• Journal of Food Hygiene and Safety
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• v.14 no.1
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• pp.1-8
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• 1999

There were few data for the distribution of the indicator organisms in the commercial plant foods, and for the normal flora and for the foodborne agents within the country. First of all it must be investigated the distribution of the indicator organisms. And also it is very important to prepare the sanitation criteria for the plant foods through the microbiological examination and the investigation of tendency to change of the indicator organisms according to the storage temperature and period. The average number of total viable counts for grains was 2.9$\times$105/g, psychrophilic bacteria 2.9$\times$105/g, heterotrophic bacteria 3.1$\times$105/g, heat-resistant bacteria 2.1$\times$103/g, Pseudomonas aeruginosa 23/g. That for beans was 6.3$\times$102/g, psychrophile 34/g, heterotroph 1.7$\times$102/g. That for sesames was 1.4$\times$105/g, coliform 350/g, psychrophile 7.4$\times$104/g, heterotroph 5.8$\times$104/g, Pseud. aeruginosa 2.3$\times$103/g. heat-resistant bacteria 150/g. That for potatoes was 2.0$\times$107/g, coliform 5.0$\times$104/g, psychrophile 1.8$\times$107, heterotroph 1.4$\times$107/g, heat-resistant bacteria 3.3$\times$104/, Staphylococcus 2.7$\times$105/g, fecal streptococcus 4.5$\times$103/g, Pseud. aeruginosa 7.0$\times$103/g. That for mushrooms was 1.2$\times$108/g, psychrophile 9.4$\times$107/g, heterotroph 1.0$\times$109/g, heat-resistant bacteria 1.6$\times$105/g, Pseud. aeruginosa 1.3$\times$103/g. That for vegetables was 5.9$\times$1011/g, coliform 1.8$\times$106g/, Staphylococcus 1.1$\times$1012/g, heterotroph 8.4$\times$1011/g, heat-resistant bacteria 7.6$\times$106/g, Staphylococcus 1.1$\times$107/g, fecal streptococcus 1.1$\times$104/g, Pseud. aerugniosa 5.2$\times$104/g. That for nuts 3.9$\times$104/g, coliform 3.9$\times$103/g, psychrophile 4.0$\times$104/g, heterotroph 3.2$\times$104/g, heat-resistant bacteria 400/g. In commercial grains and beans, SPC, psychrophile, heterotroph and heat-resistant bacteria stored at 1$0^{\circ}C$, 2$0^{\circ}C$, 3$0^{\circ}C$ were constant. Staphylococcus, coliform, Pseud. aeruginosa were decreased a little n grains, but were not detected in beans. In mushrooms, all indicator organisms were increased as time goes on and were increased rapidly at 2$0^{\circ}C$. In sesames, coliform was not detected at all temperature. psychrophile was increased for 7 days, the others were constant. In potatoes, SPC, psychrophile, heat-resistant bacteria, heterotroph had a tendency to increase and the others were constant. In vegetables, indicator organisms were had a tendency to increase, psychrophile, heterotroph were rapidly increased after 7 days. In nuts, SPC, coliform, psychrophile heterotroph, heat-resistant bacteria, Pseud. aeruginosa were constant, staphylococcus and fecal streptococcus were not detected.

• Parasites, Hosts and Diseases
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• v.54 no.5
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• pp.645-652
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• 2016

Toxocara vitulorum has been rarely reported in yaks at high altitudes and remote areas of Sichuan Province of Tibetan Plateau of China. The current study was designed to investigate the prevalence, associated risk factors, and phylogenetic characteristics of T. vitulorum in yak calves on the Qinghai Tibetan plateau. Fecal samples were collected from 891 yak calves and were examined for the presence of T. vitulorum eggs by the McMaster technique. A multivariable logistic regression model was employed to explore variables potentially associated with exposure to T. vitulorum infection. T. vitulorum specimens were collected from the feces of yaks in Hongyuan of Sichuan Province, China. DNA was extracted from ascaris. After PCR amplification, the sequencing of ND1 gene was carried out and phylogenetic analyses was performed by MEGA 6.0 software. The results showed that 64 (20.1%; 95% CI 15.8-24.9%), 75 (17.2; 13.8-21.1), 29 (40.9; 29.3-53.2), and 5 (7.6; 2.5-16.8) yak calves were detected out to excrete T. vitulorum eggs in yak calve feces in Qinghai, Tibet, Sichuan, and Gansu, respectively. The present study revealed that high infection and mortality by T. vitulorum is wildly spread on the Qinghai Tibetan plateau, China by fecal examination. Geographical origin, ages, and fecal consistencies are the risk factors associated with T. vitulorum prevalence by logistic regression analysis. Molecular detection and phylogenetic analysis of ND1 gene of T. vitulorum indicated that T. vitulorum in the yak calves on the Qinghai Tibetan plateau are homologous to preveiously studies reported.

