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Analysis of Labelling Efficiency According to Differences of Rotating Time in a Asan Medical Center (AMC) RBC Labelling Method (서울아산병원의 적혈구 표지 방법에서 교반 시간 차이에 따른 표지 효율의 분석)

  • Chung, Eun-Mi;Jung, Woo-Young;Ryu, Jae-Kwang;Shim, Dong-Oh;Lee, Yeong-Hee
    • The Korean Journal of Nuclear Medicine Technology
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    • v.14 no.1
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    • pp.90-93
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    • 2010
  • Purpose: In our nuclear medicine department, we suggested AMC RBC labeling method improved by modifying a part of existing modified in-vitro method to raise the efficiency of $^{99m}Tc$-RBC labeling. However, it needs to be more additional time and efforts than existing modified in-vitro method because the AMC RBC labeling method has to carry out the centrifugal separation process for 3~5 minutes. Therefore, in this study, we conducted researches to aim to maintain stable labeling effects and supplement a problem about additional time by reducing rotating time when labeling $^{99m}Tc$-RBC. Materials and Methods: This research has been conducted the object of 30 patients who examined study using $^{99m}Tc$-RBC and agreed to this research at our hospital from May 2009 to September 2009. We made 4 blood samples which consisted of ACD 1 cc along with 5 cc blood from each patient and used the AMC RBC labeling method. At this moment, each labeling efficiency was calculated by different rotating time 5 min, 10 min, 15 min, and 20 min and then we compared differences. Results: As a result, When comparing the $^{99m}Tc$-RBC labeling method efficiency by using the AMC RBC labeling method which differents from rotating time, each labeling efficiency were $92.3{\pm}5.0%$ in 5 min, $95.9{\pm}5.0%$ in 10 min, $97.4{\pm}4.9%$ in 15 min and $97.7{\pm}4.8%$ in 20 min. We analyzed differences of the labeling efficiency from change of rotating time by using an one-way ANOVA and verified that in Duncan method. There was relatively efficiency low in 5min rotating time and no statistically significant change in over. Conclusions: When comparing a existing method, the AMC RBC labeling method which goes through the centrifugal separation process again offers more favorable condition to combine RBC with $^{99m}TcO4^-$ by eliminating an plasma ingredient. When using the modified in-vitro method, we have almost 20 min to rotate to acquire stable labeling efficiency. But, when using the AMC RBC labeling method, we acquire labeling efficiency well what we want within only 10 min to rotate. Decrease of rotating time can complement the AMC RBC labeling method which goes through the centrifugal separation process again and also provide more rapid study such as G-I bleeding study due to fast labeling.

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Growth and Production of Mactra veneriformis (Bivalvia) on the Songdo Tidal Flat, West Coast of Korea (서해 송도 갯벌에서의 동죽(Mactra veneriformis: Bivalvia)의 성장과 생산)

  • 신현출;고철환
    • 한국해양학회지
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    • v.30 no.5
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    • pp.403-412
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    • 1995
  • The present study was performed to determine th growth and production of Mactra veneriformis on the Songdo tidal flat off Inchon. west coast of Korea. Monthly samples were collected from Marc 1989 to September 1990. The size distributions of shell length showed unimodal patterns from March to August 1989. The binodal pattern after September 1989 indicated a recruitment of young clams during this period. The density fluctuated irregularly, but showed a decreasing pattern over time on the whole. The density of the year class 1989 increased markedly after February 1990. The growth in shell length showed three phases during a year, a fast growth phase in spring, a slow growth phase in summer and autumn, and a lag phase in winter. The annual increment in shell length reduced with age. The flesh dry weight showed a peak in late spring just before the spawning period, and another peak in autumn. The length of annuli and the back-calculated flesh dry weight were used to describe the annual growth. The growth in shell length fitted to the von Bertalanffy model and the flesh dry weight to the Gompertz model. The absolute growth rate of the shell length reduced gradually with age. The year class 1985 had the highest L, and the yeat class 1986 the lowest L. The annual growth in weight showed a typical sigmoid curve. The growth rate was maximum at the age of 2 to 3. Year classes of 1984 and 1985 had higher values of W and higher absolute growth rates than those of 1986 and 1987. Maximum absolute growth rates of 1986 and 1987 year classes were recorded at younger ages than 1984 and 1985 year classes. The annual relative growth rates of length and weight decreased exponentially with age. Biomass in flesh dry weight increased till spring and thereafter decreased progressively. Maximum biomass was 134.6g m/SUP -2/ in August 1990. The biomass of 1989 yeat class occupied highest proportion in total biomass. Annual production in flesh dry weight from March 1989 to March 1990 was 67.9 g m/SUP -2/ year/SUP -1/. Cumulative production of 1989 year class was highest among all year classes, and that of year class 1984 was lowest. The production is high in spring and autumn, and very low in summer and winter.

