• Molecules and Cells
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• 제46권7호
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• pp.441-450
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• 2023

β-Catenin (Ctnnb1) has been shown to play critical roles in the development and maintenance of epithelial cells, including the retinal pigment epithelium (RPE). Ctnnb1 is not only a component of intercellular junctions in the epithelium, it also functions as a transcriptional regulator in the Wnt signaling pathway. To identify which of its functional modalities is critically involved in mouse RPE development and maintenance, we varied Ctnnb1 gene content and activity in mouse RPE lineage cells and tested their impacts on mouse eye development. We found that a Ctnnb1 double mutant (Ctnnb1^dm), which exhibits impaired transcriptional activity, could not replace Ctnnb1 in the RPE, whereas Ctnnb1^Y654E, which has reduced affinity for the junctions, could do so. Expression of the constitutively active Ctnnb1^∆ex3 mutant also suppressed the development of RPE, instead facilitating a ciliary cell fate. However, the post-mitotic or mature RPE was insensitive to the loss, inactivation, or constitutive activation of Ctnnb1. Collectively, our results suggest that Ctnnb1 should be maintained within an optimal range to specify RPE through transcriptional regulation of Wnt target genes in the optic neuroepithelium.

• 한국환경농학회지
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• 제42권3호
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• pp.193-202
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• 2023

Residue monitoring of propiconazole (PCZ) in cabbage, shallot, and spinach was conducted under multi-trial greenhouse conditions. This study aimed to understand the fate of the applied fungicide in these vegetables. Furthermore, the associated health risk of PCZ in leafy vegetables was assessed through dietary risk assessment. Commercially available PCZ (22% suspension concentrate) was administered thrice according to the OECD fungicide application interval guideline. The plant samples were extracted using a slightly modified QuEChERS technique and analyzed using gas chromatography-tandem mass spectrometry. The average PCZ recovery was between 84.5% and 117.6%, with a <5% coefficient of variance. The dissipation of PCZ residue in cabbage, shallot, and spinach after 14 days was 96%, 90%, and 99%, respectively, with half-lives of <5 days. Meanwhile, dietary risk assessments of PCZ residues in the studied vegetables using the risk quotient (RQ) were significant < 100 (RQ < 100). Thus, the population groups considered in this study were not at substantial risk from consuming leafy vegetables sprayed with PCZ following critical, good agricultural practices.

• 한국군사과학기술학회지
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• 제26권1호
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• pp.102-112
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• 2023

This research mainly focuses on the transport and dispersion of chemical agent plume according to the Lagrangian Puff Model and Lagrangian Particle Model of NBC_RAMS(Nuclear, Biological, Chemical Reporting And Modeling S/W System). NBC_RAMS was developed with the purposes of estimating the fate of Chemical, Biological, and Radioactive(CBR) agent plumes and evaluating damages in the Republic of Korea. First, it calculates the local weather pattern, i.e. wind speed, wind direction, and temperature, by considering the effects of land uses and topography. The plume behaviors are calculated by adopting the Lagrangian Puff Model(LPFM) or Lagrangian Particle Model(LPTM). In this research, we assumed a virtual chemical agent exposure event in a stable atmospheric condition during the summer season. The plume behaviors were estimated by both LPFM and LPTM on the used area(urbanized and dry area) and the agricultural land. The higher heat flux in the used area led to stronger winds and further downward movement moving of the chemical agent than the farmland. The lateral dispersion of the chemical plume was emphasized in the Lagrangian Puff Model because it adopted Gaussian distribution.

• Nuclear Engineering and Technology
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• 제55권4호
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• pp.1476-1484
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• 2023

Laboratory experiments and point monitoring of reservoir sediments have proven that stable sulfate reduction (SSR) can lower the concentrations of toxic metals and sulfate in acidic groundwater for a long time. Here, we hypothesize that SSR occurred during in situ leaching after uranium mining, which can impact the fate of acid groundwater in an entire region. To test this, we applied a sulfur isotope fractionation method to analyze the mechanism for natural attenuation of contaminated groundwater produced by acid in situ leaching of uranium (Xinjiang, China). The results showed that δ³⁴S increased over time after the cessation of uranium mining, and natural attenuation caused considerable, area-scale immobilization of sulfur corresponding to retention levels of 5.3%-48.3% while simultaneously decreasing the concentration of uranium. Isotopic evidence for SSR in the area, together with evidence for changes of pollutant concentrations, suggest that area-scale SSR is most likely also important at other acid mining sites for uranium, where retention of acid groundwater may be strengthened through natural attenuation. To recapitulate, the sulfur isotope fractionation method constitutes a relatively accurate tool for quantification of spatiotemporal trends for groundwater during migration and transformation resulting from acid in situ leaching of uranium in northern China.

