• Title/Summary/Keyword: FAME

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Low algal diversity systems are a promising method for biodiesel production in wastewater fed open reactors

  • Bhattacharjee, Meenakshi;Siemann, Evan
    • ALGAE
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    • v.30 no.1
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    • pp.67-79
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    • 2015
  • Planktivorous fish which limit zooplankton grazing have been predicted to increase algal biodiesel production in wastewater fed open reactors. In addition, tanks with higher algal diversity have been predicted to be more stable, more productive, and to more fully remove nutrients from wastewater. To test these predictions, we conducted a 14-week experiment in Houston, TX using twelve 2,270-L open tanks continuously supplied with wastewater. Tanks received algal composition (monocultures or diverse assemblage) and trophic (fish or no fish) treatments in a full-factorial design. Monocultures produced more algal and fatty acid methyl ester (FAME) mass than diverse tanks. More than 80% of lipids were converted to FAME indicating potentially high production for conversion to biodiesel (up to $0.9T\;ha^{-1}y^{-1}$). Prolific algal growth lowered temperature and levels of total dissolved solids in the tanks and increased pH and dissolved oxygen compared to supply water. Algae in the tanks removed 91% of nitrate-N and 53% of phosphorus from wastewater. Monocultures were not invaded by other algal species. Fish did not affect any variables. Our results indicated that algae can be grown in open tank bioreactors using wastewater as a nutrient source. The stable productivity of monocultures suggests that this may be a viable production method to procure algal biomass for biodiesel production.

Kinetics of the KOH Catalyzed-Methanolysis for Biodiesel Production from Fat of Tra Catfish

  • Huong, Le Thi Thanh;Tan, Phan Minh;Hoa, Tran Thi Viet;Lee, Soo
    • Journal of the Korean Applied Science and Technology
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    • v.25 no.4
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    • pp.418-428
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    • 2008
  • Transesterification of fat of Tra catfish with methanol in the presence of the KOH catalyst yields fatty acid methyl esters (FAME) and glycerol (GL). The effects of the reaction temperature and reaction time on rate constants and kinetic order were investigated. Three regions were observed. In the initial stage, the immiscibility of the Tra fat and methanol limited the reaction rate, hence this region was controlled by the mass transfer. Subsequent to this region, produced FAME like a co-solvent made the reaction mixture homogeneous, therefore the conversion rate increased rapidly so it was controlled by the kinetic parameters of the reaction until the equilibrium was approached in the final slow region. A second-order kinetic mechanism was proposed involving second regions for the forward reaction. The rate determining step for the overall KOH catalyzed-methanolysis of Tra fat was the conversion of triglycerides (TG) to diglycerides (DG). This rate constant was increased from 0.003 to $0.019min^{-1}$ when the reaction temperature was increased from 35 to $60^{\circ}C$. Its calculated activation energy was 14.379 ($kcal.mol^{-1}$).

Production Biodiesel via In-situ Transesterification from Chlorella sp. using Microwave with Base Catalyst

  • Kalsum, Ummu;Kusuma, Heri Septya;Roesyadi, Achmad;Mahfud, Mahfud
    • Korean Chemical Engineering Research
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    • v.56 no.5
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    • pp.773-778
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    • 2018
  • In-situ transesterification of microalgae lipids using microwave irradiation has potential to simplify and accelerate biodiesel production, as it minimizes production cost and reaction time by direct transesterification of microalgae into biodiesel with microwave as a heating source. This study was conducted to research the effect of microwave irradiation with in-situ transesterification of microalgae under base catalyst condition. The process variables (reaction time, solvent ratio, microwave power) were studied using 2% of catalyst concentration. The maximum yield of FAME was obtained at about 32.18% at the reaction time of 30 min with biomass-methanol ratio 1:12 (w/v) and microwave power of 450 W. The GC MS analysis obtained that the main component of FAME from microalgal oils (or lipids) was palmitic acid, stearic acid and oleic acid. The results show that microwaves can be used as a heating source to synthesize biodiesel from microalgae in terms of major components resulting.

groES Expression Related to Antifungal Activity of Streptomyces sp. SAR01 (Streptomyces sp. SAR01 균주에서의 항진균 관련 groES의 발현)

  • 이영근;김재성;조규성;장병일;추철형
    • Korean Journal of Microbiology
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    • v.38 no.3
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    • pp.162-167
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    • 2002
  • To analyse proteins and gene related to antifungal activity, SAR01 strain was isolated from a brown seaweed and identified as Streptomyces sp. by FAME(fatty acid methyl ester) analysis. Antifungal activity deficient mutant(SAR535) of Streptomyces sp. SAR01 was induced by gamma radiation$({60}^Co)$. It was found that 6 specific protein spots appeared only in SAR01 by 2-D electrophoresis analysis. Among them, a protein of 10 kDa had homology of 96% with 10 kD chaperonin cpn 10 (GroES) by Basic Local Alignment Search Tool(BLAST, NCBI) analysis. SAR535 transformants into which groES was transferred by electroporation revealed antifungal activity newly similar with SAR01 It suggested that groES be supposed to be related to the antifungal activity of Streptomyces sp. SAR01.

