• Title/Summary/Keyword: FP activity

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Preparation and Keeping Quality of Proteolytic Enzymes from Seafood rocessing Wastes (어류가공 부산물로부터 단백질분해 효소제의 조제 및 보관안정성)

  • KIM Jin Soo;HEU Min Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.4
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    • pp.259-268
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    • 2004
  • Keeping qualities of crude proteases (CP) and fractionated proteases (FP) sedimenting with $30\~80{\%}$ ammonium sulfate from four kinds of fish viscera as a seafood processing waste were examined. Azocaseinolytic activlties (pH 6 and 8) of CP from anchovy (Engraulis japonica), mackerel (Scomber japonicus), bastard flatfish (Pararlichthys olivaceus) and red sea bream (Chysorphys major) were stable without activity loss at $4^{\circ}C$ for 7 months. Activities of NaCP (CP containing $30{\%}$ sodium chloride) on azocasein were approximately $30{\%}$ lower than those of CP. FP activities Increased 3.4-16.1 folds compared to those of CP and NaCP Powdered crude protease (PCP) and fractionated and powdered protease (FPP) containing various sugars (lactose, sucrose, glucose and dextrin) were prepared by freeze drying. Activities of PCP and FPP containing sucrose were higher and more stable than those of PCP and FPP containing other sugars at $30^{\circ}C$ for whole keeping periods. PCP and FPP from mackerel viscera showed the highest proteolytic activity among four kind of fish vlsceras. The Optimum conditions and stabilities of FPP from mackerel viscera were pH 9 and $50^{\circ}C$, and pH 5-10 and $20-45^{\circ}C$, respectively. The results of this study suggest that FPP from seafood processing waste may be used as processing aids.

Effects of Combined Breathing Exercise and Neuromuscular Electrical Stimulation on Patients with Severe COPD (중증 만성폐쇄성폐질환 환자를 위한 복합호흡운동과 신경근전기자극의 효과)

  • Kang, Jeong-Il;Park, Jun-Su;Jeong, Dae-Keun
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.7
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    • pp.539-548
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    • 2019
  • This study was designed to examine the effects of complex breathing exercise and neuromuscular electrical stimulation of Quadriceps Femoris muscle on pulmonary function and cerebral cortex activity in patients with severe chronic obstructive pulmonary disease. After collecting samples from 20 patients with severe chronic obstructive pulmonary disease aged 60 to 80, 10 patients each were randomly placed in an experimental group and a control group. The experimental group conducted complex breathing exercise and neuromuscular electrical stimulation of Quadriceps Femoris muscle, and the control group only conducted complex breathing exercise. As a pretest, pulmonary function and cerebral cortex activity were measured. The intervention program was applied to each group for 30 minutes, once a day, for 4 days a week, for 6 weeks, and the posttest was carried out the same way as the pretest. As a result, both groups showed significant differences in FEV1.0(Forced Expiratory Volume in One Second)(p<.001)(p<.05), and there were significant differences between the groups as well(p<.05). When comparing alpha waves in each domain of cerebral cortex, both of the experimental and control groups showed significant differences in Fp1, Fp2, F3 and F4 domains (p<.01)(p<.05). During the 6-week experiment, complex breathing exercise and neuromuscular electrical stimulation of Quadriceps Femoris muscle improved pulmonary function of patients with severe chronic obstructive pulmonary disease, and in relation to cerebral cortex activity, a positive breathing change was found due to the increase of alpha waves in the forehead domain. Therefore, it is considered that applying neuromuscular electrical stimulation of Quadriceps Femoris muscle to patients with severe chronic obstructive pulmonary disease additionally along with complex breathing exercise will bring a better therapeutic effect.

Comparison of brain wave values in emotional analysis using video (영상을 이용한 감정분석에서의 뇌파 수치 비교)

  • Jae-Hyun Jo;Sang-Sik Lee;Jee-Hun Jang;Jin-Hyoung Jeong
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.16 no.6
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    • pp.519-525
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    • 2023
  • The human brain constantly emits electrical impulses, which is called brain waves, and brain waves can be defined as the electrical activity of the brain generated by the flow of ions generated by the biochemical interaction of brain cells. There is a study that emotion is one of the factors that can cause stress. Brain waves are the most used in the study of emotions. This paper is a study on whether emotions affect stress, and showed two images of fear and joy to four experimenters and divided them into three stages before, during, and after watching. As a measurement tool, brain waves at the positions of Fp1 and Fp2 were measured using the NeuroBrain System, a system that can automate brain wave measurement, analysis, brain wave reinforcement, and suppression training with remote control. After obtaining the brain wave data for each emotion, the average value was calculated and the study was conducted. As for the frequency related to stress, the values of Alpha and SMR, Low Beta, and High Beta were analyzed. Brainwave analysis affects stress depending on the emotional state, and "fear" emotions cause anxiety by raising Beta levels, resulting in higher Mind Stress levels, while "joy" emotions lower Beta levels, resulting in a significant drop in Mind Stress.

