• The Korean Journal of Physiology
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• v.25 no.2
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• pp.115-123
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• 1991

Molecular association of glucose transporters with the other proteins in the plasma membrane was assessed by gel electrophoresis and immunoblot techniques. Approximately $31.5{\pm}5.1%$ of GLUT-4, $64.8{\pm}2.7%$ of clathrin, 48.7% of total protein in the plasma membrane (PM) were found insoluble upon extraction with 1% Tx-100. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the Tx-100 insoluble PM fraction contained about 4 major polypeptides with apparent molecular weight of above 200, 100-120, 80 and 30-35 KDa that were readily removed upon wash with a high pH buffer which is known to remove clathrin and 0.5 M Tris-buffer which is known to remove assembly proteins (AP). Immunoblotting of GLUT4 and clathrin against specific antibodies showed that GLUT-4 and clathrin were co-solubilized up to 84.6% and 82.7% respectively by wash with a high pH buffer and 1% Tx-100. When the membrane was pre-washed with a high pH buffer and 0.5 M Tris solution, GLUT4 and clathrin were not solubilized further suggesting that GLUT4 molecules are in molecular association with clathrin, AP and/or other extrinsic membrane proteins in plasma membrane and the formation of clathrin-coated structures might be involved in insulin stimulated glucose transporter translocation mechanism.

• The Transactions of the Korea Information Processing Society
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• v.5 no.1
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• pp.236-248
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• 1998

Human observers perceive a contour where no line or edge is given, such as the border between gratings of thin lines offset by half a cycle. Illusory contours occur in a wide variety of circumstances in nature. A common factor in all such figures is the perception of a surface or contour occluding part of a background, i.e. illusory contours are always accompanied by illusory surfaces. Some examples about these kinds of figures are given by Kanizsa's triangle or square. In this paper, we proposed a neural network model for forming(extracting) the illusory surfaces, based on the mechanism of feature extraction found in a mammals' visual pathway. It will be shown that the model can extract the illusory surfaces from illusory contours successfully.

• International Journal of Control, Automation, and Systems
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• v.4 no.4
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• pp.438-447
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• 2006

Fuzzy controller's design depends mainly on the rule base and membership functions over the controller's input and output ranges. This paper presents two different approaches to deal with these design issues. A simple and efficient approach; namely, Fuzzy Subtractive Clustering is used to identify the rule base needed to realize Fuzzy PI and PD type controllers. This technique provides a mechanism to obtain the reduced rule set covering the whole input/output space as well as membership functions for each input variable. But it is found that some membership functions projected from different clusters have high degree of similarity. The number of membership functions of each input variable is then reduced using a similarity measure. In this paper, the fuzzy subtractive clustering approach is shown to reduce 49 rules to 8 rules and number of membership functions to 4 and 6 for input variables (error and change in error) maintaining almost the same level of performance. Simulation on a wide range of linear and nonlinear processes is carried out and results are compared with fuzzy PI and PD type controllers without clustering in terms of several performance measures such as peak overshoot, settling time, rise time, integral absolute error (IAE) and integral-of-time multiplied absolute error (ITAE) and in each case the proposed schemes shows an identical performance.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.17 no.2
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• pp.90-111
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• 2013

Objectives Migratory pathogenic factor(六淫) occupies an important position in the etiology of Korean Medicine. This paper shows how Dampness, one of Migratory pathogenic factor(六淫), is explained in Donguibogam(東醫寶鑑). And, based on this, we will figure out how to make a judgement of Dampness through diagnosis. Method 1. Collect parts of Dampness mentioned in Donguibogam(東醫寶鑑). 2. From the collection, extract contents about mechanism and symptom of Dampness, which is considered necessary for diagnosis. 3. Put all the extraction together, suggest the diagnosis element which can be criteria of judgement of Dampness through diagnosis. Result & Conclusions The occurrence of Dampness come from a wet climate and environment externally, and overeating of greasy food and digestive disorder internally. There are many different kinds of symptoms throughout the body cause of poor circulation. Dampness is classed as Cold-dampness, Damp-heat, and Dampness-phlegm depending on characteristic symptoms, and mainly shows musculoskeletal disease and digestive troubles. Typical symptoms are pitting edema, distention and fullness, moderate and thready pulse, volume of perspiration increase, loose feces, urine volume decrease, pain of joint and muscle, restriction of movement, etc.

