• Asian-Australasian Journal of Animal Sciences
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• v.19 no.1
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• pp.55-60
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• 2006

This experiment was conducted to investigate the effects of selenium (Se) sources and levels on growth performance, nutrient digestibility and Se retention in growing-finishing pigs. A total of 56 crossbred pigs ([$Landrace{\times}Yorkshire$]${\times}$Large White) with average $28.5{\pm}0.2kg$ BW were allotted to 7 treatments on the basis of sex and weight in two replicates and four pigs per pen. A $2{\times}3$ factorial arrangement of treatments was used in a randomized complete block (RCB) design. Two sources of Se (selenite Se or Se-enriched yeast) were added at 0.1, 0.3 and 0.5 mg/kg to each treatment diet. A basal diet without Se supplementation was the seventh treatment group. Three pigs per treatment were randomly selected and samples of loin, liver, pancreas and a kidney were collected, frozen and later analyzed for Se. The digestibility trial was conducted to evaluate the apparent absorption and retention of Se and availability of other nutrients. Growth performance was not affected by dietary sources and levels of Se. No growth retardation was observed in the 0.5 mg/kg dietary Se treatment group regardless of Se sources. The Se concentration of serum in Se supplemented groups was increased compared with the control group (p<0.01). During the growing and finishing phase, Se in serum was clearly increased when organic Se was provided (p<0.01). Interaction of Se source ${\times}$ Se level was observed in Se concentration of loin, liver and pancreas of the pigs at the end of experiment. Selenium retention in the liver, kidney, pancreas and loin of pigs was increased as dietary Se level increased and was higher when pigs were fed organic Se resulting in an interaction response (p<0.01). Nutrient digestibilities were not affected by dietary Se sources or levels. No dietary Se source ${\times}$ Se level interaction was observed in nutrient digestibility. The results from this experiment indicated that dietary Se sources and levels affected the distribution of Se in the body of growing-finishing pigs. Organic source of Se, such as Se-enriched yeast resulted in higher serum and tissue Se concentration compared to inorganic form, while no beneficial effects on nutrient digestibility were observed from dietary Se supplementation in growing-finishing pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.2
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• pp.153-159
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• 2006

A 2,656 bp fragment of chicken ghrelin gene was cloned and SNPs were detected by PCR-RFLP and Allele Specific PCR (ASP) in 12 Chinese indigenous chicken breeds and a commercial chicken population. The results showed that there were 23 base variations and an amino acid change ($Gln{\rightarrow}Arg$) in cloned chicken ghrelin gene. Three SNPs were confirmed in 13 populations and associations between this gene and growth traits of Tibetan chicken (TC) and Recessive White chicken (RW) were investigated. The results of haplotype analysis revealed that 26 haplotype genotypes were composed of eight haplotypes. The results of $x^2$ tests indicated that there were significant differences between genotypes or haplotype genotype frequencies in some of the breeds or sexes at 0.05 or 0.01 levels. The results of ANOVA revealed that there were significant differences between genotypes or haplotype genotypes on some growth traits of TC and RW chicken breeds at 0.05 or 0.01 levels. Multiple comparisons showed that there were significant associations between genotype CT at site 71 and some growth traits of two chicken breeds and between genotype AG at site 1,215 and body weight at 16 wk of two chicken breeds, and there was a significant association between haplotype genotype CAA/CAG and body weight and shank girth at 16 wk of two chicken breeds.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.10
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• pp.1466-1473
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• 2013

