• Structural Engineering and Mechanics
• /
• v.70 no.5
• /
• pp.551-562
• /
• 2019

In the present study, a suitable mathematical model considering parabolic transverse shear strains for dynamic analysis of laminated composite skew plates under different types of impulse and spatial loads was presented for the first time. The proposed mathematical model satisfies zero transverse shear strain at the top and bottom of the plate. On the basis of the cubic variation of thickness coordinate in in-plane displacement fields of the present mathematical model, a 2D finite element (FE) model was developed including skew transformations in the mathematical model. No shear correction factor is required in the present formulation and damping effect was also incorporated. This is the first FE implementation considering a cubic variation of thickness coordinate in in-plane displacement fields including skew transformations to solve the forced vibration problem of composite skew plates. The effect of transverse shear and rotary inertia was incorporated in the present model. The Newmark-${\beta}$ scheme was adapted to perform time integration from step to step. The $C^0$ FE formulation was implemented to overcome the problem of $C^1$ continuity associated with the cubic variation of thickness coordinate in in-plane displacement fields. The numerical studies showed that the present 2D FE model predicts the result close to the analytical results. Many new results varying different parameter such as skew angles, boundary conditions, etc. were presented.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.12
• /
• pp.1656-1666
• /
• 2021

Dental pathogens lead to chronic diseases like periodontitis, which causes loss of teeth. Here, we examined the plausible antibacterial efficacy of copper nanoparticles (CuNPs) synthesized using Cupressus macrocarpa extract (CME) against periodontitis-causing bacteria. The antimicrobial properties of CME-CuNPs were then assessed against oral microbes (M. luteus. B. subtilis, P. aerioginosa) that cause periodontal disease and were identified using morphological/ biochemical analysis, and 16S-rRNA techniques. The CME-CuNPs were characterized, and accordingly, the peak found at 577 nm using UV-Vis spectrometer showed the formation of stable CME-CuNPs. Also, the results revealed the formation of spherical and oblong monodispersed CME-CuNPs with sizes ranged from 11.3 to 22.4 nm. The FTIR analysis suggested that the CME contains reducing agents that consequently had a role in Cu reduction and CME-CuNP formation. Furthermore, the CME-CuNPs exhibited potent antimicrobial efficacy against different isolates which was superior to the reported values in literature. The antibacterial efficacy of CME-CuNPs on oral bacteria was compared to the synergistic solution of clindamycin with CME-CuNPs. The solution exhibited a superior capacity to prevent bacterial growth. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and fractional inhibitory concentration (FIC) of CME-CuNPs with clindamycin recorded against the selected periodontal disease-causing microorganisms were observed between the range of 2.6-3.6 ㎍/ml, 4-5 ㎍/ml and 0.312-0.5, respectively. Finally, the synergistic antimicrobial efficacy exhibited by CME-CuNPs with clindamycin against the tested strains could be useful for the future development of more effective treatments to control dental diseases.

• Microbiology and Biotechnology Letters
• /
• v.52 no.1
• /
• pp.24-36
• /
• 2024

This study determines the combinatorial effect of enterocin MSW5 and five essential oils (EOs- Thymus vulgaris, Cymbopogon martini, Origanum vulgare, Cinnamomum zeylanicum, and Cymbopogon citrus) against Staphylococcus aureus, Listeria monocytogenes, and Salmonella Typhimurium. The Minimum Inhibitory Concentration of each antimicrobial agent was determined. The MIC of enterocin MSW5 against test pathogens was in the following order: S. aureus (0.362 ± 0.01), S. Typhimurium (0.362 ± 0.05 mg/ml), L. monocytogenes (0.725 ± 0.08 mg/ml). Among all EOs, maximum activity was observed in the case of C. zeylanicum against S. aureus (78.12 ± 0.04 ppm), S. Typhimurium (78.12 ± 0.08 ppm), and L. monocytogenes (39.00 ± 0.05 ppm). Further, the checkerboard assay was used to determine the synergistic effect between antimicrobial agents and enterocin MSW5 in combination with C. zeylanicum has shown significant synergism with the Fraction Inhibitory Concentration index (0.372) against test pathogens. Additionally, individual EOs and enterocin MSW5 have shown anti-biofilm activity, whereas their combined use has shown more significant antibiofilm activity. The maximum anti-biofilm activity was observed with the combination of enterocin MSW5 and O. vulgares against S. aureus (92.86 ± 0.06%) and S. Typhimurium (73.63 ± 0.23%) and a combination of enterocin MSW5 and C. citrus against L. monocytogenes (87.84 ± 0.15%). Therefore, combinations of antimicrobial compounds can control the growth of foodborne pathogens better than the individual agent.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.23 no.2
• /
• pp.20-37
• /
• 2010

