• Clinical and Experimental Reproductive Medicine
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• 제38권2호
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• pp.82-86
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• 2011

Objective: To evaluate sperm nuclear DNA fragmentation and chromatin structure after 18 hours' incubation at room temperature. Methods: Twenty-eight male partners who participating IVF treatment were prospectively included in this study. Ejaculated sperm count and motility were assessed. The sperm was then immediately processed by the conventional swim-up method. After utilization of some of the sample for routine clinical use, the remainder of each of the samples was divided into two aliquots. One aliquot was immediately assessed for sperm nuclear DNA fragmentation (TUNEL assay) and chromatin structure (toluidine blue [TB] staining). The other aliquot was incubated at room temperature for 18 hours and then assessed by two methods. Only dark-TB sperms were considered as having abnormal chromatin structure. Data before and after extended incubation were compared using a paired Student's $t$-test. Results: Before and after extended culture, nuclear DNA fragmentation assessed by TUNEL was $4.9{\pm}4.7%$ and $7.0{\pm}6.4%$, respectively ($p$=0.008). The proportion of abnormal chromatin structure (dark-TB sperm) was $8.2{\pm}5.6%$ and $10.3{\pm}6.5%$ ($p$ <0.001), before and after incubation, respectively. Conclusion: After 18 hours' incubation at room temperature, sperm nuclear DNA and chromatin structure were significantly affected. The IVF practitioner should bear this information in mind when performing delayed insemination, especially for $in$ $vitro$ maturation cycles.

• Clinical and Experimental Reproductive Medicine
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• 제46권2호
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• pp.67-75
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• 2019

Objective: Sperm cryopreservation has been widely used in assisted reproductive technology, as it offers great potential for the treatment of some types of male infertility. However, cryopreservation may result in changes in membrane lipid composition and acrosome status, as well as reductions in sperm motility and viability. This study aimed to evaluate sperm DNA fragmentation damage caused by conventional freezing using the sperm chromatin dispersion test. Methods: In total, 120 fresh human semen samples were frozen by conventional methods, using SpermFreeze Solution as a cryoprotectant. Routine semen analysis and a Halosperm test (using the Halosperm kit) were performed on each sample before freezing and after thawing. Semen parameters and sperm DNA fragmentation were compared between these groups. Results: There was a significant decrease in sperm progressive motility, viability, and normal morphology after conventional freezing (32.78%, 79.58%, and 3.87% vs. 16%, 55.99%, and 2.55%, respectively). The sperm head, midpiece, and tail defect rate increased slightly after freezing. Furthermore, the DNA fragmentation index (DFI) was significantly higher after thawing than before freezing (19.21% prior to freezing vs. 22.23% after thawing). Significant increases in the DFI after cryopreservation were observed in samples with both normal and abnormal motility and morphology, as well as in those with normal viability. Conclusion: Conventional freezing seems to damage some sperm parameters, in particular causing a reduction in sperm DNA integrity.

• Clinical and Experimental Reproductive Medicine
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• 제46권1호
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• pp.14-21
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• 2019

Objective: The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to reproductive failure, suggesting that it should be evaluated as part of male infertility assessments. To compare the ability of the five most widely utilized methodologies of measuring DNA fragmentation to predict male infertility and reactive oxygen species by Oxisperm kit assay. Methods: In this case-control study, which received ethical committee approval, the participants were divided into fertile and infertile groups (50 patients in each group). Results: The alkaline comet test showed the best ability to predict male infertility, followed by the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay, the sperm chromatin dispersion (SCD) test, and the sperm chromatin structure assay (SCSA), while the neutral comet test had no predictive power. For our patient population, the projected cut-off point for the DNA fragmentation index was 22.08% using the TUNEL assay, 19.90% using SCSA, 24.74% using the SCD test, 48.47% using the alkaline comet test, and 36.37% using the neutral comet test. Significant correlations were found between the results of the SCD test and those obtained using SCSA and TUNEL (r = 0.70 and r = 0.68, respectively; p< 0.001), and a statistically significant correlation was also found between the results of SCSA and the TUNEL assay (r = 0.77, p< 0.001). Likewise, the results of the alkaline comet test showed significant correlations with those of the SCD, SCSA, and TUNEL tests (r = 0.59, r = 0.57, and r = 0.72, respectively; p< 0.001). Conclusion: The TUNEL assay, SCSA, SCD, and the alkaline comet test were effective for distinguishing between fertile and infertile patients, and the alkaline comet test was the best predictor of male infertility.

