This study was to evaluate the efficacy of single dose Mycoplasma hyopneumoniae (M. hyo)-vaccination in the swine farms which had different serological patterns of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). A minimum of 240 pigs from each farm was applied, allocating M. hyo vaccinated and control groups. The PRRSV and PCV2 infections were analyzed by serological method (commercial ELISA kit). After administrating pigs a single dose of M. hyo vaccine or control saline at 3 weeks of age, serum antibodies to M. hyo, PRRSV and PCV2 were monitored at 4, 10, 16 and 22 weeks of age. Mortality, weight changes, feed conversion ratio (FCR) and lung score were also evaluated. A single-dose vaccination of M. hyo bacterin was efficacious to reduce mycoplasmal lung lesions and induce good humoral immune response. However, FCR was improved only in one of the three farms where showed seronegative status to both PRRSV and PCV2 in the period from 4 to 16 weeks of age. These results might imply that M. hyo vaccine alone could not overcome the PRRSV and PCV2 infection-associated wasting in the field condition. Therefore, the control of PRRSV and PCV2 should be considered to obtain the better effects of M. hyo vaccination.
The purpose of this study was to find out the effect that muscle activity of upper extremity and functional test in the case of the stroke patient by using the feedback mirror therapy. Sixteen subjects were recruited and randomly divided into two groups. one group was trained feedback mirror therapy and other group was action observation training. This process was carried out five times a week for eight weeks. To upper extremity test was used to MFT, FMA and in order to test UT, DM, BB, FCR, ECRL used to muscle activity. After the training, exclude FCR an upper extremity motor function of target showed significant difference between two groups and especially an experimental group showed significant muscle activity and MFT, FMA score improvement of UT, DM, BB, ECRL. Therefore, feedback mirror therapy is more upper extremity motor function and muscle activity improvement effect than action observation training.
The present experiment was conducted to assess the efficacy of methionine hydroxy analogue free fatty acid (MHAFA) in comparison to DL-methionine (DL-Met) utilizing day-old commercial broiler chicks (n=300). The chicks were randomly distributed into 30 groups of 10 chicks each. Three dietary treatments, viz. D1-maize-soybean meal based basal diet (Control), D2- control diet supplemented with DL- methionine to meet its requirements and D3-control supplemented with MHA-FA @ 1.54 times of DL-methionine used in D2, were formulated. Each dietary treatment was offered to 10 replicated groups of chicks following completely random design (CRD). The chicks fed on supplemental DL-Met had significantly higher (p<0.01) gain in body weight, followed by MHA-FA group and control during 0-3 weeks of age. During overall growth period (0-6 weeks), chicks in DL-Met and MHA-FA groups grew better (p<0.01) than those in control. Feed conversion ratio (FCR) improved (p<0.01) on supplementation of either DL- Met or MHA-FA in the basal (Control) diet during 0-3 weeks of age. The FCR for overall period, however, did not differ statistically (p>0.05) amongst the treatments. The eviscerated yields emanated from diets with DL-Met or MHA-FA were higher (p<0.01) than that in Control. Abdominal fat pad was also more (p<0.01) in broilers fed control diet than in DL-Met or MHA-FA supplemented group. Breast yield was higher (p<0.05) in MHA-FA fed broilers than those fed DL-methionine supplemented or un-supplemented diets. The efficacy of MHA-FA in comparison to DL-Met for growth was 62.11, 64.82 and 63.88% and for feed efficiency was 62.98, 67.73 and 64.01% at 0-3, 3-6 and 0-6 weeks of age, respectively, while it was 65.85, 71.40 and 67.49% for eviscerated yield, abdominal fat pad reduction and breast yield at 6 weeks of age, respectively.
In this study, we crossbred Epinephelus akaara and E. lanceolatus to produce a hybrid grouper with faster growth and adaptation to domestic aquaculture environments. The plasma cortisol and glucose levels and osmoregulation (stress response indicators) of the hybrid grouper, E. akaara ♀ × E. lanceolatus ♂, were investigated under several salinity levels (32, 24, 16, and 8 psu). The body lengths and weights of E. akaara (8.2 ± 0.1 cm, 8.3 ± 0.4 g) and the hybrid (8.6 ± 0.1 cm, 10.0 ± 0.4 g) were similar at the start of the experiment, but were significantly different at the end of the experiment. Juveniles of both E. akaara and the hybrid showed greater weight gain, specific growth, and feed conversion rate (FCR) under low salinity of 16 psu. Under the 8 psu treatment, the juvenile E. akaara all died, while the hybrid juveniles survived. Plasma cortisol levels were not affected by lower salinity in both species. The above results indicate that the hybrid is more tolerant of low salinity than is E. akaara, although both species exhibited higher growth and FCR at 16 psu, lower than the salinity of natural seawater. Thus, juveniles of both E. akaara and the hybrid can be more effectively cultured in brackish areas or waters with salinity lower than that of seawater.
