• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.622-626
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• 2006

Molecular characterization of Hallikar, the native cattle breed of Karnataka, was undertaken using 19 cattle specific, highly polymorphic microsatellite markers recommended by FAO. The genomic DNA was subjected to PCR amplification and alleles were resolved through six per cent denaturing PAGE with a 10 bp DNA ladder followed by silver staining. Genotyping of animals was done based on allele size. The number of alleles ranged from three to nine with allele sizes ranging from 102 bp to 294 bp. These alleles were distributed in the frequency range between 0.0306 and 0.8673 in the population. The mean observed number of alleles was $6.368{\pm}1.4225$. The mean observed and expected heterozygosities were $0.7515{\pm}0.1734$ and $0.7850{\pm}0.1381$, respectively. The high heterozygosity observed implies presence of higher genetic variability within Hallikar breed. The PIC (Polymorphism Information Content) values ranged from 0.2322 (ETH152) to 0.8654 (ETH225). The percentage of polymorphic loci obtained was 100 as all the 19 microsatellite markers were found to be polymorphic. Except for ETH152, all the other loci had high PIC values, indicating that these markers are highly informative for characterization of Hallikar breed. The population was tested for Hardy-Weinberg equilibrium at 19 microsatellite loci, and at 74 per cent of the loci the population was found to be in disequilibrium.

• Asian-Australasian Journal of Animal Sciences
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• 제29권2호
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• pp.176-183
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• 2016

A study was conducted to assess the genetic diversity among Simmental Cross cattle in West Sumatra using microsatellite DNA markers. A total of 176 individual cattle blood samples was used for obtaining DNA samples. Twelve primers of microsatellite loci as recommended by FAO were used to identify the genetic diversity of the Simmental Cross cattle population. Multiplex DNA fragment analysis method was used for allele identification. All the microsatellite loci in this study were highly polymorphic and all of the identified alleles were able to classify the cattle population into several groups based on their genetic distance. The heterozygosity values of microsatellite loci in this study ranged from 0.556 to 0.782. The polymorphism information content (PIC) value of the 12 observed loci is high (PIC>0.5). The highest PIC value in the Simmental cattle population was 0.893 (locus TGLA53), while the lowest value was 0.529 (locus BM1818). Based on the genetic distance value, the subpopulation of the Simmental Cross-Agam and the Simmental Cross-Limapuluh Kota was exceptionally close to the Simmental Purebred thus indicating that a grading-up process has taken place with the Simmental Purebred. In view of the advantages possessed by the Simmental Cross cattle and the evaluation of the genetic diversity results, a number of subpopulations in this study can be considered as the initial (base) population for the Simmental Cross cattle breeding programs in West Sumatra, Indonesia.

• Magazine of the Korean Society of Agricultural Engineers
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• 제32권1호
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• pp.72-86
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• 1990

This study was carried out to get the basic information of irrigation plans for upland crops such as the optimum irrigation level and the project water requirement. Red peppers and cucumbers were cultivated in PVC pot lysimeters filled with 60cm deep clay loam soil. Four tensiometers were installed in each pot to measure the soil water pressure head. Six levels of irrigation were used. The results obtained from this study are summarized as follows: 1.The optimum irrigation level. The irrigation level of FC-PF2.7 was found to be the optimum level for both red pepper and cucumber with respect to the yield and the weight per fruit. In case of FC-PF2.7, total ET during the irrigation period were 1005.2mm for red pepper, and 429.6mm for cucumber, respectively. 2.soil moisture extraction patterns. Average soil moisture extraction patterns (SMEP)during the irrigation period were from 1st soil layer 43% : 32% : 16% : 9% for red pepper and 39% : 34% : 15% : 12% for cucumber, respectively. The extraction ratio of the upper soils showed very large values during the early stage of growth and decreased largely during the middle stage, and became larger in the last stage. 3.The project water requirement. Among the reference crop evapotranspiration(ETo) computation methods presented by FAO, the Penman method was found to be the best. The effective rainfall was computed by a modified USDA-SCS curve number equation. Availability ratios of the total rainfall during irrigation season were 59.2% for red pepper and 48.9% for cucumber, respectively. Net project water requirement of design year are 837.3mm for red pepper. and 502.Smm for cucumber, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제50권5호
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• pp.487-493
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• 2017

Recent years have seen steadily increasing concerns about human exposure to heavy metals through seafood consumption. In Korea, the diverse dietary patterns of different bivalve shellfish necessitate tissue-specific evaluation of heavy metal contents for risk assessment. We analyzed the heavy metal contents of various tissues in scallops (Patinopecten yessoensis) and pen shells (Atrina pectinate). In both species, we found higher levels of cadmium (Cd) in the mantle than in the adductive muscle, while there was no significant difference between the two tissue types in total mercury (THg) and lead (Pb) (P<0.05). Levels of THg and Pb were acceptable by Korean standards. However, the level of Cd in pen shell mantle tissue exceeded the standard by 40%. The rate of heavy metal exposure from consumption of these species was shown to be 0.003-0.010% of the provisional tolerable weekly intake of THg and 0.312-0.600% of the provisional tolerable monthly intake (PTWI) of Cd as recommended by the Joint FAO/WHO Expert Committee on Food Additives. These findings on the tissue-specific heavy metal contents of these species will be useful in assessing the health risks of Korean dietary habits.

