• Journal of Nutrition and Health
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• v.28 no.9
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• pp.825-833
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• 1995

This study was performed to investigate the influence of dietary fiber, which from whole food on the serum lipid level and bowel function in rats. The fiber sources of experimental diets were prepared by drying and milling of small water dropwort(WD), mugwort(MW), butterbur(BB) and apple(AP). Each fiber sources was mixed at the 15% level of the diet. The level of dietary fiber in WD, MW, BB diets was similar but that in AP diet was slightly low. Male rats of Sprague-Dawley strain were blocked into 5 groups ; WD, MW, BB, AP, FF(fiber free). The animals were fed ad libitum each of experimental diets for 37 days. Food intake of AP and FF groups were than other groups, FF group was lower than other grous in food efficiency ratio. MW group showed highest level in fecal weight and fecal water content and group has the longest transit time compare to other groups. Serum triglyceride level was not significantly different among groups. BB group showed the lowest level in the serum total cholesterol. WD and MW groups showed slightly higher level in the serum HDL cholesterol than other groups. Mucosa weight was not significantly different among groups. The activity of maltase in mucosa of small intestine was highest in FF groups. Absorption rates of calcium were not significantly different among groups. Absorption rate of magnesium was higher in FF group compare to others. And, absorption rates of phosphorus in MW and FF groups were slightly lower than other groups.

• Journal of Nutrition and Health
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• v.22 no.6
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• pp.516-530
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• 1989

A 44-day metabolic study was conducted to compare the effects of soy protein and meat protein on N, Ca, P, and Mg metabolism in young Korean males. Meat protein diet(MPD) and soy protin diet(SPD) were provided for 3 weeks each. For MPD, meat protein Supplied 50.3% of total protein and mean daily suplies of Ca, P, and Mg were 33.28mg, 1013.01mg, 226.81mg respectively. For SPD, soy protein supplied 51.8% of total protein and mean contents of Ca, P and Mg were 737.86mg, 1261.76mg, and 451.3mg. Mean daily fecal and urinary excretions of Ca were 350.6mg and 113.3mg for MPD and 515.0mg and 118.3mg for SPD. Apparent absorption rates in MPD and SPD were 4.2mg in MPD and 700.4mg and 475.1mg SPD. Apparent absorption rates were 65.6% in MPD and 44.5% in SPD(p<0.01). For Mg, fecal and urinary excretions were 144.8mg and 81.2mg in MPD and 292.4mg and 65.5mg in SPD. Apparent absorption rates were 37.5% in MPD and 35.2% in SPD. The correlations between urinary Ca, P, and their balance were significant (p<0.001) in MPD, but not in SPD. Urinary Ca was significantly correlated with urinary N, P, Mg(p<0.001) in MPD, but not in SPD. From these results, meat protein seems to increase the excretions of major minerals compared to soy protein.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.2
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• pp.248-255
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• 2012

Three levels of boron (0, 30, 60 ppm) were supplemented in practical corn-soybean based starter and grower diets, containing either adequate or inadequate Ca or P. A total of 1,800, 1-day-old sexed broiler chicks were assigned to six dietary treatments and fed with the experimental diets for 42 days. Boron improved the overall feed conversion ratio, but increased body weight only at 21 days of age (p<0.01). Boron decreased feed intake in the case of feeding on a diet deficient in Ca and P, and tended to increase feed intake when birds received a diet adequate in Ca and P, signifying significant boron by Ca-P interaction (p<0.01). Mortality was not influenced by boron (p>0.05). Dietary Ca and P deprivation reduced body weight and feed consumption significantly, but did not influence the feed conversion ratio and mortality (p>0.05). Serum Ca level, ALP and ALT activities were not influenced either by dietary Ca and P deficiency or boron supplementation. Serum P content increased with respect to boron at 30 ppm. Bone breakage strength was not affected by dietary variables. Tibia ash, Ca and P were increased in response to the supplementation diet with 30 ppm boron, whereas 60 ppm showed no effect in most cases. Accordingly, the dietary boron supplementation of 30 ppm significantly decreased fecal Ca and P excretion, while there was a numerical decline in the 60 ppm boron as compared to the 0 ppm boron group. Data presented herein indicated that boron, either at the 30 ppm or 60 ppm supplementation level, was effective in conversion of feed to body weight, whereas only boron at 30 ppm contributed to the mineralization of bone thereby augmenting more Ca and P while excreting less through faeces.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.8
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• pp.1191-1197
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• 2002

