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A Study on the Neutron Dosimetry with LiF Thermoluminescent Dosimeters

  • Yoo, Y.S.;Kim, P.S.;Moon, P.S.
    • Nuclear Engineering and Technology
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    • v.7 no.3
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    • pp.191-198
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    • 1975
  • A study was made on the neutron dosimetry in a mixed gamma-neutron field with LiF thermoluminescent dosimeter. In order to estimate the neutron dose in a mixed field, $^{6}$ LiF and $^{7}$ LiF dosimeters were used for fast and thermal neutron doses. The over-all conversion factors for the effects of dosimeter positions were derived for personnel monitoring and the glow curves of the LiF dosimeters for neutron and gamma-ray doses were also analyzed.

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The evaluate the usefulness of various CT kernel applications by PET/CT attenuation correction (PET/CT 감쇠보정시 다양한 CT Kernel 적용에 따른 유용성 평가)

  • Lee, Jae-Young;Seong, Yong-Jun;Yoon, Seok-Hwan;Park, Chan-Rok;Lee, Hong-Jae;Noh, Kyung-Wun
    • The Korean Journal of Nuclear Medicine Technology
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    • v.21 no.2
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    • pp.37-43
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    • 2017
  • Purpose Recently PET/CT image's attenuation correction is used CTAC(Computed Tomgraphy Attenuation Correction). it can quantitative evaluation by SUV(Standard Uptake Value). This study's purpose is to evaluate SUV and to find proper CT kernel using CTAC with applied various CT kernel to PET/CT construction. Materials and Methods Biograph mCT 64 was used for the equipment. We were performed on 20 patients who had examed at our hospital from february through March 2017. Using NEMA IEC Body Phantom, The data was reconstructed PET/CT images with CTAC appiled various CT kernel. ANOVA was used to evaluated the significant difference in the result. Results The result of measuring the radioactivity concentration of Phantom was B45F 96% and B80F 6.58% against B08F CT kernel, each respectively. the SUVmax increased to B45F 0.86% and B80F 6.54% against B08F CT kernel, In case of patient's parts data, the Lung SUVmax increased to B45F 1.6% and B80F 6.6%, Liver SUVmax increased to B45F 0.7% and B80F 4.7%, and Bone SUVmax increased to B45F 1.3% and B80F 6.2%, respectively. As for parts of patient's about Standard Deviation(SD), the Lung SD increased to B45F 4.2% and B80F 15.4%, Liver SD increased to B45F 2.1% and B80F 11%, and Bone SD increased to B45F 2.3% and B80F 14.7%, respectively. There was no significant difference discovered in three CT kernel (P >.05). Conclusion When using increased noise CT kernel for PET/CT reconstruction, It tends to change both SUVmax and SD in ROI(region of interest), Due to the increase the CT kernel number, Sharp noise increased in ROI. so SUVmax and SD were highly measured, but there was no statistically significant difference. Therefore Using CT kernel of low variation of SD occur less variation of SUV.

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Crossability, Variation in Chromosome Number, and Agronomic Characters in Hybrid of Hexaploid Triticale with Tetraploid Rye (6배체 트리티케일과 4배체호밀 교잡에서의 교잡율, 염색체수 변이 및 작물학적 특성)