• Journal of Nutrition and Health
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• 제29권3호
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• pp.251-259
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• 1996

The validity of the energy data of the starch-foods and seaweeds in Korean food composition tables has been suspected due to possible differences in their chemical compositions from those of western food ingredients. Energy conversion parameters being used currently in nutrition has been derived in countries where food items re quite different from ours. This study was undertaken to determine the metabolizable energy of starch-foods and seaweeds by the method selected in preexperiment20). Cooked starch foods and seaweeds were freeze-dried and fed to Sprague Dawley rat with 200∼300g body weight to measure apparent metabolizable energy (AME) values and nitrogen-corrected AME (AMEn) values for four days after three days of preliminary period for adaptation to the diets. The AME and AMEn values of the wheat noodle were 4554.6, 4584.7, the Starch Vermicelli, 3763.4, 3855.7, the Ra myon, 4916.9, 4876.0, the Buckwheat noodle, 4469.7, 4442.0kcal/kg dry matter, the Potato, 4514.6, 4520.0 and those of the Bread, 3256.9, 3582.6, 3260.5, kcal/kg dry matter, respectively. Those of Sea tangle were 1437.3, 1631.3 and of Laver, 3126.6, 3171.3kcal/kg dry matter, resectively. When the AME values of the starch-foods and seaweeds measureed in present study were compared with energy values calculated by various conversion parameters such as Atwater's Rubner's, Sochun's and FAO's, there appeared dramatic differences indicating that for many of the food items, the latter energy values by conversion factors are hardly acceptable. These data also suggest that the existing energy conversion factors are not applicable to seaweeds and a further study is needed to obtain specific factors for the conversion to biological energy from the chemical composition of seaweeds.

• Fisheries and Aquatic Sciences
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• 제18권1호
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• pp.13-20
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• 2015

Calculated chemical scores (computed in relation to the FAO/WHO reference protein) for salmon liver protein hydrolysates indicated that all amino acids (other than methionine and threonine) were present in adequate or excess quantities; thus, the raw liver material is a good source of essential amino acids. The hydrophobic amino acids contents in hydrolysates prepared from Oncorhynchus keta and O. gorbuscha were 38.4 and 39.1%, respectively. The proportion of released peptides exceeding 500 kDa was reduced when hydrolysates were treated with the commercial enzyme Alcalase, although proportions in the following MW ranges were elevated: 100-500 kDa and <50 kDa. The optimal conditions for enzymatic hydrolysis were as follows: pH 7.0, $50^{\circ}C$, and a reaction time of 1 h. Of the different proteases tested, Alcalase was the most efficient for production of salmon liver hydrolysate with the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. The hydrolysates prepared from salmon liver had a balanced amino acid composition. The liver protein hydrolysates contained low molecular weight peptides, some of which may be bio-active; this bio-active potential should be investigated. Inhibition of the DPPH radical increased with increased degree of hydrolysis (DH), regardless of protease type. DPPH radical scavenging abilities, antithrombotic effects and ${\alpha}$-glucosidase enzyme inhibition effects of O. keta liver hydrolysate increased in a dose-dependent manner. Thus, salmon liver hydrolysate may be useful in functional food applications and as a source of novel products.

• Journal of Korean Society of Industrial and Systems Engineering
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• 제31권1호
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• pp.116-123
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• 2008

Food and Agriculture Organization (FAO) and many countries make an effort to conserve and utilization of animal genetic resources to prepare for our unpredictable future. In order to protect the customer and the producer from the animal diseases and unjust distribution, many country seek to appropriate solution. Among the solutions, RFID technology can be used as a basic technology, since this technology can be applied in the conservancy and utilization of animal genetic resources, object management for improved animal and traceability of animal's distribution flow. There are two main issues in making the efficient RFID environment. The first issue is the standardization of code system for object identification. International Organization for Standard (ISO) published the standard which regulates RFID of animal (ISO 11784 and ISO 11785). Based on these standards, many countries have tried to establish their national standard. In Korea, National Institute of Animal Science (NIAS) playa main role in establishing the standard of object identification code based on ISO 11784. Even though the standard format of object's identification is well established, the RFID system may not be operated well without the standardization of RFID network and related equipment. In Korea, National Internet Development Agency of Korea (NIDA) has proposed the RFID Network at 2006, which can be applied in the different kind of system at each phase. But, the implementation case of this RFID Network does not reported yet, since many company or agency who introduce RFID technology, implemented as an isolated individual system. In our study, we show that RFID network can be utilized for any kind of system at each phase, and propose the improvement point in order to be widely used.

