• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.377-381
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• 2005

We tried to extract bone morphogenetic protein (BMP) from the freeze-dried bovine cortical bone (FBCB) for bone graft, which were defatted with chloroform-methanol for 20 days, freeze-dried at $-80^{\circ}C$ for 7 days and sterilized by ethylene oxide gas. Two kg of FBCB were pulverized in a wheel mill to $0.5-2.0mm^3$ cubic in size. The bone particles were demineralized in 0.6N HCI for 10 days at chloroform-methanol$4^{\circ}C$ and defatted with chloroform-methanol for 6 hours at room temperature, which was going to be defatting and demineralized cortical bone (DDM). For extracting BMP, DDM was agitated continuously through 72 hours with magnetic stirrer at $4^{\circ}C$ into 12 times of volume of 6 M guanidine hydrochloride (Gdn-HCl) solution containing proteinase inhibitors to protect BMP such as 2mM N-ethylaleimide, 1mM iodoacetic acid, 1mM phenylmethylsulfonyl fluoride and a sterilizer, 1mM sodium azide. The extraction procedure was repeated for three times. All extracted solution was centrifuged at 10,000 rpm for 30 min and then, the supernatant was dialyzed with 12 times of volume of deionized water at $4^{\circ}C$ for 24-72 hours, which cut off below 6,000-8,000 molecular weight. The dialyzed specimen contained crude-BMP was centrifuged, freeze-dried, and weighted. Through these processing, we could obtained $84.9\%$ as FBCB, $17.8\%$ as DDM and $0.71\%$ as crude-BMP from the wet cortical bone without cancellous bone, marrow and muscles. The crude-BMP were obtained $68.3\%$ from the first extraction, $29.6\%$ from secondary and $2.1\%$ from tertiary, respectively. It was suggested that high yield of crude-BMP migth be explained by three-time repetition of the extraction processing for crude-BMP with Gdn-Hcl sol.

• Annual Conference of KIPS
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• 2013.11a
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• pp.1106-1109
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• 2013

미래 육군의 대대급이하 NCW 핵심은 기반통신장비인 TMMR(Tactical Multi Function Terminal)과 전투지휘를 위한 전장단말기인 대대급이하 전투지휘체계(B2CS)로 대표할 수 있다. 이중 TMMR은 음성과 데이터 통신이 가능토록 개발중인 무전기이다. 하지만, 군 무전기 특성상 은 엄폐 지역에서의 통신 보장을 위해 낮은 주파수 대역을 사용함에 따라 최대 지원 가능한 전송속도는 매우 한정적이다. 따라서 TMMR을 기반 통신장비로 활용하는 B2CS는 이 낮은 대역폭을 어떻게 효율적으로 사용할 것인가가 핵심이다. 따라서 본고에서는 이를 위해 군 운용환경에서의 TMMR 기반하 B2CS 운용 시 필요한 TMMR의 요구대역폭을 분석, 데이터 경량화 설계가 필요함을 확인하고, 이에 따른 B2CS에서 적용하고 있는 데이터 경량화 설계 방안을 제시하고자 한다.

• Journal of the Korea Institute of Military Science and Technology
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• v.11 no.2
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• pp.16-22
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• 2008

So far, Korean command post vehicles(CPs) have used voice-only radio communication among the mechanized troop units such MBTs and IFVs, etc. But digital data communication technology should be introduced to improve mission success capabilities and accommodate integrated combat capabilities through increasing SA(situation Awareness), or spatiotemporal synchronization of military operation in battle field environment. Therefore, conventional CPs, which have been operated without digital network, urgently needs to mount a battle management system which furnish with tactical information network, considering that korean new MBTs or new IFVs will be fielded soon. This study suggests some performance enhancement method which might be applicable to CPs which have a role of connecting Battalion unit to Brigade C4I system.