• Title/Summary/Keyword: F strain

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Genetical Aspects of the Feeding Habit in Fb Strain, Bombyx mori. (식성이상잠 Fb잠의 섭식서엥 관한 유전학적 분석)

  • 김경아;노시갑
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.113-117
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    • 1996
  • To analyze the mode of inheritance of this gene in Fb strain, carried out cross experiment between Fb and several Japanese and Chinese origin strains. The results of crossing experiments, feeding ratio in F1 hybrids were nearly the mid of parents, F2 and backcrossed hybrids(BF1) were approximately intermediate between Fb and other strains. Hence, the feeding habit of cabbage leaves on Fb strain is assume to be incomplete dominance.

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Dynamic Stress/Strain Measurement and Analysis of the Aluminum Alloy Road Wheel through F1 Circuit Ultimate Driving Test (F1 서킷 극한주행시험을 통한 알루미늄 알로이 휠의 동응력/변형률 계측 및 분석)

  • Lee, Chang Soo;Park, Cheol Soon;Park, Hyung Bae;Jung, Sung Pil;Chung, Won Sun
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2014.10a
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    • pp.612-617
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    • 2014
  • It is generally known that the automotive road wheel involves the non-proportional multiaxial loading condition, therefore the measuring dynamic stress and strain in driving state is very important to predict an endurance characteristic of the automotive road wheel. In this study, the ultimate driving test using F1 circuit with respect to 2 kinds of velocity conditions have been carried out in order to measure dynamic stress, strain of the wheel and acceleration of a vehicle. Based on the measured results, the characteristics of dynamic stress generation have been analyzed, and factors which have effect on the dynamic stress generation have been studied.

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Structure and Function of the phnF Gene of Pseudomonas sp. Strain DJ77 (Pseudomonas sp. Strain DJ77에서 phnF 유전자의 구조)

  • 이성훈;김성재;신명수;김치경;임재윤;이기성;민경희;김영창
    • Korean Journal of Microbiology
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    • v.33 no.2
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    • pp.92-96
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    • 1997
  • The 6.8 kb Xhol fragment of chromosomal ONA of Pseudomonas sp. 0177 contains the phnDEFG genes involved in the degradation of polyaromatic hydrocarbons and chlorinated aromatics. Here, we report the nucleotide sequence of the ORF encoding a polypeptide consisted of 143 amino acids with a Mr of 13,859. The nucleotide sequence of the ORF is 99% and 68.6% identical to the downstream region of catE of Sphingomonas sp. strain HV3 and the ORF between xylE and xylG of Sphingomonas yanoikuyae Bl, respectively. The deduced amino acid sequence of the PhnF has 62.3% identity with the amino acid encoded hy orfY region of Citrobacter freundii DSM30040. We now confirm that the ORF is located between the catechol 2,3-dioxygenase (C230), phnE, and 2-hydroxymuconic semialdehyde dehydrogenase (2HMSO), phnG.

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Isolation, Identification and Characterization of Bacteria Degrading Crude Oil (원유 분해 미생물의 분리, 동정 및 특성)

  • Oh, Kyoung-Taek;Lee, Yong-Woon;Kubo, Motoki;Kim, Seong-Jun;Chung, Seon-Yong
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.10
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    • pp.1851-1859
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    • 2000
  • Crude oil-degrading bacteria were isolated from the sites contaminated by oil products. The isolates were identified as Acinetobacter sp. A132, Pseudomonas putida A422, Pseudomonas aeruginosa F721, F722, and Xanthomonas maltophilia B823. The results of investigation on the degradability of crude oil indicated that the strain A132 had the highest rate of $6.04g/L{\cdot}day$. Also, the strain A132 and F722 almost degraded each of n-alkane compounds between $nC_{10}$ and $nC_{32}$. The strain A422 degraded benzene and xylene but not n-alkane. The strain B823 grew somewhat in crude oil but did not entirely degrade other substrates used in this study. The results of the GC/FID analysis on the degradability of the mixed n-alkane compounds showed that the strain F722 could degrade 100% of the compounds with $nC_7{\sim}nC_{10}$ and more than 80% of those with $nC_{11}{\sim}nC_{24}$.

