• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.8
• /
• pp.1137-1146
• /
• 2000

This study was carried out to establish an optimum number of phase feeding regimen which enable to reduce nutrients excretion without affecting growth performance and to investigate the effects of different feeding regimens on growth performance, nutrients excretion and carcass characteristics in finishing pigs. A total of 120 finishing pigs (an average initial body weight of 54.3 kg) were assigned to the feeding trial and 12 pigs were assigned to the metabolic trial. Treatments included one phase (54 to 104 kg), two phase (54 to 80 and 80 to 104 kg), three phase (54 to 70, 70 to 90 and 90 to 104 kg) and four phase (54 to 65, 65 to 80, 80 to 95, 95 to 104 kg) feeding regimens. Experimental diets were formulated to contain 16% crude protein for one phase feeding regimen, 16% and 12% crude protein for two phase feeding regimen, 16%, 14% and 12% crude protein for three phase feeding regimen, and 16%, 14.7%, 13.4% and 12% crude protein for four phase feeding regimen, respectively. Although there were no significant differences in any criteria measured during the entire experimental period, pigs reared in three phase feeding regimen grew slightly faster than those reared in other feeding regimens and showed a tendency to increase ADFI during the whole experimental period. The metabolic trial indicated that there were no significant differences in DM (dry matter), CP (crude protein) and P (phosphorus) digestibilities. However, fecal nutrient excretion except P was significantly influenced by feeding regimens. DM excretion of one phase feeding group was significantly higher than that of three phase feeding group and daily fecal N (nitrogen) excretion of one phase feeding group was higher than that of other phase feeding groups (p<0.05). Three and four phase feeding regimens resulted in 12% lower fecal N and DM excretion than one phase feeding regimen. Blood urea concentrations were lower for pigs reared in two, three and four phase feeding regimens than for those reared in one phase feeding regimen (p<0.05). Three phase feeding regimen for the finishing period showed better carcass grade than one phase feeding regimen, though the difference was not significant. The tenth rib fat thickness of pigs fed on four phase feeding regimen was reduced most and there was a trend that backfat thickness decreased as the number of phases increased. Feed cost per kg weight gain was significantly low in four phase feeding group than one phase feeding group (p<0.05). In summary, it seemed that producers generally oversupply the expensive nutrients for the finishing pigs. High nutrient diets do not always guarantee high growth rate of pigs and cause more unwanted nutrient excretion. It rather seems that meeting nutrient requirements for the each growth phase is more important for the reduction of pollutants and economical pork production.

• Korean Journal of Poultry Science
• /
• v.28 no.1
• /
• pp.27-40
• /
• 2001

This study was conducted to investigate the effects of feeding various Lactobacillus on performance, nutrients digestibility, intestinal microflora, and fecal noxious gas of broiler chicks. One thousand eighty one dat old male broiler chicks were fed into Lactobacillus crispatus avibrd 1(LCB), Lactobacillus reuteri avibro 2(LRB), Lactobacillus crispatus avihen 1(LCH), and Lactobacillus vaginalis avehen 2(LVH) at the level of $10^{4}$ and 10(sup)7cfu/g diet. There were four replicates with thirty chicks per pen. Diets contained ME 3,100, 3,100kcal/kg, and CP 22.0, 20.0% for starting and finishing period, respectively. Weight gain of checks fed Lactobacillus spp. tended to increase from the first week and was higher from 50 to 100g in Lactobacillus treatments than control. Feed intake and feed conversion were not statistically different of all treatments. Dry Matter(DM) digestibility of Lactobacillus treatments was tended to improve compared to that of control, but was not significantly different. Protein and Ca digestibility were also tended to improve in Lactobacillus treatments relative that of control. Lactobacillus treatments showed improved tendency in crude ash and fat compared to those of control, whereas phosphorus digestibility was not consistency. Nutrients digestibilities of bird fed LCH were superior to those of other treatments. It showed significantly higher in Ca and P digestibility than control(P〈0.05). Total Lactobacillus spp. of birds fed various lactobacillus was significantly higher in ileum for five weeks(P〈0.05), but was not different at cecum. Yeast was thought to be not completely attached to intestinal lumen for one week. However, total number of yeast was significantly increased in cecum and ileum from three weeks old chicks(P〈0.05). The number of anaerobes exhibited to tendency the increase in Lantobacillus treatments from one week old of age at both ileum and cecum. Moisture contents of birds fed Lactobacillus spp. was shown from 27 to 30% at five weeks old, whereas it was 38.2% in control. It decreased approximately 25% in Lactobacillus treatments relative to that of control and showed significance between them(P〈0.05). Fecal $NH_{3}$ gas emission was significantly decreased in Lactobacillus treatments and maintained $\frac{1}{3}$to $\frac{1}{2}$ of control(P〈0.05). LCH and LVH treatments were most effective to decrease fecal $NH_{3}$ gas and especiallt impreved ti significantly at the level of the level of 10(sup)7cfu/g diets covpared to that of $10^{4}$ cfu/g diets and control(P〈0.05). In conclusion, feeding Lactobacillus spp. increase performance and nutrients digestibilities of broiler chicks and improve environments of house. Proper supplemental level of Lactobacillus spp. is considered more than $10^{4}$ cfu/g diet.