• 분석과 대안
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• 제3권1호
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• pp.29-61
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• 2019

Within the discipline of International Relations (IR), the literatures on global governance (GG) and great power management (GPM) at best ignore each other, and at worst treat the other as a rival or enemy. On the one hand, the GPM literature, like both realism in all its forms, and neoliberalism, takes for granted the ongoing, disproportionate influence of the great powers in the management of the international system/society, and does not look much beyond that. On the other hand, the GG literature emphasizes the roles of smaller states, non-state actors and intergovernmental organizations (IGOs), and tends to see great powers more as part of the problem than as part of the solution. This paper argues that the rise to prominence of a non-traditional security agenda, and particularly of human security, has triggered a de facto merger of GPM and GG that the IR literature usually treated as separate and often opposed theories. We use the Ebola crisis of 2014-15 to show how an issue framed as human security brought about a multi-actor response that combined the key elements of GPM and GG. The security framing overrode many of the usual inhibitions between great powers and non-state actors in humanitarian crises, including even the involvement of great power military forces. Through examining broadly the way in which the Ebola crisis is tackled, we argue that in an age of growing human security challenges, GPM and GG are necessarily and fruitfully merging. The role of great powers in this new human security environment is moving away from the simple means and ends of traditional GPM. Now, great powers require the ability to cooperate and coordinate with multiple-level actors to make the GG/GPM nexus more effective and sustainable. In doing so they can both provide crucial resources quickly, and earn respect and status as responsible great powers. IGOs provide legitimation and coordination to the GPM/GG package, and non-state actors (NSAs) provide information, specialist knowledge and personnel, and links into public engagement. In this way, the unique features of the Ebola crisis provide a model for how the merger of GPM and GG might be taken forward on other shared-fate threats facing global international society.

• The Korean Journal of Physiology and Pharmacology
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• 제27권4호
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• pp.407-416
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• 2023

The regeneration of myocardium following acute circulatory events remains a challenge, despite numerous efforts. Mesenchymal stem cells (MSCs) present a promising cell therapy option, but their differentiation into cardiomyocytes is a time-consuming process. Although it has been demonstrated that PSME4 degrades acetyl-YAP1, the role of PSME4 in the cardiac commitment of MSCs has not been fully elucidated. Here we reported the novel role of PSME4 in MSCs cardiac commitment. It was found that overnight treatment with apicidin in primary-cultured mouse MSCs led to rapid cardiac commitment, while MSCs from PSME4 knock-out mice did not undergo this process. Cardiac commitment was also observed using lentivirus-mediated PSME4 knockdown in immortalized human MSCs. Immunofluorescence and Western blot experiments revealed that YAP1 persisted in the nucleus of PSME4 knockdown cells even after apicidin treatment. To investigate the importance of YAP1 removal, MSCs were treated with shYAP1 and apicidin simultaneously. This combined treatment resulted in rapid YAP1 elimination and accelerated cardiac commitment. However, overexpression of acetylation-resistant YAP1 in apicidin-treated MSCs impeded cardiac commitment. In addition to apicidin, the universal effect of histone deacetylase (HDAC) inhibition on cardiac commitment was confirmed using tubastatin A and HDAC6 siRNA. Collectively, this study demonstrates that PSME4 is crucial for promoting the cardiac commitment of MSCs. HDAC inhibition acetylates YAP1 and facilitates its translocation to the nucleus, where it is removed by PSME4, promoting cardiac commitment. The failure of YAP1 to translocate or be eliminated from the nucleus results in the MSCs' inability to undergo cardiac commitment.

• 영어영문학
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• 제56권4호
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• pp.691-717
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• 2010

Chaucer's Troilus and Criseyde is a tale of love framed by an overarching pattern of death, set in the war-torn and doomed Troy, from which the lovers cannot separate their fate. Compared with Boccaccio's poem, the attention paid to death in Chaucer's version underlies his complex treatment of love. Above all, the language of death in Chaucer's poem provides the thread from which the entangled web of love is woven. Death together with desire pervades the language and rhetoric of the poem, prominent not only in the courtly love tropes, but also in the characters' asides and speeches. The prominence of these two concepts, desire and death, seem to be central to the various issues that the poem contains explicitly and implicitly. That is, two concepts are the basis for the breadth and depth of Chaucer's examination of love in light of the social and political realities of late fourteenth century England. The language of death in Chaucer's poem reflects the powerful influence on his imagination. With the devastation wrought by the plague and the changing fortunes of England in the war with France, Chaucer's world was once saturated in death, and one that could amply parallel the turn from prosperity to downfall. In particular, Chaucer's poem is suffused with the language of contagion and death in connection with desire. Troilus's lovesickness mimics the progress of a viral infection. Once breached, his body performs its newly compromised identity through fever, loss of appetite, and physical disintegration. On the other hand, Chaucer depicts Boccaccio's conventional portrait of Criseyde into a elaborate paramour of a pathogen. She is characterized as the contaminant that infects male hero. In addition, Criseyde is cast as sole earthly cure of illness that Troilus suffers from. In spite of Criseyde's role as nurturer and healer, Troilus longs for his own death and feels death clutching his heart. Finally, Troilus's love toward Criseyde is doomed to death.

