• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.181-182
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• 2003

Exposure to some synthetic environmental chemicals and their metabolites cause reproductive problems in a variety of vertebrate via endocrine mechanisms. However, in most cases, the link between these compounds and adverse effects on humans, fish, and wildlife has not been established, which necessitates a closer look at the molecular, functional, and clinical implications of these chemicals in the environment. Calbindin-D9k (CaBP-9k) is a member of a largo family of intracellular calcium binding proteins that have high affinities for calcium. It was reported that the estrogen level of uterus affected the expression of the CaBP-9k gene in rat uterus. We examined the dose-dependent CaBP-9K gene expression in the uterus for three-days injection of methoxychlor (HC) in the overectomized immature rats and the relation with uterotrophic response of the compoundsand compared the responses induced by MC according to the route of administration.

• 한국수정란이식학회지
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• 제10권3호
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• pp.183-191
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• 1995

This experiment was carried out to study the determination of survival of vitrified and thawed mammal follicular oocytes by FDA-test. Oocytes were divided into 3 groups according to attachment of cumulus cell. Group A oocytes were tightly surrounded by cumulus cell, group B oocytes were partially surrounded by cumulus cell, and group C oocytes were poorly surrounded by cumulus cell. Vitrification solution developed by our previous study (Kim et al, 1992) which consisted of permeable agent (20 % glycerol + 10 % ethylene glycol) and nonpermeable agent (30 % Ficoll + 10 % sucrose). Oocytes (7~10) loaded into 0.25 ml straw after 10 min equilibration were plunged into liquid nitrogen (- 196$^{\circ}C$) directly. The FDA-score of vitrified and thawed group A oocytes was higher in rat (4.2) than in rabbit (3.9), cow (3.8), mouse (3.4) and porcine (2.4), however that of cumulus cell was higher in rabbit (4.7) than in rat (4.1), cow (2.9), porcine (2.6) and mouse (1.4). The FDA-score of vitrified and thawed group B oocytes were 3.1 (cow), 2.9 (rabbit), 2.9 (mouse), 2.6 (rat) and 2.5 (porcine), respectively. However that of cumulus cell was higher in rabbit (3.7) than in porcine (2.6), rat (2.3), cow (1.7) and mouse (0.3). The FDA-score of vitrified and thawed group C oocytes was higher in mouse (4.1) than in cow (2.9), rabbit (2.6), rat (1.3) and porcine (1.1). As shown in the above results, The survival rates of oocytes were higher in group A than in group B and C except in mouse and cow. These results suggest that the survival of cumulus cell as well as follicular oocytes can be reliably judged by their fluorescence with FDA-test.

• 한국농공학회논문집
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• 제51권5호
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• pp.101-106
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• 2009

The previous study developed the Filter-Disinfection-Adsorption (FDA) system to provide clean irrigation water for greenhouse cultivation as well as convenience water to farmers. In this study, the field examination was undertaken to assess performance of the FDA system. The field application was made in the suburb of Daegu, one of the large city in Korea. The study area located near by down-stream of Gum-Ho river is suffering low irrigation water quality problems with no water supply service facilities. Four water quality parameters including Suspended Solid (SS), Biological Oxygen Demand (BOD), coliform, and turbidity were selected to test the purification performance of FDA system. Also in order to improve the system, this study investigated the defects of using the FDA system through field monitoring. As results, it was found that this system can be used to supply good quality of irrigation water for greenhouse cultivation and also provide convenience water to farmers in the field areas of no water supply services.

• 한국농공학회논문집
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• 제51권5호
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• pp.95-100
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• 2009

The water purification systems have been hardly used for agricultural purpose due to their complicated compositions and high costs for farmers, while only simple filtrations have been applied to irrigation systems in order to prevent the system from clogging problems. This study therefore developed a clean water supplying system, the Filter-Disinfection-Adsorption (FDA) system, especially for greenhouse cultivation of where low quality of water is available. This system has also been produced for providing convenience water to farmers in the areas of no water supply service systems for the purpose of washing their bodies or agricultural machineries after farm work. The FDA system consists of three stages of purification processes with an integral module, including disk and teflon filtrations and Ultraviolet (UV) sterilization processes. Indoor experiments were undertaken with a trial product of the FDA system to test its performance. The operation test of the process was performed as well as the condition check of each item including UV module, filters, control panel, pump, valves, etc. The results shows good performance of each test with no critical problems. The initial and maintenance costs were also analysed with other purification systems. From the comparisons, the FDA system found to be very economical and easy to use.

