• Asian-Australasian Journal of Animal Sciences
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• v.15 no.2
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• pp.274-278
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• 2002

A culture system for lactating rat mammary acini was evaluated, where the primary indicator of performance was lactose secretion, measured by a sensitive bioluminescence assay. Lactose secretion was reduced by half (p<0.01) over the first 6 h of culture by overnight feed withdrawal (FW) from tissue donors but was sensitive to increased glucose concentration in the culture media (p<0.001) up to 30 mM. Lactose production of cells from fed donors over the first 6 h in culture in 30 mM glucose was 8.9 fmol/cell/h - a rate calculated to be about half that in vivo. No significant difference was shown in lactose secretion by cells from fed or FW rats over 6-24 h. Lactose secretion was 3.6 fmol/cell/h by cells from fed animals in 40 mM glucose concentration media over the 6-24 h culture period. Addition of insulin to the culture media had no effect on rates of lactose secretion while addition of prolactin and hydrocortisone, with or without insulin, significantly (p<0.001) decreased lactose production over both 0-6 h and 6-24 h culture periods. Lactose synthesis in vitro was significantly enhanced by aeration of the media during collagenase digestion of mammary tissue (p<0.05). No improvement in lactose secretion was effected by shaking of cells during culture, Matrigel coating of culture dishes or change in cell density over a range up to 2.5 million cells per ml.

• The Korean Journal of Ecology
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• v.15 no.4
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• pp.377-386
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• 1992

Seasonal changes of symbiotic nitrogen-fixation activity and growth characteristics of four different natural populations of kummerowia striata were quantitatively analyzed during the growing period. the nitrogen-fixation activity of root nodules attained the maximum rates of 148, 132, 102 and 100$\muM\;C_2H_4\;\cdot\;g\;fw\;nodule^{-1}\;\cdot\;hr^{-1}$, respectively for sunny, multibranched, shade and unibranched populations at the optimum growth conditions. and the seasonal changes showed fluctuations by environmental conditions such as light, temperature, nutrient contents, water stress and plant ages, etc. The multibranched plant showed the greater amount of leaf and root nodule biomass, and the higher nitrogenase activity than the unibranched plant. the optimum conditions of leaf chloropht11 and water content of each organ indicated the active growth and the maximum fresh biomass of 4 different populations were 1.92, 1.85, 0.97 and 0.56 g $fw\cdotplant^{-1}$ for shade multibranched, sunny and unibranched populations, respectively.

• The Korean Journal of Ecology
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• v.22 no.3
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• pp.131-137
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• 1999

This study was carried out to investigate the activity of nitrogen fixation and accumulation of heavy metal and inorganic matter in Vicia amoena community at lower region in Kumho riverside, including Youngchon, Chimsangyo, Paldalgyo, Talseochon and Kumhogyo. The contents of inorganic matter and heavy metal of Kumho riverside soil increased in the down stream in each organ of the plant growing in the riverside. Generally, V. amoena community showed rapid growth of shoot and high value of Top/Root ratio. V. amoena community showed higher water content of shoot at late growth stage and higher chlorophyll content. The root nodule of V. amoena community appeared in April and increased by 0.30, 0.27, 0.24, 0.06 and 0.14 g/plant, and nitrogen fixation activity of nodule attained 20.1, 16.8, 15.4, 8.5 and 5.3 μmol·C₂H₄·g fw nodule/sup -1/·h/sup -1/ for non-contaminated area Youngchon, Chimsangyo, Paldalgyo, Talseochon and Kumhogyo, respectively, in June:. Nodule formation and nitrogen fixation activity were reduced in the down stream by the soil contamination and heavy metal accumulation and showed minimum values. at Talseochon and Paldalgyo. V. amoena showed growth adaptation against heavy metal toxicity by restricting heavy metal such as Pb, Cu, Zn, Fe from transport, and by accumulating high Ca ion in shoot, nitrogen and phosphorus in root at late growth stage than those at early one, respectively, but total heavy metal per plant showed higher values in shoot than those in root by high T/R ratio of plant growth.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.6
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• pp.701-713
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• 2003

The BEM is a highly efficient method in the sense of economical computation. However, boundary integration is not easy for the complex geometry and moving surface, e.g. a rotating blade. Thus, Kirchhoff surface is designed in an effort to overcome the difficulty resulting from complex boundary conditions. A Kirchhoff surface is a fictitious surface which envelopes acoustic sources of main concern. Acoustic sources may be distributed on each Kirchhoff surface element according to their acoustic characteristics. In this study, an axial fan is assumed to have unsteady loading noise as a dominant source. Dipole sources can be modeled to solve the FW-H equation. Acoustic field is then computed by determining Kirchhoff surface on which near-field is implemented, to analyze the effect of Kirchhoff surface on it. The optimal shape and the location of Kirchhoff surface are discussed by comparing with experimental data acquired in an anechoic chamber.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.13 no.2
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• pp.83-91
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• 2003

Pantograph design Process must be considered in terms of stability of aerodynamics and reduction of aeroacoustics. Furthermore pantograph needs to be insensible to severe circumstance condition like typhoon, tunnel, a change of season. In this paper, robust design of panhead sections is conducted based on the Taguchi's design of experiment method. In the aeroacoustic noise analysis, an acoustic analogy using the Ffowcs Williams and Hawkings(FW-H) equation is used to calculate the flow induced sound pressure level in aeroacoustics. From the near-field CFD analysis data, the far-field noise is predicted at the positions of 25 m away from Pantograph. Based on aerodynamic(CFD) and aeroacoustic(FW-H) analysis data, the optimal sizing and Positioning of panhead elements are determined using robust design optimization method. Design parameters such as thickness, length and radius are controllable factors, while outdoor air temperature and atmospheric pressure are considered as uncontrollable factors in the context of Taguchi's approach. A number of CFD simulation and aeroacoustic analysis are performed based on orthogonal arrays. In this paper, two-step optimization method is used as a parameter design procedure. It is executed using signal to noise(S/N) ratio and analysis of means(ANOM) method. So Thus, an optimal level of design parameters Is extracted to minimize the disconnection ration between contact strips and catenary system, and reduce the far-field aeroacoustic noise.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.26 no.6_spc
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• pp.705-713
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• 2016