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The Effects of Sight Alignment Method on Pistol Shooting Records of Security Guard (경호요원 권총사격시 조준선정렬방식과 기록변화와의 관계)

  • Lee, Sang-Chul;Shin, Seung-Cheol
    • Korean Security Journal
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    • no.14
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    • pp.367-388
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    • 2007
  • Some of individuals working at various fields of security guard are given the legal right to use a gun to cope with expected risks and crimes. Not only in Korea but also throughout the world, a pistol is being used as a device for the workers of security guard such as the army, the police and intelligence agencies to keep the law and social order. A pistol, which is regarded as the last safety measure of security guard, is easy to carry but, because of its short barrel for high portability, its hit ratio and long-range shooting performance are much lower than ordinary guns. Despite such weak points, a pistol can be a great tool because of its high portability and if the users make steady efforts to improve their skills and, for this reason, it has been being used widely until now. National agency workers' pistol shooting skills depend on several important factors, but for the fast and accurate shooting, the mot important factor is how to aim at the target. Because it takes a relatively longer time to align the sight on the target, the change of the sight alignment method is expected to improve pistol shooting skills a lot. In Korea, however, most of national agency training centers teach the basic aiming method that crosses the foresight and the backsight and aligns the crossing to the target. That is, they teach the sight alignment method for fixed targets. Some agencies in Korea and foreign countries that have used guns more frequently and longer than us are teaching different sight alignment methods as well. Representative ones of them are aiming only with the foresight and pointing shooting. These aiming methods are expected to produce good results against moving targets. While the basic sight alignment method has to cross the foresight and the backsight and then to align the crossing to the target, so takes a longer time than other aiming methods, the other two aiming methods are expected to be effective in fields where both swiftness and accuracy are require. Because domestic agencies are teaching mainly the basic sight alignment method, the present study purposed to examine the effects of different aiming methods on shooting records and ultimately to contribute to national agency workers' operation. For this purpose, we selected 20 policemen from local police commandos under the National Police Agency who were accustomed to pistol shooting and measured their pistol shooting. According to the results, the pointed shooting method showed the best record in 5m short.range shooting. As to hit ratio in 15m shooting, which is relatively long.range for pistol shooting, the basic aiming method showed the best record, but the results of statistical analysis showed that pointed shooting is most superior. Of course, the results were obtained from a group who were used to and skillful in pistol shooting, but they are considered meaningful in that all the police and national agency workers should have such high shooting skills.

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Studies on Raw Silk Cohesion for Promotion (생사의 포합향상에 관한 연구)