• Journal of Animal Science and Technology
• /
• v.46 no.6
• /
• pp.981-988
• /
• 2004

An Experiment was conducted to investigate the effects of Natufermen$^{(R)}$;(Aspergillus oryzae) supplementation on the performance, ammonia gas production, microbial population in manure and serum IgG in pigs. Forty eight cross bred(YLD) weanling pigs(35d old) were assigned to 4 treatments; Control(C), $C^+$ Natufermen 0.1%, $C^+$ Natufermen 0.2% $C^+$natufermen 0.3% for 6 week feeding trial. Each treatment had 4 replications of 3 pigs each. Natufermen$^{(R)}$ supplemented treatments tended to increase ADG and ADFI, but there were no significant differences among treatments. Supplementation of Natufermen$^{(R)}$ at the level of 0.2% improved ADFI/ADG(P<0.05). Ammonia gas production from manure was not significantly affected by treatments. All Natufermen$^{(R)}$ treatments increased in DM, crude ash and phosphorus digestibility(P<0.05). Serum IgG decreased as supplemention level of Natufermen$^{(R)}$ increased(P<0.05). Supplemtation of Natufermen$^{(R)}$ at 0.1% and 0.2% increased fecal Lactobacilli population at 3 wks after feeding treated diets(P<0.05). It is concluded that supplementation of Aspergillers oryzae product Natufermen$^{(R)}$ improved feed conversion ratio and digestibility of DM, crud ash and phosphours. Fecal ammonia gas production was not significantly affected but serum IgG level was decreased and CFU of fecal Lactobacilli was increased at 3 wks after feeding Natufermen$^{(R)}$ supplemented diets.

• Journal of Animal Science and Technology
• /
• v.62 no.6
• /
• pp.824-839
• /
• 2020

The current study was aimed to examine the effect of partial or complete replacement of fishmeal (FM) with fermented soybean meal (FSBM) on growth performance, fecal composition, and meat quality in broiler chickens. A total number of 240 one-day-old broiler chicks were randomly allotted into four dietary treatments with six replications and ten birds per one pen. Dietary treatments were followed as; 1) Diet incorporated with 4% FM without FSBM (Control), 2) Diet incorporated with 3% FM and 2% FSBM (FSBM2), 3) Diet incorporated with 2% FM and 3% FSBM (FSBM3) and 4) Diet incorporated with 4% FSBM without FM (FSBM4). Body weight and feed intake were recorded weekly for 35 days of the experimental period. Moreover, fecal samples were collected to evaluate moisture, ash, nitrogen, calcium and phosphorus content on day 21 post-hatch. On day 35, two birds were sacrificed from each pen to measure meat quality parameters and visceral organ weights. Results revealed that, no dietary treatment effect (p > 0.05) was observed either in both body weight or average daily gain of broilers within the entire experimental period while broilers fed FSBM2 increased (p < 0.05) average daily feed intake by 10.07% whereas FSBM4 improved (p < 0.05) feed efficiency ratio by 8.45% compared to birds fed other dietary treatments on day 7 post-hatch. Besides, birds fed FSBM3 obtained the improved (p < 0.05) feed conversion ratio over the birds fed control diet by 7.51% from hatch to day 35 post-hatch (1.60 vs. 1.73). Nevertheless, no difference (p > 0.05) was detected on visceral organ weight, proximate composition and physicochemical characteristics of meat while broilers offered FSBM4 obtained the lowest (p < 0.05) calcium and phosphorous in faces (2.27% and 1.21% respectively) over those offered control feed and other FSBM treatments. In conclusion, FSBM would be a better replacement for ousting FM partially or completely in broiler diet as it did not impair the growth performance and meat quality while reducing the calcium and phosphorous excretion in broilers for 35 days post-hatch.