This experiment was conducted to study the variation laws and correlations of shearing force and feed characteristics including morphological characteristic, chemical composition and in situ degradability of wheat straw. Feasibility of evaluating the nutritional value of wheat straws with shearing force values was analyzed in this study. Six hundred wheat straw plants (Jimai 22) were randomly selected and placed in a cool and ventilated place. Samples were collected in the 1st, 15th, 30th, 45th, 60th d after harvest to measure shearing force, morphological characteristic, nutritional composition. Rumen degradation of dry matter (DM), neutral detergent fiber (NDF) and acid detergent fiber (ADF) of wheat straws were determined by the nylon bags method. The results demonstrated that linear and quadratic effects of storage time on all the tested morphological characteristics were significantly correlative (p<0.01). As storage time goes on, all the tested nutrients and their rumen degradations of wheat straw was linearly (p<0.01) and quadratic (p<0.01) correlative except ADF content and rumen degradation of ADF. Significant correlations were determined in linear effect of shearing force on morphological characteristics (p<0.01), and linear density and diameter were a more sensitive predictor than stem thickness for shearing force. There were strong correlations between storage time and all the measured physical characteristics (shearing force, morphological characteristics and shearing force standardized by morphological characteristics) (p<0.01). Nutrition compositions were linearly correlative with shearing force and standardized shearing force (p<0.01). The linear correlation between rumen degradation of DM and NDF and shearing force and standardized shearing force were evident (p<0.01). In conclusion, shearing force, nutrition compositions and their rumen degradation of wheat straw were still dynamic with storage time after harvest. Correlation could be found between shearing force and nutritional characteristics of wheat straw. Nutrient content, morphological index and rumen degradation of DM and NDF could be predicted by changes in shearing force. Shearing force should be applied according to a standardized storage time when it is used to forecast the feed value of wheat straws.

• Journal of Korean Academy of Nursing
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• v.12 no.1
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• pp.1-12
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• 1982

Presently, there is increasing demand for geriatric nursing care due to increasing proportion of elderly population and extended age. And, of particular importance in deforming the effectiveness of nursing care given to the elderly is the attitude of nursing personnel toward the elderly patient. This study was intendent to investigate the attitudes of baccalaureate nursing students toward the elderly, and to investigate any possible correlation between these attitudes and the following variables: the students' levels (freshman, sophomore, junior, senior), their preferences for var-ious fields of specialization in nursing after graduation, their clinical experiences with geriatric-patients, their personal experience with elderly family members, their educational experience of the elderly's health problems and geriatric nursing, and their experience with relatives and acquaintances over 60 years of age. Therefore, the purpose of this study was to compile basic data about the attitudes of nursing students toward the elderly in order to contribute to the improvement of geriatric nursing. The subjects for this study were 259 nursing students: 163 in the College of Nursing at E-womans University., and 96 in the Department of Nursing in K. College. Data was collected from the 11th of Sep., to the 8th of Oct., 1981, using the Tuckman-Lorge Attitude Questionnaire which contained 131 items excepting 6 items. The attitudes toward the elderly was scored by the use of mean and standard deviation. The relations between attitudes toward the elderly and six independent vairables were analysed by T-test. The results which were obtained are as follows : 1. The attitude score of nursing students was M=71.67 S.D= 16.18. Nursing students agreed with 54.8% of the stereotypic statements, and they had stereotypic attitudes toward the elderly relatively. (P<0.01). 2. There was a significant difference between the attitudes of nursing students toward the elderly, and sophomores had a less stereotypic attitudes than juniors and seniors (P<0.01). 3. There was significant difference between the attitudes toward the elderly and the preferences for various fields of specialization in nursing after graduation, and nursing students those who wanted maternity fields had a more stereotypic attitudes than others (P<0.05, p<0.01). 4. There was no significant difference between the attitudes toward the elderly and the clinical experience with the geriatric patients (P>0.05). 5. There was no significant difference between the attitude3 toward the elderly and the personal experience with elderly family members (F>0.05). 6. There was no significant difference between the attitudes toward the elderly and the educational experience of the elderly's health problems and geriatric nursing (P>0.05). 7. There was no significant difference between the attitudes toward the elderly and the experience with relatives and acquaintances over 60 years of age (P>0.05).

