• Fisheries and Aquatic Sciences
• /
• 제5권4호
• /
• pp.311-313
• /
• 2002

Proteolytic activity of live Uronema manum was analyzed by fluorescence polarization (FP) technique. Protease activity was measured by a decrease in FP value using fluorescein isothiocynate (FITC)-casein as a protein substrate. The results demonstrated an inverse linear relationship between fluorescence polarization (FP) values and live ciliate concentration over the range $1\times10^4\;to\;2\times10^5$ cells/well. However, the FP values of $10-10^3$ live parasites were not different significantly from that of control. Time-dependent decrease in FP value was shown in the wells containing live U. marinum. In the present study, FP assay had the benefit to provide measurements of substrate hydrolysis by live parasites in real-time, and did not require separations, precipitations, or transfers of reaction mixture.

• Electronics and Telecommunications Trends
• /
• 제24권2호
• /
• pp.84-96
• /
• 2009

유럽연합은 1990년대에 접어들면서 미국과의 경제 격차가 점차 확대되자 미국을 따라잡기 위해 리스본 전략을 채택하였다. 이 전략의 목표는 유럽의 정보화를 통한 성장, 고용, 사회적 참여로 요약할 수 있다. 리스본 전략에서 제시된 정보화 모토는 정책과 연구개발을 2개의 기둥으로 하고, 이 두 영역간의 긴밀한 상호협조를 통해 유럽정보화의 시너지를 창출하자는 것이었다. 정책 부문은 리스본 전략에서 출발하여 신 리스본 전략, i2010 전략으로 발전되어 왔으며, 이에 맞추어 연구개발 부문은 FP5, FP6으로 발전되어 오다가 현재는 FP7이 시행되고 있다. 본 고에서는 FP7의 ICT 연구프로그램에서 제안하는 도전과제와 미래기술전략을 소개하는 한편, 그 정책적 시사점을 도출하였다. FP7 ICT 연구프로그램은 유럽이 차세대 ICT 및 그 사용에 있어서 세계 선도 그룹에 들기 위해 극복해야 할 7가지 도전과제를 제안하고 있다. 이들 도전 과제들은 크게 업계의 기술 목표 및 사회경제적 목표 달성을 위한 과제로 이분할 수 있으며, 향후 10년 간의 목표와 성과물을 제시하고 있다. FP7 ICT 연구프로그램 중 '미래 및 새로 부상하고 있는 기술(FET)' 프로젝트는 예상하지 못했던 아이디어와 발전에 효과적으로 대응하기 위하여 '개방형 FET프로젝트'와 '전 방위적 FET 프로젝트'의 두 가지 상호보완적 플랜을 운영하고 있다. 본 고에서는 이들 프로젝트에 대하여 성과목표, 기대효과, 자금지원 및 예산분배계획을 간략하게 소개하였다. 결론부에서는 앞에서의 논의를 토대로 우리나라의 미래 ICT 기술개발 전략 구상시 참조해야 할 정책적 시사점을 제시하였다.

• Biomolecules & Therapeutics
• /
• 제14권1호
• /
• pp.19-24
• /
• 2006

Atrial chambers serve as mechanosensory systems during the haemodynamic or mechanical disturbances, which initiates arrhythmia. Atrial myocytes, lacking t-tubules, have two functionally separate sarcoplasmic reticulums (SRs): those at the periphery close to the surface membrane, and those at the cell interior (center) not associated with the membrane. To explore possible role of fluid pressure (FP) in the regulation of atrial local $Ca^{2+}$ signaling we investigated the effect of FP on subcellular $Ca^{2+}$ signals in isolated rat atrial myocytes using confocal microscopy. FP was applied to whole area of single myocyte with pressurized automatic micro-jet (200-400 $mmH_2O$) positioned close to the cell. Application of FP enhanced spontaneous occurrences of peripheral and central $Ca^{2+}$ sparks with larger effects on the peripheral release sites. Unitary properties of single sparks were not altered by FP. Exposure to higher FP often triggered longitudinal $Ca^{2+}$ wave. These results suggest that fluid pressure may directly alter excitability of atrial myocytes by activating $Ca^{2+}$-dependent ionic conductance in the peripheral membrane and by enhancing spontaneous activation of central myofilaments.

