• 대한예방의학회:학술대회논문집
• /
• 대한예방의학회 2005년도 제57차 추계 학술대회 연제집
• /
• pp.357-357
• /
• 2005

• 한국식품영양학회지
• /
• 제11권5호
• /
• pp.550-555
• /
• 1998

The purpose of this study was to investigate the effects of n-3 polyunsaturated fatty acids(PUFA) on glucose and lipids metabolism in high-fat diet rate. Rats were randomly assigned to normal, high-fat with n-3 PUFA and high-fat dietary groups. Experiments were carried out after 5 weeks feeding with prescriptive diets following 7 hrs fasting. Body weight gains tended to be higher in high-fat fed rats than normal. Blood glucose was increased (p<0.05) by high-fat diet compared with normal diet, and decreaseed (p<0.05) to normal level by n-3 PUFA. Plasma insulin level was significcantly higher (p<0.01) in high-fat diet rats than that of normal-diet rats, and also decreased (p<0.01) by n-3 PUFA. Glucose up take of soleus muscle in vitro was decreased markedly in high-fat fed rats than normal diet rats at 0, 1, 10, and 100nM insulin concentration. Therefore insulin sensitivity and responsiveness were decreased by high-fat diet. Omega-3 PUFA made a recover(p<0.01) insulin sensitivity to almost normal level, and improved (p<0.05) insulin responsiveness in some extent. In conclusion, the results suggest that metabolic disorder of glucose and insulin resistance of skeletal muscle are caused by high-fat diet and n-3 PUFA can ameliorate metabolic disorder and insulin resistance.

• 한국산학기술학회논문지
• /
• 제9권5호
• /
• pp.1374-1385
• /
• 2008

건강관리를 위한 지역사업으로 충남 금산군에서 실시된 조사에서 주민들의 혈당수준과 이에 영향을 미치는 요인을 파악하고자 주민 1,857명을 대상으로 2006년 1월과 7월에 조사하여 분석한 결과, 공복시 혈당검사에서 전체적으로 정상군은 75.4%, 공복혈당장애군은 21.5%, 당뇨병군이 3.1%의 순이었고, 성별로는 남자에서 정상군 69.3%, 공복혈당장애군은 26.1%, 당뇨병군은 4.6%이었고, 여자는 각각 80.2%, 17.9%, 1.9%로 남자가 여자보다 공복혈당장애와 내당능장애가 높게 나타났다.

• Food Science and Biotechnology
• /
• 제16권6호
• /
• pp.879-883
• /
• 2007

This study examined whether dietary supplementation with sea tangle alters the intestinal morphology of streptozotocin-induced diabetic rats and affects the glucose absorption rate. Forty male Sprague-Dawley rats were divided into 2 groups and fed either a control (AIN76-based) diet or a sea tangle-supplemented diet. After 3 weeks, 10 rats in each group received an intramuscular injection of streptozotocin (45 mg/kg BW), and feeding was continued for 3 additional weeks. Dietary supplementation with sea tangle resulted in a lower fasting plasma glucose level compared with the control diet in diabetic rats. Scanning electron micrographs revealed serious damage to the jejunal villi of diabetic rats fed the control diet, whereas supplementation with sea tangle alleviated the damage. In a separate experiment, 20 male Sprague-Dawley rats were divided into 2 groups and fed either a control diet or a sea tangle-supplemented diet for 5 weeks, and fasted rats were subjected to in situ single-pass perfusion. The glucose absorption rate determined in the absence of digesta was decreased by 34% in the jejunum of rats fed a sea tangle diet compared with those fed a control diet. In conclusion, sea tangle supplementation lowered glucose absorption rate, altered intestinal morphology, and appeared to protect villi from damage caused by diabetes mellitus.

• Nutritional Sciences
• /
• 제3권1호
• /
• pp.11-17
• /
• 2000

This study was intended to examine whether dehydroepiandrosterone (DHEA) and dietary fat level or source could modulate glutathione utilizing detoxifying system activity and the cytosolic NADPH generation in rat liver. Male Sprague-Dawley rats were fed semipurifed diet containing either 2%(w/w) corn oil (low level of corn oil diet: 5 ca% of fat) 15% corn oil (high level of corn oil diet: 31 cal% of fat) or 13% sardine oil plus 2% corn oil(high level of fish oil diet: 31 cal% of fat) for 9 weeks. Half of the rats in each diet group were fed a diet supplemented with 0.2% DHEA (w/w). DHEA administration increased plasma total cholesterol level in low corn oil diet-fed rats. The high fish oil diet significantly decreased plasma total cholesterol level compared to the high corn oil diet. Plasma triglyceride level was not significantly changed by DHEA administration and dietary fat level and source. Fasting plasma glucose level was increased by DHEA administration and fish oil diet. Glucose 6-phosphate dehydrogenase activity in liver tissue was significantly increased by DHEA administration and high fat diet, especially fish oil diet. Malic enzyme activity in liver tissue was significantly increased by DHEA administration and high fat diet, especially fish oil diet. Malic enzyme activity in liver tissue was significantly increased by DHEA administration. DHEA suppressed the glutathione peroxidase, glutathione-dependent enzymes compared to the low corn oil diet, while fish oil diet elevated the activity of glutathione peroxidase and glutathione reductase compared to corn oil diet. These results suggest that DHEA administration and high level of corn oil diet may suppress the cellular detoxifying system activity through reduction of glutathione utilization, while the fish oil diet did not show these effects.

