• 한국균학회지
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• 제23권4호통권75호
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• pp.359-368
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• 1995

F. napiforme, F. beomiforme and F. nygamai could not be classified in any of the existing sections of the genus Fusarium. To discuss of the exact taxonomic relationships among these species, the cellular polypeptide patterns were compared by using 2-D PAGE. Polypeptide pattern of F. beomiforme was different from those of other two species and was more similar to F. oxysporum in section Elegans. F. nygamai and F. napiforme might be another same section which would lie between section Liseola and section Elegans. The results were consistent with the comparison of isoenzyme patterns in these species.

• 미생물학회지
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• 제29권1호
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• pp.69-73
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• 1991

The chromosome of Fusarium species during the vegetatve nuclear divisions in hyphae were observed by use of HCl-Giemsa technique on light microscope. The haploid chromosome number of Fusarium anthophilum 7472 was n=7, n=6 in F. anthophilum 7481 and n=6 in F. oxysporum 7500. The haploid chromosome number was 7 in F. napiforme 6129 and F. napiforme 6144. Those of F. caucasicum F. caucasicum ATCC 18791 and F. aquaeductuum ATCC 15612 were n=5. F. coeruleum ATCC 20088 was n=6, n=8 in F. camptoceras ATCC 16065 and n=7 in F. sambucinum NRRL 13451. From these results and previous papers, it may be concluded that the basic haploid chromosome number of the genus Fusarium is n=4.

• 한국균학회지
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• 제22권4호
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• pp.386-393
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• 1994

Fusarium 속내 종들의 분류학적 위치를 알아보기 위하여, 각 균의 $esterase-{\alpha},\;-{\beta},\;acid\;phosphatase,\;malate\;dehydrogenase,\;peroxidase,\;polygalacturonase$를 추출하여 전기영동의 방법으로 isozyme patterns를 비교하여 보았다. Banding patterns은 polygalacturonase만이 7종 모두에서 monozyme이었고, 나머지 효소는 종마다 약간씩 다르게 나타났다. Isozyme banding patterns을 바탕으로 genetic similarity를 산출하여 dendrogram으로 보았을 때, 동일한 section Liseola에 속하는 F. subglutinans와 F. moniliforme의 종간 유사도는 74.3%로 가장 높았으며, section Elegans와 section Liseola의 서로 다른 section 간의 유사도는 45.4%로 비교적 낮았다. F. napiforme와 F. nygamai의 유사도는 64.7%로 동일 section 내의 종간 유사도와 비슷한 수치를 나타내었다. 이들 두 종은 section Liseola에 대하여 55.2%, section Elegans에 대하여 45.4%의 유사도를 보여 분류학적 위치에 있어서 section Liseola에 더 가까운 경향을 나타내지만, 이는 다른 section 간의 유사도와 비슷한 수치로 하나의 독립된 section으로 추정된다. F. graminearum과 다른 6종 사이의 유사도는 28.2%의 낮은 수치를 나타내어 서로의 유사성이 매우 낮다는 것을 보여 주었다.

• Journal of Microbiology
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• 제33권4호
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• pp.334-338
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• 1995

The electrophoretic karyotypes of 6 species in different Fusarium sections were examined by using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Intact chromosomal DNA was prepared from protoplasts and up to 9 distinct bands were separated on 0.7% or 0.8% agarose gel under several different conditions. Putative chromosome numbers varied from 6 to 9 amd polymorphic karyotypes were observed in different Fusarium sections. Using Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as standards, the sizes of the Fusarium spp. chromosomes were estimated. The electrophoretic karyotypes of F. moniliforme and F. subglutinans (section Liseola) were similar. Unidentified filamentour fungi, F. beomiforme was much closer to F. axysporum (section Elecgans) in karyotype and the karyotypes of F. napiforme were more similar to those of section Liseola than any other sections. F. graminearum (section Discolor) had a distinctive electrophoretic karyotype.