• Journal of the Korean Society of Clothing and Textiles
• /
• 제25권2호
• /
• pp.339-349
• /
• 2001

This study was investigated the effects of ambient temperatures and menstrual cycle on Resting Metabolic Rate(RMR), Rectal Temperature(Tre), Skin(forehead, chest, abdomen, forearm, hand, thigh, leg, foot) Temperatures, and subjective thermal sensations in 8 young Korean females(ages 22-25, voluntarily). The Tre and the Skin Temperatures were measured in once every five minute for one hour. RMR was measured three times at 30 minutes intervals by indirect calorimetry. All measurements were gathered during Luteal Phase(LP), Menstruation(M), and Follicular Phase(FP) at two levels of ambient temperatures; low(17~21$^{\circ}C$) and middle(21.1~$25^{\circ}C$). LP were the highest values during FP and M in RMR, Tre, forehead temperature, chest temperature and abdomen temperature, while the leg(leg and foot) and arm(forearm and hand) temperatures were higher during FP rather than during LP at each ambient temperature. The downward curve of Tre in the experiment was larger during FP than LP. The values in subjective thermal sensations were most comfortable during LP than M and FP at each ambient temperature. The LP-FP differences in core and mean skin temperatures, and resting metabolic rate, were more significant at middle ambient temperatures than at low ambient temperatures.

• Journal of Korea Society of Digital Industry and Information Management
• /
• 제7권2호
• /
• pp.115-125
• /
• 2011

An accurate estimation of software development size is an important factor in calculating reasonable cost of project development and determining its success. In this study, we propose estimation models, using function point based on the functional correlation between software, with empirical data. Three models($FP_{est}(I)$, $FP_{est}(II)$, $FP_{est}(III)$) are developed with correlation and regression analysis. The validity of the models is evaluated by the significance test by comparing values of Mean Magnitude of Relative Error (MMRE) and predictions of each model at level n%. Model $FP_{est}(III)$ proved to be superior to other models such as IFPC(Indicative Function Point Count), EFPC(Estimated Function Point Count), EPFS(Early Prediction of Function Size), $FP_{est}(I)$, and $FP_{est}(II)$. As a result, the accuracy of the model appears to be very high to determine the usefulness of the model to finally overcome weakness of other estimation models. The model can be efficiently used to estimate project development size including software size or manpower allocation.

• Plant Resources
• /
• 제1권1호
• /
• pp.6-12
• /
• 1998

The Fp1 gene, originally isolated from red pepper seedlings, encode the iron storage protein, and have a high homology with ferritin genes at DNA and amino acid level. In order to determine ferritin protein expression in vegetative tissue. Fp1 gene was constructed in plant expression vector(PIG12IHm) and introduced in red pepper(var. Bukang, Chungyang and Kalag-Kimjang 2) via Agrobacterium tumefaciensmediated transformation. After selection on MS media containing Kanamycin(Km), putatively selected transformants were confirmed by amplification of selectable marker gene(Fp1 and NPII) by polymerase chain reaction. Northern blot showed that transcripts of Fp1 gene were detected in mature leaves of the plants. In A6, A7 and A8 and A14 of transgenic plants, transcript of Fp1 gene was increased seven-fold to eight-fold than other transgenic plants. Also the proteins obtained from leaves of transgenic plants were immunologically detected by Western blot using rabbit anti-ferritin polyclonal antibody. The expression protein appeared as strong band of apparent mass of 23.5kDa. suggesting the iron accumulation in transgenic red pepper plants.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 한국해양정보통신학회 2010년도 추계학술대회
• /
• pp.445-446
• /
• 2010

In Gen-2 Q-algorithm, the initial value of $Q_{fp}$, which is the slot-count parameter, is not defined in the standard. In this case, if we let the initial $Q_{fp}$ be large, the number of empty slot will be large during the initial query round. On the other hand, if the initial $Q_{fp}$ is small, almost all the slots will be collided. As a result, it is anticipated that the performance will be declined because the frame size does not converge to the optimal point quickly during the query round. In this paper, we analyze how the performances of Gen-2 Q-algorithm will be affected by the initial slot-count size.