• Asian-Australasian Journal of Animal Sciences
• /
• v.8 no.4
• /
• pp.393-402
• /
• 1995

Experiments were conducted to evaluate the nutritive values of supplemental L-lysine, liquid and powder type, and DL-methionine in weanling pigs. For feeding trial, 165 weanling pigs were treated in 2 controls; 18 and 16% CP, 6 supplementations of lysine alone to 16% CP diets; 0.1, 0.2 and 0.4% of liquid and powder type each, and 3 supplementations of lysine + methionine to 15% CP diets; 0.05 + 0.025, 0.1 + 0.05 and 0.2 + 0.1%. Pigs were fed for 5 week to investigate the protein sparing effect of supplemental amino acid, and the optimal supplemental level. A metabolic trial included the measurements of digestibilities of dry matter, crude protein, crude fat, crude fiber, energy, phosphorus and amino acids. The liver acinar cell culture was conducted for the protein synthesis activity of the pigs fed each experimental diet. Supplementation of both type of L-lysine in 16% CP diet showed improved daily weight gain and feed efficiency which were compatible with those of pigs fed 18% CP diet. Groups fed liquid lysine did not differ from those fed powder type in growth performance. Supplementation of lysine and methionine to 15% CP diet did not improve growth performance of pigs to the extent that 18% CP diet was fed. In nutrient digestibility, 16% CP control diet showed significantly (p < 0.05) lower crude protein digestibility than any other treatments. Digestibilities of 16% CP diets with lysine supplementation were equal to that of 18% CP control, while digestibilities of 15% CP diets with the supplementation of lysine + methionine was inferior to that of 18% CP control. Supplementation of lysine alone reduced the nitrogen excretion compared to the none supplemented control groups. However, addition of lysine + methionine excreted more nitrogen than controls. Pigs fed diet supplemented with lysine alone, or lysine + methionine excreted less fecal phosphorus than those fed none supplemetation. Retained protein from liver tissue of pigs fed 18% diet was significantly (p < 0.05) greater than those fed 16% CP diet. A significant difference (p < 0.05) was observed in physical type of lysine. Feeding of powder type showed less secreted protein and greater retained protein in the culture of liver acinar cell. It is concluded that supplementation of lysine at the level of 0.1 to 0.2% can spare 2% of dietary protein and reduce nitrogen excretion by 19.3%. Also, no difference in nutritional values was observed between liquid and powder lysine in weanling pigs.

• Journal of Aquaculture
• /
• v.10 no.3
• /
• pp.327-334
• /
• 1997

This study was conducted with Israeli strain of common carp to determine apparent protein, lipid and gross energy digestibility coefficients and apparent calcium and phosphorus availabilities of the following feedstuffs : steam-dried white fish meal (WFM-S), flame-dried white fish meal (WFM-F), brown fish meal (BFM), soybean meal (SBM), corn gluten meal (CGM), sesame meal (SSM), wheat flour (WFL), brewer's yeast (BY) and monocalcium phosphate (MCP). Test diets consisting of a 70:30 mixture of refoernce diet to test ingredient were utilized with chromic oxide as an external indicator. Fecal collection was made during 10 days before the end of feeding trial lasted for 27 days. The best weight gain and feed utilization were found in fish fed MCP containing test diet. Protein digestibility was high for most ingredients, ranging from 81.2% for BY to 91.9% for SBM, with the exception of SSM which was 77.6%. Lipid digestibility coefficients ranged 74% for CGM to 85.8% for two kinds of white fish meal. Digestible energy coefficients were generally high for fish meals, ranging from 77.4% for WFM-S to 81.3% for WFM-F, whereas those for plant feedstuffs were significantly (P<0.05) lower, from 64.5% for CGM to 70.6% for BY. Phosphorus availability from fish meals was variable with a low of 6.6% for WFM-F and a high of 13.6% for WFM-S. Phosphorus availability from SBM and BY was 7.3% and 57.2%, respectively. Calcium and phosphorus availabilities from MCP were 84.6% and 80.6%, respectively. These results provide more precise information concerning nutrients and energy utilization of Israeli strain of common carp and will allow ingredient substitutions in practical diet formulations based on levels of availabile protein and phosphorus.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.12
• /
• pp.1718-1728
• /
• 2011

