• Journal of Periodontal and Implant Science
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• v.32 no.3
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• pp.665-683
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• 2002

A novel glucanhydrolase from a mutant of Lipomyces starkeyi KSM 22 has additional amylase activity besides mutanolytic activity and has been suggested as promising anti-plaque agent. It has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi KSM 22 glucanhydrolase are desirable for its application as a dental plaque control agent. In human experimental gingivitis　model and 6 month clinical trial, mouthrinsing with Lipomyces　starkeyi KSM 22 dextranase was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effect was negligible. This study was aimed to evaluate the cytotoxic effect of Lipomyces starkeyi KSM 22 glucanhydrolase on human gingival fibroblasts. Primary culture of human gingival fibroblasts at the 4th to 6th passages were used. Glucanhydrolase solution was made from lyophilized glucanhydrolase powder from a mutant of Lipomyces stakeyi KSM 22 solved in PBS and added to DMEM medium to the final concentration of 0.5, 1, and 2 unit. Cells were exposed to glucanhydrolase solution or 0.1 % chlorhexidine and the cells cultured in DMEM with 10% FBS and 1% antibiotics as control. After exposure, the morphological change, cell attachment, and cell activity by MTT assay were evaluated in 0.5, 1.5, 3, 6, 24 hours after treatment. The cell proliferation and cell activity was also evaluated at 2 and 7 days after 1 minute exposure, twice a day. The cell morphology was similar between the Lipomyces smkeyi KSM 22 glucanhydrolase groups and control group during the incubation periods, while most fibroblasts remained as round cell regardless of incubation time in the chlorhexidine group. The numbers of the attached cells in the glucanhydrolase groups were comparable to that of control and significantly higher than the chlorhexidine group. The numbers of the proliferated cells in the glucanhydrolase groups at 7 days of incubation were comparable to the control group and higher than the chlorhexidine group. The cell activity in glucanhydrolase groups paralleled with the increased cell number by attachment and proliferation. According to these results, Lipomyces starkeyj KSM 22 glucanhydrolase has little harmful effect on attachment and proliferation of human gingival fibroblasts, in contrast to 0.1% chlorhexidine which was cytotoxic to human gingival fibroblasts. Therefore this glucanhydrolase preparation is considered as a safe and promising agent for new mouthwash formula in the near future.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.1
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• pp.134-142
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• 2010

Although BinSo-San(BSS), a mixed herbal formula consisted of 11 types of medicinal herbs and have been used as anti-inflammatory agent, In the present study, the acute toxicity (single oral dose toxicity) of lyophilized BSS aqueous extracts was monitored in male and female mice after oral administration according to Korea Food and Drug Administration (KFDA) Guidelines (2005-60, 2005). In order to observe the 50% lethal dose ($LD_{50}$), approximate lethal dosage (ALD), maximum tolerance dosage (MTD) and target organs, test articles were once orally administered to female and male ICR mice at dose levels of 2000, 1000, 500, 250 and 0 (control) mg/kg (body wt.) according to the recommendation of KFDA Guidelines (2005-60, 2005). The mortality and changes on body weight, clinical signs and gross observation were monitored during 14 days after dosing according to KFDA Guidelines (2005-60, 2005) with organ weight and histopathology of 12 types of principle organs. We could not find any mortality, clinical signs and changes in the body weights except for dose-independent increases of body weight and gains restricted in 1000 mg/kg of BSS extracts-dosing female group. Hypertrophic changes of lymphoid organs.thymus, spleen and popliteal lymph nodes were detectedat postmortem observation with BSS extracts dose-dependent increases of lymphoid organ weights, and hyperplasia of lymphoid cells in these all three lymphoid organs at histopathological observations. These changes are considered as results of pharmacological effects of BSS extracts or their components, immunomodulating effects, not toxicological signs. In addition, some sporadic accidental findings such as congestion spots, cyst formation in kidney, atrophy of thymus and spleen with depletion of lymphoid cells, and edematous changes of uterus with desquamation of uterus mucosa as estrus cycles were detected throughout the whole experimental groups including both male and female vehicle controls. The significant (p<0.01) increases of absolute weights of kidney and pancreas detected in BSS extracts 1000 mg/kg-treated female group are considered as secondary changes from increases of body weights. The results obtained in this study suggest that the BSS extract is non-toxic in mice and is therefore likely to be safe for clinical use. The LD50 and ALD of BSS aqueous extracts in both female and male mice were considered as over 2000 mg/kg because no mortalities were detected upto 2000 mg/kg that was the highest dose recommended by KFDA and OECD. In addition, the MTD of BSS extracts was also considered as over 2000 mg/kg because no BSS extracts-treatment related toxicological signs were detected at histopathological observation except for BSS or their component-related pharmacological effects, the immunomodulating effects detected in the present study.