A Study on the Consumer Factors Affecting Private Brand Proneness (Private Brand 제품구매에 미치는 영향 연구)

  • Yun, Cha-Young;Kwon, Young-Sang
    • Journal of Industrial Convergence
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    • v.2 no.1
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    • pp.159-186
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    • 2004
  • One of the biggest change in the distributive market recently is that the development of private brand is progress actively since consumers purchasing behavior is tend to shift to resonable and practical planned purchase from brand image or brand name. Once the development of private brands is done successfully, the independent merchandising distinguished from other distribution companies is established, store image through customer satisfaction is strengthen and volume of sales and profit are increased. However, the existing studies in this field have not dealt with this matter systematically. Therefore, the purpose of this study is to propose an integrated framework within which to view private brand proneness. Factors found to influence private brand proneness included perceived cost, with private brand, perceived value for money, store fame, perceived risk, perceived quality variation between national and private brand products. Foe empirical analysis, the data were collected from 211 consumers who purchased the private brands in discount store and department store. With the collected survey data, reliability test, factor analysis for validity test and regression analysis for hypotheses test were conducted. Most hypotheses were accepted but on hypotheses were rejected negative relation of perceived cost and private brand proneness. Research results indicate that private brand proneness is affected by perceived value, perceived fame and there are negative relation between private brand proneness and perceived quality variation.

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Mixotrophic Cultivation of Marine Alga Tetraselmis sp. Using Glycerol and Its Effects on the Characteristics of Produced Biodiesel

  • Dang, Nhat Minh;Kim, Garam;Lee, Kisay
    • Applied Chemistry for Engineering
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    • v.33 no.2
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    • pp.222-228
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    • 2022
  • As a possible feedstock for biodiesel, the marine green alga Tetraselmis sp. was cultivated under different conditions of phototrophic, mixotrophic and heterotrophic cultures. Glycerol, a byproduct from biodiesel production process, was used as the carbon source of mixotrophic and heterotrophic culture. The effects of glycerol supply and nitrate-repletion were compared for different trophic conditions. Mixotrophic cultivation exhibited higher biomass productivity than that of phototrophic and heterotrophic cultivation. Maximum lipid productivity of 55.5 mg L-1 d-1 was obtained in the mixotrophic culture with 5 g L-1 of glycerol and 8.8 mM of nitrate due to the enhancement of both biomass and lipid accumulation. The major fatty acid methyl esters (FAME) in the produced biodiesel were palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3). The degree of unsaturation was affected by different culture conditions. The biodiesel properties predicted by correlation equations based on the FAME profiles mostly complied with the specifications from the US, Europe and Korea, with the exception of the cold-filter plugging point (CFPP) criterion of Korea.

Byron's Don Juan VII-VIII: Characters' Diverse Attitudes toward Glory through War

  • Yu, Jie-Ae
    • Journal of English Language & Literature
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    • v.56 no.3
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    • pp.429-443
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    • 2010
  • The purpose of this article is to examine how Byron's Don Juan VII-VIII depicts the various facets of characters' minds and actions in taking attitude toward glory during wartime in Ismail, Turkey. It explores the multifaceted sides of their hidden intentions and military activities in the self-centered and ruthless battle. Byron investigates their diverse and unreasonable causes, which drive them to undertake their particular deeds while participating in the combat. He unfolds the complex, dark layers of man's motivations and acts in responding to such martial ideals as fame, honor, success, or triumph. By making an effective characterization of four major figures such as Suwarrow, Juan, Johnson, and the Turkish Khan, Byron, indeed, enriches the poem with a variety of their different conceptions and stances toward these remarkable achievements. While fighting in the same battle, they, interestingly, reveal strikingly different attitudes, especially in responding to the complex aspects of reputation, glory, war, manliness or fate. The article also considers how the two Cantos of Don Juan feature the ironic results of the characters' quest for glory, which bring about an extensive range of inhuman consequences. The poet accentuates the diverse, negative aftermaths of their illusionary, abusive pursuit of fame and honor. In doing so, he effectively utilizes figurative portrayals of brutal pictures to highlight the unanticipated boundaries and dreadful outcomes, which have been caused by the undesirable or irrational exercises of their freedom of choice in pursuing such self-centered desires and renown.

