• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.6
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• pp.697-703
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• 2018

In 2017, total coliform (TC), fecal coliform (FC) and fecal Streptococci (FS) bacteria were examined in seawater samples collected at coastal sites on Jeju Island (Aewol as a control, Namwon, and Daejeong) and at Pohang (Yeongil as a control, Guryongpo-North, and Guryongpo-South) to examine the correlations between the density of fish farms and distributions of the indicator bacteria. Only a few TC, FC, and FS colonies were detected in all of the samples obtained from Jeju Island. Of note, 2,000 and 1,000 CFU of FS $100mL^{-1}$ were detected in samples from Guryongpo-South in June and August, respectively. Although the total area of approved fish farms located within 5 km of the sampling point at Guryongpo-South is 5-16 times smaller than in other regions, the number of indicator bacteria was highest in this region. Therefore, microbiological pollution in the Guryongpo-South region might be due to sources other than the effluent released from nearby fish farms.

• Journal of Environmental Health Sciences
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• v.36 no.2
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• pp.95-107
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• 2010

Hand-washing is one of the major factors in personal hygiene and public health. This study was undertaken to investigate the hygienic behavior of food-service employees, focusing on awareness of hand washing, hand washing practices, and the load of index microorganisms (aerobic plate count, total and fecal coliforms, Escherichia coli, and Staphylococcus aureus) on the hands of food-service employees. A questionnaire survey completed by direct interview, direct observation of restrooms by the researcher and trained observers, and microbiological examination according to the Food Code of Korea were carried out. In the survey, a positive attitude toward hand washing compliance was reported; however, improper hand washing and poor hand hygiene of the food-service employees were seen under direct observation. Significant differences (p<0.05) were found between the questionnaire survey and the direct observations in hand washing compliance after using the toilet, duration of hand washing, use of hand washing agents, use of hand washing tools, washing of different parts of the hands, hand-drying method, temperature of water, and method of turning off the water. Samples taken from employees' hands before washing showed higher levels of bacteria than those taken during work and/or after washing (p<0.05). Poor hand washing practices were indicated by the positive results for total and fecal coliforms, E. coli, and S. aureus on the hands of some food-service employees. This study showed that there is a marked difference between the food-service employees' awareness of hand-washing and their actual hand-washing practices. The poor hand hygiene of and improper hand washing by the food-service employees should be addressed for improved food safety.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.6
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• pp.561-569
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• 2012

To evaluate the bacteriological water quality in Yongnam-Gwangdo, located in western Jinhae Bay, seawater samples were analyzed using sanitary indicator bacteria at 57 sampling stations. According to survey results from January 2007 to December 2009, the range of the geometric mean and the estimated 90th percentile for coliforms and fecal coliforms in the samples were <1.8-16.5 and 1.8-246.8 MPN/100 mL and <1.8-7.1 and 1.8-74.8 MPN/100 mL, respectively. The samples, including those taken from stations located in Wonmunman, Gwangdo, and Dangdong, showed high levels of microbial contamination caused by the climate and weather patterns in the marine environment. The bacteriological water quality in the area met Korean criteria for a designated shellfish growing area for export and National Shellfish Sanitation Program criteria for an approved shellfish growing area, except at station #49. A total of 24 direct pollution sources were discharged into the shellfish growing area. The radius of impact was calculated for each pollution source to assess the effect on the shellfish growing area. The calculated radius of impact for most of the pollution sources was below 300 m. However, the radius of impact for the combined pollution sources in Kyeonnaeryang was 93-1973 m. There were significant differences between the calculated closed sea area and actual monitoring results. The closed sea area values calculated from the fecal coliform load in drainage water tended to be higher than the actual monitoring results. Tidal currents and environmental factors such as salinity, water temperature, sunlight, and microbiological factors affect the survival of fecal indicator bacteria in seawater.