A trial was conducted to evaluate the growth performance and carcass characteristics of LW ${\times}$ Mukota $F_1$ crosses when fed diets containing graded levels of maize cob meal. Sixteen LW ${\times}$ Mukota $F_1$ crossbred pigs of approximately 4.5 months of age, were randomly allocated to four diets that contained 0, 100, 200 and 300 g maize cobs/kg, which corresponded to 276.4, 360.3, 402.9 and 523.5 g NDF/kg, respectively. The pigs were fed ad libitum for 14 weeks. The diets were formulated to contain similar levels of energy (ca. 9MJ ME/kg) and protein (ca.160 g CP/kg). Average daily feed intake (ADFI), daily gain (ADG) and feed conversion ratio (FCR) were monitored for 14 weeks. At slaughter, the cold dressed weight (CDM) and backfat thickness (BFT) were determined. There was no significant linear relationship (p>0.05) between level of cob inclusion and ADFI. The ADG decreased as level of maize cobs increased (p<0.001). The pigs that were on 300 g cobs/kg had the highest FCR (p<0.05) as compared to pigs on the other three diets. No differences (p>0.05) were observed in the CDM between pigs that were fed diets that had 0 and 100 g maize cobs/kg. In addition, pigs on 100 and 200 g cobs/kg diets had the same CDM (p>0.05). The diet that contained 300 g cobs/kg gave the lowest (p< 0.001) CDM. Both BFT parameters showed a decrease (p<0.05) as level of maize cobs increased. An increase in CDM was associated with an increase in BFT (p<0.001), with the correlation coefficient between K7.5 and CDM being 0.84 (p<0.001). It can, therefore, be concluded that crossbred pigs perform well on diets containing up to 200 g/kg maize cobs.
The objective of these experiments gas to investigate the feeding of Powerfeel supplementation on performance and intestinal microflora of broiler chicks. Five treatments were allocated into 0, 0.025, 0.050, 0.075, 0.10% Powerfeel with four replications for five weeks. Diets contained 21.5% CP, 3,100 kcal/kg ME for the first three weeks and 19% CP, 3,100 kcal/kg ME for the rest two weeks. Weight gain, feed intake, feed conversion were weekly measured. E. coli and Salmonella were examined from cecum and ileum at the end of experiment. ND titer were also measured at five weeks old after first and booster vaccination at 14 and 21 days of age. In experiment 1, Weight gain of Powerfeef treatments was significantly higher for five weeks (P〈0.05). Feed intake increased on all Powerfeel treatments. Chicks fed Powerfeel improved feed conversion ratio(FCR) significantly compared to that of control (P〈0.05), but was not shown the consistency. In experiment 2, Weight gain and feed consumption of chicks fed Powerfeel were tended to increase, but were not statistically different from control. Powerfeel treatments showed significantly lower FCR than control(P〈0.05). The number of Salmonella and E. coli of chicks ileum fed Powerfeel were highly decreased, compared to those of control in both experiments. Powerfeel supplementation seemed to decrease the number of both Salmonella and E. coli in illeum, but was not consistency in cecum. Chicks fed Powerfeel showed significantly higher ND titer than control (P〈0.05). These results indicated that the optimum level of Powerfeel to maximize the performance of broiler chicks were estimated from 0.050 to 0.075% in broiler diets.
This study was conducted to determine the effect of Saccharomyces cerevisiae (SC) as a dietary probiotic and evaluated the most suitable feeding interval for this probiotic on growth performance, carcass yield, and meat quality parameters in broiler chickens. In total, 1,050 one-day-old Cobb 500 chicks were randomly assigned to one of seven dietary treatment groups, in a $2{\times}3$ factorial arrangement with 3 SC dosages (0.6%, 1%, and 1.4%) and two feeding intervals [long term (LT) for 35 days and short term (ST) for 28 days after hatching], with a negative control diet (NC; 0% SC). Triplicate experiments were performed with 50 birds per cage. Broilers fed a diet including SC showed increased (p<0.01) daily gain and feed efficiency compared to the control. Further, broilers fed the 1.4% SC supplemented diet showed a significantly increased (p<0.01) average daily gain (ADG) and feed conversion ratio (FCR) compared to broilers fed the 0.6% and 1% SC incorporated diets. Similarly, broilers fed an LT SC diet showed a greater (p<0.01) increase in ADG and FCR compared to broilers fed an ST SC diet. Moreover, broilers fed an LT SC diet displayed a reduced (p<0.05) meat pH, gizzard weight, and increased (p<0.05) meat water-holding capacity compared to broilers fed an ST SC diet. Broilers fed the 1.4% SC supplemented diet showed increased (p<0.05) thigh muscle weight compared to broilers fed the 0.6% and 1% SC supplemented diets. In conclusion, broilers fed LT SC diets showed improved growth performance and carcass quality parameters compared to broilers fed ST SC diets, and the NC diet, from hatching to day 35.