• Journal of Radiation Protection and Research
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• v.38 no.1
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• pp.22-28
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• 2013

The skyshine effect is an essential and important phenomenon in the shielding design of the high energy accelerator. In this study, a new estimation method of neutron skyshine was proposed and was verified by comparison with existing methods. The effective dose of secondary neutrons and photons at the locations that was far away from high-energy electron accelerator was calculated using FLUKA and PHITS Monte Carlo code. The transport paths of secondary radiations to reach a long distance were classified as skyshine, direct, groundshine and multiple-shine. The contribution of each classified component to the total effective dose was evaluated. The neutrons produced from the thick copper target irradiated by 10 GeV electron beam was applied as a source term of this transport. In order to evaluate a groundshine effect, the composition of soil on the PAL-XFEL site was considered. At a relatively short distance less than 50 m from the accelerator tunnel, the direct and groundshine components mostly contributed to the total effective dose. The skyshine component was important at a long distance. The evaluated dose of neutron skyshine agreed better with the results using Rindi's formula, which was based on the experimental results at high energy electron accelerator. That also agreed with the estimated dose using the simple evaluation code, SHINE3, within about 20%. The total effective dose, including all components, was 10 times larger than the estimated doses using other methods for this comparison. The influence of multiple-shine path in this evaluation of the estimation method was investigated to be bigger than one of pure skyshine path.

• Culinary science and hospitality research
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• v.22 no.5
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• pp.117-133
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• 2016

This study was to investigate the quality characteristics of sourdough bread made with 4 kinds of kamut sour starter. Kamut sour starters were made according to dough yield of sour starter and yeast adding on sour starter. We exmanined pH level, fermentation rate, specific volume, CrumbScan, moisture content, color value, texture analyzer, shelf-life test and sensory evaluation for observing quality characteristics of sourdough bread. From the results of pH measurement, kamut sourdough breads had lower pH value in dough and crumb pH than CON. In the experiment of dough's fermentation rate, CON had the rapidest fermentation rate among the samples initially, but the experimental groups also expanded as much as CON grew in spite of its slow fermentation. Specific volume and CrumbScan results suggested that CON had the largest volume. In addition, CM and TM had smaller volume than Pool and Biga. The sample that had the highest moisture content was Pool because its sour starter had the most amount of water. In color value test, CON had lighter crumb and crust color than other specimens. As a result of the TPA and shelf-life tests, CON had the softest hardness after 24 hours. However, Pool had similar hardness with CON after 72 hours. The result of the acceptance test of sourdough breads indicated that people prefered Pool and Biga to TM and CM. Difference test showed similar results with prior experiments. As the final outcome, Poolish was chosen as the optimal formula for kamut sour starter.

• Journal of the Society of Naval Architects of Korea
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• v.33 no.4
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• pp.1-14
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• 1996

Especially in the case of a full form ship, the stability on course can be considered to become severest among 4 items of criteria in Interim Standards for Ship Maneuverability adopted by IMO in 1993. The purpose of this study is to find some ideas for the improvement of stability on course through changes in rudder area with reference to span distance. In this paper, we established the formula on the relation between the experimental constants relevant to rudder normal force and hydrodynamic derivatives of hull-propeller-rudder system. We carried out various kinds of captive model test relevant to rudder normal force etc., and evaluated hydrodynamic derivatives of hull-propeller-rudder system, and analyzed the stability on course with the parameter of changes in rudder area. Furthermore, we also discussed effects of changes in rudder area on maneuvering performance including stability on course, based on computer simulation. As a result, it is clarified that there is a possibility that stability on course may become bad through an increase of rudder area. The reason for the bad stability on course is that the void space between the upper edge of rudder and the lower part of stern overhang decreases. This space change exerts a great influence on straightening coefficient of incoming flow to rudder in maneuvering motion, which has close relation to stability on course.

• Geophysics and Geophysical Exploration
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• v.9 no.2
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• pp.155-162
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• 2006

An abnormal seepage flow, which is mainly caused by the piping, is one of the major reasons for embankment dam failure. A leakage detection is therefore a vital part of an embankment dam's monitoring. Resistivity method, which is an efficient tool to detect leakage zones, has been used all over the world for an embankment dam's monitoring. Although the resistivity method gives us very useful information about the leakage problem, there is no more quantitative interpretation than the low resistivity zones in the 2-dimensional resistivity section are regraded simply as the anomalous seepage zones. Recently, resistivity monitoring technique is applied for the detection of leakage zones. However, its interpretation still remains in the stage of presenting the resistivity ratio itself. An increased seepage flow increases a porosity and an increasing porosity decreases the dam's stability. Therefore, the porosity is one of the major factors for an embankment dam's stability. Based on Archie's experimental formula, we try to evaluate a porosity distribution from the resistivity data which is obtained on the dam's crest. We also attempt to represent a procedure to evaluate a safety index of the embankment dam from the resistivity monitoring data.