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Evaluation for Applications of Displacement Criterion by the Critical Strain of Uniaxial Compression in Rock Mass Tunnel (일축압축 한계변형률에 의한 암반터널 변위기준 적용성 평가)

  • Kim, Young-Su;Kim, Dae-Man
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.6C
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    • pp.321-329
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    • 2009
  • Laboratory compressive test was conducted on 6 different types of rock in order to investigate the characteristic of critical strain under uniaxial and triaxial stress condition. The results of uniaxial compressive test mostly ranged within 1~100MPa, the critical strain was also located between 0.1~1.0%. Therefore the results distributed within the upper and lower boundary proposed by Sakurai (1982). And the failure/critical strain ratio (${\varepsilon}_f/{\varepsilon}_0$) showed between 1.0~1.8 value depending on the uniaxial compressive strength. The results of critical strain by triaxial compressive test showed below 0.8% value for all test, the M value calculated from uniaxial and triaxial compressive test results ranged 1.0~8.0 for most of rock specimens. It is concluded that failure strain (${\varepsilon}_{f3}$) of rock mass, which is in triaxial stress condition is larger than the results of uniaxial stress condition (${\varepsilon}_{f1}$) by 1.0~8.0 times and value showed 1.0~1.8 larger value than critical strain (${\varepsilon}_{01}$). Therefore it is a conservative way for rock tunnel to use critical strain (${\varepsilon}_{01}$) calculated from a uniaxial compressive strength on tunnel displacement monitoring.

Genome Shuffling of Mangrove Endophytic Aspergillus luchuensis MERV10 for Improving the Cholesterol-Lowering Agent Lovastatin under Solid State Fermentation

  • El-Gendy, Mervat Morsy Abbas Ahmed;Al-Zahrani, Hind A.A.;El-Bondkly, Ahmed Mohamed Ahmed
    • Mycobiology
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    • v.44 no.3
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    • pp.171-179
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    • 2016
  • In the screening of marine mangrove derived fungi for lovastatin productivity, endophytic Aspergillus luchuensis MERV10 exhibited the highest lovastatin productivity (9.5 mg/gds) in solid state fermentation (SSF) using rice bran. Aspergillus luchuensis MERV10 was used as the parental strain in which to induce genetic variabilities after application of different mixtures as well as doses of mutagens followed by three successive rounds of genome shuffling. Four potent mutants, UN6, UN28, NE11, and NE23, with lovastatin productivity equal to 2.0-, 2.11-, 1.95-, and 2.11-fold higher than the parental strain, respectively, were applied for three rounds of genome shuffling as the initial mutants. Four hereditarily stable recombinants (F3/3, F3/7, F3/9, and F3/13) were obtained with lovastatin productivity equal to 50.8, 57.0, 49.7, and 51.0 mg/gds, respectively. Recombinant strain F3/7 yielded 57.0 mg/gds of lovastatin, which is 6-fold and 2.85-fold higher, respectively, than the initial parental strain and the highest mutants UN28 and NE23. It was therefore selected for the optimization of lovastatin production through improvement of SSF parameters. Lovastatin productivity was increased 32-fold through strain improvement methods, including mutations and three successive rounds of genome shuffling followed by optimizing SSF factors.

Discrete element numerical simulation of dynamic strength characteristics of expanded polystyrene particles in lightweight soil

  • Wei Zhou;Tian-shun Hou;Yan Yang;Yu-xin Niu;Ya-sheng Luo;Cheng Yang
    • Geomechanics and Engineering
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    • v.34 no.5
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    • pp.577-595
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    • 2023
  • A dynamic triaxial discrete element numerical model of lightweight soil was established using the discrete element method to study the microscopic mechanism of expanded polystyrene (EPS) particles in the soil under cyclic loading. The microscopic parameters of the discrete element model of the lightweight soil were calibrated depending on the dynamic triaxial test hysteresis curves. Based on the calibration results, the effects of the EPS particles volume ratio and amplitude on the contact force, displacement field, and velocity field of the lightweight soil under different accumulated strains were studied. The results showed that the hysteresis curves of lightweight soil exhibit nonlinearity, hysteresis, and strain accumulation. The strain accumulated in remolded soil is mainly tensile strain, and that in lightweight soil is mainly compressive strain. As the volume ratio of EPS particles increased, the contact force first increased and then decreased, and the displacement and velocity of the particles increased accordingly. With an increase in amplitude, the dynamic stress of the particle system increased, and the accumulation rate of the dynamic strain of the samples also increased. At 5% compressive strain, the contact force of the particles changed significantly and the number of particles deflected in the direction of velocity also increased considerably. These results indicated that the cemented structure of the lightweight soil began to fail at a compressive strain of 5%. Thus, a compressive strain of 5% is more reasonable than the dynamic strength failure standard of lightweight soil.

