• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.181-186
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• 2019

The optimum design of LED device for irradiation of 460 nm blue light on extruded rice cake using simulation and the effect of the blue light on the inactivation of Bacillus cereus (B. cereus) group on the rice cake were investigated. The irradiated light intensity patterns on the surface area of the sample were simulated with three different LED arrays (centered, cross, and evenly spaced) and at various distances (22, 32, 42 mm) between the LED modules and the sample. In addition, the uniformity was calculated as Petri factor. The evenly spaced array resulted the most uniform light intensity pattern in the simulation, and the Petri factor of 32 and 42 mm of the distances showed higher than 0.9, which represents the ideal uniformity of LED device. The bacterial population of the rice cake decreased to less than the initial bacterial population during exposure to LED blue light, whereas the bacterial population of the control sample increased. The bacterial count of the rice cake after blue light irradiation for 24 h was 1.21 log CFU/g lower than the control sample. Petri factor increased with increase of the distance between the light source and sample, however, the reduction rate of B. cereus group decreased. Therefore, the design of LED device, that represented the Petri factor higher than 0.9 and inactivated the population of B. cereus group, with evenly spaced and 32 mm of distance between the light source and sample was suitable for extending shelf-life of rice cake.