This experiment was conducted to study the effects of replacing corn with Chinese brown rice on performance and apparent fecal digestibility of nutrients in weanling pigs. Seventy-two cross-bred (Duroc${\times}$Landrace${\times}$Large White) weanling pigs, weighing an average of $7.38{\pm}0.06kg$, were randomly assigned to either a corn-soybean meal diet (treatment 1), a corn-brown ricesoybean meal diet (treatment 2) and a brown rice-soybean meal diet (treatment 3). The brown rice used in this experiment was husked from one kind of early, long grain, non-glutinous rice (ELGNR, indica rice). The growth performance of weanling pigs in the experiment was similar during weeks 1 and 2, but feed conversion ratio (FCR) was significantly better in treatment 2 during weeks 3 and 4. During week 2, only the digestibility of organic matter in treatments 2 and 3 was significantly higher than that in treatment 1. During week 4, the digestibility of gross energy, ether extract, organic matter and dry matter in brown rice treatment, especially in treatment 3, were significantly higher than in treatment 1. At the end of the experiment, the concentration of serum urea nitrogen of pigs in treatment 2 was lower than that in treatment 1 and 3 and no difference was found in serum glucose and phosphorus. It could be concluded from this experiment that replacing 50% of corn with brown rice in the corn-soybean meal diet caused no negative effects to weanling pig performance, and apparent fecal digestibility was unaffected. It is feasible to replace corn with brown rice in weanling pig diets in southern China.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1666-1672
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• 2003

The objective of this study was to evaluate the potential of an alumninosilicate clay, marketed under the trade name Biotite V, to improve growing-finishing pig performance and to determine its effects on nutrient digestibility and excretion. Sixty crossbred pigs (22.3${\pm}2.7kg$, Camborough 15 Line female${\times}$Canabred sire) were assigned on the basis of sex, weight and litter to one of four dietary treatments in a $2{\times}4$ (two sexes and four treatments) factorial design experiment. The experimental diets were based on barley and soybean meal and contained 0, 0.25, 0.5 or 0.75% biotite during the growing period (22.3-60.5 kg) and 0, 0.5, 1.0 or 1.5% biotite during the finishing period (60.5-110.3 kg). Each pig was allowed access to its own individual feeder for 30 min twice daily (07:00 and 15:00 h). Individual pig body weight, feed consumption and feed conversion were recorded weekly. The pigs were slaughtered at a commercial abattoir when they reached an average weight of 110.3 kg. Carcass weight was recorded and dressing percentage calculated. Carcass fat and lean measurements were obtained with a Destron PG 100 probe between the 3rd and 4th last ribs, 70 mm of the midline. Total tract digestibility coefficients for dry matter, energy, nitrogen and phosphorus were determined using three males and three females per treatment starting at an average weight of $52.2{\pm}3.8kg$. These pigs were housed under identical conditions as those used in the growing stage and were fed the same diets modified only by the addition of 0.5% chromic oxide as a digestibility marker. Over the entire experimental period (22.3-110.3 kg), daily gain was unaffected (p>0.05) by the inclusion of biotite in the diet. There was a cubic response for feed intake (p=0.06) and a quadratic response (p=0.07) for feed conversion due to biotite. Feeding biotite produced no significant (p>0.05) linear or quadratic effects on any of the carcass traits measured. Dry matter digestibility decreased linearly (p=0.02) with increasing levels of biotite in the diet. However, digestibility coefficients for energy, nitrogen and phosphorus were unaffected (p<0.05) by biotite inclusion. Lactobacilli and enterobacteria numbers were unaffected by inclusion of biotite while Salmonella was not detected in any of the fecal samples. The overall results of this experiment indicate that biotite inclusion did not reduce fecal excretion of nitrogen or phosphorus and failed to improve nutrient digestibility. Neither growth rate nor carcass quality was improved while a modest improvement in feed conversion was observed at lower levels of inclusion. Based on the results of this experiment, it would be difficult to justify the routine inclusion of biotite in diets fed to grower-finisher pigs. Whether or not a greater response would have been obtained with pigs of a lower health status is unknown.