• Proceedings of the Korean Society of Crop Science Conference
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.39-39
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• 2017

In Asia, sweet potato (Ipomoea batatas) is a very important crop for starch production. Approximately 74.3% of the total sweet potato production quantity is produced in Asia (FAO, 2014) and China is the largest producer of sweet potato. Post-harvest management is particularly important because it is difficult to maintain the quality as well as quantity of sweet potatoes. Despite the importance of post-harvest management, researches on sweet potato have been focused on production-related study such as breeding of new variety, improved techniques of cultivation, so there is limited research on storage after harvest. Curing is a normal practice after sweet potato harvest to promote wound healing and extend postharvest storage life. In Korea, harvested sweet potatoes are usually cured for 4 to 7 days at $30-33^{\circ}C$ and 80-95% relative humidity within one week. Since the optimum storage temperature of sweet potato is regarded as $15-20^{\circ}C$, additional facilities and costs are required to raise the temperature for curing. However, the majority of small farmers do not have the capacity to provide additional facilities and costs. This study was initiated to suggest a new curing method to accelerate the wound healing by applying chemical oxidation to the wound surface of sweet potato. Oxidative stress is known to play an important role in the synthesis of secondary metabolites including lignin. In addition, chemical oxidation can be applied to prevent spoilage caused by microorganisms. Powerful gaseous oxidant with excellent penetration ability and superior sterilization effect was selected for this study. Lignification, weight loss, and spoilage rate of artificially wounded sweet potatoes were investigated after oxidant fumigation. There were clear differences in morphological analysis such as lignification pattern, lignin deposition color, and continuity of lignified cell layers between oxidant-fumigated sweet potatoes and control. These results show that gaseous oxidant can be used to supplement or replace the curing practice, to improve shelf-life as well as curing cost reduction.

• Journal of Environmental Health Sciences
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• 제32권2호
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• pp.186-191
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• 2006

Metal contamination of medicinal herbs is of growing concern because of their potential adverse health effects. In this study, metal exposures were estimated and their potential health risks were preliminarily evaluated using available data including metal contamination levels and consumption estimates of herbal medicine in Korea. Consumption and contamination data of 34 medicinal herbs abundantly used in Oriental medicine in Korea were used in this study. Lead, mercury, arsenic, cadmium, cobalt, and chrome were identified as contaminants of potential health concerns. Even based on a conservative exposure scenario, i.e., consuming 5 times more herbal medicine with 95th percentile contamination levels, health risks associated with herbal medicine consumption were estimated to be minuscule. Herbal consumption was 0.3% of the provisional tolerable daily intake levels recommended by Joint Expert Committee on Food Additives (JECFA) of WHO/FAO. However, it should be noted that there are several important assumptions and uncertainties associated with this evaluation: This study was conducted for only 34 types of medicinal herbs of which consumption and metal contamination data were available. In addition, there are no reliable herbal medicine consumption data among Korean population. The pattern and amount of herbal (medicine) consumption in Korea need to be investigated in order to conduct more refined risk assessment associated with metal contamination in medicinal herbs.

• Asian-Australasian Journal of Animal Sciences
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• 제27권10호
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• pp.1399-1405
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• 2014

The effective management of endangered animal genetic resources is one of the most important concerns of modern breeding. Evaluation of genetic diversity and relationship of local breeds is an important factor towards the identification of unique and valuable genetic resources. This study aimed to analyze the genetic diversity and population structure of six Korean native chicken breeds (n = 300), which were compared with three imported breeds in Korea (n = 150). For the analysis of genetic diversity, 30 microsatellite markers from FAO/ISAG recommended diversity panel or previously reported microsatellite markers were used. The number of alleles ranged from 2 to 15 per locus, with a mean of 8.13. The average observed heterozygosity within native breeds varied between 0.46 and 0.59. The overall heterozygote deficiency ($F_{IT}$) in native chicken was $0.234{\pm}0.025$. Over 30.7% of $F_{IT}$ was contributed by within-population deficiency ($F_{IS}$). Bayesian clustering analysis, using the STRUCTURE software suggested 9 clusters. This study may provide the background for future studies to identify the genetic uniqueness of the Korean native chicken breeds.