• Biomolecules & Therapeutics
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• v.20 no.1
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• pp.43-49
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• 2012

Stimulation of mast cells through the high affinity IgE receptor (Fc${\varepsilon}$RI) induces degranulation, lipid mediator release, and cytokine secretion leading to allergic reactions. Although various signaling pathways have been characterized to be involved in the Fc${\varepsilon}$RI-mediated responses, little is known about the precious mechanism for the expression of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in mast cells. Here, we report that rapamycin, a specific inhibitor of mammalian target of rapamycin (mTOR), reduces the expression of TNF-${\alpha}$ in rat basophilic leukemia (RBL-2H3) cells. IgE or specific antigen stimulation of RBL-2H3 cells increases the expression of TNF-${\alpha}$ and activates various signaling molecules including S6K1, Akt and p38 MAPK. Rapamycin specifically inhibits antigeninduced TNF-${\alpha}$ mRNA level, while other kinase inhibitors have no effect on TNF-${\alpha}$ mRNA level. These data indicate that mTOR signaling pathway is the main regulation mechanism for antigen-induced TNF-${\alpha}$ expression. TNF-${\alpha}$ mRNA stability analysis using reporter construct containing TNF-${\alpha}$ adenylate/uridylate-rich elements (AREs) shows that rapamycin destabilizes TNF-${\alpha}$ mRNA via regulating the AU-rich element of TNF-${\alpha}$ mRNA. The antigen-induced activation of S6K1 is inhibited by specific kinase inhibitors including mTOR, PI3K, PKC and $Ca^{2+}$chelator inhibitor, while TNF-${\alpha}$ mRNA level is reduced only by rapamycin treatment. These data suggest that the effects of rapamycin on the expression of TNF-${\alpha}$ mRNA are not mediated by S6K1 but regulated by mTOR. Taken together, our results reveal that mTOR signaling pathway is a novel regulation mechanism for antigen-induced TNF-${\alpha}$ expression in RBL-2H3 cells.

• Korean Journal of Animal Reproduction
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• v.23 no.4
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• pp.323-331
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• 1999

The effects of insulin-like growth factor-I (IGF-I) and cumulus cells during in vitro maturation in porcine oocytes were examined. When follicular oocytes were cultured in medium with different concentrations of IGF-I, maturation rates were 60, 61 and 62 and 72% for 0, 15 and l0ng/$m\ell$ IGF-I. In medium with 10ng/$m\ell$ IGF-I, maturation rates were not significantly difference between oocytes with (68%) and without (52%) cumulus cells during the culture. In medium with-out IGF-I, however, the maturation rates in oocytes with cumulus cells (63%) was significantly (P＜0.05) higher than oocytes without cumulus cells (32%). On the other hand, when IGF-I was added for first 24 h period or later 24 h period of culture, maturation rates were higher in oocytes with (61 and 49%) that than without (49 and 45%) cumulus cells. In experiment used medium without fetal calf serum (FCS) and porcine follicular fluid (PFF), the maturation rates in oocytes with cumulus cells for 48 h (48 and 67%) or first 24 h (46 and 63%) period after culture were significantly (P＜0.01) higher than in oocytes without cumulus cells (16 and 18%) in the presence or absence of IGF-I. These results indicated that cumulus cells is essential on maturation in vitro in porcine oocytes, but IGF-I can promote oocytes maturation of oocytes without cumulus cells in medium with FCS and PFF.

• The Journal of Pediatrics of Korean Medicine
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• v.18 no.2
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• pp.209-223
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• 2004

Objective : This study was performed to investigate the knowledge, concerns, attitudes of mothers about febrile convulsion in order to suggest education programs. Methods : The subjects of questionnaire investigation were 142 mothers visited pediatrics in Gyeongju dongguk oriental hospital between 3 september and 16 December 2004 with child under 5 years of age. Results : The rate of incorrect answer was 46.0%, experienced mothers had significant high scores than non-experienced mothers in knowledge. Mothers believed reccurent febrile convulsion will cause brain damage(88.7%), considered every febrile convulsion child will have another FC(68.3%). Mothers anticipated inserting object into the child's mouth(72.5%), rushing the child to the hospital(67.6%) during convulsion. Mothers concerned about the risk of subsequent epilepsy(70%) and experienced mothers measured the child's body temperature more frequently than before(66.7%). Conclusions : Answered mothers had inadequate knowledge, high concerns, and didn't know proper management. This suggests that enough information is needed to mothers and proper education programs should be developed in febrile convulsion.

• Journal of Advanced Marine Engineering and Technology
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• v.36 no.1
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• pp.64-71
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• 2012

An efficient cooling system is an essential part of the electronic packaging such as a high-luminance LED lighting. A special technology, Pulsating Heat Pipe (PHP), can be applied to improve cooling of a sealed, explosion-proof LED light fixture. In this paper, the characteristics of the pulsating heat pipes in the imposed thermal boundary conditions of LED lightings were experimentally investigated and a PHP device that works free of alignment angle was investigated for cooling of explosion-proof LED lights. Five working fluids of ethanol, FC-72, R-123, water, and acetone were chosen for comparison. The experimental pulsating heat pipe was made of copper tubes of internal diameter of 2.1 mm, 26 turns. A variable heat source of electric heater and an array of cooling fins were attached to the pulsating heat pipe. For the alignment of the heating part at bottom, an optimum charging ratio (liquid fluid volume to total volume) was about 50% for most of the fluids and water showed the highest heat transfer performance. For the alignment of the heating part on top, however, only R-123 worked in an un-looped construction. This unique advantage of R-123 is attributed to its high vapor pressure gradient. Applying these findings, a cooling device for an explosion-proof type of LED light rated 30 W was constructed and tested successfully.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.5
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• pp.581-588
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• 2014