• Journal of Nutrition and Health
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• v.28 no.11
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• pp.1049-1055
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• 1995

This study was designed to investigate the effects of calcium supplementation on calcium metabolism in seven healthy college women, aged from 19 to 21 years old. For this purpose, metabolic studies were conducted for two weeks. During the first week, the subjects ate experimental diet which nutrients composition was similar to their usual intake. And during the consecutive second week, they ate the same experimental diet supplemented with 500mg of calcium daily. The results were summarized as follows ; 1) Fecal excretion of calcium increased significantly (P<0.05), but urinary excretion of that did not show any change after supplementary intake of calcium. 2) Mean apparent calcium absorption was 28.5% and retention was 182mg/day when subjects ate the experimental diet without calcium supplementation. Calcium retention was significantly ate the experimental diet without calcium supplementation. Calcium retention was decreased to 24.1% by additional intake of calcium. 3) Phosphorus balance did not show any change after additional intake of calcium. 4) Serum calcium level was also not changed by additional intake of calcium. 5) Serum calcium level increased significantly(P<0.05) but serum phosphorus level did not show any change after additional intake of calcium. The above results showed that supplementation of 500mg calcium daily can be helpful to increase calcium retention as well as the peak bone mass in young women eating usual Korean diets.

• Journal of the Korean Society of Food Science and Nutrition
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• v.18 no.2
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• pp.229-237
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• 1989

The effects of ethanol and dietary protein levels on the calcium and phosphorus metabolism were investigated in 15-week-old male rats. Rats were divided into 4 groups ; control group(16% protein, 16 PC) and 8%(8 PE), 16%(16 PE), and 24% protein groups (24 PE) to which was given 5% ethanol mixed with their drinking water. Body weigh gain, organ weight, hemoglobin content, and hematocrit value were not affected by either ethanol or dietary protein levels. Calcium concentrations in spleen were significantly decreased in the ethanol groups than those in control group. Calcium and Phosphorus levels in femur, serum, liver, kidney, and muscle were normal. Among ethanol treated groups, fecal excretions of calcium were a little more decreased, but urinary excretions, balances and apparent absorption rate of calcium were a little more increased in higher percentage of protein group than lower percentage. Urinary phosphorus excretions in the ethanol treated groups were significantly decreased compared with the control group. Among ethanol treated groups, phosphorus balance and apparent phosphorus absorption rate of 24 PE group were significantly higher than those of 8 PE and 16 PE groups.

• Journal of Aquaculture
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• v.7 no.3
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• pp.165-175
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• 1994

Attempts were made to find out nitrogen and phosphorus loads to aquatic environment resulting from feeding Nile tilapia (Oreochromis niloticus). Two different size groups, small and large, were used. The average sizes of small and large tilapia were 65.2 g and 389.2 g respectively, and three kinds of commercial diets were used for each size. The 3 kinds of commercial diets for tilapia contained in average 33.8% crude protein ($5.4\%$ nitrogen) and $1.4\%$ total phosphorus. The load of nitrogen and phosphorus were measured by subtracting the amounts of nutrients retained in the body of fish from consumed nutrients. Sixty, five percentage of total feces was excreted within 24 hours after feeding at $23^{\circ}C$. Nitrogen content in the feces was higher in large fish than small ones. The apparent digestibility of dietary protein for small and large tilapia was $90.0\%$ and $89.7\%$, respectively. Availability of dietary phosphorus for small and large tilapia was $44.7\%\;and\;51.4\%$, respectively. The total load of nitrogen and phosphorus per 1 metric ton of tilapia production was 49.5kg and 6.3kg, respectively, for small ones with feed conversion ratio (FCR) of 1.4, and 61.3 kg and 13.4kg, respectively, for large ones with the FCR of 1.8. Nitorgen balance appeared that small and large tilapia excreted $7.1\%\;and\;9.9\%$ of consumed nitrogen through fecal-nitrogen and $55.5\%\;and\;62.3\%$ through urine and gills, retaining $37.4\%\;and\;27.8\%$ in the body, respectively. These results show that small fish pollute less than large fish, excreting less and retaining more nutrients in the body.