Purpose: The objective of this study was to observe the antibacterial effects of Sasangjasaebang (SSJSB) aqueous extracts, traditionally used for treating various gynecological diseases including vaginitis in Korea and their individual components - Cnidi Fructus(CF), Zanthoxyli Fructus(ZF) and Alumen(AL) against Gardnerella vaginalis ATCC14018, and combination effects of SSJSB extracts and Metronidazole were also monitored in this study. Methods: Antibacterial activities against Gardnerella vaginalis ATCC14018 of SSJSB (CF : ZF : AL = 10 : 3 : 3) aqueous extracts and their individual components were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were also monitored at MIC and $MIC{\times}2$ levels. The combination effects of SSJSB aqueous extracts and Metronidazole were observed by Checkboard Microtiter Assay and effects on bacterial growth curve treated with SSJSB aqueous extracts MIC + Metronidazole MIC, 1/2MIC and 1/4MIC, respectively. In the present study, Gardnerella vaginalis ATCC14018 were incubated under $37^{\circ}C$, 10% CO2 and bacterial growth curves were calculated at 24, 48, 72, 96 and 120 hrs after incubations. Results: MIC of CF aqueous extracts, ZF aqueous extracts, AL powders and SSJSB aqueous extracts against Gardnerella vaginalis ATCC14018 were detected as $27.5\;{\pm}\;13.693$(12.5~50), $6.875\;{\pm}\;3.423$(3.125~12.5), $4.375\;{\pm}\;1.713$(3.125~6.25) and $2.188\;{\pm}\;0.856(1.563{\sim}3.125)mg/m{\ell}$, respectively. MIC of Metronidazole was detected as $7.6\;{\pm}\;5.367(2{\sim}16){\mu}g/m{\ell}$ at same conditions. In addition, Metronidazole, CF aqueous extracts, ZF aqueous extracts, AL powders, and SSJSB aqueous extracts showed marked dosage-dependent inhibition of bacterial growth, and more dramatical inhibitions were detected in Metronidazole 1/2 MIC + SSJSB aqueous extracts MIC treatment as compared with each of single Metronidazole MIC and SSJSB aqueous extracts MIC treatments, respectively. Especially, quite similar inhibitory effects on bacterial growth were detected in Metronidazole 1/4 MIC + SSJSB aqueous extracts MIC treatment as compared with single Metronidazole MIC treatment in the present study. FIC index were detected as $0.475\;{\pm}\;0.137$(0.375~0.625) at Checkboard Microtiter Assay. Conclusion: The results obtained in this study suggest that CF aqueous extracts, ZF aqueous extracts, AL powders, and SSJSB aqueous extracts showed antibacterial effects against Gardnerella vaginalis ATCC14018, and they also showed dosage-dependent inhibitory effects on the bacterial growth. More potent antibacterial effects were detected in SSJSB aqueous extract as compared with individual components, respectively. In addition, combination treatment of SSJSB aqueous extract and Metronidazole showed more potent inhibitory effects on the growth of Gardnerella vaginalis with FIC index $0.475\;{\pm}\;0.137$(0.375~0.625). According to these results, the combination of SSJSB aqueous extract and Metronidazole is synergistic, and it is expected that effective dosages of Metronidazole could be reduced to 1/4 levels in combination with SSJSB extracts. And it might be needed to make further studies to seek the herbs which have antibacterial effects on the Gardnerella vaginalis ATCC14018.