Extensive researches toward pool boiling characteristics have been widely investigated. However, the correct understanding of its boiling crisis by Critical Heat Flux (CHF) phenomenon during steady and transient heat transfer as a fundamental database for designing heat generation systems is still need to be clarified. The pool boiling CHFs were investigated to clarify the generalized phenomena of transition to film boiling at transient condition. The CHFs were measured on 1.0 mm diameter horizontal cylinder of platinum for exponential heat generation rates with various periods for saturated liquids at atmospheric pressure. The incipience of boiling processes was completely different depending on pre-pressurization. Also, the dependence of pre-pressure in transient CHFs changed due to the wettability of boiling liquids. The trend of typical CHFs were clearly divided into the first, second and third groups for long, short and intermediate periods, respectively. By the effect of pre-pressurization, the boiling incipience mechanism was replaced from that by active cavities entraining vapor to that by the HSN in originally flooded cavies.

• Journal of Mechanical Science and Technology
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• v.15 no.1
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• pp.98-107
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• 2001

Continuing efforts to achieve increased circuit performance in electronic package have resulted in higher power density at chip and module level. As a result, the thermal management of electronic package has been important in maintaining or improving the reliability of the component. An experimental investigation of thermosyphonic boiling in vertical tube and channel made by two parallel rectangular plates was carried out in this study for possible application of the direct immersion cooling. Fluorinert FC-72 as a working fluid was used in this experiment. Asymmetric heated channel of open periphery with gap size of 1, 2, 4 and 26mm and uniformly heated vertical tubes with diameter of 9, 15 and 20mm were boiled at saturated condition. The boiling curves from tested surfaces exhibited the boiling hysteresis. It was also found that the gap size is not a significant parameter for the thermosyphonic boiling heat transfer with this Fluorinert. Rather pool boiling characteristics appeared for larger gap size and tube diameter. The heat transfer coefficients measured were also compared with the calculation results by Chens correlation.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.25 no.10
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• pp.830-836
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• 2012

An efficient cooling system is essential for the electronic packaging such as a high-luminance LED lighting. A special heat transport technology, Pulsating Heat Pipe (PHP), can be applied to the cooling of LED lighting. In this paper, the operational characteristics of the PHP in the imposed thermal boundary conditions of LED lighting were experimentally investigated. The experimental PHP was made of copper tubes of internal diameter of 2.1 mm. The working fluids of ethanol, FC-72, water, acetone and R-123 were chosen for comparison. The results showed that an optimum range of charging ratio exists for high cooling performance; 50% for most of the fluids. Among the five working fluids, water showed the highest heat transfer rate of 260 W. Two distinguished characteristics of pulsating direction were identified. It is also identified that high vapor pressure gradient is one of key parameters for better heat transfer performance.

• Journal of the Society of Naval Architects of Korea
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• v.31 no.1
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• pp.32-41
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• 1994

This paper presents the model-ship correlations based on model test results of 36 ships. All of model tests were conducted at KRISO towing tank The correlation factors $C_P,\;C_N,\;and\;C_{NP}$ are estimated by the ITTC Standard Method and compared with the results of another towing tank. In the 36 ships, the block coefficients of thirty ships are greater than 0.72. Nevertheless the comparison of factors is in good agreement. The corrections to the scale effect on wake fraction ${\Delta}{\omega}_c$ and roughness allowance $C_{Ac}$ are subject matter in practice. The correction formulae are proposed by functions of ship length and form factor. And the correction formula of resistance coefficient ${\Delta}C_{Fc}$ based on Townsis's hull roughness formula is presented.

• Food Science of Animal Resources
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• v.39 no.1
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• pp.65-72
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• 2019

This study investigated the effects of temperature ($50^{\circ}C$, $55^{\circ}C$, and $60^{\circ}C$) and time (12 and 24 h) on the cookery properties of sous-vide (SV) processed pork loin. As an indicator of cookery properties, cooking loss, expressible moisture (EM), pH, differential scanning calorimetry (DSC), shear force, total plate count (TPC) and CIE color were measured and compared with fresh (FC) and cooked control (CC, $75^{\circ}C$ for 30 min). SV treatments at $50^{\circ}C$ showed higher tenderness and lower cooking loss comparing to CC. DSC result indicated that thermal transition of collagen was a key factor affecting the cooking loss and shear force of meat. In comparison of CC, risks of insufficient pasteurization and uncooked color generation were not shown in SV processed meat. Therefore, the results indicated that SV had a potential advantage to produce tender and moist meat, and the best SV condition of pork loin was estimated at $50^{\circ}C$ for 24 h.

• Nuclear Engineering and Technology
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• v.56 no.1
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• pp.265-274
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• 2024

The current research focuses on the development of a numerical approach to forecast strongly subcooled flow boiling of FC-72 as the refrigerant in various vertical minichannel shapes for high-heat-flux cooling applications. The simulations are carried out using the Volume of Fluid method with the Lee phase change model, which revealed some inherent flaws in multiphase flows that are primarily due to an insufficient interpretation of shearlift force on bubbles and conjugate heat transfer against the walls. A user-defined function (UDF) is used to provide specific information about this noticeable effect. The influence of shape and the inlet mass fluxes on the flow patterns, heat transfer, and pressure drop characteristics are discussed. The computational results are validated with experimental measurements, where excellent agreements are found that prove the efficiency of the present numerical model. The findings demonstrate that the heat transfer coefficient decreases as the mass flux increases and that the constriction design improves the thermal performance by 24.68% and 10.45% compared to the straight and expansion shapes, respectively. The periodic constriction sections ensure good mixing between the core and near-wall layers. In addition, a slight pressure drop penalty versus the thermal transfer benefits for the two configurations proposed is reported.