• 환경생물
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• 제39권4호
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• pp.415-422
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• 2021

면화는 중요한 섬유 작물로 종자는 가축의 사료로 사용된다. 작물 생명공학은 농업 분야에서 농업적 형질과 질을 향상시키기 위해 활용되어져 왔다. 국내 식품, 사료, 가공 제품에 유전자변형(LM) 면화의 사용이 증가함에 따라 환경으로의 LM 면화의 비의도적 유출 또한 증가하고 있다. LMO 모니터링 사업에서 수집된 LM 면화를 검정하기 위하여 국내 수입 승인된 LM 면화의 검출법 개발이 필요하다. 본 연구에서는 LM 면화 MON757, MON88792, COT67B, GHB811 4종을 대상으로 동시검출법을 개발하였다. 이벤트에 대한 유전 정보는 유럽 JRC와 농림축산검역본부에서 확보하였다. LM 면화의 동시검출법 개발을 위해 이벤트 특이적인 프라이머를 설계하였으며 특이적인 증폭을 확인하였다. 특이도 검정, 무작위 표준물질 혼합물 분석, 검출한계 분석을 통하여 동시검출법의 정확도와 특이도를 검증하였다. 그 결과 본 동시검출법은 각각의 이벤트를 검출할 수 있으며 LM 표준물질을 활용하여 특이도를 검정하였다. 또한 무작위 표준물질 조합도 정확하게 검출할 수 있다. 검출한계 분석에서는 25 ng의 미량의 주형 DNA로 단회 분석으로 검출이 가능하다. 결론적으로 4종의 LM 면화 동시검출법을 개발하였으며 LM 면화 자생체 분석에 활용될 것으로 사료된다.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2010년도 추계학술대회
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• pp.277-281
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• 2010

본 연구에서는 사료분진의 폭발 특성을 연구하여 분진에 의한 폭발사고 위험을 감소시키고 방지대책에 필요한 기초자료를 제공하기 위해 Hartman1)식 분진폭발장치를 사용하여 다양한 사료를 실험하였다. 실험결과로 사료농도가 폭발확률에 미치는 영향은 농도가 높을수록, 사료분진의 입경이 작을수록 폭발확률이 커지고 분진농도가 증가할수록 폭발압력이 증가하였으나 일정농도를 넘어서면 오히려 폭발압력이 감소하는 경향을 보였으며 불활성물질을 첨가할 경우에는 10%이상 첨가할 경우에 폭발억제 효과를 보였다.

• 한국수산과학회지
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• 제47권4호
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• pp.341-346
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• 2014

Transmissible spongiform encephalopathies (TSEs), also termed prion diseases, are a threat to food safety and to human and animal health. Variant Creutzfeldt-Jakob disease (vCJD) in humans is caused by the consumption of meat contaminated with bovine spongiform encephalopathy (BSE, mad cow disease). The BSE epidemic in the United Kingdom was shown to be related with the extensive use of BSE-contaminated meat-and-bone meal (MBM) and bovine offal. Many countries worldwide use MBM, as well as meat from cows, for aquaculture feed. This raises concerns about the safety of farmed fish, a major protein source for humans. The present work reviews recent studies on fish prion protein and the transmissibility of mammalian prion agents to fish, providing insights into the future direction of fish prion research.

• 한국식품위생안전성학회지
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• 제14권2호
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• pp.129-133
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• 1999

This study was designed to observe the effects of T-2 toxin on total cholesterol and lipid concentration in rat serum. T-2 toxin is a secondary metabolite produced by Fusarium sp. which is often found on agricultural products including cereals, and it is a causal material of liver injuries in cattle and humans. When we fed rats with standard diet treated with T-2 toxin, the body weight and feed consumption of rats treated T-2 toxin were decreased. As the results of lipid analysis, the concentrations of total cholesterol and free cholesterol in serum of treated rats were increased compared to non-fed control group, On the other hand, the levels of triglyceride and phospholipid in the serum of T-2 toxin treated experimental groups were declined. In conclusion, T-2 toxin largely influenced on the total cholesterol and lipid levels in rat serum.

• 한국안전학회지
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• 제16권3호
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• pp.117-124
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• 2001

This paper introduces m analysis framework and procedure for the support of the cognitive error analysis of emergency tasks in nuclear poler plants. The framework provides a new perspective in the utilization of influencing factors into error prediction. The framework can be characterized by two features. First, influencing factors that affect the occurrence of human error me classified into three groups, i.e., task characteristic factors(TCF), situation factors(SF), and performance assisting factors(PAF). This classification aims to support error prediction from the viewpoint of assessing the adequacy of PAF under given TCF and SF. Second, the assessment of influencing factors is made by each cognitive function. Through this, influencing factors assessment and error prediction can be made in an integrative way according to each cognitive function. In addition, it helps analysts identify vulnerable cognitive functions and error factors, and obtain specific nor reduction strategies. The proposed framework was applied to the error analysis of the bleed and feed operation of nuclear emergency tasks.

