• Journal of Microbiology
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• 제43권4호
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• pp.331-336
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• 2005

The objective of this study was to assess the strain-specificity of a DNA probe, Fu12, for Fusobacterium nucleatum subsp. nucleatum ATCC $25586^T$ (F. nucleatum ATCC $25586^T$), and to develop sets of strain-specific polymerase chain reaction (PCR) primers. Strain-specificity was tested against 16 strains of F. nucleatum and 3 strains of distinct Fusobacterium species. Southern blot hybridization revealed that the Fu12 reacted exclusively with the HindIII-digested genomic DNA of F. nucleatum ATCC $25586^T$. The results of PCR revealed that three pairs of PCR primers, based on the nucleotide sequence of Fu12, generated the strain-specific amplicons from F. nucleatum ATCC $25586^T$. These results suggest that the DNA probe Fu12 and the three pairs of PCR primers could be useful in the identification of F. nucleatum ATCC $25586^T$, especially with regard to the determination of the authenticity of the strain.

• 치위생과학회지
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• 제6권4호
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• pp.231-235
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• 2006

본 연구는 부가중합형실리콘인상재의 정밀 인상채득에 영향을 미치는 요인, 압축변형률, 탄성회복률, 점주도와 젖음성을 평가하기 위하여 현재 시판되고 있는 4종류의 인상재 Examixfine(GC), Aquasilultra(Dentsply), Extrude(Kerr), Perfect-F(Handae)를 이용하여 실험하였다. 실험 데이터는 SPSSWIN 12.0을 사용하여 분석하였고, 결과는 다음과 같다. 1. 압축변형률은 Extrude가 4.86%로 가장 낮게 나타났고, Examixfine이 6.78%로 가장 크게 나타났다. Examixfine과 Perfect-F 사이에는 유의한 값의 차이가 없었으나, 다른 인상재 그룹에서는 유의성이 있었다. (p < 0.001). 2. Extrude의 탄성회복률이 99.83%로 가장 크게 나타났고, Perfect-F가 96.54%로 가장 낮게 나타났다. Examixfine과 Aquasilultra 간에는 유의성이 없었으나, 다른 인상재그룹간에는 유의한 차이가 있었다(p < 0.001). 3. 점주도의 측정결과에서 Examixfine이 점주도 값이 45.12mm로 가장 높았고, Perfect-F의 경우 40.28 mm로 가장 낮은 점주도 값을 나타내었다. Examixfine과 Aquasilultra, Extrude와 Perfect-F 간에는 유의한 차이가 나타나지 않았다(p > 0.001). 4. 젖음성에서는 Aquasilultra가 $47.85^{\circ}$로 가장 접촉각이 작게 나타나 친수성이 가장 우수하게 나타났고, Perfect-F는 접촉각이 $94.89^{\circ}$로 접촉각이 크게 나타나 소수성으로 나타났다. 인상재 종류별로도 각각 유의성이 존재하였다 (p < 0.001). 5. 접촉각은 압축변형률(p < 0.01), 탄성회복률(p < 0.01), 점주도(p < 0.05)와 상관관계가 있었고, 탄성회복률과 점주도 와는 음의 상관관계를 보였다. 압축변형률은 접촉각, 탄성회복률과 상관관계가 있었고(p < 0.01), 탄성회복률과 음의 상관관계를 나타냈다. 탄성회복률은 접촉각, 압축변형률과 음의 상관관계를 가지고 있었고(p < 0.01), 점주도도 접촉각과 음의 상관관계를 보였다(p < 0.05).