• Corrosion Science and Technology
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• v.4 no.5
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• pp.211-216
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• 2005

Failure analysis of the latch ram ball and the C-ram ball with the trade name AFBMA Gr. 50 Colmonoy No. 6, has been performed to identify the root cause of the failure. The study required the extraction of the both failed and normal balls from the nuclear fuel exchanger. Microstructures of both balls were examined after polishing and etching. Breaking tests of both the ball revealed similarity in cleavage surfaces. Fracture surfaces of both failed ball and normal ball after breaking test were examined with SEM and EDX. Microstructure of the ball revealed an austenite phase with coarse Cr rich precipitate. Indented marks observed on the surface of the failed ball are believed to be produced by overloading. In the light of the afore mentioned observations and studies, the failure mechanism of the ball in nuclear fuel exchanger seem to be caused by impact or mechanical overloading on ball.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.3
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• pp.1189-1204
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• 2018

Detecting and capturing 3D human structures from the intensity-based image sequences is an inherently arguable problem, which attracted attention of several researchers especially in real-time activity recognition (Real-AR). These Real-AR systems have been significantly enhanced by using depth intensity sensors that gives maximum information, in spite of the fact that conventional Real-AR systems are using RGB video sensors. This study proposed a depth-based routine-logging Real-AR system to identify the daily human activity routines and to make these surroundings an intelligent living space. Our real-time routine-logging Real-AR system is categorized into two categories. The data collection with the use of a depth camera, feature extraction based on joint information and training/recognition of each activity. In-addition, the recognition mechanism locates, and pinpoints the learned activities and induces routine-logs. The evaluation applied on the depth datasets (self-annotated and MSRAction3D datasets) demonstrated that proposed system can achieve better recognition rates and robust as compare to state-of-the-art methods. Our Real-AR should be feasibly accessible and permanently used in behavior monitoring applications, humanoid-robot systems and e-medical therapy systems.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.1
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• pp.170-177
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• 1996

In this paper, we propose a neural network for extracting dashed lines from map images based on the feature extracting path be found in a cat and monkey's visual cortex. The proposed system converts a color image to a binary image and extracts the lines per orientation. Next, the system removes the lines longer than the dashed line and extracts the dashed lines by finding the consecutive short lines. The proposed system for extracting the dashed lines was tested using 1/25,000 scaled maps published by the National Geographic Institute of Korea. This paper shows that the proposed system can extract the dashed-lines from maps.

• Journal of Forest and Environmental Science
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• v.30 no.3
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• pp.267-276
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• 2014

Collection of lichen together with tree twigs of oak and other trees bearing abundant growth of lichens is a common practice among the villagers and the rivals residing near Oak forests in Kumaun Himalaya. Nainital forest division represents about Twenty nine percent vegetation of the Oak forest in Kumaun Himalaya. In Kumaun, the lichen trade share is decreasing at an alarming rate of 21.93% which requires immediate actions by the Government. Lichen contributed significantly to household earnings with off-farm activities and this sector was found second highest income creator after Agriculture. It is a source of cash income during the season of extraction, which increases economic access to food. It has been observed in the present study that the secondary collector and transporters together get maximum share (>50%) of income generated from lichen, thus economic exploitation of the poorly educated people by the traders was still prevalent in the area. To improve the socio-economic standard of the people of Kumaun, it may is necessary to increase and improve the lichens resources of the area. There is a strong need for scientific management, best harvesting practices and strict monitoring of resources. The present study was conducted to assess the present and future resource potential for the conservation and sustainable management of lichens, existing market mechanism, role of Lichens in economic upliftment and livelihood generation of rural communities in Kumaun Himalaya.

• The Journal of Korean Medicine
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• v.30 no.6
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• pp.17-26
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• 2009

Objectives: Little information is available regarding the effect of Lycii cortex radicis (LCR) on cell viability in glioma cells. This study was therefore undertaken to examine the effect of LCR on cell survival in U87MG human glioma cells. Methods: Cell viability and cell death were estimated by MTT assay and trypan blue exclusion assay, respectively. Reactive oxygen species (ROS) generation was measured using the fluorescence probe DCFH-DA. Activation of Akt and extracellular signal-regulated kinase (ERK) and activation of caspase-3 were estimated by Western blot analysis. Results: LCR resulted in apoptotic cell death in a dose- and time-dependent manner. LCR increased reactive oxygen species (ROS) generation and LCR-induced cell death was also prevented by antioxidants, suggesting that ROS generation played a critical role in LCR-induced cell death. Western blot analysis showed that LCR treatment caused down-regulation of Akt and ERK. The LCR-induced cell death was increased by the inhibitors of Akt and ERK. Activation of caspase-3 was stimulated by LCR and caspase inhibitors prevented the LCR-induced cell death. Conclusion: These findings suggest that LCR results in human glioma cell death through a mechanism involving ROS generation, down-regulation of Akt and ERK, and caspase activation.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.123-123
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• 1998

A number of uracil derivatives have been developed as anti-AIDS drugs having a mechanism of inhibiting cellular reverse transcriptase. A simple and rapid assay technique for recently synthesized KR-V analogues was developed using a high-performance liquid chromatography, and oral pharmacokinetics was examined for assessing their oral bioavailabilites. Plasma samples were analyzed by reversed-phase HPLC using an ODS column with an ultraviolet detection system. All the analogues were eluted within 12 min and the LOQ was 15-30 ng/$m\ell$. The extraction recoveries were higher than 85%, except KR-V1039, 1068 and 1720 having ester group. This chromatic method was well applied to the kinetic studies for KR-V analogues. Among 16 analogues tested in the present work, the 6 compounds including KR-V1123, 1122, 1784, 1783, 1736 and 1700 were found to be bioavailable for oral administration to rats.