  • 최병희;김병호;원성희
    • Journal of Sericultural and Entomological Science
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    • v.15 no.1
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    • pp.37-48
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    • 1973
  • The purpose of this studies is to improve the cohesion of raw silk through various analyses on cocoon drying, cooking, reeling, re-reeling, and on the properties of water. Also we investigated the correlation between silk testing items which we have reached to the following results. 1. Drying of cocoon When cocoons were slowly dried with 100$^{\circ}C$, the results of cohesion became much better. On the other hand, the results were considerably decreased in case the temperature with 115$^{\circ}C$. 2. Cooking of cocoon In case of the cooking of cocoon, we found that the result of cohesion was best with incomplete cooking, that of the control was next, while in over cooking, the results were very low. Also the results of cohesion were much better when using the method of over cooking with sericin arrestive agent than that of incomplete cooking with sericin agglutinating agent. 3. Reeling of cocoon A) When the temperature of reeling bath was 25-45$^{\circ}C$, the results of cohesion test were much better, but at the temperature below 25$^{\circ}C$ or above 45$^{\circ}C$, the results became worse. B) With out the process of croissieur, the results of cohesion were too bad, but in case of croissieur more than 1cm, cohesion became better rapidly. Further more, we understood that the results of cohesion were improving slightly with longer length of croissieur. C) When the velocity of reeling was increased, the results of cohesion also improved. The best results were shown when reeling velocity was 180-220 r.p.m. But when the velocity was increased more than 220 r.p.m., the results of cohesion got worse more or less. D) When the temperature of the drying pipe in reeling machine was raised, the results of cohesion also showed a tendency to improve. 4. Re-reeling A) We could net reach a conclusion as to have correlation between the number of dipping repeat in vacuum tank and the results of cohesion before re-reeling process. B) When we used Seracol 500 as an agglutination protective agent with l/1000 to l/2000 of water, the results of cohesion test were better. C) When we used Pearl-lite as an agglutination protective agent with 1/1000 to 1/2000 of water, the results of cohesion were considerably better. D) We gained tile best results when used Cohesion Improving Chemical, A-80, with 500-1500 times diluted. 1) Results of cohesion was improved when humidity was low or temperature was high in the rereeling machine. 5. Filature water A) The water pH near the isoelectric point of protein showed the best cohesion, but the farther water pH, the worser results were obtained. B) With the increasing of M-alkalinity in filature water, the results of cohesion were worse. Above all, we understood the tendency of the results of cohesion get worse when the M-alkalinity is increased above 200 ppm. C) By increasing the total hardness of the filature water, it improved the results of cohesion. Especially, when the total hardness was above 300ppm, the results were extremely high. 6. Effects combination of each results A) The result of effects combination in filature processes with the obtained best conditions was distinctively improved. But the results could not reach in mathematicaly double effect. When reelied under worse conditions, the results of cohesion test were too bad. There was "effect limit" for the promotion. B) Generally the results of cohesion were bad when the filature conditions(the temperature, pressure and the properties of water, etc) are processed as sericin loss to be high. On the other hand, the results were very good when lower sericin loss was controlled in filature conditions. C) When filature conditions such as reeling velocity and croissieur length provide pysical cohesion ability and when raw silk dry fast during reeling and re-reeling, we found the result of cohesion was better. 7. Correlation of silk testing items. A) A negative correlation exists between the results of cohesion test and cleanness defect. Another word, the result of cohesion test was found to be worse as cleanness defect increased. B) In cleanness, cohesion has negative correlation against the number of slugs, but we could not find any correlation against long loops, loose ends. C) Cohesion has negative correlation against average neatness and low neatness defect. The better the results of neatness respectively, the better the results of cohesion found. D) There is no correlation between tenacity and the results of cohesion test, but there was high positive correlation between the results of elongation and those of cohesion test. The more elongation, the better the results of cohesion was found.

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Determination of Hydrolysis Rate Constants on Phosphamidon and Profenofos (Phosphamidon과 Profenofos의 가수분해속도 상수의 측정)

