• 한방안이비인후피부과학회지
• /
• 제23권2호
• /
• pp.69-80
• /
• 2010

Objective : The purpose of this study was to compare anti-Inflammation and anti-oxidation of Jeondo-San(JDS) extracted with two kinds of solvents, ethanol and water. Methods : Two kinds of JDS extractions were prepared 20, 50, $100\;{\mu}g/mg$. The Cytotoxicity was measured by MTT assay in Raw 264.7 cell. The anti-inflammation effects were measured by inhibitory efficacy on $PGE_2$, NO, TNF-$\alpha$, COX-2 and iNOS in Raw 264.7 cell. The anti-oxidation effects were measured by ROS inhibitory efficacy, intracellular GSH synthesis and DPPH Radical scavenging in HaCaT cell. Results : 1. All of JDS extraction groups had no cytotoxicity in Raw 264.7 cell. 2. All of JDS extraction groups showed significantly inhibitory effect on production of $PGE_2$. Inhibitory efficacy increased in accodance with concentration. 3. All of JDS extraction groups showed significantly inhibitory effect on production of NO. Inhibitory efficacy increased in accodance with concentration. 4. All of JDS extraction groups did not show significantly inhibitory effect on production of TNF-$\alpha$. 5. $100\;{\mu}g/ml$ JDS extracted with ethanol and $50\;{\mu}g/ml$, $100\;{\mu}g/ml$ JDS extracted with water showed inhibitory effect on iNOS expression. 6. All of JDS extraction groups showed significantly inhibitory effect on production of ROS. Inhibitory efficacy increased in accodance with concentration. Ethanol extractions were better than water extractions. 7. $100\;{\mu}g/ml$ JDS extracted with ethanol only produced GSH of $32{\pm}5.2%$. 8. All of JDS extraction groups showed significantly scavenging effect of DPPH radicals. Inhibitory efficacy increased in accodance with concentration. Ethanol extractions were better than water extractions. Conclusion : Two kinds of JDS extractions have not cytotoxicity and inhibit production of NO. JDS extracted with water was effective in anti-inflammation, JDS extracted with ethanol was effective in anti-oxidation.

• 대한약침학회지
• /
• 제14권1호
• /
• pp.79-86
• /
• 2011

Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Cuscutae Semen(CS) extracted with two kinds of solvents, ethanol and distilled water. Methods : Two kinds of CS extractions were prepared 20, 50, 100 ${\mu}l/mg$. The cytotoxicity was measured by MTT assay in Raw 264.7 cell. The anti-inflammation effect was measured by inhibitory efficacy of NO Production in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. Two kinds(100 ${\mu}l/mg$) of CS extraction groups had 50% cytotoxicity in Raw 264.7 cell. 2. All of CS extraction groups were not showed significantly inhibitory effect on NO production. 3. All of CS extracted with ethanol only showed dose-dependently significantly scavenging effect of DPPH radicals. 4. Two kinds of CS extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds(100 ${\mu}l/mg$) of CS extraction groups have 50% cytotoxicity. Two kinds of CS extractions have not the inhibitory effect on NO production and Propionibactrium acnes. CS groups extracted with ethanol only have a significantly scavenging ability of DPPH radicals. This study suggests that CS extracted with ethanol was effective in anti-oxidation.