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.40-44
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• 2001

DPPH(1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity and Vitamin contents in different plant parts and harvesting time of Rosa rugosa Thunb were determined. Among the plants parts analysed, the highest DPPH radical scavenging activity in part was root $(below\;0.5\;{\mu}g/ml)$, leaf contained the highest ascorbic acid content of 2999.6 mg/100g. Fruit contained ascorbic acid (713.6 mg/ 100g) 4.2 times less than leaf. Vitamin contents in leaf parts also differed depanding on harvesting time. Vitamin content in leaf harvested in November was much higher than that in September. Spectrophotometric analysis of total ${\beta}-carotene$ in leaf showed lower contents than that in fruit.

• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.174-185
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• 2010

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. To elucidate the functions of a large population of Chinese cabbage (Brassica rapa) genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the full-length cDNA Over-eXpresser (FOX) gene hunting system. With oligo dT column it purify the each mRNA from the flower organs, leaf and stem tissue. And about 120,000 cDNAs from the library were transformed into $\lambda$-pFLCIII-F vector. Of which 115,000 cDNAs from the library were transformed into T-DNA binary vector, pBigs for transformation study. We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. Full-length Chinese cabbage cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Selfed seeds were harvested from transgenic Arabidopsis. We had selected 2,500 transgenic plants by hygromycin antibiotic tolerant test, and obtained a number of transgenic mutants. Each transgenic Arabidopsis was investigated in morphological changes, fertility and leaf colour. As a result, 285 possible morphological mutants were identified. Introduced cDNA was isolated by PCR amplification of the genomic DNA from the transgenic mutants. Sequencing result and BLAST analysis showed that most of the introduced cDNA were complete cDNAs and functional genes. Also, we examined the effect of Bromelain on enhancing resistance to soft rot in transgenic Chinese cabbage 'Osome'. The bromelain gene identified from FOX hunting system was transformed into Chinese cabbage using Agrobacterium methods. Transformants were screened by PCR, then RT-PCR and real time PCR were performed to analyze gene expression of cysteine protease in the T1 and T2 generations. The anti-bacterial activity of bromelain was tested in Chinese cabbages infected with soft rot bacteria. The results showed that the over-expressed bromelain gene from pineapple conferred enhanced resistance to soft rot in Chinese cabbage.

• Proceedings of the Korean Society of Toxicology Conference
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• 2006.11a
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• pp.32-33
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• 2006

There is compelling evidence that chronic inflammation predisposes to biliary tract cancer. Previously we found that aspirin use and variants in the PTGS2 gene, both of which are closely linked to inflammation, were associated with biliary tract cancer risk in a population-based study in China. To test the inflammation hypothesis further, we examined the associations of variants in 20 genes involved in the inflammation pathway with risk of biliary tract cancer and stones in a large population-based case-control study in Shanghai, China. We genotyped 56 single nucleotide polymorphisms (SNPs)from 20 inflammation genes in 411 biliary tract cancer cases (237 gallbladder cancers, 127 extrahepatic bile duct cancers, and 47 ampullary cancers), 895 subjects with biliary stones, and 786 population controls. Unconditional logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals (Cls) for the association of individual SNPs and haplotypes with biliary stones and biliary tract cancer risk. Of the 56 SNPs examined, 20 showed some associations with biliary cancer and stones. Specifically, variants of the IL8, IL8RB, RNASEL, TGF-beta, and TNF-alpha genes were associated with gallstone risk, while variants in the IL1A, IL10, VEGF, and RNASEL genes were associated with gallbladder cancer risk. Adjustment for multiple comparisons did not materially change these results. Of the 10 genes with multiple SNPs, we inferred halotypes; only one haplotype in the IL8RBgene was associated with gallstones. The haplotype frequency was significantly different between bile dict cancer cases and control (p=0.007). A haplotype comprising 3 SNPs in the IL8RB gene (rs2230054, rs1126579, rs1126580) was associated with a 54% increased risk of bile duct stones (95% CI 1.14-2.07, p=0.02), relative to the most frequent haplotype. In summary, common variants in immune-related genes influencing inflammatory responeses were associated with gallstones and biliary tract cancer, lending further support to the role of inflammation in the pathogenesis of biliary stones and biliary tract cancer. Future larger studies with more complete gene coverage are needed to confirm these results.