Two experiments were conducted to investigate the in vitro ability of keratinase to hydrolyze soybean glycinin and ${\beta}$-conglycinin and to evaluate the in vivo effects of keratinase when included in corn-soybean diets with different levels of crude protein and fed to nursery pigs. In experiment 1, a saturated keratinase solution (1 ml) was added to two blank controls of either glycinin or ${\beta}$-conglycinin resulting in the hydrolysis of 94.74% glycinin and 88.89% ${\beta}$-conglycinin. In experiment 2, 190 pigs (8.3${\pm}$0.63 kg BW) were allotted to one of four treatments in a 2${\times}$2 factorial arrangement on the basis of body weight, and sex was balanced among the pens. The effects of crude protein (19 vs. 22%) and keratinase (0 vs. 0.05%) were studied. Each treatment was applied to six pens with seven (two pens) or eight pigs per pen. Pigs were fed the experimental diets for 21 d. Weight gain and feed conversion ratio were improved (p<0.05) with keratinase supplementation while feed intake was reduced (p<0.05). Keratinase supplementation increased (p<0.05) the apparent total tract digestibility of dry matter, energy, crude protein and phosphorus. Keratinase supplementation also increased n-butyric acid in the cecum and colon, lactobacilli and total anaerobe counts in the colon as well as the ratio of villus height to crypt depth in the ileum. Additionally, fecal score, ammonia nitrogen and branch chain volatile fatty acids in the colon, E. coli and total aerobe counts in the colon, crypt depth in the jejunum and ileum as well as serum interleukin-1 and interleukin-6 concentrations were also decreased (p<0.05) by keratinase supplementation. A reduction in dietary crude protein decreased (p<0.05) colon ammonia nitrogen concentration and cecal propionic acid and branch chain volatile fatty acid concentrations. In addition, cecal E. coli counts, colon total anaerobe counts, ileal crypt depth, and serum interleukin-1 and interleukin-6 concentrations were also decreased (p<0.05) with the reduction of dietary crude protein. With the exception of fecal scores, there were no significant interactions between crude protein and keratinase. This study provides evidence that dietary keratinase supplementation improved nursery pig performance by improving intestinal morphology and ecology, thus improving nutrient digestibility and alleviating the inflammatory response.

• Korean Journal of Environmental Agriculture
• /
• v.31 no.3
• /
• pp.235-247
• /
• 2012

BACKGROUND: Gohyeon Stream is the municipal eco-stream of 7.1km in total length which flows through the downtown area of Gohyeon in Geoje city, rising from the watershed of Mundong Water Fall. Gohyeon district in Geoje city has been a rapid growing area centering in Geoje city and then experienced an rapid increase in population. Large amounts of sewage pollutants have been spewed into Gohyeon Stream from its tributaries, due to the lack of sewer system. Gohyeon Stream is laced with unhealthy levels of fecal coliform (FC). Restoration of water quality in Gohyeon Stream is no less inevitable in behalf of its ecosystem and the citizen. In this study, the water quality of Gohyeon Stream and its tributaries was examined temporally and spatially, and their relationships were comparatively analyzed to give useful basic data applying to a restoration project of the water quality of Gohyeon Stream. METHODS AND RESULTS: The samples ware taken at 20 points in Gohyeon Stream and 19 points in its tributaries during the rainy and dry seasons, respectively, and examined on the parameters of pH, temperature, salinity, dissolved oxygen (DO), suspended solid (SS), biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved inorganic nitrogen (DIN; $NH_3$-N, $NO_3$-N, $NO_2$-N), disolved inorganic phosphorus (DIP; $PO_4$-P) and FC. The data were analyzed using a comparative analysis and Pearson's correlation analysis among the parameters. During the rainy season, the concentration of SS was high in the upper region of Gohyeon Stream, and the concentrations of COD, DIN and DIP were low in the upper region and high in the middle and lower regions. During the dry season, the concentration of SS was low and the concentrations of COD, DIN, DIP and FC were high in all regions. The Pearson's correlation analyses showed that the relationships between DO and FC, COD and DIP, and DIN and FC during the rainy season as well as between DO and DIN, SS and FC, COD and DIP, and DIN and DIP during the dry season were significant. CONCLUSION: During the rainy season, the upper region of Gohyeon Stream flowed the high level of SS, the middle region the high level of nutrients due to an agricultural run-off, and the lower region the high level of nutrients due to a sewage inflow. During the dry season, the water quality of Gohyeon Stream was directly and sensitively influenced on the inflow of sewage from the tributaries.