• Korean Journal of Food Science and Technology
• /
• v.48 no.3
• /
• pp.241-246
• /
• 2016

The object of this study was to develop an alternative way to treat human skin pathogens using marine algae. During this study, we observed that the ethanolic extract of the edible brown algae [Sargassum serratifolium (C. Agardh) C. Agardh] exhibited potential antimicrobial activity against pathogenic commensal bacteria related with acne vulgaris (Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa), and Candida albicans which causes cutaneous candidiasis. Among the solvent-soluble fractions from the ethanolic extract, a hexane-soluble fraction showed the strongest antimicrobial activity against all tested human skin pathogens with MIC values ranging from 32 to $512{\mu}g/mL$. In addition, the hexane fraction exhibited a synergistic antimicrobial activity with commercial antibiotics used in the treatment of acne vulgaris or cutaneous candidiasis. Thus, this study suggests that S. serratifolium extract could be a potential source of natural antimicrobial agents or a pharmaceutical component against human skin pathogens.

• The Magazine of the IEIE
• /
• v.30 no.5
• /
• pp.95-95
• /
• 2003

현재의 라우팅 프로토콜은 다양한 사용자 요구를 만족시켜주기 위해서는 네트워크의 처리량을 최대화하고 동시에 사용자의 요구 시 QoS를 보장해주는 기법이 요구되고 있다. 기존의 최단경로 라우팅 프로토콜은 단일경로 라우팅으로 인해 병목현상의 단점을 지니고 있다. 즉, 원천과 목적지간 최단경로는 낮은 활용도를 나타내는 경로들이 많이 존재하지만 단일경로를 선택하므로서 폭주(congestion)의 발생확률이 높다. 최근에 들어 사용자의 QoS 요구 시, 다양한 QoS를 패킷 네트워크에서 처리할 수 있도록 IETF에서 DiffServ, RSVP, MPLS 등과 같은 패킷 QoS 기법에 대한 표준화 작업이 진행중이며, 그 중에서 Diffserv 네트워크가 대표적이다. 따라서 본 논문에서는 이 DiffServ 네트워크상에서 다양하게 유입되는 트래픽의 종류에 따라 사용자의 응용에 적절히 대응하여 트래픽을 처리하는 라우팅 기법 및 알고리즘을 연구하고 기존의 최선형 (Best effort) 트래픽을 처리하기 위한 트래픽 분산 라우팅 프로토콜 (Traffic-Balanced Rout-ing Protocol''TBRP)을 제안하였으며, 최적의 중간 노드를 선택하여 높은 순위의 상호형 데이터를 처리하기 위한 계층적 라우팅 프로토콜(또ierarchicalTra(fic-Scheduling Routing Protocol : HTSRP)을 연구하였다. 본 연구에서 제시한 프로토콜은 유, 무선망의 통합에 따른 다양한 엑세스망과 백본망에 유연한 트래픽 처리기법으로서 계층적 라우팅 알고리즘으로 적합하였다. 본 실험에서는 사용자의 QoS요청 시 제공되는 상호형 또는 스트리 밍 데이터를 위한 HTSRP_Q(Hierarchical Traffic-Scheduling Routing Pro-tocol for QoS)에 대해 성능이 우수함을 입증하였으며, 각 엑세스 단에서 요청하는 QoS 파라미터에 따라 자원을 최적화하여 QoS를 보장하고, 특히 지연에 민감한 트래픽을 처리하였으며, 제안한 프로토콜을 이용하여 사용자 요구 트래픽 종류에 따라 대화형 클래스, 스트리밍 클래스, 높은 순위의 상호형 클래스, 낮은 순위의 상호형 클래스, 그리고 background 클래스등 5개의 서비스 클래스로 분리하여 트래픽 특성에 맞게 처리할 수 있었다. QoS 관련 실험에서는 QoS 요청데이터를 균등하게 1에서 10Mbps 사이에 분포하였고 연결된 호에 대한 지속시간은 5분으로 하였다. 이러한 환경에서 프로토콜을 MaRS에 의해 실험을 하였고 기존의 거리-벡터 라우팅과 링크-상태 라우팅 프로토콜과 비교해서 처리량, 메시지 손실, 블럭킹율 등에서 비교적 우위의 성능을 확인할 수 있었으며, 특히, 차별화된 서비스의 특성에 맞게 라우팅 기법을 적용하므로서 망의 효율성과 안정성을 꾀할 수가 있었다. 연결 수 대 처리량에서는 HTSRP 프로토콜이 연결이 적을 때 DVR, LSR보다 우월하였으며 특히, 선형을 유지하였다. 연결 수 대 패킷 손실에서 HTSRP프로토콜에서 메시지 손실은 연결의 수가 낮거나 높을 때 다른 DVR과 LSR 라우팅 프로토콜과 유사한 결과를 나타내었다. Hotspo에서 TBRP, HTSRP프로토콜은 hotspot 연결의 수가 9일 때까지 DVR, LSR 보다 좋은 처리량를 나타냈고 HTSRP는 연결의 수가 6 이상일 때 가장 높은 처리량을 나타내었다. 일반 트래픽과 QoS 트래픽이 흔재할 경우는 트래픽이 증가할수록 HTSRP_Q가 가장 월등하였으며 , 로드가 증가할수록 낮은 블록킹률을 나타내었다. 본 논문에서는 점대점 전송을 기반으로 하였다. 앞으로 다양한 응용 S/W는 멀티캐스트 기반이 예상되므로 멀티캐스트 라우팅에 대한 연구가 필요하다. 본 논문의 프로토콜은 원천과 목적지간의 최단경로가 폭주상태가 아닌 해당 중간 노드를 이용한다. 최단경로의 모든 링크상의 트래픽 부하가 낮을 때 중간노드의 사용은 지연을 증가시킨다. 향후 최적의 성능을 위해 보완이 필요하다. 아울러, 2계위에서는 일반 트래픽과 QoS 트래픽이 혼재할 때 자동으로 네트워크의 효율적을 고려한 방법 선택이 필요하다.