Underwater noise produced by ships has been becoming an increasing issue. A dominantly contributing noise source is a ship propeller. Therefore, it is important to predict the propeller noise at the propeller design stages. This study applied the acoustic analogy based on Ffowcs Williams equation for the prediction of the marine propeller BPF noise. A marine propeller BPF noise is investigated experimentally as well as numerically. Propeller BPF noise measurement and propeller cavitation observation tests are performed in the KRISO medium size cavitation tunnel. Numerical prediction schemes of marine propeller BPF noise are presented together with the noise measurement method. Propeller BPF noise predictions and experiments are performed under the various propeller operating conditions including non-cavitating and caveating conditions. Numerical and experimental results are compared and analyzed. It is shown that numerical prediction results are generally in good agreement with the measured data.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2001.11b
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• pp.1235-1241
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• 2001

Pantograph design process must be considered in terms of stability of aerodynamics and reduction of aeroacoustics. Furthermore Pantograph needs to be insensible to severe circumstance condition like typhoon, tunnel, a change of season. In this paper, robust design of panhead sections is conducted based on the Taguchi's design of experiment method. In the aeroacoustic noise analysis, an acoustic analogy using the Ffowcs Williams and Hawkings (FW-H) equation is used to calculate the flow induced sound pressure level. From the near-field CFD analysis data, the far-field noise is predicted at the positions of 25m away from panhead contact strips. Based on aerodynamic (CFD) and aeroacoustic (FW-H) analysis data, the optimal sizing and positioning ofpanhead elements are determined using robust design optimization method. Design parameters such as thickness, length and radius are controllable factors, while outdoor air temperature and atmospheric pressure are considered as uncontrollable factors in the context of Taguchi's approach. A number of CFD simulation and aeroacoustic analysis are performed based on orthogonal arrays. Using a parameter design procedure associated with signal-to-noise (SIN) ratio and sensitivity analysis, an optimal level of design parameters are extracted to minimize the disconnection ratio between contact strips and catenary system, and reduce the far-field aeroacoustic noise.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2000.11a
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• pp.759-764
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• 2000

The accurate prediction of aeroacoustic analysis is necessary for designers to control and reduce airflow-induced sound pressure levels in high speed rotating DVD drives. This paper focuses on the numerical prediction of airflow-induced sound in DVD drives. Computational fluid dynamics(CFD) is first conducted to evaluate flow field characteristics due to the high-speed disk rotation, and to support the acoustic analysis. The acoustic analogy based on Ffowcs Williams-Hawkings(FW-H) equation is adopted to predict aeroacoustic noise patterns. The integral solution for quadrupole volume source is included to identify the turbulence noise generated inside the DVD tray. The strength of sound pressure level with respect to rotating speed is discussed to meet upfront demand on the high fidelity product development. The present study also focuses on the noise directivity and examines how much the sound noise is sensitive to change in rotating speed. Near-field noise is strongly affected by the flow field characteristic, which is caused by the complex shape of the tray. For a mid-field, the quadrupole noise play as a counterpart of thickness noise or loading noise, so it generates different sound noise patterns compared with those in the near field.

• The Journal of the Acoustical Society of Korea
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• v.38 no.3
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• pp.247-255
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• 2019

In this study, to predict the noise of a submarine propeller which is going to become bigger and faster, the non - cavitating propeller noise was predicted based on the numerical analysis which considering the interaction of the hull - appendages - propeller. In order to predict the radiated noise of the propeller, the flow field for the entire region of hull-appendages-propeller was computed by CFD (Computational Fluid Dynamics). And the noise for the thickness noise and the load noise was numerically predicted using FW-H (Ffwocs Williams-Hawkings) acoustic analogy. Numerical noise prediction results were verified by model tests and showed good agreement with the measurement results in predicting total noise level and low frequency noise.

• Ocean Systems Engineering
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• v.13 no.2
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• pp.147-172
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• 2023

This study investigates the influence of loading and inflow conditions on tidal turbine performance from a hydrodynamic and hydroacoustic point of view. A boundary element method is utilized for the former to investigate turbine performance at various loading conditions under zero/non-zero yaw inflow. The boundary element method is selected as it has been selected, tested, and validated to be computationally efficient and accurate for marine hydrodynamic problems. Once the hydrodynamic solutions are obtained, such as the time-dependent surface pressures and periodic motion of the turbine blade, they are taken as the known noise sources for the subsequence hydroacoustic analysis based on the Ffowcs Williams-Hawkings formulation given in a form proposed by Farassat. This formulation is coupled with the boundary element method to fully consider the three-dimensional shape of the turbine and the speed of sound in the acoustic analysis. For validations, a model turbine is taken from a reference paper, and the comparison between numerical predictions and experimental data reveals satisfactory agreement in hydrodynamic performance. Importantly, this study shows that the noise patterns and sound pressure levels at both the near- and far-field are affected by different loading conditions and sensitive to the inclination imposed in the incoming flow.