• Journal of Wetlands Research
• /
• v.25 no.4
• /
• pp.326-334
• /
• 2023

The purpose of this study is to use long-term water quality monitoring data from tributaries of the Nakdong River system to identify problematic substances in tributaries by examining the rate of exceedance and increase in water quality targets. In the Nakdong River system, monitoring is conducted once a month for 38 tributaries that require intensive management, and this data was used to analyze trends in exceeding and increasing target water quality at each point. The analysis items are eight items that can be evaluated based on river water quality standards: DO, BOD, COD, TOC, SS, total phosphorus, fecal coliform, and total coliform. As a result of the analysis, the target water quality exceedance rate was more than 50%, and the items with an increasing trend were TOC, fecal coliform and total E. coli counts, and the items with an exceedance rate of less than 50% but an increasing trend were SS. TOC is believed to be caused by an increase in non-degradable substances, and the continued increase in Total Coliform will require management of Total ColiformTotal Coliform in effluent water from sewage treatment facilities in the future.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.7
• /
• pp.985-993
• /
• 2017

Objective: The objective of this study was to evaluate increasing doses of a novel microbial phytase (Cibenza Phytaverse, Novus International, St. Charles, MO, USA) on standardized total tract digestibility (STTD) of P in canola meal (CM), corn, corn-derived distiller's dried grains with solubles (DDGS), rice bran (RB), sorghum, soybean meal (SBM), sunflower meal (SFM), and wheat. Methods: Two cohorts of 36 pigs each (initial body weight = $78.5{\pm}3.7kg$) were randomly assigned to 2 rooms, each housing 36 pigs, and then allotted to 6 diets with 6 replicates per diet in a randomized complete block design. Test ingredient was the only dietary source of P and diets contained 6 concentrations of phytase (0, 125, 250, 500, 1,000, or 2,000 phytase units [FTU]/kg) with 0.4% of $TiO_2$ as a digestibility marker. Feeding schedule for each ingredient was 5 d acclimation, 5 d fecal collection, and 4 d washout. The STTD of P increased (linear or exponential $p{\leq}0.001$) with the inclusion of phytase for all ingredients. Results: Basal STTD of P was 37.6% for CM, 37.6% for corn, 68.6% for DDGS, 10.3% for RB, 41.2% for sorghum, 36.7% for SBM, 26.2% for SFM, and 55.1% for wheat. The efficiency of this novel phytase to hydrolyze phytate is best described with a broken-line model for corn, an exponential model for CM, RB, SBM, SFM, and wheat, and a linear model for DDGS and sorghum. Based on best-fit model the phytase dose (FTU/kg) needed for highest STTD of P (%), respectively, was 735 for 64.3% in CM, 550 for 69.4% in corn, 160 for 55.5% in SBM, 1,219 for 57.8% in SFM, and 881 for 64.0% in wheat, whereas a maximum response was not obtained for sorghum, DDGS and RB within the evaluated phytase range of 0 to 2,000 FTU/kg. These differences in the phytase concentration needed to maximize the STTD of P clearly indicate that the enzyme does not have the same hydrolysis efficiency among the evaluated ingredients. Conclusion: Variations in enzyme efficacy to release P from phytate in various feedstuffs need to be taken into consideration when determining the matrix value for phytase in a mixed diet, which likely depends on the type and inclusion concentration of ingredients used in mixed diets for pigs. The use of a fixed P matrix value across different diet types for a given phytase concentration is discouraged as it may result in inaccurate diet formulation.