Hydrolysis of Paper Mill Sludge Using an Improved Enzyme System

  • Lin Jianqiang;Lee, Sang-Mok;Koo, Yoon-Mo
    • Journal of Microbiology and Biotechnology
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    • v.11 no.3
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    • pp.362-368
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    • 2001
  • The effects of water soluble materials in paper mill sludge on cellulase and $\beta$-glucosidase activities were studied while the optimization of enzyme system for hydrolysis of the paper mill sludge for production of glucose was made. Water soluble materials in the paper mill sludge showed stimulatory effect on carboxymethyl cellulose (CMC) activity, inhibitory effect on filter paper (FP) activity, and no effect on avicelase and $\beta$-glucosidase activities. CMC and ${\beta}$-glucosidase activities at 5 and 10, 5 or 10 and 10, and 10 and 10 U/ml were optimal for hydrolysis of 5, 10, and 20% of the paper mill sludge, respectively.

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Effect of Grass Lipids and Long Chain Fatty Acids on Cellulose Digestion by Pure Cultures of Rumen Anaerobic Fungi, Piromyces rhizinflata B157 and Orpinomyces joyonii SG4

  • Lee, S.S.;Ha, J.K.;Kim, K.H.;Cheng, K.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.1
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    • pp.23-30
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    • 2000
  • The effects of grass lipids and long chain fatty acids (LCFA; palmitic, stearic and oleic acids), at low concentrations (0.001~0.02%), on the growth and enzyme activity of two strains of anaerobic fungi, monocentric strain Piromyces rhizinflata B157 and polycentric strain Orpinomyces joyonii SG4, were investigated. The addition of grass lipids to the medium significantly (p<0.05) decreased filter paper (FP) cellulose digestion, cellulase activity and fungal growth compared to control treatment. However, LCFA did not have any significant inhibitory effects on fungal growth and enzyme activity, which, however, were significantly (p<0.05) stimulated by the addition of oleic acid as have been observed in rumen bacteria and protozoa. This is the first report to our knowledge on the effects of LCFA on the rumen anaerobic fungi. Continued work is needed to identify the mode of action of LCFA in different fungal strains and to verify whether these microorganisms have ability to hydrogenate unsaturated fatty acids to saturated fatty acids.

Standardization of Pancreatin (판크레아틴의 규격 표준화 연구)

  • Shin, Ji-Eun;Yoon, Hae-Kyung;Kim, Dong-Hyun
    • Journal of Pharmaceutical Investigation
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    • v.33 no.4
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    • pp.273-279
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    • 2003
  • Pancreatin is a enzyme mixture breaking down carbohydrates, proteins and lipids. Most pancreatin used in Korea is imported from foreign countries. However, guideline of each country for pancreatin produced from each country is different. Therefore, guideline for pancreatin imported from several countries, such as Europe, Japan and America, it is standardized to control its quality. Assay of enzyme activity for pancreatin in KP is similar to tat in JP, but it is significantly different from those in FP ad in USP. We measured pancreatin digestive activities of 17 commercial products. Activity assay of digestive enzymes, starch- and lipid-digestive enzymes, for pancreatin by KP method (including JP) was difficult compared to those by FIP ad USP methods. Particularly, activity assays of starch- and lipid-digestive enzymes by KP method were mistakable, ad varied in diluted samples than those by FIP. However, activity assay of protein-digestive enzyme by KP method was similar to that by FIP. Starch-digestive enzyme activities of 17 commercial pancreatins by KP method were lower 0.079-fold compared to those by FIP method. Their protein-digestive enzyme activities by KP method were higher 75.7-fold than those by FIP method. Their lipid-digestive enzyme activities by KP method were lower 0.234-fold compared to those by FIP method.

The Effects of the Otago Exercise Combined with Action Observation Training on Brain Activity of the Elderly

  • Kim, Jung-hee;Kim, Eun kyong;Lee, Byounghee
    • Journal of Korean Physical Therapy Science
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    • v.26 no.3
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    • pp.1-7
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    • 2019
  • Purpose: The purpose of this study was to investigate the effects of the Otago exercise combined with action observation training on changes of the brain activity of the elderly. Design: Randomized controlled trial. Methods: Thirty elderly women in the experiment were included. Participants were randomly assigned to the Otago combined with action observation training group, the Otago exercise group, and the control group (10 in each group). The Otago combined with action observation training group and the Otago exercise group performed the strength and balance exercises of the Otago exercise program for 50 minutes three times a week for 12 weeks. The Otago combined with action observation training group underwent additional action observation training for the Otago movement for 20 minutes three times a week. No intervention was performed in the control group. PolyG-1 (LAXTHA Inc., Daejeon, Korea) was used to measure the changes in the brain activity following intervention. One-way analysis of variance was used to compare the effects among the groups and a post-hoc test was performed. Results: The relative mu rhythms in the F3, C3, and C4 regions were significantly increased in the Otago combined with action observation training group. Relative beta wave activity in the Fp1, F3, F3, and C3 regions was significantly increased in the Otago combined with action observation training group (p<0.05). Conclusion: The results indicated that the Otago exercise combined with action observation training was effective for promoting the brain activity of the elderly.

