark active but light feeding, and vice versa. Under DD, red sea bream showed free-running rhythms for locomotor activity and feeding. These results indicate that the type of phasing of locomotor activity did not necessarily decide the feeding phase; much of this is explained by the fact that red sea bream were demand-fed. Flexibility in phasing and a certain degree of independence between locomotor and feeding activities could be seen as an adaptative response of the highly adaptable circadian rhythms of fish.