The objective of this experiment was to investigate the effect of Newcastle disease virus (NDV) and infectious bursal disease virus (IBDV) vaccination on performance of broiler chicks for five weeks. Two types of poultry houses and three patterns of vaccination ($NDV^-/IBDV^-$, $NDV^+/IBDV^-$ and $NDV^+/IBDV^+$) were factorially assigned to six treatments. NDV, B1 strain and IBDV, Bursin-2 vaccine were orally administered at 5, 14 and 7, 18 days, respectively. Forty eight hundred chicks were grouped into four replications with two hundnyd hybro $\times$ hybro chicks per each treatment. Weight gain, feed conversion ratio (FCR), mortality and product index were surveyed at the end of experiment. Bursa index and IBDV antibody titer of chicks were weekly measured. Weight gain of chicks vaccinated with $NDV^+/IBDV^+$ was significantly increased compared to that of other treatments at both window and windowless poultry houses (p<0.05). Chicks vaccinated with $NDV^+/IBDV^+$ also showed significantly improving the FCR and mortality compared to those of other treatments at both poultry houses (p<0.05). The bursa indecies of both poultry houses were high from one-day- to three-weeks-old, but were low for the rest of two weeks. IBDV antibody of all chicks was detected 100% by agar gel precipitation (AGP) test at one day old, but was not detected in $NDV^-/IBDV^-$ and $NDV^+/IBDV^-$ treatments at four weeks old. However, it showed 100% in $NDV^+/IBDV^+$ treatment. Antibody titer using ELISA showed similar trend to that of AGP test. The results of this experiment confirmed that IBDV and NDV combined vaccine significantly improved the performance of broiler chicks.
Craig, Aimee-Louise;Muns, Ramon;Gordon, Alan;Magowan, Elizabeth
Asian-Australasian Journal of Animal Sciences
/
v.33
no.8
/
pp.1301-1309
/
2020
Objective: To evaluate the use of nurse sows and post-weaning nutrition strategies for low wean weight (WW) pigs on lifetime growth and efficiency. Methods: Animals (n = 270) were assigned to one of five treatments at 28 d. Low WW pigs (<6 kg) were either weaned and offered a special dietary regime recommended for low WW pigs (WEAN) or placed on a nurse sow (NURSE) and weaned at 49 d. Normal WW pigs (9 kg) (NORM) were also weaned at 28 d. After weaning, NORM and NURSE pigs were offered either a 'high' (4 kg/pig of starter 1 diet followed by 8 kg/pig of starter 2 diet) or 'low' (8 kg/pig of starter 2 diet) starter diet allowance in a 2×2 factorial arrangement. A typical grower diet was then offered, followed by a typical finisher diet until 147 d of age. Results: NORM pigs where heavier throughout their life compared to NURSE pigs (91.4 kg vs 76.2 kg at 147 d; p<0.001). WEAN pigs were heavier at 70 d compared to NURSE pigs (23.9 kg vs 21.0 kg; p<0.001), but there was no significant difference at 147 d between NURSE and WEAN treatments. NURSE pigs had reduced feed intake throughout the finishing period (1.6 kg/d; p<0.001) compared to WEAN (2.0 kg/d) and NORM (1.9 kg/d) pigs. Feed conversion ratio (FCR) of NURSE (2.20) was lower than NORM and WEAN during the finishing period (2.40 and 2.79, respectively). Conclusion: Extended (up to 49 d) nursing for low WW pigs resulted in improved FCR during the finishing period, but no overall improvement in growth rate compared to low WW pigs weaned at 28 d and offered a specialised starter regime. Normal WW pigs where significantly heavier than low WW pigs throughout the study.
This study evaluated the effects of glycerol polyethylene glycol ricinoleate (GPGR) supplementation in different energy density diets on the growth performance, blood profiles, excreta gas emission, and total tract apparent retention (TTAR) of nutrients in broilers. A total of 544 one-day-old male Ross broilers were used in a 35-day trial. The broilers were allocated into one of four treatment groups in a $2{\times}2$ factorial arrangement with two levels of energy densities (a normal energy or decreased energy density) and GPGR (0 and 0.035%). From day 18 to 35, the GPGR supplemented and normal energy density diet groups showed a significantly improved (p < 0.05) body weight gain (BWG). Meanwhile, the GPGR supplemented diet group had a significantly reduced (p < 0.05) feed conversion ratio (FCR) compared to that of the non-supplemented diet group. From day 0 to 35, the GPGR supplemented diet and the normal energy density diet groups had a significantly increased (p < 0.01) BWG and a reduced (p < 0.01) FCR. Moreover, GPGR supplementation tended to increase (p < 0.1) the TTAR of the dry matter (DM) compared with the non-supplemented diets. Likewise, the normal energy density diets had a significantly improved TTAR for the gross energy (GE) (p < 0.05) than that of the decreased energy density diets. No interactive effects were observed between the energy density and GPGR supplemented diets. In conclusion, both dietary GPGR supplementation and normal energy density diets had beneficial effects on the growth performance of broiler chickens without any adverse effects on blood profiles and excreta gas emission.
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