• 한국해양학회지:바다
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• 제19권4호
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• pp.223-231
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• 2014

선박평형수를 통한 외래종 확산을 방지하기 위해 국제해사기구(IMO)에서는 2004년 선박평형수관리협약을 채택하였다. 이 협약에 따르면 앞으로 대부분의 선박들은 선박평형수 처리시스템을 통해서 해양 생물을 사멸 또는 제거시킨 후 배출해야 하며, 이는 플랑크톤의 생사판별방법을 통하여 이루어진다. 본 연구에서는 국제적으로 선박평형수 처리후 생사판별법으로 널리 사용되고 있는 fluorescein diacetate assay (FDA) 염색방법의 제한성과 이의 대안으로 Neutral red (NR) 염색방법사용 가능성을 살펴보고자 하였다. FDA 염색법은 대부분의 플랑크톤 염색에 되어서 현재 가장 널리 사용되는 방법임에도 불구하고 본 연구에서는 Ditylum brightwellii 을 제외한 모든 식물플랑크톤 대해서 낮은 염색 효율(전체 평균 염색 효율 <50%)을 보였으며, 식물플랑크톤이 갖는 고유한 형광(적색)에 간섭을 많이 받는 것으로 나타났다. 또한 FDA는 형광파장에 노출된 후 빠르게 색이 바래지는 경향도 관찰되었다. 반면에 NR은 조사된 모든 동 식물플랑크톤 개체수에 대해서 90% 이상의 높은 염색 효율을 보였다. 두 염색법 모두 포르말린을 이용해서 사멸시킨 동 식물플랑크톤에 대해서는 염색이 되지 않았다. 본 연구결과를 통해서 NR 염색법을 이용한 동 식물플랑크톤 생사판별은 매우 효율적이라고 판단된다.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.67-67
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• 2003

Tributyltin chloride (TBTCI) is an organotin compounds that have been widely used as antifouling agents and bioaccumulated in the food chain. TBTCI has been known to induce imposex in female gastropods. There are several reports that TBTCI increased testosterone level and inhibited the conversion of testosterone to estradiol by the aromatase cytochrome P450 enzyme. In this studies, we investigated the effects of TBTCI on steroidogenesis in testes, We dosed to 4-week-old Spragus-Dawleys (SD) male rats with TSTCI (0, 1, 5, 10, and 20mg/kg/day) daily by gavage for 14 days. TBTCI significantly decreased the weights of seminal vesicle, prostate, cowper's gland and LABC at 10 and 20mg/kg/day but significantly Increased the weights of liver at 10 and 20mg/kg/day and adrenals at 20mg/kg/day. mRNA levels of steroidogenic acute regulatory (StAR) and P450 aromatase were decreased and mRNA levels of cytochrome P450 17$\alpha$-hydroxylase/$C_{17-20}$ lyase (P450c17) were increased by TBTCI. TBTCI significantly increased serum testosterone level in dose-dependent manner. From above results, we found that TBTCI altered mRNA levels of enzymes related steroidogenesis, weights of organs and serum testosterone levels. This suggests that change of hormone levels may be due to alteration of mRNA levels of steroidogenic enzyme in testes, but further studies are necessary to investigate hormone levels in testis organ in order to find a relation of enzyme related to steroidogenesis with hormone levels. This work was supported by the Korea FDA Grant KFDA-03131-EDS-010.

• Ocean and Polar Research
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• 제31권4호
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• pp.349-357
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• 2009

Ballast water is widely recognized as a serious environmental problem due to the risk of introducing non-indigenous aquatic species. In this study we aimed to investigate measures which can minimize the transfer of aquatic organisms from ballast water. Securing more reliable technologies to determine the viability of aquatic organisms is an important initiative in ballast water management systems. To evaluate the viability of marine phytoplankton, we designed the staining methods of fluorescein diacetate (FDA) and Calcein-AM assay on each target species belonging to different groups, such as bacillariphyceae, dinophyceae, raphidophyceae, chrysophyceae, haptophyceae and chlorophyceae. The FDA method, which is based on measurements of cell esterase activity using a fluorimetric stain, was the best dye for determining live cells of almost all phytoplankton species, except several diatoms tested in this study. On the other hand, although fluorescence of Calcein-AM was very clear for a comparatively longer time, green fluorescence per cell volume was lacking in most of the tested species. According to the Flow CAM method, which is a continuous imaging technique designed to characterize particles, green fluorescence values of stained cells by FDA were significantly higher than those of Calcein-AM treatments and control, implying that the Flow CAM using FDA assay could be adapted as an important tool for distinguishing living cells from dead cells. Our results suggest that the FDA and Calcein-AM methods can be adapted for use on phytoplankton, though species-specific characters are greatly different from one organism to another.

