• The Korean Journal of Mycology
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• v.23 no.4 s.75
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• pp.359-368
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• 1995

F. napiforme, F. beomiforme and F. nygamai could not be classified in any of the existing sections of the genus Fusarium. To discuss of the exact taxonomic relationships among these species, the cellular polypeptide patterns were compared by using 2-D PAGE. Polypeptide pattern of F. beomiforme was different from those of other two species and was more similar to F. oxysporum in section Elegans. F. nygamai and F. napiforme might be another same section which would lie between section Liseola and section Elegans. The results were consistent with the comparison of isoenzyme patterns in these species.

• The Korean Journal of Mycology
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• v.18 no.3
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• pp.132-136
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• 1990

The vegetative nuclear divisions in hyphae and the chromosome of Fusarium were observed by use of HCI-Giemsa technique and light microscope. The chromosome of nuclear in F. moniliforme both 7150 and 7219 were eight. F. subglutinans 1082 was n=8 and n=7 in F. sub­glutinans 1083. F. nygamai 5668 was n=7 and n=5 in F. nygamai 7132. F. beomiforme 9758 and 9760 were n=7. F. coccidicola ATCC 24138 and F. acuminatum ATCC 16560 were n=6. From these results and other reports, the basic chromosomal number of these fungi might be speculated to be four.

• Journal of Microbiology
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• v.33 no.4
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• pp.334-338
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• 1995

The electrophoretic karyotypes of 6 species in different Fusarium sections were examined by using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Intact chromosomal DNA was prepared from protoplasts and up to 9 distinct bands were separated on 0.7% or 0.8% agarose gel under several different conditions. Putative chromosome numbers varied from 6 to 9 amd polymorphic karyotypes were observed in different Fusarium sections. Using Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as standards, the sizes of the Fusarium spp. chromosomes were estimated. The electrophoretic karyotypes of F. moniliforme and F. subglutinans (section Liseola) were similar. Unidentified filamentour fungi, F. beomiforme was much closer to F. axysporum (section Elecgans) in karyotype and the karyotypes of F. napiforme were more similar to those of section Liseola than any other sections. F. graminearum (section Discolor) had a distinctive electrophoretic karyotype.

• The Plant Pathology Journal
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• v.35 no.4
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• pp.301-312
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• 2019

Sorghum is the fifth most important cereal worldwide, spreading from Africa throughout the world. It is particularly important in the semi-arid tropics due to its drought tolerance, and when cultivated in Southeast Asia commonly occurs as a second crop during the dry season. We recovered Fusarium from sorghum in Thailand and found F. proliferatum, F. thapsinum and F. verticillioides most frequently, and intermittent isolates of F. sacchari and F. beomiforme. The relatively high frequencies of F. proliferatum and F. verticillioides, suggest mycotoxin contamination, particularly fumonisins and moniliformin, should be evaluated. Genetic variation within the three commonly recovered species was characterized with vegetative compatibility, mating type, Amplified Fragment Length Polymorphisms (AFLPs), and female fertility. Effective population number ($N_e$) was highest for F. verticillioides and lowest for F. thapsinum with values based on mating type allele frequencies higher than those based on female fertility. Based on AFLP genetic variation, the F. thapsinum populations were the most closely related, the F. verticillioides populations were the most distantly related, and the F. proliferatum populations were in an intermediate position. The genetic variation observed could result if F. thapsinum is introduced primarily with seed, while F. proliferatum and F. verticillioides could arrive with seed or be carried over from previous crops, e.g., rice or maize, which sorghum is following. Confirmation of species transmission patterns is needed to understand the agricultural systems in which sorghum is grown in Southeast Asia, which are quite different from the systems found in Africa, Australia, India and the Americas.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.386-393
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• 1994

To investigate taxonomical relationships of Fusarium species, $esterase-{\alpha},\;-{\beta}$, acid phosphatase, malate dehydrogenase, peroxidase and polygalacturonase were extracted and the isozyme patterns were compared by using polyacrylamide gel electrophoretic analysis. Only polygalacturonase was monozyme and the other enzymes showed little differences in banding patterns. Genetic similarities based on the isozyme banding patterns were as follows: the interspecific similarity between F. subglutinans and F. moniliforme in Liseola showed the closest relationship of 74.3% of all species studies. And the similarity between section Elegans and section Liseola was 45.4%. F. napiforme and F. nygamai showed the similarity of 64.7%, similar to the correlation between species in the same section. The similarity of these two species to Liseola and Elegans showed 55.2% and 45.4%, being revealed that they would be closer to Liseola than Elegans. However, these results were similar to those of any other sections. Therefore it suggested that these 2 species should be in a different section from any other sections. And F. graminearum showed the similarity of 28.2% to the other 6 species.