• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.2
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• pp.78-84
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• 2006

A high performance liquid chromatography assay method for spiramycin in fish muscle was developed. The developed method was evaluated and validated by monitoring spiramycin in olive flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli) and in live conger eel (Anguilla japonica) in fish farms and distribution centers. Using the developed method, the recovery rate was up to 82.4-88.8%, which was higher than that of conventional methods (77.6-87.1%). In particular, the proposed sample treatment protocol was suitable for use with fish samples to remove low molecular weight materials and pigments that could interfere an accurate analysis. The prepared stock solution was very stable, and it remained chemically stable for 5 weeks at $4^{\circ}C$. The performance limit of the developed method for spiramycin acid in fish muscle was 0.05 ppm. One hundred thirty-four fish samples including olive flounder, black rock fish and live conger eel were analyzed to evaluate the overall efficiency of the modified method and to monitor the actual condition of spiramycin usage in fish farms. Olive flounder and black rock fish were collected from fish farms in coastal areas of Korea, and live conger eels were purchased from a fish market in the Busan area from September 2001 to March 2002. According to the overall performance of the developed method, it was considered suitable for the monitoring of spiramycin in fish muscle. The suggested method of analysis for spiramycin in fish muscle is registered in the Korean Official Methods of Food Analysis and is currently tieing utilized for fishery products inspection by the Korea Food and Drug Administration and the National Fisheries Products Quality Inspection Service.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.22 no.1
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• pp.11-18
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• 1986

Schooling behavier to a fishing gear and estimation of the volume of fish school in set net have ~ been studied by making use of such techniques as visual observations, underwater cameras, under- water televison. However, all of these observation techniques are subject to restrictions caused by illumination, underwater visibility, underwater transparent and sea conditions. For the above mentioned reasoa, one of the most effective method by this time become generally known a method using fish finder. In this paper, in order to control the fishing ground of set net effectively and to develope the telemetric fish finder, the experiments for the target strength, underwater shape of fishing gear, schooling behavier and volume of fish school with fish finder were performed at Galgott fishing ground of set net located Keouje Island, 15th-24th July and 18th-20th October in 1985. The results of these experiment showed that a method using fish finder in fishing grOlllld of set net is available for estimating distribution and school size, fish behavier in relation to a fishing gear and underwater shape of fishing gears.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.1
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• pp.80-89
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• 2021

In Korea, marine fish landed at ports are randomly sampled on a periodic basis (e.g., daily or weekly), and body sizes (e.g., lengths and weights) of those sampled fish are measured. The motivation for our study is whether or not such measurements reflect the size distribution, especially the length distribution of fish landed (= a population), because such length measurements are key data for a length-based assessment model. The current sampling method is to sample fish landed at ports by body size group (e.g., very small, small, medium, large, very large), using the sampling weights as the number of boxes by body size group. In this study, we showed that length composition data about fish sampled by the current method did not represent the length frequency distribution of the fish landed, and suggested that an alternative sampling method should be applied of using the sampling weights as the number of fish landed by body size group. We also introduced a method for determining an appropriate sample size.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2007.04a
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• pp.161-164
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• 2007

The paper studies in the wide-angle lens and distortion tendency and employs the correction techniques suitable to the fish-eye lens using the existing photographic survey methods. After carrying out the calibration of the the fish-eye lens, we calculated the correction parameters, and then developed the method that convert the original image-point to new image-point correcting distortion. The objectives of suggested calibration method in this paper are to calibrate the image of the the fish-eye lens used in the computer-vision and the control-instrumentation field widely. The proposed technique expects to improve the accuracy of the image of the fish-eye lens in the indoor tracking and monitoring field. Also the referenced cross point auto-extraction program is embodied for improving efficiency of the lens correction techniques. Consequently, this calibration method would be applied to the automated distorting correction method on not only the fish-eye lens also general lens.

• International Journal of Control, Automation, and Systems
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• v.1 no.1
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• pp.111-118
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• 2003

This paper proposes a two dimensional behavior analysis method for fish in a water tank based on the ARX method and the Kalman filter algorithm using image processing technology. In modeling the behavior of fish, the input is denoted as the environmental change and uses M-sequence. The output is expressed by the partnership between fish. The behavior model of individual fish is identified by the ARX method. It is then estimated by the Kalman filter algorithm. Finally, the fish behavior is analyzed by FFT. To prove the effectiveness of the pro-posed algorithm, it is applied to two tilapias in a water tank with dimensions of 100cm$\times$100cm$\times$50cm. The effectiveness of the proposed method is demonstrated through ARX identification, estimation of Kalman filter, and FFT analysis.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.508-513
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• 2008