  • Min, Kyung-Jin;Ha, Young-Duck;Seo, Seol;Cha, Chun-Geun;Park, Jang-Woo;Lee, Seung-Gon
    • Journal of Food Hygiene and Safety
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    • v.15 no.2
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    • pp.144-150
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    • 2000
  • The present study was peformed to determine the hydrolysis rate constants and degradation products of phosphamidon and proffnofos by the OECD method. Hydrolysis rate constants of phosphamidon in pH 4, pH 7, and pH 9 buffer solutions at 25 and 40$^{\circ}$C were 0.0020, 0.0022, 0.0049 and 0.0040, 0.0050, 0.0150, respectively. Hydrolysis rate of phosphamidon was accelerated by temprerature change under same pH conditions, and half-life of phosphamidon in pH 9 at 40。C was 3 times faster than that at 25。C. Hydrolysis rate of phosphamidon in alkaline solution(pH 9) was 2~4 times faster than that in acidic solution(pH 4) and neutral solution(pH 7) under same temperature. Hydrolysis rate constants of profenofos in pH 4, pH 7, and pH 9 buffer solutions at 25 and 40。C were 0.0022, 0.0047, 0.0860 and 0.0035, 0.0086, 0.1245, respectively. Hydrolysis rate of profenofos was accelerated by temprerature change under same pH conditions. Hydrolysis rate of profenofos in alkaline solution(pH 9) was 15~40 times faster than in acidic solution(pH 4) and neutral solution(pH 7) under same temperature condition, and half-life of profenofos was very fast within 8 hours. The hydrolysis rate of profenofos was faster than that of phosphamidon. In order to identify hydrolysis products, the extracts of degradation products were analyzed by GC/MS. The mass spectra of hydrolysis products of phosphamidon were at m/z 153 and 149, those of the profenofos were at m/z 208 and 240, respectively. The hydrolysis products of phosphamidon were O, O-dimethyl phosphate(DMP) and N, N-diethylchloroacetamide, and those of profenofos were 4-bromo-2-chlorophenol and O-ethyl-S-propyl phosphate.

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Food Habit Patterns and Korean Food Utilization in Koreans Residing in New Zealand and Malaysia (재외 한국인의 한국음식 이용과 식생활 양상 -New Zealand 및 Malaysia 거주 한국인을 중심으로-)

  • 윤계순;우자원
    • Korean journal of food and cookery science
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    • v.14 no.5
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    • pp.566-576
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    • 1998
  • Food habits and Korean food utilization patterns were surveyed from 188 Koreans residing in New Zealand and Malaysia by using a questionnaire. Forty seven percent of the respondents answered that Korean food should be succeeded as its traditional form. Most subjects had strong pride for Korean traditional food. Kimchi was the most frequently consumed side dish followed by Chigaes (stew), broiled meat, Tubu (bean curd), broiled fish, Tangs (soup), and Noodles. While Kimchies were prepared by the respondents themselves, Doenjang and Kochujang were sent from the relatives in Korea or purchased from the market. The changes in food consumption pattern after emigration depended on the availability of food items in residing country. Korean style meal (cooked rice and side dishes) was eaten by 51% respondents for breakfast and by 78.2% for supper. Ramyon was the most frequently consumed instant food. Eating out frequency was much higher in the residents in Malaysia than the ones in New Zealand. This study showed that Koreans residing in foreign countries have the consciousness trying to succeed Korean traditional food culture, and their food consumption pattern partly depends on food availability and socio-cultural properties of the residing country.

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Clinical Characteristics of Elderly Patients with Pulmonary Tuberculosis (고령자 폐결핵에 대한 임상적 관찰)