• Journal of Life Science
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• v.22 no.3
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• pp.324-331
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• 2012

Bacteriological examination of spring water in Jeju City was conducted. A total of 21 spring water samples were collected from January to April, 2010. During the study period, the range of temperature was 0.6 to $15.4^{\circ}C$, and the results of the analyses showed that hydrogen ion concentrations (pH) for spring water were 0.43 to 7.9. Salinity levels for the samples averaged from 3.0 to 1.64%, and levels of water-dissolved oxygen were 1.85 to 6.06 mg/l. The range of total coliforms in spring water samples at 21 stations located in the designated spring water were <1.8->1,600 MPN/100 ml. Furthermore, the range of geometric means of total coliforms was 9.9-151.6 MPN/100 ml, while the range of fecal coliforms in spring water samples at 21 stations located in the designated spring water area was <1.8->1,600 MPN/100 ml. Finally, the range of geometric mean of fecal coliforms was 3.1-151.6 MPN/100 ml. The level of microbial contamination was examined in 21 samples for indications of bacterial contamination such as heterotrophic bacteria, Escherichia coli O157:H7, Salmonella spp., Vibrio parahaemolyticus, Staphylococcus aureus, and Shigella spp. were frequently detected in the spring water. Salmonella spp., Shigella spp., Vibrio parahaemolyticus, and S. aureus were detected in the range of $0-0.5{\times}10^1$, $0-0.1{\times}10^1$, $0-0.1{\times}10^1$, and $0-0.3{\times}10^1$ CFU/ml, respectively, while E. coli O157:H7 was not detected in the examined spring water samples.

• Journal of Veterinary Clinics
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• v.26 no.5
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• pp.463-466
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• 2009

A swine farm located in the Kyungpook province (designated as farm D that have been suffering from PED for several years was selected to study the etiology and the outbreak pattern of PED by clinical and laboratory examinations. Clinical examination indicated that sows exhibited signs of mastitis resulting in an inadequate transfer of lactogenic immunity against PEDV to newborn piglets. Furthermore, serological tests revealed that all sow groups and their piglets had low levels of anti-PEDV antibody. These data suggest that improper vaccination program has been indeed performed in this farm. Remarkably, despite no symptoms of PED in weaners, the presence of PEDV was identified by RT-PCR from fecal samples of weaning piglets, indicating persistent PEDV circulation in the herd. Based on these results, the following basic control schemes were executed for the control of PEDV circulation in the farm; a) A quick removal of affected pigs and disinfection of affected sheds. b) restructuring of vaccination program and employment of consultant. c) prompt treatment of mastitis and removal of poor lactogenic sows, and d) enhancement of biosecurity of farrowing house by acquisition of additional space. We evaluated risk factors and implementation of control measures in two months and were unable to found any case related to PEDV infection. Taken together, our data indicate that the method described above is effective for the control of PED outbreak in farm persistently suffering from PEDV infection.

• Journal of the korean society of oceanography
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• v.34 no.2
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• pp.113-121
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• 1999

Glauconite grains are widely distributed in the outer shelf surficial sandy sediments of the Korean continental shelves. Morphologically, these grains are characterized by ovoidal-shaped pellets with bulbous exterior, greenish color and moderately well polished surface. In thin sections, the glauconite grains are massive and contain numerous impurities composed mainly of quartz, feldspar and mica. The morphologies and microscopic examinations indicate mainly fecal pellet as a parent matierials for glauconite. SEM examination shows that glauconitic smectite crystals grow in pores and along fractures of the host grains, commonly as a boxwork arrangement or rosette clusters of blades. Bulbous external shape, high potassium content and rosette-shaped smectite structure, reflect the evolved stage (more than 10$^4$ years in age) of glauconite authigenesis. Moreover, the association of the glauconite grains with the relict sandy sediments on the outer shelf, impliesthat the glauconite grains are geologically old, or at least have formed under the environmental conditions different from the present shelf. The widespread occurrence of the glauconite grains, therefore, might be primarily related with redeposition of sediments transported from the outer shelf during Holocene transgression.

• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.219-223
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• 1983

Echinostomatid eggs were found from a 21year old man, whose native village is Munkyong-gun, Kyongsangnam-do during fecal examination in October 1983. The eggs were detected again one week later concommitantly with the eggs of Clonorchis sinensis. He had no subjective symptoms related with these fluke infections. He was treated with praziquantel ($Distocide^{\circledR}$) 20 mg/kg in single dose and purgated with magnesium salt. One echinostomatid fluke and 4 adults of C. sinensis were collected from the diarrheal stools. After morphological observation, the echinostomatid fluke was identified as Echinostoma hortense Asada, 1926. This is the first record on human E. hortense infection in Korea.