• Journal of Microbiology and Biotechnology
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• 제10권6호
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• pp.789-796
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• 2000

In order to analyze the effects of tktA, $aroF^{FBR}$, and aroL expression in a tryptophan-producing Escherichia coli, a series of plasmids carrying the genes were constructed. Introduction of tktA, $aroF^{FBR}$, and aroL into the E. coli strain resulted in approximately 10-20 fold increase in the activities of transketolase, the feedback inhibition-resistant 3-deoxy-D-arabinoheptulsonate-7-phosphate synthase, and shikimate kinase. Expression of $aroF^{FBR}$ in the aroB mutant strain of E. coli resulted in the accumulation of 10 mM of 3-deoxy-D-arabinoheptulsonate-7-phosphate (DAHP) in the medium. Simultaneous expression of tktA and $aroF^{FBR}$ in the strain further increased the amount of excreted DAHP to 20 mM. In contrast, the mutant strain which has no gene introduced accumulated 0.5 mM of DAHP. However, the expression of tktA and $aroF^{FBR}$ in a tryptophan-producing E. coli strain did not lead to the increased production of tryptophan, but instead, a significant amount of shikimate, which is an intermediate in the tryptophan biosynthetic pathway, was excreted to the growth medium. Despite the fact that additional expression of shikimate kinase in the strain could possibly remove 90% of excreted shikimate to 0.1 mM, the amount of tryptophan produced was still unchanged. Removing shikimate using a cloned aroL gene caused the excretion of glutamate, which suggests disturbed central carbon metabolism. However, when cultivated in a complex medium, the strain expressing tktA, $aroF^{FBR}$, and aroL produced more tryptophan than the parental strain. These data indicate that additional rate-limiting steps are present in the tryptophan biosynthetic pathway, and the carbon flow to the terminal pathway is strictly regulated. Expressing tktA in E. coli cells appeared to impose a great metabolic burden to the cells as evidenced by retarded cell growth in the defined medium. Recombinant E. coli strains harboring plasmids which carry the tktA gene showed a tendency to segregate their plasmids almost completely within 24h.

• 한국미생물·생명공학회지
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• 제23권5호
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• pp.617-623
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• 1995

A new thermotolerant yeast strain was siolated, and its characteristics have been studied. The strain was identified and named Saccharomyces cerevisiae F38-1. This strain could grow not only at high temperature, but also in high concentrations of sugar and ethanol. S. cerevisiae F38-1 could grow in a medium containing 50% glucose. The isolate produced ethanol at 43$\circ$C, but didn't grow at 40$\circ$C in the presence of 8% ethanol. Fermentation studies showed that the isolate ferments 20% glucose to 9.8% (V/V) ethanol at 40$\circ$C in the presence of 0.2%, yeast extract.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1490-1494
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• 2014

To probe the genomic properties of microbes thriving in desert lakes, we sequenced the full genome of a betaproteobacterial strain (SL110) belonging to the understudied genus Caenimonas of the family Comamonadaceae. This strain was isolated from a freshwater lake in the western Gobi Desert, Northern China. Its genome contains genes encoding carbon monoxide dehydrogenase, nitrate reductase, nitrite reductase, nitric oxide reductase, and sulfur oxidation enzymes, highlighting the potentially important contribution of this group of bacteria to the cycling of inorganic elements in nature. Unexpectedly, a coenzyme $F_{420}$ biosynthesis gene cluster was identified. A further search for $F_{420}$ biosynthesis gene homologs in genomic databases suggests the possible widespread presence of $F_{420}$ biosynthesis gene clusters in proteobacterial genomes.

• Mycobiology
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• 제48권1호
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• pp.37-43
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• 2020

A serious leaf disease caused by Colletotrichum dematium was found during the cultivation of Sarcandra glabra in Jingxi, Rong'an, and Donglan Counties in Guangxi Province, which inflicted huge losses to plant productivity. Biological control gradually became an effective control method for plant pathogens. Many studies showed that the application of actinomycetes in biological control has been effective. Therefore, it may be of great significance to study the application of actinomycetes on controlling the diseases caused by S. glabra. Strains of antifungal actinomycetes capable of inhibiting C. dematium were identified, isolated and screened from healthy plants tissues and the rhizospheres in soils containing S. glabra. In this study, 15 actinomycetes strains were isolated and among these, strains JT-2F, DT-3F, and JJ-3F, appeared to show antagonistic effects against anthracnose of S. glabra. The strains JT-2F and DT-3F were isolated from soil, while JJ-3F was isolated from plant stems. The antagonism rate of strain JT-2F was 86.75%, which was the highest value among the three strains. Additionally, the JT-2F strain also had the strongest antagonistic activity when the antagonistic activities were tested against seven plant pathogens. Strain JT-2F is able to produce proteases and cellulase to degrade the protein and cellulose components of cell walls of C. dematium, respectively. This results in mycelia damage which leads to inhibition of the growth of C. dematium. Strain JT-2F was identified as Streptomyces tsukiyonensis based on morphological traits and 16S rDNA sequence analysis.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집C
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• pp.586-591
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• 2001