• 동아시아식생활학회지
• /
• 제22권3호
• /
• pp.334-340
• /
• 2012

손바닥선인장의 한 종류인 노팔(Opuntia ficus-indica (L.) Mill)을 주재료로 하여 제조한 복합물(OF)의 항당뇨 효과를 알아보기 위해 8주령 수컷 SD-rat에게 streptozotoxin을 주사하여 당뇨를 유발하고, 사료에 OF를 첨가하여 3주간 급여하였으며, 1주일 간격으로 공복 시 혈당을, 3주 후에는 당내성과 혈장 인슐린 농도를 측정하고 췌장 조직에 면역조직화학 염색을 실시하였다. 실험동물은 정상 대조군(NC), 당뇨 대조군(DC), 2% OF 급여군(OF-2), 5% OF 급여군(OF-5)으로 구분되었으며, NC와 DC는 기초식이를, OF-2와 OF-5는 기초식이에 각각 2%와 5%의 OF를 섞어서 급여하였다. 실험 개시 후 1주마다 12시간 절식시켜 꼬리정맥에서 혈액을 채취하여 공복 혈당을 측정하였다. 실험 3주 후 12시간을 절식시켜 glucose(50 mg/kg BW)를 복강주사한 다음, 30, 60, 90, 120분 경과 후에 혈당을 측정하여 당내성을 측정하였고, 심장에서 혈액을 채취하여 혈중 인슐린 함량을 분석하였다. 또한 췌장 조직에 대해 면역조직화학 염색을 실시하여 조직학적인 변화를 알아보았다. 3주간의 공복 시 혈당은 OF-5와 OF-2 모두 유의적으로 감소하였다(p<0.05). 당내성 측정 결과, OF 급여군은 DC와는 달리 혈당 농도의 변화 추이가 NC와 유사하였으며, 특히 OF-5는 OF-2에 비해서도 혈당 강하 효과가 높았던 것으로 드러났다. 췌장 조직의 면역염색에 의하면, OF의 혈당강하 기작은 췌장 Langerhans' Islet의 ${\beta}$-세포를 생성시키고, ${\beta}$-세포의 사멸을 억제시켜 인슐린의 분비를 정상화시키는 것이었으며, 이러한 결과는 혈장 인슐린 함량의 증가로 재확인할 수 있었다. 결론적으로 OF는 I형 당뇨에서 현저한 혈당 강하 효과 및 Langerhans' Islet의 ${\beta}$-세포수를 회복시켜줌으로써 I형 당뇨의 치료에 효과가 있을 것으로 사료된다.

• Biomolecules & Therapeutics
• /
• 제20권2호
• /
• pp.220-225
• /
• 2012

To develop a ginseng product possessing an efficacy for diabetes, ginseng radix ethanol extract was treated with pectinase and obtained the GINST. In the present study, we evaluate the beneficial effect of GINST on high fat diet (HFD)-induced hyperglycemia and hyperlipidemia and action mechanism(s) in ICR mice. The mice were randomly divided into five groups: regular diet group (RD), high fat diet group (HFD), HFD plus GINST at 75 mg/kg (GINST75), 150 mg/kg (GINST150), and 300 mg/kg (GINST300). Oral glucose tolerance test reveals that GINST improves the glucose tolerance after glucose challenge. Fasting plasma glucose and insulin levels were decreased by 4.3% and 4.2% in GINST75, 10.9% and 20.0% in GINST150, and 19.6% and 20.9% in GINST300 compared to those in HFD control group. Insulin resistance indices were also markedly decreased by 8.2% in GINST75, 28.7% in GINST150, and 36.4% in GINST300, compared to the HFD control group. Plasma triglyceride, total cholesterol and non-esterified fatty acid levels in the GINST300 group were decreased by 13.5%, 22.7% and 24.1%, respectively, compared to those in HFD control group. Enlarged adipocytes of HFD control group were markedly decreased in GINST-treated groups, and shrunken islets of HFD control mice were brought back to near normal shape in GINST300 group. Furthermore, GINST enhanced phosphorylation of AMP-activated protein kinase (AMPK) and glucose transporter 4 (GLUT4). In summary, GINST prevents HFD-induced hyperglycemia and hyperlipidemia through reducing insulin resistance via activating AMPK-GLUT4 pathways, and could be a potential therapeutic agent for type 2 diabetes.

