• BMB Reports
• /
• v.34 no.3
• /
• pp.219-224
• /
• 2001

To identify the antioxidative peptides in the gelatin hydrolysate of bovine skin, the gelatin was hydrolyzed with serial digestions in the order of Alcalase, pronase E, and collagenase using a three-step recycling membrane reactor. The second enzymatic hydrolysate (hydrolyzed with pronase E) was composed of peptides ranging from 1.5 to 4.5 kDa, and showed the highest antioxidative activity, as determined by the thiobarbituric acid method. Three different peptides were purified from the second hydrolysate using consecutive chromatographic methods. This included gel filtration on a Sephadex G-25 column, ion-exchange chromatography on a SP-Sephadex C-25 column, and high-performance liquid chromatography on an octadecylsilane chloride column. The isolated peptides were composed of 9 or 10 amino acid residues. They are: Gly-Glu-Hyp-Gly-Pro-Hyp-Gly-Ala-Hyp (PI), Gly-ProHyp-Gly-Pro-Hyp-Gly-Pro-Hyp-Gly (PII), and Gly-ProHyp-Gly-Pro-Hyp-Gly-Pro-Hyp (PIII), as characterized by Edman degradation and fast-atom bombardment mass spectrometry. The antioxidative activities of the purified peptides were measured using the thiobarbituric acid method, and the cell viability with a methylthiazol tetrazolium assay The results showed that PII had potent antioxidative activity on peroxidation of linoleic acid. Moreover, the cell viability of cultured liver cells was significantly enhanced by the addition of the peptide. These results suggest that the purified peptide, PII, from the gelatin hydrolysate of bovine skin is a natural antioxidant, which has potent antioxidative activity.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.38 no.7
• /
• pp.727-734
• /
• 2014

This paper describes multi-scale based ductile fracture simulation using finite element (FE) damage analysis. The maximum and crack initiation loads of cracked components were predicted using proposed virtual testing method. To apply the local approach criteria for ductile fracture, stress-modified fracture strain model was adopted as the damage criteria with modified calibration technique that only requires tensile and fracture toughness test data. Element-size-dependent critical damage model is also introduced to apply the proposed ductile fracture simulation to large-scale components. The results of the simulation were compared with those of the tests on SA333 Gr. 6 full-scale pipes at $288^{\circ}C$, performed by the Battelle Memorial Institute.

• KIEE International Transactions on Power Engineering
• /
• v.3A no.1
• /
• pp.27-34
• /
• 2003

In this paper, the power conditioner composed of the stand-alone single-phase squirrel cage rotor type self-excited induction generator (SEIG) driven by prime movers such as a wind turbine and a micro gas turbine (MGT) is presented by using the steady-state circuit analysis based on the two nodal admittance approaches using the per-unit frequency in addition to a new state variable defined by the per-unit slip frequency along with its performance evaluations for the stand-alone energy utilizations. The stande-alone single-phase SEIG operating performances in unregulated voltage control loop are then evaluated on line under the conditions of the speed change transients of the prime mover and the stand-alone electrical passive load power variations with the simple theoretical analysis and the efficient computation processing procedures described in the part I of this paper. In addition, a feasuible PI controlled feedback closed-loop voltage regulation scheme of the stande-alone single-phase SEIG is designed on the basis of the static VAR compensate. (SVC) and discussed in experiment for the promising stand-alone power conditioner. The experimental operating performance results are illustrated and give good agreements with the simulation ones. The simulation and experimental results of the stand-alone single-phase SEIG with the simple SVC controller for its stabilized voltage regulation prove the practical effectiveness of the additional SVC control loop scheme including the PI controller with fast response characteristics and steady-sate performance improvements.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.37 no.5
• /
• pp.665-671
• /
• 2013

In a Korean Generation IV prototype sodium-cooled fast reactor (SFR), various types of high-temperature heat exchangers such as IHX (intermediate heat exchanger), DHX (decay heat exchanger), AHX (air heat exchanger), FHX (finned-tube sodium-to-air heat exchanger), and SG (steam generator) are to be designed and installed. In this study, the high-temperature design and integrity evaluation of the sodium-to-air heat exchanger AHX in the STELLA-1 (sodium integral effect test loop for safety simulation and assessment) test loop already installed at KAERI (Korea Atomic Energy Research Institute) and FHX in the SEFLA (sodium thermal-hydraulic experiment loop for finned-tube sodium-to-air heat exchanger) test loop to be installed at KAERI have been performed. Evaluations of creep-fatigue damage based on full 3D finite element analyses were conducted for the two heat exchangers according to the high-temperature design codes, and the integrity of the high-temperature design of the two heat exchangers was confirmed.

• Nuclear Engineering and Technology
• /
• v.52 no.2
• /
• pp.296-307
• /
• 2020

Permanent magnet sodium flowmeter (PMSF) have been used to measure the sodium flow in fast breeder reactors. Due to the effects of irradiation, thermal cycling, time lapse, etc., the magnetic flux density of the PMSF will decrease after being used in the reactor for a period of time. Therefore, it must be calibrated regularly. But some flowmeters that immersed in sodium cannot be removed for an off-line calibration, so the on-line calibration is required. However, the best online calibration accuracy of PMSF using cross-correlation analysis method was 2.0-level without considering the repeatability. In order to further improve this work, the operational principle of the transducer in PMSF is analyzed and the design principle of the transducer is proposed. The transducers were tested on the sodium flow loop to collect the experimental data. The signal characteristics are analyzed from the time and frequency domains, respectively. The cross-correlation analysis method based on biased estimation is adopted to obtain the flow rate. The verification experimental results showed that the measurement accuracy is 1.0-level when the flow velocity is above 0.5 m/s, and the measurement accuracy is 3.0-level when the flow velocity is in the range of 0.2 m/s to 0.5 m/s.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.38 no.8
• /
• pp.831-836
• /
• 2014