  • 황종진;하용웅;이홍석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.4
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    • pp.305-312
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    • 1992
  • This experiment was carried out to investigate seed set, variation in chromosome number, and agronomic characteristics of the progeny in the cross between hexaploid triticale variety, Sinkihomil(P$_1$) and tetraploid rye variety, Dooroohomil(P$_2$). Seed set rate obtained was 30.5% in the cross of Sinkihomil with Dooroohomil, whereas 3.26% in reciprocal cross using Dooroohomil as female. Alsoseed set was 8.75% in F$_1$/P$_1$, 7.20% in F$_1$/P$_2$, and 1.53% in F$_2$(=F$_1$ /F$_1$, respectively. Germination rate of crossed seed was 37% in cross of P$_1$ with P$_2$, 39.0% in F$_1$/P$_1$(BC$_1$), 50% in F$_1$/P$_2$(BC$_2$) and 43.0% in F$_1$/F$_1$(F$_2$), and 1,000 grain wight was 20.7g in the cross of P$_1$ with P$_2$, which have 41.9g and 47.7g, respectively, 24.5g in F$_1$/P$_1$, 23.6g in F$_1$/P$_2$, and 24.5g in F$_1$/F$_1$, respectively. In pollen fertility of F$_1$ plant, 69.8% turned out to be abnormal or sterile pollen grains, whereas 30.2% was fertile or normal. In meiosis of pollen mother cell of F$_1$ plant, 13.5 univalents, 8.89 bivalent and 1.24 trivalent were appeared. Somatic chromosome number of 35 in F$_1$, both 32 to 33 and 35 to 36 in F$_2$, 35 to 39 in BC$_1$ and 28 to 36 in BC$_2$ which mean producing female gamate was 14 to 18 chromosome in PMC of F$_1$ plant. Rate of fertile plant turned out to be 100% in F$_1$, 4.5% in F$_2$, 42.9% in BC$_1$, and 50.0% in BC$_2$, respectively. Number of seed set per spike appeared to be 1.17 in F$_1$ plant, 13.3 in F$_2$, 2.36 in BC$_1$, and 3.75 in BC$_2$, respectively. Days to heading of F$_1$ was intermediate, but F$_2$ was later than both parents. Plant height of F$_1$ , BC$_1$ ,and BC$_2$ was shorter than both parent, but F$_2$, longer than both parents.

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Transcription Factor E2F7 Hampers the Killing Effect of NK Cells against Colorectal Cancer Cells via Activating RAD18 Transcription

  • Bingdong Jiang;Binghua Yan;Hengjin Yang;He Geng;Peng Li
    • Journal of Microbiology and Biotechnology
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    • v.34 no.4
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    • pp.920-929
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    • 2024
  • As a pivotal defensive line against multitudinous malignant tumors, natural killer (NK) cells exist in the tumor microenvironment (TME). RAD18 E3 Ubiquitin Protein Ligase (RAD18) has been reported to foster the malignant progression of multiple cancers, but its effect on NK function has not been mined. Here, the study was designed to mine the mechanism by which RAD18 regulates the killing effect of NK cells on colorectal cancer (CRC) cells. Expression of E2F Transcription Factor 7 (E2F7) and RAD18 in CRC tissues, their correlation, binding sites, and RAD18 enrichment pathway were analyzed by bioinformatics. Expression of E2F7 and RAD18 in cells was assayed by qRT-PCR and western blot. Dual-luciferase assay and chromatin immunoprecipitation (ChIP) assay verified the regulatory relationship between E2F7 and RAD18. CCK-8 assay was utilized to assay cell viability, colony formation assay to detect cell proliferation, lactate dehydrogenase (LDH) test to assay NK cell cytotoxicity, ELISA to assay levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), and immunofluorescence to detect expression of toxic molecules perforin and granzyme B. High expression of RAD18 and E2F7 was found in CRC tissues and cells. Silencing RAD18 could hamper the proliferation of CRC cells, foster viability and cytotoxicity of NK cells, and increase the secretion of GM-CSF, TNF-α, IFN-γ as well as the expression of perforin and granzyme B. Additionally, ChIP and dual-luciferase reporter assay ascertained the binding relationship between RAD18 promoter region and E2F7. E2F7 could activate the transcription of RAD18, and silencing RAD18 reversed the inhibitory effect of E2F7 overexpression on NK cell killing. This work clarified the inhibitory effect of the E2F7/RAD18 axis on NK cell killing in CRC, and proffered a new direction for immunotherapy of CRC in targeted immune microenvironment.

Synthesis of 1N-aryl-2-methyl-3-ethoxycarbonyl-pyridino [2,3-f]indole-4,9-dione derivatives (II) (1N-아릴-2-메틸-3-에톡시카르보닐-피리디노 [2,3-f]인돌-4,9-디온 유도체의 합성 (II))

  • Suh, Myung-Eun;Park, Hee-Kyung
    • YAKHAK HOEJI
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    • v.41 no.5
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    • pp.582-587
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    • 1997
  • The 6.7-dichloroquinoline-5,8-dione (I) was reacted with ethyl acetoacetate in the presence of sodium ethoxide to yield 6-(${\alpha}$-acetyl-${\alpha}$-ethoxycarbonyl methyl)-7-chloro-qui noline-5,8-dione(II). When this compound II was reacted with some arylamine (phenyl, p-toluyl, p-fluorophenyl, p-chlorophenyl. p-bromophenyl, p-iodophenyl, p-trifluoromethylphenyl, p-dimethylaminophenyl,indanyl), 1N-aryl-2-methyl-3-ethoxycarbonyl pyridino[2,3-f]-indole-4.9-dione(IIIa-I) were obtained via intramolecular cyclization.