• Nutrition Research and Practice
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• v.11 no.2
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• pp.121-129
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• 2017

BACKGROUND/OBJECTIVES: The study was conducted to evaluate the effects of dietary leucine supplementation on mitochondrial biogenesis and energy metabolism in the liver of normal birth weight (NBW) and intrauterine growth-retarded (IUGR) weanling piglets. MATERIALS/METHODS: A total of sixteen pairs of NBW and IUGR piglets from sixteen sows were selected according to their birth weight. At postnatal day 14, all piglets were weaned and fed either a control diet or a leucine-supplemented diet for 21 d. Thereafter, a $2{\times}2$ factorial experimental design was used. Each treatment consisted of eight replications with one piglet per replication. RESULTS: Compared with NBW piglets, IUGR piglets had a decreased (P < 0.05) hepatic adenosine triphosphate (ATP) content. Also, IUGR piglets exhibited reductions (P < 0.05) in the activities of hepatic mitochondrial pyruvate dehydrogenase (PDH), citrate synthase (CS), ${\alpha}$-ketoglutarate dehydrogenase (${\alpha}$-KGDH), malate dehydrogenase (MDH), and complexes I and V, along with decreases (P < 0.05) in the concentration of mitochondrial DNA (mtDNA) and the protein expression of hepatic peroxisome proliferator-activated receptor-${\gamma}$ coactivator $1{\alpha}$ (PGC-$1{\alpha}$). Dietary leucine supplementation increased (P < 0.05) the content of ATP, and the activities of CS, ${\alpha}$-KGDH, MDH, and complex V in the liver of piglets. Furthermore, compared to those fed a control diet, piglets given a leucine-supplemented diet exhibited increases (P < 0.05) in the mtDNA content and in the mRNA expressions of sirtuin 1, PGC-$1{\alpha}$, nuclear respiratory factor 1, mitochondrial transcription factor A, and ATP synthase, $H^+$ transporting, mitochondrial F1 complex, ${\beta}$ polypeptide in liver. CONCLUSIONS: Dietary leucine supplementation may exert beneficial effects on mitochondrial biogenesis and energy metabolism in NBW and IUGR weanling piglets.

• Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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• v.7
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• pp.237-256
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• 2003

Das Deutsche als Fremdsprach hat sich als akademische Disziplin etabliert; es hat seine - von vielerlei Irrungen und Wirrungen begleitete - Startphase hinter sich und ist in eine Phase der Konsolidierung eingetreten. Bei aller Unterschiedlichkeit der $Ans\"{a}tze$, Forschungsmethoden und Lemziele gibt es doch eine Einheit in der Vielfalt, was angesichts der erst wenige ]ahrzehnte alten Disziplin erstaunt. Im Zentrum stehen nach allgemeiner Auffassung die Theorie und Praxis des Lehrens und Lernens/ Erwerbens des Deutschen als Fremdsprache; unterschidliche Auffassungen bestehen vor allem daruber, ob Deutsch als Fremdsprache ein germanistisches oder aber ein fremdsprachenphilologisches Fach sei. Unbestritten sind dagegen Fremdperspektive und $Interdisziplinarit\'{a}t$ der Disziplin rnit zahlreichen Beztigen zu Nachbarwissenschaften. Eine wesentliche Rolle in der Diskussion spielte stets die Rolle der Grammatik im fremdsprachlichen Deutschunterricht. Freilich hat die Diskussion ergeben, dass dieses in Wahrheit eine Scheinaltemative ist, die auf einer Reihe falscher Annahmen oder $Missverst\"{a}ndnissen$ beruht, vor allem jenen, im Unterricht komme es $ausschlie{\ss}lich$oder zurnindest vorrangig auf Fertigkeiten und Kommunikation an, nicht oder nur am Rande auf die Vermittlung sprachsystematischer Erkenntnisse. Durchgesetzt hat sich heute freilich die Erkenntnis, dass Grammatik, verstanden in diesem weiten Begriffssinn, aus dem Fremdsprachenunterricht nicht zu verbannen ist. Weiterhin $d\"{u}rfte$ Einvemehmen dariiber herrschen, dass Grammatik und Kommunikation keinen Gegensatz darstellen, vielmehr das Lernziel kommunikative Kompetenz die Beherrschung grammatischer Regeln einbezieht, wenn nicht voraussetzt. Der Funktionsbegriff ist umstritten. Der Oberbegriffe $f\"{u}r$ alle Funktionen $w\"{a}re$ Kommunikation. Fur unser Verstandnis einer funktionalen Grammatik fur Lemende des Deutschen als Fremsprache ist die entscheidende Frage, welche Funktion in der Kommunikation eine bestimmte Struktur spielt. Das Ziel oder der Zweck der kommunikativen Handlung steht im Mittelpunkt. Wir meinen, dass auch jenseits des deutschen Sprachraums Form und Funktion des sprachlichen Zeichens eine Einheit darstellen und im Unterricht nicht getrennt werden $k\"{o}nnen$. Zuerst lernen wir die Formen und Strukturen sprachlicher Mittel kennen, und danach werden wir sie praktisch an: Regelwissen, aber Versagen in der Kommunikation. Die Beispiele der sprachlichen $M\"{o}glichkeiten$ der Satzarten unter funktionalem Aspekt und des Ausdrucks der $Modalit\"{a}t$ im Deutschen machen die Notwendigkeit eines funktional-kommunikativen Ansatzes einer Grammatik fur Deutsch als Fremdsprache deutlich. Es belegt zugleich, dass - unter dem Primat des Didaktischen - die Grenze von Grammatik und Lexikon $\"{u}berwinden$ werden muss, um den Lemenden wirkliche $Erkl\"{a}rung$ und Lernhilfen zu bieten.