• Journal of Food Hygiene and Safety
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• v.11 no.3
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• pp.177-187
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• 1996

Time-temperature relationship and microbiological quality were assessed and critical control points were identified through hazard analysis during the phases of production in two different packaged meals (Dosirak) manufacturing establishments (A, B:Kim Pab). Microbiological tests on foods, equipments and utensils were done according to standard procedures and included total plate count, coliforms and fecal coliforms. The results of the study are summarized as follows : time-temperature control management was needed because time-temperature abuse more than 8 hours at dangerous temperature zone (5-6$0^{\circ}C$) was observed from pre-preperation to distribution phase; Poor sanitary practices of employees were observed in hand washing and using disposable gloves; Microbiological analysis results of equirpments and utensils showed possible cross-contamination risks when foods were contacted with them; Kim Pab needed thorough quality control because it included various mixed ingredients of cooked and uncooked and had many apportunities of cross-contamination either by equipments or hands through whole production processes.

• Journal of The Korean Society of Agricultural Engineers
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• v.47 no.4
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• pp.75-85
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• 2005

A study was performed to examine the effects of reclaimed-water irrigation on microorganism con-centration in ponded-water of paddy rice plots. Several treatments were used and each one was triplicated to evaluate the change of indicator microorganisms (total coliforms (TC), fecal coliforms FC), and E. coli) concentrations in 2003 and 2004 growing seasons. Their concentrations increased significantly right after irrigation, but decreased about $45\%$ in 24 hours. It implies that agricultural activities such as plowing and fertilizing should be practiced one or two days after irrigation considering health-risks. Treatments with UV-disinfected water irrigation demonstrated significantly lower concentrations than others including control plots where natural surface water was irrigated. The monitoring results from actual paddy rice fields and experimental paddy plots showed that concentrations of indicator microorganisms ranged from $10^2\;to\;10^5$ MPN/100mL. A comprehensive assessment of existing agricultural practices and a thorough monitoring in the field as well as treatment-plots are recommended to make more realistic national guidelines more applicable. UV-disinfected water irrigation reduced microorganism concentrations in paddy fields down to below the concentration of conventional paddy rice culture, and is thought to be an effective and feasible measure fur agricultural reuse of secondary effluent.

• Journal of the Korean Society of Industry Convergence
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• v.16 no.2
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• pp.41-46
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• 2013

On the purpose of helping the inhabitants living in farming, fishing villages, and islands for more safe and hygienic water from simple waterworks, experimental investigations were performed concerning the development of a water purifier with silver nanomaterial packed, having a function of the auto-disinfection. The results show as follows through such filteration and auto-disinfection processes. It is possible to get hygienic and safe water, for example, more than 95% of general bacteria, total coliforms, and fecal coliforms were removed. It is also possible to get good-quality water, for 49.4% of spent potassium permanganate and 85% and 63% of turbidity and conductivity were removed respectively. It is a very effective equipment, for 100% cost reduction of used chemicals was achieved by no-chemical disinfection process and THM was not generated.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.3
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• pp.1-7
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• 2015

The objective of this study was to compare fecal microbes survival in soil between compost surface application and soil incorporation. The survival experiment was conducted in six styrofoam beds ($510{\times}325{\times}305(mm)$ in size) filled with sandy loam soil. A half of six boxes were received by compost surface application, while the other half were treated with compost-soil mixture. Duplicated surface and surbsurface soil (20 cm depth) samples were collected at various interval up to 50 days and analyzed for the determination of fecal coliforms and E. coli numbers. As expected, surface applied beds demonstrated two to three magnitudes order greater in both the study microorganisms as compared to soil incorporated beds. Microbial inactivation rate of soil surface was twice as great as subsurface soil condition probably due to exposure to sun light and environmental conditions including moisture loss. When rainfall occurred, microbes on the surface were transported into soil along with water movement. It was concluded that surface compost application may be easier to apply but pose higher risk of human exposure to microbes. Winter compost application may be favorable in alleviating health risk by giving some time for inactivation compared to spring application.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.6
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• pp.614-622
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• 2010