• 한국가축번식학회지
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• 제13권3호
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• pp.134-140
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• 1989

Studies were conducted to seek reasonable methods of rapid freezing of mouse embryos using liquid nitrogen. The effects of the cryoprotectants concentration and the substitution of raffinose to sucrose in freezing and dilution medium on mouse embryo survival rates were determined using the FDA-test. The summarized results are as follows : 1. When 0.3M of sucrose was added into the freezing and dilution medium, FDA scores of embryos were 1.48(1.5M), 3.81(3.0M) and 4.10(4.5M). Higher FDA scores of embryos were obtained in 3.0M and 4.5M glycerol concentrations (P<0.05). 2. With the addition of 0.3M raffinose to the freezing and dilution medium, FDA scores of embryos did not significantly differ between glycerol levels ; 3.97(1.5M), 4.11(3.0M) and 3.54(4.5M). Higher scores of embryos existed in 3.0M glycerol concentration. 3. Concentration of sucrose or raffinose in freezing and dilution medium affected FDA scores of embryos. When sucrose concerations of 0.3, 0.5 and 1.0M were added to the freezing medium, FDA scores of embryos were 3.12, 2.38 and 0, respectively. However, when the same concentrations of raffinose were added to freezing medium, the FDA scores were 4.21, 2.91 and 0. In both cases, better FDA scores of embryos were attained in 0.3M of sucrose or raffinose (P<0.01).

• 한국수정란이식학회지
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• 제11권1호
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• pp.41-50
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• 1996

This experiment was carried out to investigate the ovarian responses of the ovulation point, ovarian weight and size, the number of ovarian follicles and collected embryos, and to study the effects of the developmental stages (oocytes, 2-4 cell. 8-16 cell and morulae), additional levels of Ficoll (0, 15, 30%) on the survival rate (FDA-test) of rat embryos frozen in vitrification solution (20% glycerol + 10% ethylene glycol + 10% sucrose). Sunanarized results was as follows; 1. The mean ovulation point per head was 7, and the weight of ovaries was 0.03g. The size of ovary was 5.9 mm(L) and 4.6 mm(W), and the number of ovarian follicles over and below 2 mm was 4.7 and 8.7, respectively. The number of the collected embryos per head was 5.5 (79%). 2. 2. The FDA score of embryos frozen in 20 G 10 E 10 S without Ficoll was 2.8 (oocyte), 2.6 (2-4 cell), 3.9 (8-16 cell) and 3.6 (morula), respectively. However, there were no significant differences among treatments. 3. The FDA score of embryos frozen in 20 G 10 E 10 S with 15% Ficoll was 3.4 (oocyte), 4.0 (2-4 cell), 4.7 (8-16 cell) and 4.8 (morulae), respectively (P>0.05). 4. The FDA score of embryos frozen in 20 G 10 E 10 S with 30 % Ficoll was 3.7 (oocyte), 3.2 (2-4 cell), 4.4 (8-16 cell) and 4.4 (morulae), respectively (P>0.05). 5. As shown in the above results, the higher survival rate was obtained in the treatment of 15% Ficoll than that of 30%. And the survival rate (FDA-test)of the oocytes and 2-4 cell stages of the rat embryos was lower than that of 8~16 cell and morulae stages. It was considered that 8-16 cell and morulae could be available for the successful freezing by vitrification of rat embryos with 15% Ficoll except for oocytes.

• 한국항행학회논문지
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• 제13권1호
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• pp.74-78
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• 2009

본 논문은 IP-USN 기반의 FDA(Fault Detection Agent)의 설계와 구축을 설명한다. FDA는 IP-USN 기반의 홈 네트워크 환경에서 멀티미디어 원격 교육을 위한 소프트웨어 오류를 감지하기에 적합한 에이전트이다. 이 시스템은 ED, ES로 구성되어 있다. ED는 IP-USN 기반 환경에서 멀티미디어 원격 교육을 위하여 훅킹 기법으로 오류를 감지하는 에이전트이다. ES는 IP-USN 기반 환경에서 멀티미디어 원격 교육을 위하여 오류를 공유하는 에이전트이다.