  • Kim, Chung-Tae;Um, Hye-Suck;Lee, Hyang-Ju;Rhu, Nam-Soo;Cho, Dong-Il
    • Tuberculosis and Respiratory Diseases
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    • v.49 no.4
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    • pp.432-440
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    • 2000
  • Background : The prevalence of pulmonary tuberculosis among the elderly is increasing in Korea and in the developed countries due to the increased elderly population and their predispositions to chronic disease, poverty and decreased immunity. To define the characteristics of pulmonary tuberculosis in the elderly, we evaluated the clinical spectrum of pulmonary tuberculosis. Method : We analyzed 92 patients retrospectively that were diagnosed as active pulmonary tuberculosis over the age of 65. The analysis involved patient's profiles, clinical manifestations, coexisting diseases, diagnostic methods, anti-TB medications and their side effects, and treatment outcomes. Results : The results were as follows : - 1) The ratio of male to female was 2.1:1(62:30 cases) 2) Chief complaints were a cough (47.8%), dyspnea (40.2%), sputum (38.0%), chest pain (12.0%), anorexia (10.9%), and fever (9.8%). 3) 38 (41.3%) of cases had a past history of pulmonary tuberculosis. 4) The coexisting diseases were : -COPD, 25 cases (27.2%); pneumonia, 17 cases (18.5%); DM. 13 cases (14.1%); and malignancy, 10 cases (10.9%). 5) The positivity of Mantoux test (5 TU, PPD-S) was 82.7%. 6) Pulmonary tuberculosis was diagnosed using the following methods : sputum AFB (Acid Fast Bacillus) smear 42.4%, sputum TB (M. Tuberculosis) culture 15.2%, sputum TB PCR (Polymerase Chain Reaction) 10.9%, bronchial washing AFB smear 2.1%, chest radiology only 25.0%. 7) Locations of radiologic lesions were RULF, 50 cases; RLLF, 50 cases, mostly, then LLLF ; 26 cases were leastly involved. 8) The coexisting tuberculosis were endobronchial TB(8.7%), TB pleurisy(7.6%) miliary TB(5.4%), intestinal TB(2.2%), renal TB(1.1%) 9) The proportion of treatment regimen with 1st line drug and 2nd line drug were 92.3% and 7.6%, respectively. 10) The outcome of treatment were as follows : cured 31.5%, expired 13.0%, no return 47.8%, follow-up now 7.6%. Conclusion : The pulmonary tuberculosis in the elderly has atypical patterns with chronic coexisting diseases. Therefore, the possibility of pulmonary tuberculosis should be considered in elderly patients with pulmonary symptoms.

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Usefulness of PCR Test for M. tuberculosis for the Differentiation of Pulmonary Tuberculosis and Nontuberculous Mycobacterial Lung Disease in Patients with Smear-Positive Sputum (객담 도말 양성 환자에서 폐결핵과 비결핵 항산균 폐질환의 구별을 위한 결핵균 PCR 검사의 유용성)

  • Yu, Chang-Min;Koh, Won-Jung;Ryu, Yon Ju;Jeon, Kyeongman;Choi, Jae Chol;Kang, Eun Hae;Suh, Gee Young;Chung, Man Pyo;Kim, Hojoong;Kwon, O Jung;Lee, Jang Ho;Ki, Chang-Seok;Lee, Nam Yong
    • Tuberculosis and Respiratory Diseases
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    • v.57 no.6
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    • pp.528-534
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    • 2004
  • Background : Microscopic examination of sputum smears for acid-fast bacilli (AFB) is the most important and rapid diagnostic test for pulmonary tuberculosis. However, the AFB observed on the smear may represent either M. tuberculosis or nontuberculous mycobacteria (NTM). This study examined the clinical usefulness of a polymerase chain reaction test for M. tuberculosis (TB-PCR) for the differentiation of pulmonary tuberculosis and NTM lung disease in patients with smear-positive sputums in a tertiary hospital in Korea. Material and Methods : From January, 2003 to December, 2003, 826 AFB smear-positive and culture-positive sputum specimens were collected from 299 patients. Results : NTM were recovered from 26.6% (220/826) of the smear-positive sputum specimens and 23.4% (70/299) of the patients with smear-positive sputum. All the patients with isolated NTM had clinically significant NTM lung disease; 38 patients (54.3%) had M. avium and 26 patients (37.1%). had M. abscessus. In the patients with pulmonary tuberculosis, 78.7% of the patients (74/94) showed TB-PCR positivity, and all the patients with NTM lung disease showed negative results on the TB-PCR test (p<0.001). A positive result of the TB-PCR test on the sputum or bronchial washing fluid specimens was able to predict pulmonary tuberculosis with 88.4% sensitivity, 100% specificity, a 100% positive predictive value and a 79.7% negative predictive value for the patients with smear-positive sputum. Conclusion : The TB-PCR test for sputum specimens or bronchial washing fluid specimens could be useful for differentiating pulmonary tuberculosis and NTM lung disease for the patients with smear-positive sputum in Korea.

