• 제목/요약/키워드: FAD

검색결과 224건 처리시간 0.025초

돌연변이 Chlamydomonas reinhardtii의 영양분 제한에 따른 지질 생산 및 지방산 조성 변화 연구 (Effect of Nutrient Limitation on Lipid Content and Fatty Acid Composition of Mutant Chlamydomonas reinhardtii)

  • 백재원;최종일
    • KSBB Journal
    • /
    • 제30권2호
    • /
    • pp.91-95
    • /
    • 2015
  • Production of biodiesel from microalgae is dependent on the microalgal lipid content and free fatty acid composition. Both lipid and free fatty acid are regulated by nutrient sources. In this study, newly developed mutant Chlamydomonas reinhardtii with higher lipid content was investigated for the effect of nutrient limitation. Nitrogen $NO_3{^{-}}$ and phosphate $PO_4{^{3-}}$ were limited for nutrient starvation during the cultivation. Under nutrient starvation, total lipid content level was increased to 27~33% and C16:0 fatty acid content constituted over 31~43% of total fatty acid. Interestingly, we also found that the expression of fatty acid desaturase (FAD7) was decreased when nutrients were starved.

유전자 알고리즘을 이용한 비균일 트래픽 환경에서의 셀 최적화 알고리즘 (Network Optimization in the Inhomogeneous Distribution Using Genetic Algorithm Traffic)

  • 박병성;한진규;최용석;조민경;박한규
    • 한국통신학회논문지
    • /
    • 제27권2B호
    • /
    • pp.137-144
    • /
    • 2002
  • 본 논문에서는 유전자 알고리즘의 진화 연산을 이용하여 기지국의 위치와 송신전력을 최적화하는 알고리즘을 구현하였다. 기지국의 위치와 송신 전력을 실수형 파라미터로 정의하며 관련된 유전 연산자를 설계하였다. 최적화의 방향은 커버리지, 송신 전력, 경제성 효율이 고려되도록 다중 목적함수를 제안하였다. 본 논문에서 구현한 알고리즘음 최적 해를 직관적으로 알 수 있는 상황에 적용하여 검증하였으며 비균일 트래픽 분포를 가정한 상황에 대해 목적함수의 가중치에 따라 최적화를 수행하였다.

암환자가족의 가족기능에 대한 조사연구 (A Study on the Family Functioning in the Family with the Cancer Patient)

  • 한숙정
    • 종양간호연구
    • /
    • 제3권2호
    • /
    • pp.155-163
    • /
    • 2003
  • This study was designed to assess the level of family function between cancer patient's families and normal families, and to seek better quality family-Mcentered nursing care. The subjects for this study were 95 ca patient's families who were selected from th university hospitals and 95 normal families who selected from 1 university hospital. The instrument used for this study was McMaster Family Assessment Device (FAD) developed by Epstein, Baldwin and Bishop (1983). The scale consisted of 53 items covering 7 areas: 'problem solving', 'communication', 'roles', 'affective responsiveness', 'affective involvement', 'behavior control', and 'general functioning'. As for data analyses, descriptive statistics, ${\chi}^2-test$, t-test, ANOVA were adopted using SAS program. The result of this study was as follows: There there was no significant difference between cancer patient's family function and normal family function. Also there was no significant difference on cancer patient's family function with regard to family characteristics, disease's characteristics, and clinical stage of cancer.

  • PDF

Presenilin-2 mutation perturbs ryanodine receptor-mediated calcium homeostasis, caspase-3 activation and increases vulnerability of PC12 cells

  • Hwang, In-Young;Shin, Im-Chul;Hwang, Dae-Youn;Kim, Young-Kyu;Yang, Ki-Hwa;Ha, Tae-Yeol;Hong, Jin-Tae
    • 한국독성학회:학술대회논문집
    • /
    • 한국독성학회 2003년도 춘계학술대회 논문집
    • /
    • pp.73-74
    • /
    • 2003
  • Familial form of Alzheimer's disease (FAD) is caused by mutations in presenilin-1 and presenilin-2 (PS2). PS1 and PS2 mutation are known to similar effects on the production of amyloid $\beta$ peptide (A$\beta$) and cause of cell death in the Alzheimer's brain. The importance of the alternation of calcium homeostasis in the neuronal cell death by PS1 mutation in a variety of experimental system has been demonstrated. (omitted)

  • PDF

A Comprehensive Review on r-Learning: Authentic r-Learning Beyond the Fad of New Educational Technology

  • Jung, Sung Eun;Han, Jeonghye
    • International journal of advanced smart convergence
    • /
    • 제9권2호
    • /
    • pp.28-37
    • /
    • 2020
  • We conducted a comprehensive review on the previous research on r-Learning. By reviewing 843 previous studies about r-Learning published from 2004 to 2015, this study investigated 1) the trend of research on r-Learning over time, 2) the characteristics of targeted students in r-Learning, 3) the educational activities implemented for r-Learning, and 4) the types of educational robots used for r-Learning. The study found that the research on r-Learning has rapidly and steadily increased and the types of educational activities and educational robots has been diversified. Relying on the findings of this review, this study suggests 1) ensuring growth in both the quality and the quantity of research on r-Learning, 2) broadening the target student population of r-Learning beyond the age-limited boundaries, 3) enhancing educational activities of r-Learning, and 4) recognizing the necessity for systematic and clear concepts of types of educational robots.