• Journal of Animal Science and Technology
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• v.52 no.4
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• pp.271-280
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• 2010

This study was conducted to investigate the effects of Aspergillus niger-derived multi-enzyme complex supplementation to feedrestricted lactating sows on performances, milk yield, blood profiles, and manure excretion as compared with ad libitum-fed sows without supplementation of enzyme. Fifty multiparous lactating Berkshire sows were allotted to 5 treatments of 10 sows per treatment during a 28-d lactation period and litter per sow was standardized to 9 suckling piglets. Treatments were ad libitum-fed sows without enzyme and feed-restricted sows supplemented with four increasing levels (0, 0.02, 0.04 and 0.08%) of multi-enzyme complex derived from Aspergillus niger. Blood samples from all sows were collected to determine serum metabolite concentrations before the morning feeding on d 27 of lactation. Litter body weight and a piglet weight at weaning, and litter weight gain significantly (P<0.05) increased with increasing levels of multi-enzyme complex, but there was no significant difference between ad libitum-fed sows without enzyme and feed-restricted sows supplemented with multi-enzyme complex. Body condition score and backfat depth at weaning significantly (P<0.05) increased as multi-enzyme complex level increased. Lactational backfat depth tended (P>0.05) to less decrease with increasing levels of enzyme complex. Serum inorganic phosphorus and non-esterified fatty acid concentrations significantly (P<0.05) increased with increasing levels of enzyme complex. Daily milk yield was not significantly different across treatments, but milk fat yield significantly (P<0.05) increased as multi-enzyme complex level increased. Manure output was significantly (P<0.01) higher for ad libitum-fed sows than for feed-restricted sows, but there was no significant difference among feed-restricted sows supplemented with increasing levels of multi-enzyme complex. Fecal phosphorus amount significantly (P<0.05) decreased with increasing levels of multi-enzyme complex. Feed costs of sows per litter weight gain were reduced by 1.25% to 9.67% with increasing levels of multi-enzyme complex as compared with ad libitum-fed sows without enzyme. The results indicated that multi-enzyme supplementation to feed-restricted lactating sows not only increased litter performances, but also was comparable to ad libitum-fed sows, resulting in reduced feed costs. Moreover, the reduction of fecal phosphorus amount with increasing levels of enzyme complex would contribute to the reduction of environmental pollution.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.107-119
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• 2008

To determine the true digestible phosphorus (TDP) requirement of growing pigs, two experiments were designed with the experimental diets containing five true digestible P levels (0.16%, 0.20%, 0.23%, 0.26% and 0.39%) and the ratio of total calcium to true digestible P (TDP) kept at 2:1. In Experiment 1, five barrows (Duroc${\times}$Landrace${\times}$Yorkshire) with an average initial body weight of 27.9 kg were used in a $5{\times}5$ Latin-square design to evaluate the effect of different dietary P levels on the digestibility and output of P and nitrogen. In Experiment 2, sixty healthy growing pigs (Duroc${\times}$Landrace${\times}$Yorkshire) with an average body weight (BW) of 21.4 kg were assigned randomly to one of the five dietary treatments (12 pigs/diet), and were used to determine the true digestible phosphorus (TDP) requirement of growing pigs on the basis of growth performance and serum biochemical indices. The results indicated that the true digestibility of P increased (p<0.05) linearly with increasing dietary TDP level below 0.26%. The true P digestibility was highest (56.6%) when dietary TDP was 0.34%. Expressed as g/kg dry matter intake (DMI), fecal P output increased (p<0.05) linearly with increasing P input. On the basis of g/kg fecal dry matter (DM), fecal P output was lowest for Diet 4 and highest (p<0.05) for Diet 5. The apparent digestibility of crude protein (CP) did not differ (p>0.05) among the five diets, with the average nitrogen output of 12.14 g/d and nitrogen retention of 66% to 74% (p>0.05), which suggested that there was no interaction between dietary P and CP protein levels. During the 28-d experimental period of Experiment 2, the average daily gain (ADG) of pigs was affected by dietary TDP levels as described by Eq. (1): $y=-809,532x^4+788,079x^3-276,250x^2+42,114x-1,759$; ($R^2=0.99$; p<0.01; y = ADG, g/d; x = dietary TDP, %), F/G for pigs by Eq. (2): $y=3,651.1x^4-3,480.4x^3+1,183.8x^2-172.5x+10.9$ ($R^2=0.99$; p<0.01; y = F/G; x = dietary TDP, %), and Total P concentrations in serum by Eq. (3): $y=-3,311.7x^4+3,342.7x^3-1,224.6x^2+195.6x-8.7$ (R2 = 0.99; p<0.01; y = total serum P concentration and x = dietary TDP, %). The highest ADG (782 g/d), the lowest F/G (1.07) and the highest total serum P concentration (3.1 mmol/L) were obtained when dietary TDP level was 0.34%. Collectively, these results indicate that the optimal TDP requirement of growing pigs is 0.34% of the diet at a total Ca to TDP ratio of 2:1.