Comparison of Smear and Culture Positivity using NaOH Method and NALC-NaOH Method for Sputum Treatment (객담 전처리 방법에 따른 객담 항산균 도말 및 배양 양성률 비교연구)

  • Kang, Hyungseok;Sung, Nackmoon;Lee, Sunsook;Kim, Dohyung;Jeon, Doosoo;Hwang, Soohee;Min, Jinhong;Kim, Jinhee;Won, Youngsub;Park, Seungkyu
    • Tuberculosis and Respiratory Diseases
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    • v.65 no.5
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    • pp.379-384
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    • 2008
  • Background: Sputum decontamination with NALC-NaOH (N-acetyl-L-cysteine-sodium hydroxide) is known to better detect Mycobacterium tuberculosis (M. tb) by culture than that with using NaOH, which is widely used in Korean hospitals. In this report, sputum samples collected from pulmonary tuberculosis (TB) patients were treated with either NaOH or NALC-NaOH, and we compared the results of smear and culture positivity to determine whether the NALC-NaOH treatment method improves culture positivity in the sputum samples, and especially for those sputum samples that are smear negative and scanty. Methods: For each decontamination method, 436 sputum samples from pulmonary TB patients in the National Masan Tuberculosis Hospital were collected for this study. Sputum from a patient was collected two times for the first and second day of sampling time, and these samples were employed for the decontamination process by performing the 4% NaOH and NALC-2% NaOH treatment methods, respectively, for detecting M. tb by an AFB (Acid Fast Bacilli) smear and also by culture in solid Ogawa medium. Results: The NaOH and NALC-NaOH treatment methods did not significantly affect the AFB smear positivity of the sputum samples (33.0% vs 39.0%, respectively, p=0.078). However, the culture positive percents of M. tb in the Ogawa medium treated with NALC-NaOH and NaOH were 39.7% and 28.0%, respectively, which was a significantly different (p=0.0003). This difference in culture was more prominent in the sputum samples that were smear negative (the positive percents with NALC-NaOH and NaOH were 15.8% and 7.2%, respectively, p=0.0017) and scanty (NALC-NaOH and NaOH were 60.8% and 42.9%, respectively, p=0.036), but not for a smear that was 1+ or higher (p>0.05). Conclusion: NALC-NaOH treatment is better than NaOH treatment for the detection of M. tb by culture, but not by smear, and especially when the AFB smear is negative and scanty.

Gadoteridol's Signal Change according to TR, TE Parameters in T1 Image (T1영상에서 TR, TE 매개변수에 따른 Gadoteridol의 신호강도 변화)

  • Jeong, Hyun Keun;Jeong, Hyun Do;Nam, Ki Chang;Kim, Ho Chul
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.9
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    • pp.117-124
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    • 2015
  • In this paper, we introduce how to control TR, TE physical MR parameters for managing $H_1$ spin's SI(Signal Intensity) which is combined with gadolinium following administration MR agent in T1 effect for diagnostic usefulness. we used MRI phantom made with 0.5 mol Gadoteridol. This phantom was scanned by FSE sequence with different TR, TE parameters. In this study, to make T1 effect, TR was 200, 250, 300, 350, 400, 450, 500, 550, 600 msec. In addition to, TE was 6.2, 12.4, 18.6, 21.6 msec. The results were as follows ; Each RSP(Reaction Starting Point) was 100, 50, 40, 30 mmol in TE 6.2, 12.4, 18.6, 21.6 msec being irrelevant to TR. In MPSI(Max Peak Signal Intensity), 4 mmol was showed in TR 200 msec while peak signal was decreased to low concentration mol in TR 250-600 msec. In terms of RA(Reaction Area), the highest SI was TE 6.2 msec in TR 200-600msec. According to the study, we are able to recognize it is possible to control enhance rates by managing TR and TE of MR parameters; moreover, we expect that enhanced T1 image in MR clinical field can be performed in a practical way with this quantitative data.