A Study on Analyzing Thermal Strain of Weldment during Cooling used at Low MS Point Weld Consumables (MS Point 저감 용접재에 적용한 냉각시 용접부 열변형률 분석에 관한 연구)

  • Ha, Yunsok;Nam, Seongkil;Park, Sejin;Kwon, Changgil
    • Journal of Welding and Joining
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    • v.31 no.6
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    • pp.37-43
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    • 2013
  • This study targets to make clear the connection between MS (Martensite start) point and welding shrinkage. We approved that a Martensite-transformed weldment may not yield state under low MS point, but also admitted the limitation of numerical calculation by inherent strain approach or thermal strain approach. Therefore, new thermal strain formulae during cooling stages were made. As a thermal strain is obtained by integrating thermal extension coefficient, a constant of integration should be decided. In our suggested formulae, the origin was based on totally remained austenite, and added strain from volume changes in Martensite transformation was based on totally transformed ferrite. Through the suggested methodology, It is verified that an MS point under a critical temperature can let weld shrinkage relax and the critical value can be obtained. For supporting this process, 15 weld-consumables were made, were tested by fillet type and were measured. As a result, a positive correlation between MS point and level of weld-distortion was obtained, but it was rather weak.

Isolation and Identification of an Unreported Fungal Species in Korea and Novel Ice Nucleation Active Fungus: Fusarium diversisporum

  • Diane Avalos-Ruiz;Gwang-Jae Lim;Seong-Keun Lim;Leonid N. Ten;In-Kyu Kang;Seung-Yeol Lee;Hee-Young Jung
    • The Korean Journal of Mycology
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    • v.50 no.4
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    • pp.255-262
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    • 2022
  • In this study, the fungal strain KNUF-21-F39 was isolated from a declined apple tree (Malus domestica) in the Chungcheongbuk province in Korea. The strain KNUF-21-F39 presented a slow growth rate and a variety of macroconidia shapes and sizes ranging from ovoid to fusoid and 1- to 5-septate, primarily showing 3- and 4-septate, with "S" -shaped macroconidia rarely observed. The strain was identified based on morphological characteristics along with phylogenetic analysis performed using the internal transcribed spacer region (ITS) and partial sequences of translation elongation factor 1-α (tef1), RNA polymerase largest subunit (rpb1), and calmodulin (cal) genes. The fungal strain KNUF-21-F39 was identified as Fusarium diversisporum, which has not been previously reported in Korea. The ice nucleation activity (INA) of the strain was also evaluated, identifying the strain as positive for INA. This is the first report characterizing F. diversisporum as an IN-active fungal species.

Effects of Mg and Cu Additions on Superplastic Behavior in MA Aluminum Alloys

  • Han, Chang-Suk;Jin, Sung-Yooun;Bang, Hyo-In
    • Korean Journal of Materials Research
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    • v.28 no.8
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    • pp.435-439
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    • 2018
  • MA Al alloys are examined to determine the effects of alloying of Mg and Cu and rolling on tensile deformation behavior at 748 K over a wide strain rate range($10^{-4}-10^3/s$). A powder metallurgy aluminum alloy produced from mechanically alloyed pure Al powder exhibits only a small elongation-to-failure(${\varepsilon}_f$ < ~50%) in high temperature(748 K) tensile deformation at high strain rates(${\acute{\varepsilon}}=1-10^2/s$). ${\varepsilon}_f$ in MA Al-0.5~4.0Mg alloys increases slightly with Mg content(${\varepsilon}_f={\sim}140%$ at 4 mass%). Combined addition of Mg and Cu(MA Al-1.5%Mg-4.0%Cu) is very effective for the occurrence of superplasticity(${\varepsilon}_f$ > 500%). Warm-rolling(at 393-492 K) tends to raise ${\varepsilon}_f$. Lowering the rolling-temperature is effective for increasing the ductility. The effect is rather weak in MA pure Al and MA Al-Mg alloys, but much larger in the MA Al-1.5%Mg-4.0%Cu alloy. Additions of Mg and Cu and warm-rolling of the alloy cause a remarkable reduction in the logarithm of the peak flow stress at low strain rates (${\acute{\varepsilon}}$< ~1/s) and sharpening of microstructure and smoothening of grain boundaries. Additions of Mg and Cu make the strain rate sensitivity(the m value) larger at high strain rates, and the warm-rolling may make the grain boundary sliding easier with less cavitation. Grain boundary facets are observed on the fracture surface when ${\varepsilon}_f$ is large, indicating the operation of grain boundary sliding to a large extent during superplastic deformation.