• The Magazine of the IEIE
• /
• v.30 no.5
• /
• pp.551-551
• /
• 2003

현재의 라우팅 프로토콜은 다양한 사용자 요구를 만족시켜주기 위해서는 네트워크의 처리량을 최대화하고 동시에 사용자의 요구 시 QoS를 보장해주는 기법이 요구되고 있다. 기존의 최단경로 라우팅 프로토콜은 단일경로 라우팅으로 인해 병목현상의 단점을 지니고 있다. 즉, 원천과 목적지간 최단경로는 낮은 활용도를 나타내는 경로들이 많이 존재하지만 단일경로를 선택하므로서 폭주(congestion)의 발생확률이 높다. 최근에 들어 사용자의 QoS 요구 시, 다양한 QoS를 패킷 네트워크에서 처리할 수 있도록 IETF에서 DiffServ, RSVP, MPLS 등과 같은 패킷 QoS 기법에 대한 표준화 작업이 진행중이며, 그 중에서 Diffserv 네트워크가 대표적이다. 따라서 본 논문에서는 이 DiffServ 네트워크상에서 다양하게 유입되는 트래픽의 종류에 따라 사용자의 응용에 적절히 대응하여 트래픽을 처리하는 라우팅 기법 및 알고리즘을 연구하고 기존의 최선형 (Best effort) 트래픽을 처리하기 위한 트래픽 분산 라우팅 프로토콜 (Traffic-Balanced Rout-ing Protocol'TBRP)을 제안하였으며, 최적의 중간 노드를 선택하여 높은 순위의 상호형 데이터를 처리하기 위한 계층적 라우팅 프로토콜(또ierarchicalTra(fic-Scheduling Routing Protocol : HTSRP)을 연구하였다. 본 연구에서 제시한 프로토콜은 유, 무선망의 통합에 따른 다양한 엑세스망과 백본망에 유연한 트래픽 처리기법으로서 계층적 라우팅 알고리즘으로 적합하였다. 본 실험에서는 사용자의 QoS요청 시 제공되는 상호형 또는 스트리 밍 데이터를 위한 HTSRP_Q(Hierarchical Traffic-Scheduling Routing Pro-tocol for QoS)에 대해 성능이 우수함을 입증하였으며, 각 엑세스 단에서 요청하는 QoS 파라미터에 따라 자원을 최적화하여 QoS를 보장하고, 특히 지연에 민감한 트래픽을 처리하였으며, 제안한 프로토콜을 이용하여 사용자 요구 트래픽 종류에 따라 대화형 클래스, 스트리밍 클래스, 높은 순위의 상호형 클래스, 낮은 순위의 상호형 클래스, 그리고 background 클래스등 5개의 서비스 클래스로 분리하여 트래픽 특성에 맞게 처리할 수 있었다. QoS 관련 실험에서는 QoS 요청데이터를 균등하게 1에서 10Mbps 사이에 분포하였고 연결된 호에 대한 지속시간은 5분으로 하였다. 이러한 환경에서 프로토콜을 MaRS에 의해 실험을 하였고 기존의 거리-벡터 라우팅과 링크-상태 라우팅 프로토콜과 비교해서 처리량, 메시지 손실, 블럭킹율 등에서 비교적 우위의 성능을 확인할 수 있었으며, 특히, 차별화된 서비스의 특성에 맞게 라우팅 기법을 적용하므로서 망의 효율성과 안정성을 꾀할 수가 있었다. 연결 수 대 처리량에서는 HTSRP 프로토콜이 연결이 적을 때 DVR, LSR보다 우월하였으며 특히, 선형을 유지하였다. 연결 수 대 패킷 손실에서 HTSRP프로토콜에서 메시지 손실은 연결의 수가 낮거나 높을 때 다른 DVR과 LSR 라우팅 프로토콜과 유사한 결과를 나타내었다. Hotspo에서 TBRP, HTSRP프로토콜은 hotspot 연결의 수가 9일 때까지 DVR, LSR 보다 좋은 처리량를 나타냈고 HTSRP는 연결의 수가 6 이상일 때 가장 높은 처리량을 나타내었다. 일반 트래픽과 QoS 트래픽이 흔재할 경우는 트래픽이 증가할수록 HTSRP_Q가 가장 월등하였으며 , 로드가 증가할수록 낮은 블록킹률을 나타내었다. 본 논문에서는 점대점 전송을 기반으로 하였다. 앞으로 다양한 응용 S/W는 멀티캐스트 기반이 예상되므로 멀티캐스트 라우팅에 대한 연구가 필요하다. 본 논문의 프로토콜은 원천과 목적지간의 최단경로가 폭주상태가 아닌 해당 중간 노드를 이용한다. 최단경로의 모든 링크상의 트래픽 부하가 낮을 때 중간노드의 사용은 지연을 증가시킨다. 향후 최적의 성능을 위해 보완이 필요하다. 아울러, 2계위에서는 일반 트래픽과 QoS 트래픽이 혼재할 때 자동으로 네트워크의 효율적을 고려한 방법 선택이 필요하다.

• The Journal of the Korean Society for Microbiology
• /
• v.21 no.1
• /
• pp.1-16
• /
• 1986