The sanitary quality of a shellfish-growing area in Gangjinman, Korea, was assessed through a bacteriological examination of seawater form January 2007 to December 2009. The range of the geometric mean (GM) and the estimated 90th percentile for most-probable-number (MPN) values for fecal coliforms in samples collected in the designated area were 2.0-2.4 and 6.1-11.5 MPN/100 mL, respectively. Therefore, the bacteriological quality of seawater in the designated area in the Gangjinman area met the National Shellfish Sanitation Program (NSSP, USA) guidelines for approved areas. Also, the sanitary status of shellfish harvested from the designated area met the Korean Shellfish Sanitation Program (KSSP) fecal coliform criterion (<230 MPN/100 g). However, the degree of bacteriological contamination increased rapidly, and water quality exceeded the criteria for approved areas when large amounts of rain fell before the sampling date. Fortunately, fecal pollutants did not affect the bacteriological quality of a buffer zone established between the shoreline and the designated area. These results indicate that the current buffer zone established around the designated area protects the designated area properly.

• Fisheries and Aquatic Sciences
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• v.17 no.2
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• pp.197-201
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• 2014

This study was performed to identify and assess food hazards during dry laver processing. Samples including raw materials, intermediates, and finished products during dried-laver processing were collected from seven dried-laver processing facilities, and microbial analyses were conducted. Microbial levels such as total coliforms and total viable cell count (TVC) increased as the processing steps progressed. TVC and total coliforms ranged from <30 to $9.1{\times}10^7$ CFU/g and <18 to 27,600 MPN/100 g for intermediates and finished products obtained during dried-laver processing, respectively. However, no fecal coliform was detected in the samples. Additionally, food-borne bacteria including Bacillus cereus, Listeria monocytogenes, Salmonella sp., Staphylococcus aureus, and Vibrio parahaemolyticus were not identified in finished products. For heavy metal content, arsenic ranged from 30.18 to 39.05 mg/kg, mercury from 0.005 to 0.009 mg/kg, and cadmium from 0.076 to 0.318 mg/kg dry mass in all finished products. However, lead was not detected in samples tested in this study. In conclusion, dried-laver products were safe based on the levels of food-borne bacteria and heavy metal contents. However, it is important to reduce total viable cell counts and total coliforms during dried-laver processing.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8
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• pp.1137-1144
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• 2019

Objective: This experiment was designed to evaluate the effects of Agave tequilana inulin and Lactobacillus casei (L. casei) on growth performace, hematological variables, serum metabolites, and total coliforms in nursing lambs. Methods: The experimental design was completely randomized; treatments were T1, control (pre-starter concentrate, PC), T2: T1+2% inulin, and T3: T1+2% inulin+L. casei; treatments were compared with Tukey test ($p{\leq}0.05$); and 45 new born $Kathadin{\times}Dorset lambs$ ($4.8{\pm}0.8kg$ birth weight) were the experimental units (15 per treatment). The variables were daily weight gain (DWG), dry matter intake and diarrheas incidence (%) during 56 d. Twenty-four hours after birth and at the end of the experiment, blood samples were collected to evaluate hematological variables and serum metabolites. Besides, the populations of total coliforms and lactobacilli were estimated in fecal samples. Results: Addition of agave inulin and L. casei increased ($p{\leq}0.05$) DWG 356, 384, and 415 g/d, weaning weight 24.92, 26.18, and 28.07 kg, as well as lactobacilli population 5.79, 6.32, and $6.48Log_{10}cfu/g$, for T1, T2, and T3, respectively. Lambs fed L. casei had decreased ($p{\leq}0.05$) populations of total coliforms (T1 = 6.18, T2 = 5.77, and $T3=5.07Log_{10}cfu/g$), diarrheas incidence (T1 = 11.67%, T2 = 8.33%, and T3 = 5.0%), and serum cholesterol concentration (11% in T2 and 13% in T3, compared to control). Conclusion: The combination of Agave tequilana inulin and L. casei increases weight gain and improves intestinal health by reducing coliforms and diarrheas incidence in $Katahdin{\times}Dorset$ lambs during the pre-weaning period.