• Bulletin of the Korean Chemical Society
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• 제32권1호
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• pp.183-185
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• 2011

Adenylate kinase-like activity and phosphotransferase-like activity from $F_1$-ATPase of Escherichia coli was revealed by $^{31}P$ NMR spectroscopy. Incubation of F1-ATPase with ADP in the presence of $Mg^{2+}$ shows the appearance of $^{31}P$ resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of $F_1$-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of $F_1$-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from $F_1$-ATPase of Escherichia coli. $^{31}P$ NMR could be a valuable tool for the investigation of phosphorous related enzyme.

• Fisheries and Aquatic Sciences
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• 제11권2호
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• pp.82-87
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• 2008

Marine fisheries are important natural resources and must be maintained, especially fish species that are important sources of food. Despite the increase in stocking programs to maintain fisheries with artificially raised fish, the genetic impact stocking has on the wild fry population has not been addressed. Genetic variation in rock bream, Oplegnathus fasciatus, within and between wild-caught parents and the $F_1$ generation produced by them in 1 day was assayed using nine highly variable micro satellite markers. The nine micro satellite loci used in this study displayed diverse polymorphisms, and in total, 98 different alleles were observed over all loci. Differences in genetic variability of the $F_1$ offspring compared to their wild-caught parents (brood stock) were observed in terms of allele frequency, gene diversity, and heterozygosity. Although the $F_1$ generation of rock bream was missing 16% of the micro satellite alleles, no significant reduction was found in mean heterozygosity of the $F_1$ population compared to the brood stock. Eight of nine loci showed significant Hardy-Weinberg equilibrium (HWE) deviations in the $F_1$ population, while the brood stock deviated from HWE at three micro satellite loci (KOF85, KOF360 and KOF374). These deviations showed mostly a deficit of heterozygotes. Our results provide evidence for genetic differences in the $F_1$ hatchery offspring compared to their wild-caught parents and reinforce the need for a series of consecutive egg collections to avoid the loss of genetic variability. This also further underscores the importance of monitoring genetic variability of hatchery populations for the conservation of natural rock bream resources.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.161-162
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• 1989

• 한국작물학회지
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• 제36권6호
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• pp.485-495
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• 1991

트리티케일 품존육성의 기초자료를 제공하기위해, 6배체 트리티케일인 신기호밀(TC)과 2배체 호밀 2개 품종 등을 교잡한 잡종 초기세대의 교잡친화성 및 세포유전학적 양상을 검토한 시험결과를 요약하면 다음과 같다 1 신기호밀 (TC)과 2배체 호밀의 교잡에서 교잡율은 조합에 따라 39.3～41.6%로, 평균 40.5%로 나타났다. 그러나 이들의 역교배에서는(2배체 호밀$\times$신기호밀) 교잡율이 극히 낮았다. 트리티케일/2배체 호밀의 F$_1$에 호밀을 화분친으로 교잡(F$_1$/P$_2$)했을 때는 평균 5.47%, TC를 화분친으로(F$_1$/P$_1$) 했을때는 평균 2.69%의 교잡율을 보였고, F$_2$는 0.38% 였다. 모든 단교배 F$_1$ 종자의 발아율은 80% 이상이었으며 F$_1$/P$_2$ 세대는 평균 65.9%, F$_1$/P$_1$은 59.5%, F$_2$는 40.8%로 세대별로 차이가 있었다. 트리티케일과 호밀의 F$_1$ 화분 임성은 평균18.7%로 나타났다. 트리티케일과 호밀의 F$_1$에서 1가 염색체 12.6, 2가 염색체 6.94, 3가 염색체 0.53개였다. 트리티케일/호밀(TC/R)에서는 F$_1$의 염색체수는 28개였고 F$_2$는 21～34, F$_1$/P$_1$은 33～38개의 분포를 보였고 F$_1$/P$_2$은 20～21개 염색체를 갖는 개체의 빈도가 높았다.

• Current Optics and Photonics
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• 제5권4호
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• pp.458-465
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• 2021

Borate nonlinear optical crystals have been used as frequency conversion devices in many fields due to their unique transparency and nonlinearity from ultraviolet to visible spectral range. In this study, we theoretically and numerically investigate the properties of broadband second harmonic generation (SHG) in the recently reported Pna2₁-Ba₃Mg₃(BO₃)₃F₃ (BMBF) crystal. The technique is based on the simultaneous achievement of birefringence phase matching and group velocity matching between interacting waves. We discussed all factors required for broadband SHG in the BMBF in terms of two types of phase matching and group velocity matching conditions, the beam propagation direction and the corresponding effective nonlinearity and spatial walk-off, and the spectral responses. The results show that bandwidths calculated in the broadband SHG scheme are 220.90 nm (for Type I) and 165.85 nm (for Type II) in full-width-half-maximum (FWHM). The central wavelength in each case is 2047.76 nm for Type I and 1828.66 nm for Type II at room temperature. The results were compared with the non-broadband scheme at the telecom C-band.