Fifty-one strains of Pseudomonas aeruginosa were isolated from various clinical specimens. Among them, 26 (51%) strains were gentamicin-resistant (Gm') and 25 (49%) were susceptible to gentamicin (Gm'). The frequencies of resistant strains to piperacillin (Pi), cefotaxime, moxalactam, cefoperazone (Cz), and amikacin (Ak) ranged from 21.6 to 31.4%, and $MIC_{50}$ of these drugs were lower than the critical concentrations of susceptibility and resistance. Thirty (58.8%) strains were multiply resistant to 12 or more drugs. All Gm' strains were multiply resistant to 12 or more drugs and one was resistant to all 18 drugs tested, while only four Gm' strains were multiply resistant to 12 drugs and the multiplicity of resistance of the other Gm' strains were less than 10 drugs. Resistance to Gm appeared to have a significant correlation with the resistance to tobramycin (Tb), Ak, Pi, and Cz. All Gm' strains were resistant to Tb and about 38.4 to 46.1% of them were resistant to Ak, Pi, and Cz. The incorporation of $Ca^{++}$ and $Mg^{++}$ ions in Mueller-Hinton agar (MHA) did not influence the MICs of Gm, Tb, carbenicillin (Cb), Pi, and Cz as compared with the results obtained in MHA without these ions. Gm strains were studied on the combined effect of beta-lactam antibiotics and aminoglycosides by the methods of checkerboard and modified paper strip diffusion. Most Gm' strains showed significant synergistic effects by the FIC index between Ak and three beta-lactam antibiotics; Cb, Pi, and Cz, but these results did not in agreement the results obtained through the method of modified paper strip diffusion test. In order to know the nature of the drug resistance of P. aernginosa, the plasmid profile analysis was studied. Agarose gel electrophoresis of lysates processed by the method of Kado and Liu showed one or more plasmids in 22 (43.1%) strains. A group of 19 strains showed at least one band of plasmid and three strains two bands. The range of the molecular weight of plasmids was 3.8 to 243 Mdal. All strains carrying large plasmids larger than 200 Mdal were isolated from wound specimens. Three Gm' strains also harboured the plasm ids of 13 to 203 Mdal.