• 대한안전경영과학회지
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• 제4권2호
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• pp.209-221
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• 2002

Machinability improvement by the use of liquid nitrogen in cryogenic machining has been reported in various studies. This has been mostly attributed to the cooling effect of liquid nitrogen. However, No study has been found in discussion on whether liquid nitrogen possesses lubrication effect in cryogenic cutting. In machining tests, cryogenic machining reduced the force component in the feed direction, indicating that the chip slides on the tool rake face with lower friction. This study also found that the effectiveness of LN2 lubrication depends on the approach how LN2 is applied regarding cutting forces related.

• Nuclear Engineering and Technology
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• 제56권5호
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• pp.1603-1618
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• 2024

Emergency operation plans are indispensable elements for effective process safety management especially when unanticipated events occur under extreme situations. In the paper, a synthesis framework is proposed for the integration success path planning and performance analysis. Within the synthesis framework, success path planning is implemented through flow-directed signal tracing, renaming and reconstruction from a complete collection of Minimal Path Sets (MPSs) that are obtained using graph traversal search on GO-FLOW model diagram. The performance of success paths is then evaluated and prioritized according to the task complexity and probability calculation of MPSs for optimum action plans identification. Finally, an Auxiliary Feed Water System of Pressurized Water Reactor (PWR-AFWS) is taken as an example system to demonstrate the flow-directed MPSs search method for success path planning and performance analysis. It is concluded that the synthesis framework is capable of providing procedural guidance for emergency response and safety management with optimal success path planning under extreme situations.

• 한국안전학회지
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• 제31권5호
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• pp.187-194
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• 2016

A single fire event within a fire area can cause multiple initiating events considered in internal events probabilistic safety assessment (PSA). For an example, a fire event in turbine building fire area can cause a loss of the main feed-water and loss of off-site power initiating events. This fire initiating event could result in special plant responses beyond the scope of the internal events PSA model. One approach to address a fire initiating event is to develop a specific fire event tree. However, the development of a specific fire event tree is difficult since the number of fire event trees may be several hundreds or more. Thus, internal fire events PSA model has been generally constructed by modifications of the pre-developed internal events PSA model. New accident sequence logics not covered in the internal events PSA model are separately developed to incorporate them into the fire PSA model. Recently, many fire PSA models have fire induced initiating event fault trees not shown in an internal event PSA model. Up to now, there has been no analytical comparative study on the constructions of fire events PSA model using internal events PSA model with and without fault trees of initiating events. In this study, the changing process of internal events PSA model to fire events PSA model is analytically presented and discussed.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1219-1225
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• 2022

Microorganisms involved in food fermentation not only improve the aroma and taste of the food, but also enhance its preservation. Thus, they are added as starter cultures to boost the final product quality of commercial fermented foods. Although these microorganisms originate from fermented foods and have a long history of consumption, the European Union recently applied the concept of Qualified presumption of Safety (QPS), which is a safety evaluation system for microorganisms used in food or feed in Europe. The QPS system is a species-level safety system and shares results with the European Novel Food System, a strain-level safety evaluation system. In the United States, microorganisms added to fermented foods are considered as food additives or Generally Recognized as Safe substance. In Korea, food microbe lists are presented at the species level. Moreover, the nation has established a strain-oriented evaluation system that applies temporary safety evaluation methods for food raw materials as well as new raw materials. However, when it comes to microorganisms isolated from traditional fermented foods and other fermented food products, there is no definition of the term "species," and there is a lack of an evaluation system at the species level. Therefore, such an evaluation system for microbial species used in Korean fermented foods is necessary.

• 한국산업보건학회지
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• 제10권2호
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• pp.109-123
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• 2000

Inhaled halogen compound was examined through micronucleus tests and toxicity tests using SD (Sprague-Dawley) rats with repeated dosages for six hours a day, five days a week, during four weeks. In four-week repeated exposure, no specific sign caused by the $CF_3I$ compound was observed on the clinical symptoms, body weight variation, feed consumption, and urinalysis data in the testing groups with reference to the control group. In hematological and biochemical blood tests of the testing groups, the significant, but in their normal ranges, value dependencies of glucose, aspartate aminotransferase, alanine aminotransferase and etc., on the halogen carbohydride concentration was observed. In histo-pathological tests, no specific lesion or concentration dependent change due to the $CF_3I$ compound dosage was observed in both sexes of the female and male in the tested animals. But, micronucleus tests on marrow cells extracted from the tested animals which were repeatedly exposed in the $CF_3I$ compound during four weeks, the frequencies of micronuclei were significantly increased dose-dependently compared to the control groups.