The Influence of Negative Emotion to Cortical Activity Induced by Auditory Verbal Imagery in Patients with Schizophrenia (정신분열병 환자에서 부정적 감정이 청각적 언어상상에 의해 유발된 대뇌 피질 활성에 미치는 영향)

  • Lee, Hong-Shick;Kim, Ji-Woong;Kim, Yully
    • Korean Journal of Biological Psychiatry
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    • v.7 no.2
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    • pp.174-179
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    • 2000
  • Objectives : Cognitive psychological models propose that auditory hallucinations arise from a problem with monitoring one's auditory verbal imagery. Most auditory hallucinations are derogatory in content and accompany negative emotions. If auditory verbal imagery plays an critical role in the pathogenesis of auditory hallucination, it must be influenced by negative emotions. This study was aimed at understanding the influence of negative emotions on the development of hallucinations by investigating the way by which negative emotions have influence on cortical activity induced by auditory verbal imagery. Methods : For both normal subjects and patients with schizophrenia, quantitative electroencephalography(Q-EEG) was applied during the auditory verbal imagery tasks using a two word list. The one word list accompanied negative emotion and the other accompanied neutral emotions. The difference of EEG activity between two tasks was compared by paired t-test. We also compare the difference of the influence of negative emotions between normal subjects and patients with schizophrenia Results : In normal subjects, amplitude of beta wave was increased in temporal area such as TCP1, and, the amplitude of theta frequency wave was decreased in right hemisphere such as FP2, F4, C4, CP2, P4. But, in the schizophrenia group, there were no significant differences. Conclusion : These results may suggest that auditory verbal imagery with negative emotion requires more activation in left temporal area, but, appropriate activation may not achieved in schizophrenia patients. So, the possibility that the resultant disturbance of verbal self monitoring may be related to auditory hallucination is suggested in this study.

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Design of Test-Effort Estimation Model (소프트웨어 시험노력 추정 모델의 설계)

  • Kim, Hankyoung
    • Journal of Internet Computing and Services
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    • v.14 no.1
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    • pp.23-30
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    • 2013
  • Test effort estimated so far is as a by-product of the development effort estimation activity which is based on the FP, UCP, COCOMO model, or calculated data from the project knowledge base which is containing test effort information for the test phase on software development life cycle. In this paper, test effort estimation model and calculating procedures are suggested, which is independent from software development effort estimation model. Generally test efforts is depends on the number and the complexity of test cases, and also maturity of test organization that performs test activities, such as integration test, system test, acceptance test and so on. The estimated results with the suggested test effort estimation model has deviation of 4.7% compare to the corresponding test efforts generated by the development effort estimationprocedures. The suggesting model will be accurate more and more with refinements of coefficients which reflect the technical and environmental maturity level of test organization, and also including the software complexity level of projects.

Evaluation of Fuel Cladding Failures from the Fission Product Activities in the Reactor Coolant (원자로 냉가수내의 핵분열생성물 방사에 의한 핵연료피복관 파손 평가)

  • Ho Ju Moon;Sung Ki Chae
    • Nuclear Engineering and Technology
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    • v.16 no.3
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    • pp.169-179
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    • 1984
  • An efficient procedure of evaluating the fuel cladding failures occurring in the normal operations of typical PWR's has been investigated through the analysis of fission product(FP) activities in the reactor coolant using an analytical model, FIPREL code. Performed by this code is an extensive study on the sensivities of FP activities to such physical parameters as enrichment, turnup, and operation temperature of failed fuel rod as well as the effective failure size quantified in terms of the magnitude of gap release coefficient. The results of study are generally in agreement with those by PROFIP method. In the presence of tramp uranium the portion of activities released from failed rod is separated by an iterative calculation based on the activity ratios of fission nuclides chemically more stable than iodines. Obtained are the linear power density and the number of failed rods, the effective failure size, and the mass of tramp uranium. The operation experiences of 4 cycles of Kori Unit 1 are analyzed and the results show that the model is highly reliable for the survey and evaluation of fuel rod conditions during reactor operations.

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