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Effects of a Tetramethoxyhydroxyflavone on the Expression of Inflammatory Mediators in LPS-Treated Human Synovial Fibroblast and Macrophage Cells

  • Yoon, Do-Young;Cho, Min-Chul;Kim, Jung-Hee;Kim, Eun-Jin;Kang, Jeong-Woo;Seo, Eun-Hee;Shim, Jung-Hyun;Kim, Soo-Hyun;Lee, Hee-Gu;Oh, Goo-Taeg;Hong, Jin-Tae;Park, Joo-Won;Kim, Jong-Wan
    • Journal of Microbiology and Biotechnology
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    • v.18 no.4
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    • pp.686-694
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    • 2008
  • The inhibitory effects of 5,6,3',5'-tetramethoxy 7,4'-hydroxyflavone (labeled as p7F) were elucidated on the productions of proinflammatory cytokines as well as inflammatory mediators in human synovial fibroblasts and macrophage cells. p7F inhibited IL-1${\beta}$ or TNF-${\alpha}$ induced expressions of inflammatory mediators (ICAM-1, COX-2, and iNOS). p7F also inhibited LPS-induced productions of nitric oxide and prostaglandin $E_2$ in RAW 264.7 cells. In order to investigate whether p7F would inhibit IL-1 signaling, p7F was added to the D10S Th2 cell line (which is responsive to only IL-1${\beta}$ and thus proliferates), revealing that p7F inhibited IL-1${\beta}$-induced proliferation of D10S Th2 cells in a dose-response manner. A flow cytometric analysis revealed that p7F reduced the intracellular level of free radical oxygen species in RAW 264.7 cells treated with hydrogen peroxide. p7F inhibited IkB degradation and NF-${\kappa}$B activation in macrophage cells treated with LPS, supporting that p7F could inhibit signaling mediated via toll-like receptor. Taken together, p7F has inhibitory effects on LPS-induced productions of inflammatory mediators on human synovial fibroblasts and macrophage cells and thus has the potential to be an anti-inflammatory agent for inhibiting inflammatory responses.

A Development of Macroscopic Simulation Model for Interrupted Flow using Shockwave (충격파를 이용한 거시적 단속류 시뮬레이션 모형개발)

  • Lee, Ho-Sang;Jung, Young-Je;Kim, Young-Chan;Kim, Dae-Ho
    • Journal of Korean Society of Transportation
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    • v.26 no.1
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    • pp.191-201
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    • 2008
  • It has been employed TRANSYT-7F and NETSIM to evaluate the validity and effectiveness of improvement on TSM(Transportation Systems Management). But T7F is hard to describe platoon compression and dispersion in actually, and NETSIM takes a long time for network coding, calibration and have difficulty in setting up saturation flow. While Shockwave Model have advantage which can describe platoon compression and dispersion in actually and shorten hours, convenience of application. But Shockwave Model apply unrealistic traffic flow relation ship(U-K curve) and simplify platoon because of difficulty in calculating shockwave's position and cross. For solving limitation of existing shockwave models, It develop new model with 2-regime linear model, New platoon model, Extended shockwave, etc. For verifying the validity of the proposed model, it was compared with delay of T7F and NETSIM by offset variation. In conclusion, it is thought that proposed model have outstanding performance to simulate traffic phenomenon.