Flavobacterium meningosepticum이 생산하는 Nucleoside Oxidase의 효소학적 특성

  • 최양문;조홍연;양한철
    • 한국미생물·생명공학회지
    • /
    • 제24권5호
    • /
    • pp.579-584
    • /
    • 1996
  • The molecular weight of the purified nucleoside oxidase estimated by gel filtration column chromatography was 480,000 and the enzyme protein was composed of four nonidentical subunits (81,000, 69,000, 32,000 and 16,000). On the basis of the visible absorption spectra and the enzymatic determination of the purified enzyme, the enzyme was supposed as a hemoprotein and also a flavoprotein containing 3 moles of FAD per I mole of enzyme. The isoelectric point of the enzyme was pH 5.1. Addition of metal salts such as 1 mM SnCl$_{2}$ and PbCl$_{2}$ into an enzyme reaction solution inhibited the enzyme activity by 94 and 90%, respectively. The enzyme activity was also lost significantly by hemoenzyme inhibitors such as NaCN and NaN$_{3}$ and flavoenzyme inhibitor, acriflavine and quinacrine. The maximal nucleoside oxidase activity was observed at pH 7.0 and 55$\circ$C. The nucleoside oxidase was relatively stable in the range of pH 5.5-9.0 and below 55$\circ$C.

  • PDF

대형 틸팅패드 저어널 베어링의 비선형 지진응답 해석 (Nonlinear Analysis of Seismic Responses of Large Tilting fad Journal Bearings)

  • 김성기;김경웅
    • 한국윤활학회:학술대회논문집
    • /
    • 한국윤활학회 2003년도 학술대회지
    • /
    • pp.281-288
    • /
    • 2003
  • In this paper, seismic responses of large tilting pad journal bearings which have $3\~5$ tilting pads were numerically analyzed. The turbulent lubrication equation, the energy equation and motion equation were solved at each time step. The regime of operation of this bearing is laminar, turbulent and transitional. Also viscosity of working fluid was considered as function of only temperature and inlet pressure build-up was considered. Numerical results for a large tilting pad journal bearing showed journal center, maximum temperature, maximum pressure, friction torque. The relationship of bearing response and seismic intensity are discussed.

  • PDF

Enzymatic Properties of Intracellular Adenosine Deaminase from Nocardioides sp. J-326TK

  • Hong-Ki Jun;Tae-Sook Kim
    • Journal of Life Science
    • /
    • 제9권1호
    • /
    • pp.64-68
    • /
    • 1999
  • The properties of purified intracellular adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) of Nocardioides sp. J-326TK isolated from soil have been studied. The enzyme deaminated adenosine and 2`-deoxyadenosine and the respective {TEX}$K_{M}${/TEX} values were 4.0×{TEX}$10^{-4}${/TEX} M and 5.0× {TEX}$10^{-4}${/TEX} M, but the enzyme was not active on 8-bromoadenosine, 6-methylaminopurine riboside, ATP, ADP, 2`-AMP, 3`-AMP, 5`-AMP, dAMP, cAMP, NAD, FAD, NADP and adenine. The enzyme activity was strongly inhibited by the addition of {TEX}$Hg^{2+}${/TEX} and {TEX}$Ag^{+}${/TEX}, {TEX}$Cu^{2+}${/TEX}, {TEX}$Co^{2+}${/TEX} and {TEX}$Mn^{2+}${/TEX} also inhibited the activity but much less extent. The effect of alkyl reagents, metal chelating reagents and certain other compounds on the enzyme activity were also examined. No reagent activated the enzyme. On the contrary, the enzyme reaction was slightly inhibited by o-phenanthroline and 6-benzyladenosine.

  • PDF

60 GHz 대역용 도파관 급전 Radial Line Slot Array 안테나에 관한 연구 (A Study on the 60 GHz Band Radial Line Slot Array Antenna Fed by a Rectangular Waveguide)

  • 김용훈;채희덕;이중원;박종국;김성철;김성철
    • 한국전자파학회논문지
    • /
    • 제13권3호
    • /
    • pp.257-262
    • /
    • 2002
  • 본 논문에서 60 ㎓ 대역용 도파관 급전 radial line slot array (RLSA) 안테나를 설계, 제작하였다. Ewald합 기법과 Shanks 변환을 이용한 효율적인 수치해석으로 RLSA 설계에 필수적인 슬롯을 통한 결합 특성을 계산하였고, 또한 기존의 급전 방식과는 달리 구형 도파관-radial line 변화구조를 이용한 구형 도파관 급전 구조를 사용하였다. 직경 10cm의 크기로 제작된 원형 안테나의 측정 결과 60 ㎓에서 31 ㏈i 의 이득과 38%의 효율을 나타내었다.

The Studies of Copolymer, Poly (3-hydroxybutyrate-co-3-hydroxy valerate) Synthesis in Recombinant pha+ Escherichia coli

  • Lee, Ha-Young;Rhie, Ho-Gun
    • 한국동물학회:학술대회논문집
    • /
    • 한국동물학회 1996년도 한국생물과학협회 국제학술대회
    • /
    • pp.195.2-195
    • /
    • 1996
  • acs gene cloning was constructed by subcloning the 2.2-kb MunI-MunI restriction fragment of 638 and 639 which include acs gene from the kohara phage into the unique EcoRI site of pUC18 and pJM9131 containing the PHA biosynthesis genes. Then recombinant E. coli fadRatoC(Con) mutants containing the polyhydroxyalkanoate(PHA) biosynthesis genes are able to incoporate s significant levels of 3-hydroxyvalerate (3HV) into the copolymer [P(3HB-co-3HV)]. Quantitative determination of PHB and P(3HB-co-3HV) was performed by gas-chromatographic analysis of extracts obtained from methanolysis of lyophilized cells.

  • PDF