• Asian-Australasian Journal of Animal Sciences
• /
• 제29권9호
• /
• pp.1294-1299
• /
• 2016

An experiment was conducted to investigate the effect of peripheral insulin treatment on appetite in chicks. Six-d-age chicks with ad libitum feeding or fasting for 3 h before injection received a subcutaneous injection of 0, 1, 3, 5, 10, or 20 IU of insulin or vehicle (saline). The results showed peripheral insulin treatment (1 to 20 IU) did not alter significantly the feed intake in chicks under either ad libitum feeding or fasting conditions within 4 h (p>0.05). Compared with the control, plasma glucose concentration was significantly decreased after insulin treatment of 3, 5, 10, and 20 IU for 4 h in chicks with ad libitum feeding (p<0.05). In fasted chicks, 10 and 20 IU insulin treatments significantly decreased the plasma glucose level for 4 h (p<0.05). Peripheral insulin treatment of 10 IU for 2 or 4 h did not significantly affect the hypothalamic genes expression of neuropeptide Y, proopiomelanocortin, corticotropin-releasing factor and insulin receptors (p>0.05). All results suggest peripheral administration of insulin has no effect on appetite in chicks.

• Asian-Australasian Journal of Animal Sciences
• /
• 제27권6호
• /
• pp.841-846
• /
• 2014

The present study was conducted to determine whether the two-step time-restricted feeding improves the fattening traits of one-step time-restricted feeding in geese. Thirty-six 8-wk-old geese were allotted into one of three groups. Group R1 (the 1-step restricted feeding group) was allowed access to feed for 2 h in the morning from 8 wk to 14 wk of age. Group R2 (the 2-step restricted feeding group) was treated as Group R1, but was additionally fed for 2 h in the afternoon from 12 wk to 14 wk of age. Group C (the control group) was fed ad libitum from 8 wk to 14 wk of age. Feed intake and body weight (BW) were recorded daily and weekly, respectively. At 14 wk of age, the blood samples were collected to determine the fasting plasma levels of glucose, triacylglycerols and uric acid before sacrifice. The results showed that daily feed intake (DFI) was lower, feed efficiency (FE) was higher in both Groups R1 and R2 than in Group C, and daily gain (DG) in Group R2 was higher than in Group R1 during the whole experimental period (p<0.05). Group R1 exhibited lower abdominal and visceral fat weights in carcass than did Group C (p<0.05), and Group R2 was in intermediate. The fasting plasma glucose levels in Group C were higher, and triacylglycerol levels in Group R1 were higher, compared with the other groups (p<0.05). It is concluded that time-restricted feeding in the fattening period not only increases FE but reduces DFI, and the additional meal during the late fattening period improves the DG without the expense of FE in geese.

• 한국임상약학회지
• /
• 제29권4호
• /
• pp.278-285
• /
• 2019

Background: Rheumatoid arthritis is highly prevalent in overweight patients with type 2 diabetes mellitus and can be reduced by physical activity via altered proinflammatory parameters. However, the association between the frequency of physical activity and the prevalence of rheumatoid arthritis in prediabetic patients remains unclear and was evaluated in this study. Methods: Utilizing the Korean National Health Insurance Sharing Service database, 58,391 adults, who met the research criteria and underwent a general medical check-up between 2009 and 2013, were selected for this study. To analyze the data, a logistic regression with a proc survey logistic procedure was used. Results: The study revealed that the cumulative rheumatoid arthritis prevalence was lower in prediabetic patients compared to that in the control group (OR, 0.64; 95% CI, 0.483-0.840; p=0.001). More frequent physical activity (≥3 days/week) was significantly associated with a reduced rheumatoid arthritis prevalence in both groups (OR, 0.28; 95% CI, 0.039-0.521; p=0.044 vs. OR, 0.15; 95% CI, 0.063-0.237; p=0.007). Additionally, a 3.8-fold higher risk of rheumatoid arthritis development was observed in prediabetic adults with less frequent baseline physical activity (≤2 days/week). Overall, in prediabetes, the prevalence of rheumatoid arthritis was associated with the frequency of physical activity and not with the fasting plasma glucose levels. Conclusion: More frequent physical activity is associated with a low risk of developing rheumatoid arthritis in prediabetic patients. Thus, further studies are needed to confirm the clinical outcomes of frequent physical activity in rheumatoid arthritis prevention and control.