• 한국철학논집
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• 제29호
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• pp.7-31
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• 2010

본고의 목적은 여말선초에 나름대로 독특한 삶을 영위하며 조선조 사상계에 적지 않은 영향을 끼친 길재의 사상적 맥락을 고찰하여 재조명하는데 있다. 고려 말의 급변하는 정치상황 속에서 길재는 위화도회군 이후 이성계 등의 행동 방향을 살펴보고 쇠퇴해 가는 고려조의 명운을 근심하며 이러한 시대에 대처하는 대응방안으로 백이숙제의 절개를 고수하겠다는 입장을 분명하게 드러냈다. 여말선초에 있어서 길재가 남긴 행적은 한 신하로서의 충절(忠節)을 넘어서서, 유학적 진리에 의한 삶으로써 한 본보기가 되었다는 점에서 더 큰 의미가 있다. 조정에서 벼슬을 내렸을 때, 굳이 사퇴하며 임금을 섬기지 않은 것은 단순히 절개란 이름을 사기 위한 것이 아니다. 그것은 세상에 나오고 세상에 숨는 일을 옳게 하려는 관계이며, 유교 교리에 부합하려는 것이었다. 조선조에 들어와 역대 군왕들과 많은 유학자들이 길재의 의리정신을 추존하여 한국유학사상사에 있어서 우뚝한 위치에 올려놓은 것은 바로 이러한 점에 바탕을 둔 것이라 하겠다.

• 의정연구
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• 제25권1호
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• pp.79-106
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• 2019

지방정부 구성을 위한 한국의 지방선거에서는 종종 개별 지역에 따라 특화된 선거쟁점보다는 대통령을 둘러싼 중앙정부의 정치적인 쟁점이 선거결과에 더욱 중요한 영향을 띠어왔다. 본 연구는 2018년 지방선거를 대통령의 국정운영을 평가하는 선거로서 인식하는 유권자가 누구인지를 규명한 뒤, 지방선거를 중앙정부에 대한 견제와 평가로 보는 인식 및 대통령의 국정운영에 대한 유권자의 평가가 투표행태에 미치는 영향을 분석한다. 본 연구의 경험적 분석에 따르면, 문재인 대통령의 국정운영에 대하여 긍정적으로 인식하거나 이념적으로 중도층 또는 자유한국당을 극단적으로 좋아하거나 싫어하지 않는 유권자의 경우에 지방선거를 대통령에 대한 중간평가로 인식하는 경향이 있었다. 본 연구는 인지적 효율성을 추구하는 유권자들은 지방선거를 대통령의 국정운영을 평가하는 선거로서 인식하고 있으며, 대통령의 국정운영을 긍정적으로 인식할수록 집권여당의 후보 역시 긍정적으로 평가하고 있음을 보여준다. 지방선거는 지방자치의 핵심적인 제도이지만 지방선거에서 나타나는 중앙정치의 강한 영향은 지방자치의 독자성 및 자율성을 침해하는 문제가 될 수 있다는 점에서 지방선거 제도 및 운영에 시사하는 바가 크다고 할 수 있다.

• International Journal of Internet, Broadcasting and Communication
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• 제15권4호
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• pp.366-382
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• 2023

Among the many novels penned by Thomas Hardy, the novel "Tess of the d'Urbervilles" came into print in 1891.In this novel, Hardy portrays Tess who was born and raised in a rural middle-class family but ends up working as a laborer, trapped in a society where she is subjugated by the men's wealth, leading a life marked by loneliness and isolation. The novel presents the status of women, moral struggles, and the challenging fate that they must overcome. It highlights the powerlessness of women living in a structurally unreasonable social environment, forced to depend on the ever-changing twists of destiny. This story delves into the complexities of love between the genders, moral conflicts, and the societal criticism that must be confronted. By utilizing the life of the young woman Tess, it underscores the struggle for existence and elucidates the roles, expectations, and constraints imposed on gender during the Victorian era. This research paper aims to compare the roles of women in the Victorian era in Britain and woman in contemporary Korean society. The Victorian era featured rigid gender norms, confining women to prescribed roles. In contrast, modern Korean society blends tradition and modernity, empowering women to play vital roles and reshape societal norms. Our research explores the interplay of tradition and change, providing a comprehensive understanding of women's evolving roles in both historical and contemporary contexts. We will present our discussions regarding the enduring roles that women have faced in both periods. Furthermore, we aim to highlight the distinctive roles women are called upon to play while dealing with the swift transformations in contemporary Korean society, recognizing them as essential agents in the agents in advancing Korean society.