• Restorative Dentistry and Endodontics
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• v.15 no.1
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• pp.57-66
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• 1990

The author conducted a dental survey of college girl students and compared with that of college students 20 years and 10 years ago which based on the same criteria. The items of this survey included the prevalence of dental caries, DMFT and the kinds of restored materials. College girl students 2,294 were examined in May 1988, with the recommended criteria and method of W.H.O.. Among them, the data of 2,243 were analyzed and compared with the data of 1968 and 1978. The following results were obtained : 1. The average number of present teeth was $28.86{\pm}1.65$, which does not show much difference when compared to $29.00{\pm}1.94$ of 1968 and $28.97{\pm}1.64$ of 1978. 2. The prevalence of dental caries was 86.22%, which increased in comparison to 83.25% of 1968 and 84.92% of 1978. 3. DMF index was 19.98, which considerably increased in comparison to 14.56 of 1968 and 15.51 of 1978. 4. The number of DMFT was $5.75{\pm}5.04$ per student. Compared to $4.23{\pm}3.88$ of 1968 and $4.49{\pm}3.69$ of 1978, there is a considerable increase. I) The average number of decayed(D) teeth was $1.14{\pm}1.63$, which decreased from $1.16{\pm}1.57$ of 1968 and $2.09{\pm}2.16$ of 1978. II) The average number of missing(M) teeth was $0.23{\pm}0.73$, which also decreased from $0.69{\pm}1.11$ of 1968 and $0.32{\pm}0.79$ of 1978. III) The average number of filled(F) teeth was $4.39{\pm}4.91$, which increased from $2.38{\pm}3.33$ of 1968 and $2.09{\pm}3.17$ of 1978. 5. According to the analysis of filling material, filling baby including amalgam, gold inlay, resin, silicate cement and paladium inlay was 90.5%, which increased in comparison to 72.8% of 1968 and 83.6% of 1978. Among them, amalgam occupied 62.93%, which showed the most increase. On the other hand, crown and bridge was 6.5%, 3.0%, respectively, which showed continuous decrease from 12.1%, 15.1% of 1968 and 8.7%, 7.7% of 1978. Therefore, the prosthodontic treatment reduced due to the decrease of tooth extraction. In contrast, there is continuous increase of conservative treatment which maintaining natural tooth.