• 대한약침학회지
• /
• 제14권1호
• /
• pp.71-78
• /
• 2011

Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Ligustici Rhizoma (LR) extracted with two kinds of solvents, ethanol and distilled water. Methods : It is prepared two kinds of LR extracts 20, 50, 100 ${\mu}l/mg$ by first. MTT assay way to measure cytotoxicity is formed in Raw 264.7 cell. The anti-inflammation effect is measured by ability to inhibit production of NO in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. LR (20 ${\mu}l/mg$) extracted with ethanol was showed 80% cytotoxicity, LR (50 ${\mu}l/mg$) extracted with ethanol and LR (20, 50 ${\mu}l/mg$) extracted with water were showed 70% cytotoxicity, LR (100 ${\mu}l/mg$) extracted with ethanol and LR (100 ${\mu}l/mg$) extracted with water were showed 60% cytotoxicity in Raw 264.7 cell. 2. LR (100 ${\mu}l/mg$) extracted with ethanol was showed more significantly inhibitory effect on NO production than the water extraction. 3. Two kinds of LR extraction groups did not show significantly scavenging effect of DPPH radicals. 4. Two kinds of LR extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds of LR extracts have not cytotoxicity, statistically significant ability to scavenge DPPH radicals and effect to inhibit Propionibactrium acnes. LR extracted with ethanol only have a little effect to inhibit NO production. This study proposes that LR extracted with ethanol is more effective in anti-inflammation.

• 공업화학
• /
• 제7권1호
• /
• pp.162-170
• /
• 1996

접촉분해경유(Light Cycle Oil : LCO)중에는 엔지니어링 플라스틱 및 고분자 액정 등의 기초 원료로서 각광 받고 있는 2,6-dimethylnaphthalene(2,6-DMNA)이 함유되어 있다. 본 연구는, LCO중에 함유된 2,6-DMNA의 분리, 정제를 위한 전처리조작으로서 LCO중에 함유된 dimethylnaphthalene(DMNA)이성체 혼합물의 농축을 추출-증류의 조합에 의해 실험적으로 검토했다. 또, LCO중에 함유된 DMNA이성체 혼합물의 농축에 대한 증류와 추출-증류의 조합을 비교, 검토하였다. Dimethylsulfoxide수용액을 용매로 사용한 회분병류 5회 평형추출로서 LCO중에 함유된 방향족 성분을 회수한 결과, 회수된 추출유중의 나프탈렌류 성분(탄소수 : 10-12)의 농도는 LCO중의 나프탈렌류 성분의 그것에 비해 높았으며, 나프탈렌류 성분의 수율은 탄소수가 작은 성분일수록 컸다. 5회 평형추출에 의한 DMNA이성체 혼합물의 회수율은 65wt%이었다. 추출유의 회분증류로서 얻어진 DMNA을 함유한 유출액을 재차 회분증류함으로서 60wt%의 고농도 DMNA이성체 혼합물을 함유한 유출액의 회수가 가능했다. LCO중에 함유된 DMNA이성체 혼합물의 농축에는 LCO의 직접증류보다 추출-증류의 조합이 더 효과적이었다.

• Current Research on Agriculture and Life Sciences
• /
• 제29권
• /
• pp.83-89
• /
• 2011

쇠비름내의 여러 가지 기능성 유용성분을 효율적으로 추출하기 위해서, 반응표면분석법(RSM)을 이용하여 유용 성분 용매 추출 조건을 최적화하였다. 쇠비름을 에탄올에 추출하여 반응표면 분석법으로 모니터링하여 최적 용매 조건을 설정하였다. 중심합성계획법에 따라 에탄올 농도 ($X_1$), 추출 온도 ($X_2$), 추출 시간 ($X_3$)를 요인변수로 설정하고 총 페놀 함량($Y_1$), 총 플라보노이드 함량($Y_2$), 전자공여능($Y_3$), 갈색도($Y_4$)을 종속 변수로 하였다. 쇠비름의 추출 조건에 따라 총 페놀 함량 측정결과, 총 페놀함량은 추출온도와 추출시간에 영향을 덜 받았고 에탄올 농도에는 영향을 받았고, 에탄올 농도는 46.23%, 추출온도는 $92.36^{\circ}C$, 추출시간은 2.49hr에서 최대값인 32.17mg/mL로 나타났다. 각각의 추출조건에 따른 추출물들의 총 플라 보노이드 함량 변화는 추출시간에 따라 영향을 받았으며, 에탄올 농도는 96.62%, 추출온도 $61.87^{\circ}C$, 추출시간 3.70hr에서 최대값이 58.28mg/mL로 나타났다. 쇠비름 추출물의 최적 추출조건을 contour map을 superimposing하여 예측한 결과, 최적 추출조건은 에탄올농도 70.3%, 추출온도 $62.1^{\circ}C$, 추출시간 3.3hr로 예측되었다. 각 추출조건에 따른 전자공여능(EDA)을 측정한 결과 에탄올 농도에 가장 영향을 많이 받았고, 에탄올 농도 52.95%, 추출온도 $52.33^{\circ}C$, 추출시간 4.64hr에서 최대값 74.67mg/mL로 나타났다. 각 추출조건에 따라 갈색도(browning color)를 측정한 결과, 갈색도는 추출시간에는 거의 영향을 받지 않았고, 에탄올 농도와 추출온도에 영향을 받았다. 에탄올 농도 97.75%, 추출온도는 $65.88^{\circ}C$, 추출시간은 2.93hr에서 최대값이 0.80 OD로 나타났다.