• KSBB Journal
• /
• v.26 no.5
• /
• pp.459-464
• /
• 2011

In this study, the canadian peat moss extract was purified by a supercritical ₂ using three different conditions and assessed its biological activities. Peat moss was extracted by acid-alkaline extraction method (sample 1) and purified by a supercritical $CO_2$ at $40^{\circ}C$ under pressure of 100 bar (sample 2), 120 bar (sample 3) or 150 bar (sample 4). We evaluated the antioxidant activities of the samples by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging, $Fe^{2+}$/ascorbate (FTC) and 2-thiobarbituric acid (TBA) methods. The antioxidant activities were examined by comparing the results with that of ascorbic acid as a positive control. Sample 3 showed relatively higher DPPH radical-scavenging activities than other samples. The antioxidant activity by FIC method exhibited similar results as the DPPH radical-scavenging activities. On the other hand, sample 2 showed higher antioxidant activity measured by TBA method of all. The whitening effects of the samples were examined using mushroom tyrosinase and B16F10 melanoma cells. Sample 3 exhibited overall significant whitening effects, however, other samples showed relatively lower effects. These results suggest that the peat moss extract purified by a supercritical ₂ could be used as a cosmetic ingredient for the anti-aging and whitening effects.

• Korean Journal of Veterinary Research
• /
• v.42 no.1
• /
• pp.45-54
• /
• 2002

The study was carried out to characterize the pharmacokinetics after intravenous (iv, 20 mg/kg) and oral (p.o. 100 mg/kg) administration as oxytetracycline (OTC) and tiamulin (TIA) mixture in swine and to determine interaction between OTC and TIA against various pig pathogenic bacteria. The antibacterial effects of OTC in combination with TIA in vitro showed synergistic effect against Salmonella typhimurium 1925, Pasteurella multocida Type A, P. multocida Type D, Krebsiella Pneumoniae 2001, K. Pneumoniae 1560, K. Pneumoniae 2208, Haemophillus pleuropneumonia S 2, and H. pleuropneumonia S 5, but against additive effect E. coli K88ab and S. choleraesuis on the basis of fractional inhibitory concentration (FIC) index. On the while, after i.v. and p.o. administration of OTC and TIA mixture, each OTC and TIA concentrations in plasma were fitted to an open two-compartment model. After i.v. administration of OTC-TIA mixture, the mean distribution half-life ($T_{1/2{\alpha}}$) of OTC and TIA in plasma showed 0.29 h and 0.17 h, and the mean elimination half-life ($T_{1/2{\beta}}$) of those was 4.36 h and 6.64 h, respectively. The mean volume of distribution at steady state ($Vd_{ss}$) of OTC and TIA was $0.85{\ell}/kg$ and $2.44{\ell}/kg$, respectively. After oral administration of OTC and TIA mixture, the mean maximal absorption concentrations ($C_{max}$) of OTC and TIA were $0.60{\mu}g/m{\ell}$ at 1.07 h ($T_{max}$) and $1.68{\mu}g/m{\ell}$ at 1.85 h ($T_{max}$), respectively. The mean elimination half-life ($T_{1/2{\beta}}$) of those showed 6.84 h and 6.36 h. In conclusion, we could suggest in this study that the combination of OTC and TIA may be recommended for the antibacterial therapy against polymicrobial infections, and both OTC and TIA showed large distribution to tissues and high $C_{max}$ after p.o. administration.