• 화약ㆍ발파
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• 제41권4호
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• pp.41-50
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• 2023

본 연구에서는 굴착 물량 증대와 발파공해 저감을 위해서 전자뇌관을 이용한 수직방향 더블 데크 공법을 적용하였다. 더블 데크 공법은 상부 데크 발파 후 자유면이 완전히 형성되지 않은 상태에서 하부 데크 발파가 진행되면 발파 효율이 감소할 가능성이 있다. 이와 같은 이유로 더블 데크 공법은 데크 단차에 따라서 발파 효율이 달라진다. 따라서 본 연구에서는 홀 단차의 1~5배를 적용한 4가지 데크 단차를 제안하였고, 발파 후 파쇄도 분석을 통해 발파 효율을 평가하였다. 파쇄도 평가 결과 패턴 4(데크 단차=홀 단차×5)의 파쇄도가 가장 좋았으며, 패턴 3(데크 단차=홀 단차×3) 이상에서 파쇄효율이 큰 폭으로 증가하는 것을 확인할 수 있었다. 이에 따라 수직방향 더블 데크 발파 시 데크 단차는 최소 홀 단차의 3배 이상이 되어야 효율적인 발파 효과를 얻을 수 있다고 분석되었다.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제5권1호
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• pp.10-20
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• 2024

The study investigated species diversity, relative abundance, and decline of flying insects and plants within a fragmented forest in the Federal University of Technology Akure (FUTA), Ondo State, Nigeria. It is known that habitat fragmentation can reduce biodiversity. Thus, it is important to perform comprehensive assessments to understand implications of the habitat fragmentation for flora and fauna. Species richness and abundance of flying insects and plants across fragmented forest patches were quantified using field surveys and taxonomic identification. This study revealed shifts in species diversity, with fragmented areas exhibiting reduced biodiversity compared to contiguous forest ecosystems. Flying insects crucial for ecosystem functioning and pollination services demonstrated decreased species richness and relative abundance within fragmented habitats. This decline was attributed to habitat loss, altered microclimates, and limited movement pathways known to hinder insect dispersal. Similarly, plant species richness and abundance showed decline in fragmented forest due to disrupted mutualistic interactions with pollinators, altered nutrient cycling, and increased competition among plant species. This study underscores the importance of maintaining intact forest habitats to sustain healthy ecosystems and preserve biodiversity. Effective conservation strategies should focus on habitat connectivity, reforestation efforts, and protection of essential ecological corridors to mitigate effects of fragmentation. In conclusion, this investigation provides empirical evidence for effects of habitat fragmentation on flying insects and plants in a forest ecosystem in FUTA Akure, Nigeria. Findings emphasize an urgency of adopting conservation measures to safeguard these invaluable components of biodiversity and ecosystem stability in the face of ongoing habitat loss and fragmentation.

• 한국지리정보학회지
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• 제9권4호
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• pp.81-94
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• 2006