Study on free and bond glycerines in Biodiesel from PKO(Palm Kernel Oil) and coconut oil (PKO 및 코코넛유래 바이오디젤 중 글리세린함량 분석 방법 개선 연구)

  • Lee, Don-Min;Park, Chun-Kyu;Ha, Jong-Han;Lee, Bong-Hee
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.2
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    • pp.348-361
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    • 2015
  • To reduce the effects of greenhouse gas (GHG) emissions, the government has announced the special platform of technologies as parts of an effort to minimize global climate change, and the government distributed biodiesel since 2006 as the further efforts. Although there are some debates about some quality specifications and unbalanced of source (44% from palm oil), more than 400kton/year of biodiesel was produced in 2013. Moreover the amounts will be increased when the RFS is activated. To solve the unbalanced situation and to achieve the diversity of feeds, it is essential that many researches should be considered. Especially, free and bond glycerines are one of the important properties seriously affected to the combustion system in vehicle & cold properties. Previous method (KS M 2412) couldn't cover the biodiesel derived from lauric oil($C_{12:0}$) such as PKO (Palm Kernel Oil), Coconut oil because those compositions are lighter than other conventional biodiesel sources. In this study, we review the existed method and figure out the factors should improve to analysis the glycerine from PKO and Coconut oil biodiesel. Modifying the analysis conditions to enhance the resolution and change the internal standards to avoid the overlapped- peaks between Capric acid ME ($C_{10:0}$) and standard#1(1,2,4-butantriol). From this revised method, we could solve the restrictions of previous methods. And check the possibility of new method to analyze the glycerine in biodiesel regardless of sources.

The Effect of Soybean Oil and Waste Chicken Oil Mixing Ratio on Biodiesel Characteristics (대두유와 폐계유의 혼합비가 바이오디젤 특성에 미치는 영향)

  • Kwack, Jong Won;Kim, Tae Han
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.2
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    • pp.261-267
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    • 2017
  • The interest in biodiesel is increasing rapidly. As a result, the price of vegetable oil that is used as a raw material for biodiesel is skyrocketing. Studies of biodiesel using animal waste as a means of solving these problems are underway. Biodiesel produced from animal fat contains considerably more saturated fatty acids than that produced from vegetable oil. In addition, it has a high cetane number and a high heating value. On the other hand, the fluidity decreases at lower temperatures because of the large amount of saturated fatty acids. For the biodiesel production, waste chicken oil and soybean oil were first purified. The raw materials were mixed at various ratios from 1:9 to 9:1. The methanol / oil molar ratio was also changed from 7 mol to 15 mol. The entire reaction time was one hour. The results showed that the optimal mixing ratio of soybean oil to waste chicken oil was 3:7, and the optimal methanol / oil molar ratio was 13. Moreover, the BD yield was 90.2%, the FAME content was 96.6%, and the LAME content was 4.1%. This result satisfied the Korea Industrial Standard (KSM2413).

Comparison of Microbial Community of Orchard Soils in Gyeongnam Province (경남지역 과수원 토양 미생물 군집 비교)

  • Lee, Young-Han;Lee, Seong-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.492-497
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    • 2011
  • Soil management for orchard depends on the effects of soil microbial activities. The present study evaluated the soil microbial community of 25 orchard in Gyeongnam Province by fatty acid methyl ester (FAME) method. The average concentrations in the orchard soils were $332nmol\;g^{-1}$ of total FAMEs, $94nmol\;g^{-1}$ of bacteria, $46nmol\;g^{-1}$ of Gram-negative bacteria, $42nmol\;g^{-1}$ of Gram-positive bacteria, $4.8nmol\;g^{-1}$ of actinomycetes, $54nmol\;g^{-1}$ of fungi, and $9.1nmol\;g^{-1}$ of arbuscular mycorrhizal fungi. In addition, sandy loam soils had significantly low ratio of cy19:0 to 18:$1{\omega}7c$ compared with that of loam soils (p<0.05), indicating that microbial stress decreased. The average soil microbial communities in the orchard soils were 28.1% of bacteria, 15.9% of fungi, 13.6% of Gram-negative bacteria, 12.5% of Gram-positive bacteria, 2.8% of arbuscular mycorrhizal fungi, and 1.4% of actinomycetes. The soil microbial community of Gram-negative bacteria in peach cultivating soils was significantly higher than that of pear cultivating soils (p<0.05).