In the cold forging, elastic deformation of the die has been investigated to improve the accuracy of cold forged parts with F.E.M analysis using DEFORM, and with experiments using strain gauges. In the experiments, initial billet was selected to easily find the effect of elastic deformation according to the forging modes, extrusion and upsetting type, and only extrusion type. Elastic deformation of the die can be obtained by the signal from the strain gauges and this signal can be amplified by data acquisition system during the process. In the F.E.M analysis, two types of analysis are used to predict elastic strain of the die. To improve an accuracy of forged product and die dimension, this study compared with strain distribution between experiment and F.E.M analysis. As a result, the history of the deformation of the die and elastic recovery of forged product can be obtained by the elastic strain analysis of forged product and die according to the forging modes.

• 한국응용곤충학회지
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• 제11권2호
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• pp.85-89
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• 1972

1. 전국에서 채집 분리한 51개의 균주를 phage의 용 균형에 따라 분류하여 8가지 계통으로 나누었다. 2. 8가지 계통 중 F-계통이 우리나라에 가장 많았으며 H.C.A계통이 그 다음 순이었고 일본이나 남방계에 많은 D-계통은 발견되지 않았다. 3. 우리나라에 분포하고 있는 흰빛잎마름병균은 지역에 따라서 계통이 한정되거나 특이성을 보이지 않았다. 4. 통일품종은 단일품종내에서 6가지의 계통이 분리되었으나 다른 품종에서는 3가지 이내의 계통이 분리되었다.

• Nuclear Engineering and Technology
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• 제43권1호
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• pp.83-88
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• 2011

To define the effect of strain rate variation from 0.04% to 0.004%/s on environmental fatigue of CF8M cast stainless steel, which is used as a primary piping material in nuclear power plants, low-cycle fatigue tests were conducted at operating pressure and temperature condition of a pressurized water reactor, 15 MPa and $315^{\circ}C$, respectively. A high-pressure and high-temperature autoclave and cylindrical solid fatigue specimens were used for the strain-controlled low-cycle environmental fatigue tests. It was observed that the fatigue life of CF8M stainless steel is shortened as the strain rate decreases. Due to the effect of test temperature, the fatigue data of NUREG-6909 appears a slightly shorter than that obtained by KEPRI at the same stress amplitude of $1{\times}10^3$ MPa. The environmental fatigue correction factor $F_{en}$'s calculated with inputs of the test data increases with high strain amplitude, while the $F_{en}$'s of NUREG-6909 remain constant regardless of strain amplitude.

• 대한건축학회논문집:구조계
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• 제35권3호
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• pp.27-33
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• 2019

The purpose of this study is to provide and evaluate the tensile properties of masonry element such as tensile strength, strain, modulus of elasticity and stress-strain relationship through the direct tension test with varies of mortar strength. From the experiment, the tension fracture was observed along the interfaces between the brick and the mortar. Tension properties of masonry element was significantly affected by compressive strength of mortar, $f_m$, indicating that higher tensile strength and modulus of elasticity of masonry element were obtained with increase of $f_m$. The strain of a masonry element was inversely proportional to $f_m$ due to the lower ductility of a higher mortar strength. A tensile stress-strain relationship of masonry element was generalized based on the numerical analysis and the regression analysis using test data. The proposed model shows fairly good agreement with the test measurements.