본 연구에서는 도암댐 유역에서의 산림파편화에 따른 수(水)환경 영향을 평가하였다. 이를 위해서 Soil and Water Assessment Tool (SWAT) 모형을 이용하였다. 도암댐 유역에서 산림파편화에 따른 수환경 영향만을 평가하기 위해서는 먼저 기상변화에 따른 영향을 배제시켜야 한다. 1985년과 2000년 강수량 분석결과 계절별 강수량에 상당히 많은 차이가 나타나, 본 연구에서는 1985년 기상자료를 이용하여 산림파편화에 따른 수환경 영향을 평가하였다. 산림 파편화에 따른 영향은 시간적 공간적으로 변화하기 때문에 본 연구에서는 수환경의 시 공간적 영향을 분석하였다. 도암댐 수계중 산림파편화가 가장 많이 발생한 소유역에서는 산림파편화로 인하여 유출량이 겨울철, 봄철, 여름철, 가을철 각각 $8,366m^3/month$, $72,763m^3/month$, $149,901m^3/month$, 그리고 $107,109m^3/month$ 증가하였다. 이렇게 증가된 유출량으로 인하여 상당한 양의 토양유실이 발생하여 하천의 부영양화 등을 초래한 것으로 판단된다. 이러한 토양유실로 인하여 가장 많은 산림파편화가 일어난 소유역에서 겨울철, 봄철, 여름철, 그리고 가을철, 유사농도가 각각 5.448mg/L, 13.354mg/L, 20.680mg/L, 그리고 24.680mg/L 증가하였다. 봄철 유사농도가 많이 증가한 이유는 산림지역과는 달리 농경지에서의 봄철 융설로 인한 토양유실이 많이 발생하였기 때문이다. 여름철과 가을철 유사농도가 증가한 이유 또한 과거 산림지역이었던 곳이 산림파편화로 인하여 상당부분 농경지로 전환되었으며, 이 지역에서의 영농에 따른 토양유실 증가로 유사농도가 증가한 것으로 판단된다. 본 연구의 결과를 종합해 보면 홍수조절, 토양유실 저감, 수질 개선과 같은 산림 고유의 기능은 유역내 산림파편화로 인하여 매우 빨리 손실될 수 있다. 따라서 도암댐 유역 내에서 산림 파편화에 따른 부정적 영향을 최소화시키기 위해서는 복합적인 토지이용계획을 수립하여 이행되어야 한다.

• 대한화학회지
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• 제51권2호
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• pp.160-164
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• 2007

고체상 표면에서 퓨록산 화합물의 박막생성을 위하여 클로로퓨록산 2와 butyl 및 benzyl amine과 반응시킨 아미노퓨록산 3,4를 합성하고 이들 아미노퓨록산 화합물을 질량분석기에서 전자빔에 의한 분해반응을 분석하여 알카인의 생성에 대한 효율성을 살펴보았다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 하계종합학술대회 논문집(1)
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• pp.317-320
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• 2004

In this paper, it is analyzed a performance improvement of ACL Packet using turbo code scheme in bluetooth system. In order for segment to improve the transfer capability, the transmission of messages have been simulated using a fragmentation that begins with the total package and incremental fragmentation for each layer using the TCP to define the resultant packet size and the level of fragmentation for each proceeding layer. This turbo code scheme decreases transmission time of L2CAP baseband packets by sending packets. From the results, we were able to obtain packet transmission time, optimal TCP packet size, ACL(DM packet) in AWGN.

• 정보보호학회논문지
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• 제27권4호
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• pp.831-841
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• 2017

본 논문에서는 콘텐츠 중심 네트워크(CCN, Content-Centric Networking)를 위한 안전한 패킷 단편화(fragmentation) 기술을 제안한다. 제안하는 기술은 패킷조각(fragment)들에 대한 불법적인 위변조 가능성을 차단하고, 패킷조각 수신 시 높은 확률로 즉각적인 신뢰성 검증을 제공함으로써 검증 지연을 악용한 DoS 공격으로부터 높은 안전성을 제공한다. 본 논문의 성능 분석 결과는 약간의 부하 증가만으로 제안하는 기술이 기존 기술보다 훨씬 더 높은 안전성을 제공함을 보여준다.

• Bulletin of the Korean Chemical Society
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• 제25권2호
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• pp.260-262
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• 2004

In agreement with the results of previous MP2 calculations by Sauers, B3LYP, CASSCF, and CASPT2 calculations on the parent 2-carbena-1,3-dioxolane show that it fragments to ethylene plus $CO_2$ by a concerted pathway with only a small energy barrier. Not only is fragmentation via stepwise C-O bond cleavage computed to be a much higher energy pathway, but the singlet diradical that would be an intermediate along such a reaction path is not even computed to be a local minimum on the potential energy surface.