In this study, high temperature integrity evaluation on a pressure vessel of the expansion tank operating at elevated temperature of $510^{\circ}C$ in the sodium test facility of the SEFLA(Sodium Thermal-hydraulic Experiment Loop for Finned-tube Sodium-to-Air heat exchanger) to be constructed at KAERI has been performed. Evaluations of creep-fatigue damage based on a full 3D finite element analyses were conducted for the expansion tank according to the recent elevated temperature design codes of ASME Section III Subsection NH and French RCC-MRx. It was shown that the expansion tank maintains its integrity under the intended creep-fatigue loads. Quantitative code comparisons were conducted for the pressure vessel of austenitic stainless steel 316L.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.20 no.5
• /
• pp.71-80
• /
• 2012

To improve the $NO_X$ conversion over a SCR (selective catalytic reduction) catalyst, the DOC (diesel oxidation catalyst) is usually placed upstream of the SCR catalyst to enhance the fast SCR reaction ($4NH_3+2NO+2NO_2{\rightarrow}4N_2+6H_2O$) using equimolar amounts of NO and $NO_2$. Here, a ratio of $NO_2/NO_X$ above 50% should be avoided, because the reaction with $NO_2$ only ($4NH_3+4NO+O_2{\rightarrow}4N_2+6H_2O$) is slower than the standard SCR reaction ($4NH_3+4NO+O_2{\rightarrow}4N_2+6H_2O$). In order to accurately predict the performance characteristics of SCR catalysts, it is therefore desired to develop a more simple and reliable mathematical and kinetic models on the oxidation kinetics of nitric oxide over a DOC. In the present work, the prediction accuracy and limit of three different chemical reaction kinetics models are presented to describe the chemicophysical characteristics and conversion performance of DOCs. Steady-state experiments with DOCs mounted on a light-duty four-cylinder 2.0-L turbocharged diesel engine then are performed, using an engine-dynamometer system to calibrate the kinetic parameters such as activation energies and preexponential factors of heterogeneous reactions. The reaction kinetics for NO oxidation over Pt-based catalysts is determined in conjunction with a transient one-dimensional (1D) heterogeneous plug flow reactor (PFR) model with diesel exhaust gas temperatures in the range of 115~$525^{\circ}C$ and space velocities in the range of $(0.4{\sim}6.5){\times}10^5\;h^{-1}$.

• Journal of Energy Engineering
• /
• v.26 no.3
• /
• pp.97-104
• /
• 2017

In this study, waste tire rubbers were pyrolyzed in a lab-scale pyrolysis plant equipped with a fluidized bed reactor in a temperature ranges of $450-650^{\circ}C$. The main object of this work is to investigate the properties of pyrolysis oil with reaction temperatures and the behavior of sulfur in the products when waste polypropylene was added for co-pyrolysis. The maximum yield of oil was about 52wt.% at the reaction temperature of $456^{\circ}C$. From GC-MS analysis, the pyrolysis oils consisted mainly of limonene, toluene, xylene, styrene, trimethylbenzene, methylnaphthalenes and some heteroatom(sulfur and nitrogen)-containing compounds. The addition of waste polypropylene resulted in decrease in sulfur contents of the pyrolysis oils.

• Nuclear Engineering and Technology
• /
• v.47 no.5
• /
• pp.523-533
• /
• 2015

Three-dimensional flow phenomena in a wire-wrapped 37-pin fuel assembly mock-up of a Japanese loop-type sodium-cooled fast reactor, Monju, were investigated with a numerical analysis using a general-purpose commercial computational fluid dynamics code, CFX. Complicated and vortical flow phenomena in the wire-wrapped 37-pin fuel assembly were captured by a Reynolds-averaged Navier-Stokes flow simulation using a shear stress transport turbulence model. The main purpose of the current study is to understand the three-dimensional complex flow phenomena in a wire-wrapped fuel assembly to support the license issue for the core design. Computational fluid dynamics results show good agreement with friction factor correlation models. The secondary flow in the corner and edge subchannels is much stronger than that in an interior subchannel. The axial velocity averaged in the corner and edge subchannels is higher than that averaged in the interior subchannels. Three-dimensional multiscale vortex structures start to be formed by an interaction between secondary flows around each wire-wrapped pin. Behavior of the large-scale vortex structures in the corner and edge subchannels is closely related to the relative position between the hexagonal duct wall and the helically wrapped wire spacer. The small-scale vortex is axially developed in the interior subchannels. Furthermore, a driving force on each wire spacer surface is closely related to the relative position between the hexagonal duct wall and the wire spacer.

• Nuclear Engineering and Technology
• /
• v.27 no.3
• /
• pp.317-326
• /
• 1995

The oater level control system of the steam generator in a pressurized water reactor and its control Problems are analysed. In this work a stable control strategy Particularly during low Power operation based on the fuzzy control method is studied. The control strategy employs substitutional information using the bypass valve opening instead of incorrectly measured signal at the low How rate as the fuzzy variable of the flow rate during low power operation, and includes the flexible scale adjusting method for fast response at a large transient. A self-tuning algorithm based on the control performance and the descent method is also suggested for tuning the membership function scale. It gives a practical way to tune the controller under real operation. Simulation was carried out on the Compact Nuclear Simulator set up at Korea Atomic Energy Research Institute and its result showed the good performance of the controller and effectiveness of its tuning.