• 한국연초학회지
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• 제20권1호
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• pp.33-39
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• 1998

Survival, growth and reproduction of the cigarette beetle, Lasiodema sewicome F., were studied on flue-cured (NC82), air-cured (Br.21), aromatic (Izmir) tobacco and artificial diet (wheat feed 95% + yeast 5%). Cigarette beetle adults occurred from first generation in the flue-cured and aromatic tobacco, but no adults emerged in air-cured tobacco after the 2nd generations. The head capsule widths of larvae of the cigarette beetles L sewicome F.1 reared on different diets at 2$0^{\circ}C$, $25^{\circ}C$ and 3$0^{\circ}C$ were measured. The head capsule development was slower at 2$0^{\circ}C$ on the same diets than $25^{\circ}C$ and 3$0^{\circ}C$, and slower in the flue-cured and aromatic tobacco than the artificial diet at 2$0^{\circ}C$ and 2S$^{\circ}C$, At $25^{\circ}C$ the larval head capsule developed more rapidly in the aromatic tobacco than the flue-cured tobacco. However, no larval head development was noticed in the air-cured tobacco. The correlation between population densities of cigarette beetle,1 sewicome F., and sugar content of tobacco leaves was positive, but negative in nicotine contents. The regression between densities and sugar content was Y : 22.79 X + 57.29 (r=0.891**), and between nicotine content was Y : -141.31X + 321.40(r=-0.917**).

• 한국연초학회지
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• 제14권2호
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• pp.126-132
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• 1992

This experiment was conducted to examine estimates of heterosis, degree of dominance, heritability and phenotypic and genotypic correlation for several mutant characters in flue-cured tobacco variety. Significant heterosis was noted, recording 2.73 and 6.16% for leaf width, -6.86 and -4.72 for leaf shape index, 19.8 and 23.4% for bacterial wilt disease index in Fl an4 F2 generation, respectively, and -1.44 for days to flower in F2 generation. And also leaf width was appeared partial dominance in Fl, and leaf shape index in Fl and F2, Leaf width in F2 was appeared overdominance. Estimated heritabilities in the broad sense ranged from 0.77 to 0.87 for plant and stalk height and days to flower, while those of leaf number, leaf length and width, leaf shape index and bacterial wilt disease index ranged from 0.50 to 0.65. And the heritabilities in the narrow sense were appeared 0.64 and 0.72 for stalk and plant height, respectively, and the others were ranged from 0.32 to 0.47 Positive genotpic correlations appeared among plant height, stalk height, leaf length and leaf width, and between leaf shape index ands day to flower. And negative genotypic correlations appeared between leaf shape index and plant height and stalk height and leaf width, and between bacterial wilt disease index and leaf length.

• 한국결정성장학회지
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• 제13권1호
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• pp.15-18
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• 2003

High purity single crystalline calcium fluoride ($CaF_2$) has excellent optical transmission characteristics down to deep UV and is therefore selected as the main optical material for the next generation of lithography apparatus operating at wavelength of 157 nm. The growth of large sized $CaF_2$ single crystals with the required properties for this optical application can be achieved only by optimizing the crystal growth process by the aid of numerical simulation. This needs especially a precise calculation of the heat transport and temperature distribution in the solid and liquid $CaF_2$ under crystal growth conditions. As $CaF_2$ is considered to be semitransparent, the internal radiative heat transfer in $CaF_2$ plays an decisive role in the simulation of the heat transport. On the other hand it is very difficult to obtain quantitative experimental data for evaluating numerical models as $CaF_2$ is extremely corrosive at high temperatures. In this work we present a newly developed experimental technique to perform temperature measurements in $CaF_2$-crystal as well as in the melt under conditions of crystal growth process. These experimental results are compared to calculated temperature data, which were obtained by using different numerical models concerning the internal heat transfer in semitransparent $CaF_2$. It will be shown, that an advanced model, which was developed by the authors, gives a much better agreement with experimental data as a standard model, which was taken from the literature.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1524-1528
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• 2005

Molecular genetic markers were used to detect chromosomal regions which contain economically important traits such as growth, carcass, and meat quality traits in pigs. A three generation resource population was constructed from a cross between Korean native boars and Landrace sows. A total of 240 F2 animals from intercross of F1 was produced. Phenotypic data on 17 traits, birth weight, body weights at 3, 5, 12, and 30 weeks of age, teat number, carcass weight, backfat thickness, body fat, backbone number, muscle pH, meat color, drip loss, cooking loss, water holding capacity, shear force, and intramuscular fat content were collected for F2 animals. Animals including grandparents (F0), parents (F1), and offspring (F2) were genotyped for 80 microsatellite markers covering from chromosome 1 to 10. Least squares regression interval mapping was used for quantitative trait loci (QTL) identification. Significance thresholds were determined by permutation tests. A total of 10 QTL were detected at 5% chromosome-wide significance levels for growth traits on SSCs 2, 4, 5, 6, and 8.

• 한국연초학회지
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• 제22권1호
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• pp.25-30
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• 2000

Bacterial wilt(Ralstonia solanacearum) is one of the major diseases of flue-cured tobacco (Nicotiana tabacum L.) in the world. This study was conducted to investigate degree of dominance, selection, and correlation between leaf shape and degree of bacterial wilt resistance in flue-cured tobacco. The degree of disease caused by bacterial wilt was evaluated in parents, F$_1$, F$_2$ and F$_3$ populations of two crosses, BY 4 x NC 95 and BY 4 x Coker 86, in the infected field. The leaf shape index was also measured in parents and F$_2$ population of BY 4 x NC 95. The incidence of bacterial wilt was observed in the middle of June and peaked in late July, when the highest value of pathogen density reached 1.0 x 10$^{6}$ colony forming unit per gram. It was concluded that the inheritance mode of risestance to bacterial wilt in the above two crosses of susceptible and resistant varieties was recessive and polygenic. The resistance to bacterial wilt was significantly correlated with leaf shape in F2 generation of BY 4 x NC 95. But certain plants having narrower leaves were also resistant to bacterial wilt. It is considered that the bacterial wilt resistant lines having narrower leaves could be selected. The selection for bacterial wilt resistance in the F$_2$ population might be effective.