• Clinical and Experimental Pediatrics
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• v.61 no.5
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• pp.135-149
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• 2018

Growth charts are curves or tables that facilitate the visualization of anthropometric parameters, and are widely used as an important indicator when evaluating the growth status of children and adolescents. The latest version of the Korean National Growth Charts released in 2007 has raised concerns regarding the inclusion of data from both breastfed and formula-fed infants, higher body mass index (BMI) values in boys, and smaller 3rd percentile values in height-for-age charts. Thus, new growth charts have been developed to improve the previous version. The 2006 World Health Organization Child Growth Standards, regarded as the standard for breastfed infants and children, were introduced for children aged 0-35 months. For children and adolescents aged 3-18 years, these new growth charts include height-for-age, weight-for-age, BMI-for-age, weight-for-height, and head circumference-for-age charts, and were developed using data obtained in 1997 and 2005. Data sets and exclusion criteria were applied differently for the development of the different growth charts. BMI-for-age charts were adjusted to decrease the 95th percentile values of BMI. Criteria for obesity were simplified and defined as a BMI of ${\geq}95th$ percentile for age and sex. The 3rd percentile values for height-for-age charts were also increased. Additional percentile lines (1st and 99th) and growth charts with standard deviation lines were introduced. 2017 Korean National Growth Charts are recommended for the evaluation of body size and growth of Korean children and adolescents for use in clinics and the public health sector in Korea.

• Journal of The Korean Association of Information Education
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• v.17 no.4
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• pp.433-447
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• 2013

The purpose of this study is to find out the factors of the students' achievement on the computer training courses which are based on computer practice. In order to improve the academic achievement of the students, it is necessary to analyze the factors affecting academic achievement and apply the results of the analysis to education. In particular, it is necessary to study for finding out factors of the academic achievement in practical computer training courses, because these courses are different from other courses focusing on the theory. In this study, in order to find out the factors, the logistic regression analysis and the decision tree analysis which is the field of data mining were peformed. For the experimental data, the test results of the MOS certification of the S university in seoul were used. Through logistic regression analysis it is found that the factors of the professors, class size, lecture time, group(lecture period) are important in order. Through decision tree analysis of data mining, it is found that there are some additional factors ; entrance year, whether the course is retaken, and the classroom environment. and these various factors effect the academic achievement compositively as identified through the model tree. The tree model was presented as a result of the analysis, and the importance of the factors is expressed numerically from multiple tree models by using the proposed mathematical formula.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.4
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• pp.651-658
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• 2001

A novel multi-section power divider configuration is proposed to obtain wide-band frequency performance up to microwave frequency region. Design procedures for the proposed microwave broadband power divider are composed of a planar multi-section three-ports hybrid and a waveguide transformer design procedures. The multi-section power divider is based on design theory of the optimum quarter-wave transformer. Furthermore, in order to obtain the broadband isolation performance between the two adjacent output ports, the odd mode equivalent circuit should be matched by using the lossy element such as resistor. The derived design formula for calculating these odd mode matching elements is based on the singly terminated filter design theory. The waveguide transformer section is designed to suppress the propagation of the higher order modes such as waveguide modes due to employing the metallic electric wall. Thus, each section of the designed waveguide transformer should be operated with evanescent mode over the whole design frequency band of the proposed microwave broadband power divider. This paper presents several simulations and experimental results of multi-section power divider to show validity of the proposed microwave broadband power divider configuration. Simulation and experiment show excellent performance of multi section power divider.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.22 no.1
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• pp.24-32
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• 2008

The ultimate purpose of the study is to develop a discomfort glare forecasting formula that can be practically used in Korea in order to effectively forecast discomfort glare considering the optical characteristics of the Koreans. The study was to examine the relations between discomfort glare and the variables such as luminance, background luminance, solid angle, luminous area and louver. To this end, experiments were conducted in a mock-up office that emulates the lighting environment of an ordinary office. The study was conducted by four steps as follow. First, previous studies on discomfort glare rating to define and rate discomfort glare were analyzed and modified to be applied to the experiments of this study. Second, experiment variables, variable scope, evaluation objects and evaluation points were determined after review on existing discomfort glare evaluation experimental formulas. Third, experiments were conducted in a mock-up office to be able to control variables. Finally, sensitivity of experiment variables were analyzed through examination of the relation between discomfort glare and the variables such as luminance, solid angle, louver, luminous area and subject's position. The result showed that the most influential variables on discomfort glare of an artificial light source is luminance and louver, luminous area and subject's position(solid angle) followed.