LINC00174 Facilitates Proliferation and Migration of Colorectal Cancer Cells via MiR-3127-5p/ E2F7 Axis

  • Ma, Yuhong;Li, Yuzhen;Tang, Yuanyuan;Tang, Ning;Wang, Dengke;Li, Xiaofei
    • Journal of Microbiology and Biotechnology
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    • v.31 no.8
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    • pp.1098-1108
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    • 2021
  • The literature indicates that LINC00174 promotes the growth of colorectal cancer (CRC) cells, but its research needs to be enriched. We tried to explore the function and mechanism of LINC00174 in CRC cell proliferation and migration. Bioinformatics analysis predicted the binding relationship and expressions of lncRNA, miRNA and mRNA. Clinical study analyzes the relationship between LINC00174 and clinical data characteristics of CRC patients. The expressions of LINC00174, miR-3127-5p and E2F7 were verified by RT-qPCR, and the combination of the two was verified by dual luciferase analysis and RNA immunoprecipitation as needed. Western blot was used to detect the expression of EMT-related protein and E2F7 protein. Functional experiments were used to evaluate the function of the target gene on CRC cells. LINC00174 was up-regulated in CRC clinical samples and cells and was related to the clinical characteristics of CRC patients. High-expression of LINC00174, contrary to the effect of siLINC00174, promoted cell viability, proliferation, migration and invasion, up-regulated the expressions of N-Cadherin, Vimentin, E2F7, and inhibited the expression of E-Cadherin. MiR-3127-5p was one of the targeted miRNAs of LINC00174 and was down-regulated in CRC samples. In addition, miR-3127-5p mimic partially reversed the malignant phenotype of CRC cells induced by LINC00174. Besides, E2F7 was a target gene of miR-3127-5p, and LINC00174 repressed miR-3127-5p to regulate E2F7. Our research reveals that LINC00174 affected the biological characteristics of CRC cells through regulated miR-3127-5p/ E2F7 axis.

Eu3+ Luminescence in Two-Dimensional Sr2-2xEuxKxSnO4 with K2NiF4 Structure (2차원적 K2NiF4형 구조의 Sr2-2xEuxKxSnO4에서 Eu3+ 이온의 Luminescence)

  • Yo, Chul-Hyun;Minh Chau, P.T.;Ryu, Kwang-Hyun;Kim, Anh-T.
    • Journal of the Korean Chemical Society
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    • v.41 no.4
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    • pp.175-179
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    • 1997
  • Luminescence of $Eu^{3+}$ has been studied in $Sr_{2-2x}Eu_xK_xSnO_4$ with two-dimensional $K_2NiF_4$ structure. The $^5D_o$$^7F_o$$Eu^{3+}$ ion represents the J=0 → J=0 transition which is forbidden by a Judd-Ofelt selection rule for electric dipole transition in 4f shell of $Eu^{3+}$ ions. However, the emission line of $^5D_o$$^7F_o$$Eu^{3+}$ emission spectra of Sr2-2xEuxKxSnO4$Sr_{2-2x}Eu_xK_xSnO_4$structure around $Eu^{3+}$ ions.

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The Optimization of Left-Turn Phase Sequences on Signalized Arterials for Minimum Delay (지체최소화를 위한 도시간선도로 좌회전현시체계 최적화에 관한 연구)

  • 김영찬;신언교
    • Journal of Korean Society of Transportation
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    • v.15 no.1
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    • pp.157-174
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    • 1997
  • 도시간선도로의 신호시간요소를 계획하는 방법은 크게 3가지로 나뉜다. 이들은 통 과폭 최대화모형, 지체도 최소화 모형 그리고 이들 두 가지 모형의 통합모형으로 구분된다. 기존의 통합모형은 지체를 줄이기 위하여 최대 통과폭 모형인 PASSER-II로 최적화된 좌회 전현시순서를 TRANSYT-7F에 사용하는 방법이다. 그러나 통과폭 최대모형과 기존의 통합 모형은 지체를 최소화해주지 못하고, 반면에 지체도 최소화모형인 TRANSYT-7F는 좌회전 현시순서를 최적화 시켜주지 못하는 단점을 가지고 있다. 따라서 본 연구의 목적은 기존 모 형들의 갖고 있는 단점을 극복하기 위하여 좌회전현시를 최적화하면서 지체를 최소화해줄 수 있는 새로운 모형(KS_SIGNAL)을 개발하는데 있다. 개발된 모형은 MAXBAND 등과 같은 최대통과폭모형을 발전시킨 혼합정수계획법 형태를 갖는다. 평가결과에 의하면 개발된 모형 으로 최적화된 좌회전현시순서를 TRANSYT-7F에 사용할 경우 TRANSYT-7F나 PASSER-II 와 TRANSYT-7F를 통합한 기존 모형들보다 지체를 줄일 수 있는 것으로 나타났다.

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