• Journal of Korean Ophthalmic Optics Society
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• v.9 no.2
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• pp.447-453
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• 2004

The prevalence of Toxocara canis eggs in the public places in the city of Gwangju were studied through examination of 800 soil and 123 fecal specimens. Toxocara canis eggs were found in 15% of soil samples. In 25% parks and 17% residential quarters, these eggs were detected with higher contamination rate than other places. The soil contamination rate of playgrounds in spring and autumn was higher than that of summer and winter, and the variation range of the rate was high in parks and residential quarters. The contamination rate of eggs in 123 samples of dogs' feces was 31%, it was higher than that of soil samples(21%).

• Environmental Engineering Research
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• v.19 no.1
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• pp.67-73
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• 2014

In India, disposal of vegetable market waste along with municipal solid waste in landfills or dumpsites is creating much nuisance in terms of odor nuisance, leachate production, and greenhouse gas emission into the atmosphere. Therefore, vegetable waste with high biodegradable and nutrient content is composted in a 550-L batch scale rotary drum composter to study the degradation process and its compost properties for its potential reuse as high quality compost. A total 150 kg of working volume was fixed for composting studies with two different ratios, trial A (6:3:1) of C/N 24 and trial B (8:1:1) of C/N 30, respectively. A maximum of $63.5^{\circ}C$ and $61.2^{\circ}C$ was observed in trials A and B; an average of $55^{\circ}C$ for more than 5 days, which helped in the degradation of organic matter and reduction of total and fecal coliform. The temperature dropped suddenly after the thermophilic stage in trial B, and leachate was observed due to insufficient amount of bulking agent. Mesophilic bacteria dominated during the initial stages of composting, and reduced considerably during the thermophilic stage. During the thermophilic stage, the rise in spore-forming organisms, including spore-forming bacteria, fungi, actinomycetes and streptomycetes, increased and these were predominant until the end of the composting process. By examination, it was observed that moisture and leachate production had adverse effects on the compost parameters with higher loss of micronutrients and heavy metals.

• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.469-473
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• 1999

A 120 kg, 3-year-old, male ostrich (Struthio camelus) was submitted to the veterinary teaching hospital, Chonbuk national university, for chronic weight loss, anorexia, small hard fecal pellets, and lethargy. Illness was first noticed approximately 2 weeks before submission. Although the bird was able to stand, he was extremely weak and cachectic. Blood examination indicated that the bird was dehydration (PCV: 55%). Fluoroscopic and lateral abdominal radio-graphic view indicated impaction of distended proventriculus with bigger rocks than normal. Anesthesia was induced with ketamine (10 mg/kg, IV)-xylazine (1 mg/kg, IV) combination and maintained with ketamine (10 mg/kg, IV) at intervals of 8 to 15 minutes during the entire procedure. After induction of general anesthesia, the bird was positioned in dorsal recumbency. The skin was incised approximately 15cm caudal to the caudal margin of the sternum and extended 20 to 25 cm caudally. After the proventriculotomy was peformed, the impacted material that weighed 3kg contained rocks in 4 to 6cm diameter was removed. The incision of proventriculus was closed in 2 layers. Cephalothin sodium (30 mg/kg, IV, q12hrs) and dexamethazone (2 mg/kg, IM, q24hrs) was administered before and after 5 days of the operation. The bird was eating well and defecating normal feces after 3 weeks of the surgery

• Journal of Veterinary Clinics
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• v.35 no.5
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• pp.240-242
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• 2018

A 1-year-old, female Bengal tiger (Panthera tigris tigris) presented signs of weight loss and dark browncolored diarrhea. On fecal examination, numerous intact and broken red blood cells were found, but both parasites and inflammatory signs were absent. No significant findings were seen in serum biochemistry profiles, including electrolytes, with negative feline pancreatic lipase immunoreactivity (fPLI). Diagnostic kits using feces or peripheral blood were negative for feline parvovirus, feline coronavirus, feline immunodeficiency virus, and feline leukemia virus. Based on the result of feline trypsin-like immunoreactivity (fTLI) concentration ($4.6{\mu}g/L$), the tiger was provisionally diagnosed to have exocrine pancreatic insufficiency (EPI). After this diagnosis, pancreatic enzymes were prescribed. The feces of the tiger returned to normal form and her weight was increasing. EPI is uncommon and not described extensively in Felidae, including domestic cats. Feline EPI is associated with a variety of non-specific signs and it should be considered in the differential diagnosis of cases presenting with weight loss, diarrhea, and other gastrointestinal signs. In this case, the patient was strongly suspected to have EPI based on the very low fTLI concentration, though the concentration of fTLI in tigers has not yet been determined. This is the first report to present a suspected EPI case in Bengal tigers.