• 한국식품과학회지
• /
• 제15권2호
• /
• pp.141-149
• /
• 1983

본(本) 실험(實驗)은 말쥐치 단백질(蛋白質) 조제품(調製品)의 생산(生産)을 위한 최적조건을 조사하고, 단백질(蛋白質) 제품(製品)의 식품학적 성질을 알고자 하였다. 말쥐치 농축(濃縮) 단백질(蛋白質)과 말쥐치 분리(分離) 단백질(蛋白質)을 여러 유기용매(有機溶媒)를 사용(使用)하여 내장과 머리를 제거(除去)하거나 또는 전어(全魚)로 한 말쥐치를 추출(抽出)에 의하여 조제(調製)하였다. 그 결과는 다음과 같았다. 1. 용매(溶媒)로 사용(使用)한 iso-propyl alcohol은 지방(脂肪)의 추출(抽出) 및 어육(魚肉)의 trimethyl-amine의 추출(抽出)에 매우 효과적(效果的)이었다. 2. 최적추출시간(最適抽出時間)은 교반(30rpm)하면서 $65{\sim}70^{\circ}C$에서 iso-propyl alcohol이나 ethyl alcohol로 20분(分)이었다. 3. 원료(原料) 말쥐치의 중량(重量)에 대한 가장 효과적(效果的)인 유기용매(有機溶媒)의 비율은 1차(次) 추출에서 5 : 1이었고, 2차(次) 추출은 2 : 1이 적당하였다. 4. 단배질(蛋白質) 조제품(調製品)의 지방함량(脂肪含量)은 머리와 내장을 제거(除去)한 말쥐치에는 3회(回)까지 추출(抽出)한 후 잔류 지방량을 0.5% 이하로 감소할 수 있었고, 전어(全魚)의 경우에는 5회(回) 추출(抽出) 후(後) $0.27{\sim}0.31%$의 지방함량(脂肪含量)을 나타냈다. 5. 농축(濃縮) 단백질(蛋白質)과 분리(分離) 단백질(蛋白質)의 단백질(蛋白質) 함량(含量)은 81.08%와 87.41%이었고, 두 단백질(蛋白質) 조제품(調製品)의 지방함량(脂肪含量)은 각각 0.43%와 0.45%이었다. 6. Ca함량(含量)은 분리(分離) 단백질(蛋白質)보다는 농축(濃縮) 단백질(蛋白質)이 높은 것으로 나타났다. 분리(分離) 단백질(蛋白質)에서의 Na와 K의 성분(成分)은 검출되지 않은 반면 생선 농축물에서는 상당한 양(量)을 함유(含有)하고 있는 것으로 나타났다. 7. 말쥐치 분리(分離) 단백질(蛋白質)에서의 suspended solids, wettability, emulsion stability, foam viscosity와 같은 식품학적 성질은 말쥐치 농축(濃縮) 단백질(蛋白質)보다 더 높은 경향을 나타냈다.