• Journal of Animal Science and Technology
• /
• 제50권1호
• /
• pp.133-144
• /
• 2008

Four different pork patties were made with two levels, HP/FP-3% and HP/FP-6%, containing 3.0 and 6.0% HP(hot air dried sweet persimmon powder)/FP(freeze-dried sweet persimmon powder), respectively. After manufacture, the meat patties were packaged with  polyvinyl wrap and stored at 4℃ for 8 days. CTL(control) and HP-3% meat patties were significantly(p<0.05) higher in moisture content than the other samples. FP-6% was found higher in protein content than the other treatments. FP-3% had a higher fat content than other meat patty samples. However, ash contents were not found significantly(p>0.05) different among the meat patty samples. The value of pH, L* and a* values were decreased as the cold storage time increased in all treatments(p<0.05). WHC(water holding capacity) of CTL and HP-6% and cooking loss of HP-3% were significantly(p<0.05) decreased with increased storage period. The diameter and thickness of all meat patties decreased with increasing the storage period. VBN(volatile basic nitrogen) values of all meat patties were increased(p<0.05) with increased storage period. TBARS (thiobarbituric acid reactive substance) of treatments were higher than that of CTL during whole storage time. The number of microorganisms(Total plate counts, Escherichia coli.) were maintained below 4.61 log10 CFU/cm2 during the whole storage period. In sensory evaluation, treatment groups had higher(p<0.05) scores in aroma, flavor, color and overall acceptability.

• KIPS Transactions on Software and Data Engineering
• /
• 제5권3호
• /
• pp.155-164
• /
• 2016

For frequency pattern analysis of large databases, the new tree-based frequency pattern analysis algorithm which can compensate for the disadvantages of the Apriori method has been variously studied. In frequency pattern tree, the number of nodes is associated with memory allocation, but also affects memory resource consumption and processing speed of the growth. Therefore, reducing the number of nodes in the tree is very important in the frequency pattern mining. However, the absolute criteria which need to order the transaction items for construction frequency pattern tree has lowered the compression ratio of the tree nodes. But most of the frequency based tree construction methods adapted the absolute criteria. FP-tree is typically frequency pattern tree structure which is an extended prefix-tree structure for storing compressed frequent crucial information about frequent patterns. For construction the tree, all the frequent items in different transactions are sorted according to the absolute criteria, frequency descending order. CanTree also need to absolute criteria, canonical order, to construct the tree. In this paper, we proposed a novel frequency pattern tree construction method that does not use the absolute criteria, IRFP-tree algorithm. IRFP-tree(Intersection Rule based FP-tree). IRFP-tree is constituted with the new paradigm of the intersection rule without the use of the absolute criteria. It increased the compression ratio of the tree nodes, and reduced the tree construction time. Our method has the additional advantage that it provides incremental mining. The reported test result demonstrate the applicability and effectiveness of the proposed approach.

• The Journal of the Korea Contents Association
• /
• 제7권10호
• /
• pp.19-26
• /
• 2007

Since collaborative filtering has used the nearest-neighborhood method based on item preference it cannot only reflect exact contents but also has the problem of sparsity and scalability. The item-based collaborative filtering has been practically used improve these problems. However it still does not reflect attributes of the item. In this paper, we propose the method of associative group using the FP-Tree to solve the problem of existing recommendation system. The proposed makes frequent item and creates association rule by using FP-Tree without occurrence of candidate set. We made the efficient item group using $\alpha-cut$ according to the confidence of the association rule. To estimate the performance, the suggested method is compared with Gibbs Sampling, Expectation Maximization, and K-means in the MovieLens dataset.