Erythromycin has been used to treat Streptococosis, Edwardsiel1osis, Vibriosis, Bacterial enteritis in the cultured fish. In this study, a rapid and effective erythromycin analysis method with new sample treatment protocol and liquid chromatography/tandem mass spectrometry (LC/MS/MS) system for fish products was developed. For the erythromycin extraction from fish muscle, the solvent mixture composed of 0.2% meta-phosphoric acid and methanol (6:4) showed good recovery rate, and the optimum extraction solvent volume was 20 mL. Erythromycin detection using LC/MS/MS were carried out under electro spray ionization (ESI) positive condition and erythromycin mass value 576.2 and 157.9. And the detection limit of the established method was 0.005 mg/kg in fish products. The recovery rate of the developed method applied to the fish species were as following, olive flounder, $87.6{\pm}5.0%$; black rockfish, $87.2{\pm}6.4%$; eel, $85.2{\pm}4.8%$; and rainbow trout, $86.0{\pm}6.2%$. In the established method in this study, the correlation of coefficient values ($R^2$) of erythromycin calibration curve (n=11) was 0.9998.

• Korean journal of food and cookery science
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• v.26 no.4
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• pp.428-433
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• 2010

The aim of this research was to determine the relationship between quality of puffer fish and soak time in maturing water. This research used the cutting cranial nerve method, which is called the MSK method. The data was analyzed using the SPSS program. Based on salinity analysis result, both moisture content and pH were measured after 20 min of soaking. As the salinity of the maturing water increased, the moisture content initially decreased then increased once the salinity was greater than 3%. However, the concentration of the maturing water did not influence the pH level. The texture properties were measured to assess the effect of soak time in the maturing water. Hardness of the sample was highest (3.99) at 20 min, and cohesiveness also showed a maximum value (0.26) at 20 min. Gumminess and chewiness were highest 1.04 and 4.09, respectively, when the fish was matured for 20 min. Sensory properties were evaluated, and springiness, umami flavor, texture, and overall preference were highest at 20 min of soak time. The results showed that maturing the puffer fish for 20 min provided the best quality of texture and sensory characteristics for the fish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.6
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• pp.454-459
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• 2006

A simultaneous high-performance liquid chromatography assay method for amoxicillin and ampicillin in fish products was developed, evaluated, and validated by monitoring these antibiotics in fish samples obtained from aquaculture and distribution. The recovery rate of this method was higher than those of conventional methods and was 95.3-106.6% for amoxicillin and 81.4-92.4% for ampicillin. Our pretreatment procedure sufficiently removed or reduced materials affecting HPLC analysis, such as low-molecular-weight substances. The performance limit of this method was evaluated as 0.01 ppm of amoxicillin and ampicillin in fish muscle. Finally, 171 fish samples, including olive flounder (Paralichthys olivaceus), common sea bass (Lateolabrax japonicus), and black rock fish (Sebastes schlegeli) collected from fish farms in the coastal area between April and September 2005 were analyzed to evaluate the overall efficiency of the method and to monitor the actual of amoxicillin and ampicillin usage in fish farms. The results indicated that the developed method was suitable for analyzing amoxicillin and ampicillin in fish muscle, and determined that those antibiotics were being used for fish farming but were not detected in fish samples during the shipping and distribution stages.

• Journal of Korea Multimedia Society
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• v.12 no.1
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• pp.130-138
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• 2009

As time spent in front of the computer screens increases, an increasing number of people are using natural landscapes or virtual aquariums as their desktop and screen saver that can provide them with mental comfort. A virtual aquarium is constructed by an animation work that creates a variety of fish that freely move in a random virtual underwater environment to analyze their movement. This paper suggests a method that constructs an immersive virtual aquarium, using fluid animation method that expresses changes of shape of fluid in real time and the Smart Fish technology which is capable of an interaction according to the diverse characteristics of virtual fish. The suggested method can be used in a virtual aquarium, aquarium screen saver, virtual fish-raising game, etc., which express diverse undersea environment.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.60 no.2
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• pp.128-141
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• 2024

Fish resource surveys were conducted near Jeju Island in June, August and October 2021 using an underwater camera monitoring system, fish pots, and SCUBA diving methods. The efficiency of the methods used to survey fish resources was compared using the number of individuals compared to area per unit time (inds/m³/h) and the number of species compared to area per unit time (spp./m³/h). As a result of comparing the number of individuals compared to the area per unit time (inds/m³/h), the order was underwater camera 214.69, SCUBA diving 124.62, and fish pots 0.57 inds/m³/h. The number of species compared to area per unit time (spp./m³/h) is in the following order: SCUBA diving 0.85, underwater camera 0.38, and fish pots 0.01 spp./m³/h. The fish resource monitoring method using underwater cameras was found to be more efficient in individual counts, and the SCUBA diving method was found to be more efficient in species counts. When considering cost and survey efficiency, the fish resource survey method using underwater cameras was judged to be more effective. The results of this study are expected to be widely used in estimating the population density of fish, which is the core of future fisheries resource surveys.