• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.5
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• pp.29-41
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• 2022

Alien species cause problems in various ecosystems, reduce biodiversity, and destroy ecosystems. Due to these problems, the problem of a management plan is increasing, and it is difficult to accurately identify each individual and calculate the number of individuals, especially when researching alien turtle species such as GPS and PIT based on capture. this study intends to conduct an individual recognition study using a UAV. Recently, UAVs can take various sensor-based photos and easily obtain high-definition image data at low altitudes. Therefore, based on previous studies, this study investigated five variables to be considered in UAV flights and produced a test paper using them. OCR was used to monitor the displayed turtles using the manufactured test paper, and this confirmed the recognition rate. As a result, the use of yellow numbers showed the highest recognition rate. In addition, the minimum threat distance was confirmed to be 3 to 6m, and turtles with a shell size of 6 to 8cm were also identified during the flight. Therefore, we tried to propose an object recognition methodology for turtle display text using OCR, and it is expected to be used as a new turtle monitoring technique.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.3
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• pp.29-45
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• 2022

For biodiversity conservation, the importance of beta-diversity which is changes in the composition of species according to environmental changes has become emphasized. However, given the systematic investigation of species distribution and the accumulation of large amounts of data in the Republic of Korea(ROK), research on the spatialization of beta-diversity using them is insufficient. Accordingly, this research investigated the applicability of the Generalized Dissimilarity Modeling(GDM) to ROK, which can predict and map the similarity of compositional turnover (beta-diversity) based on environmental variables. A brief overview of the statistical description on using GDM was presented, and a model was fitted using the flora distribution data(410,621points) from the National Ecosystem Survey and various environmental spatial data including climate, soil, topography, and land cover. Procedures and appropriated spatial units required to improve the explanatory power of the model were presented. As a result, it was found that geographical distance, temperature annual range, summer temperature, winter precipitation, and soil factors affect the dissimilarity of the vegetation community composition. In addition, as a result of predicting the similarity of vegetation composition across the nation, and classifying them into 20 and 100 zones, the similarity was high mainly in the central inland area, and tends to decrease toward the mountainous areas, southern coastal regions, and island including Jeju island, which means the composition of the vegetation community is unique and beta diversity is high. In addition, it was identified that the number of common species between zones decreased as the geographic distance between zones increased. It classified the spatial distribution of plant community composition in a quantitative and objective way, but additional research and verification are needed for practical application. It is expected that research on community-level biodiversity modeling in the ROK will be conducted more actively based on this study.

• Korean Journal of Ichthyology
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• v.32 no.2
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• pp.39-48
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• 2020

The slender shinner (Pseudopungtungia tenuicorpa), a tiny freshwater fish of about 8 to 10 cm belonging to Cyprinidae, is an endangered species found only in the Han and Imjin Rivers on the Korean Peninsula. During the breeding season, this species spawns in nests of Coreoperca herzi, a predator of this species, or small crevices on rocks. This unique reproductive ecology can make this species more vulnerable to anthropogenic perturbance that can further limit the places to spawn. Here, mtDNA and microsatellite loci were analyzed to identify the genetic diversity and structure of slender shinners and further to provide the basic data necessary for the conservation planning of this species. A total of 28 polymorphic microsatellite markers were developed using Illumina paired-end sequencing, and 67 slender shinners collected from three localities in the Han River were genotyped using these loci. This species showed a remarkably high level of genetic diversity with mean expected heterozygosity of 0.914 and mean allele number per locus of 27.9, and no signature of drastic demographic decline was detected. As a result of our microsatellite analysis, the genetic structure between the two stems of the Han River, North Han and South Han, was prominent. Such a genetic structure was also evident in the sequence analysis of 14 haplotypes obtained from mtDNA control region. Although slender shinners are only found in very limited areas around the world, the genetic structure indicates that there is a block of gene flow among the populations, which should be reviewed in the future if management and restoration of this species is needed.

• Korean Journal of Environment and Ecology
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• v.36 no.4
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• pp.368-380
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• 2022

Biomonitoring of an invasive alien crayfish species, Procambarus clarkii (Girard, 1852), was performed from February to October 2021, along with environmental DNA analysis, at five locations including Wanju-gun, Hampyeong-gun, Naju-si, Gurye-gun, and Cheongju-si. For the investigation, an umbrella-shaped trap for adults and a hand net for young crayfish were used, and 8 to 10L of freshwater was collected for eDNA analysis. The current status and past distributional records of crayfish in Korea were analyzed along with benthic macroinvertebrates at each survey site. As a result of the investigation, a total of 122 individuals were identified, and Hampyeong-gun recorded the largest number of populations with 59 individuals (48.36%) and the highest environmental DNA (eDNA). The frequency of appearance of P. clarkii was highest in May. The ratio of females to males was 21:5, and the body size was 72.2±21.1mm for female, 80.5±15.6mm for male, and 25.3±9.8mm for young crayfish. P. clarkii introduced into Korea is mainly spreading in the southwest region and it has not been observed in Seoul where there had been a record of appearance in the past. No external symbiosis (Branchiobdellida) of P. clarkii has been identified, and P. clarkii that has appeared in Korea was presumed to be imported from Japan. There are more than eight kinds of exotic crayfish distributed in Korea, and among them, the marbled crayfish (P. virginalis) was identified as a harmful species to the ecosystem of Korea by the Ministry of Environment in 2021. The identified species of benthic macroinvertebrates inhabiting the survey area where P. clarkii has appeared were 69 belonging to 39 families, 15 orders, five classes, and three phyla. Among them, Odonata were the most abundant (16 spp.; 24.62%), followed by Coleoptera (11 spp.; 16.92%) and Hemiptera (11 spp.; 16.92%). In the survey area, one Korean endemic species (Rhoenanthus coreanus), one species (Helophorus auriculatus) classified as Near Threatened (NT) on the Korean Red List, and six species of Crustaceans appeared. In the functional feeding group, the predators appeared predominantly while in habitat oriented group, the climbers appeared to be abundant. It was confirmed that P. clarkii in the survey area prefers an area rich in aquatic vegetation with waterside vegetation, and has high resistance to turbid water quality. The omnivore invader P. clarkii is expected to maintain a competitive relationship with carnivorous benthic macroinvertebrates that are predominant in the same species, and is expected to continue to generate ecosystem disturbance along the food chains.

• Korean Journal of Environment and Ecology
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• v.33 no.2
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• pp.168-177
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• 2019

This study was conducted to examine the impact of various environmental factors on the feeding behavior of black-faced spoonbills Platalea minor known to use rice fields during the breeding season. We surveyed the rice fields three times a month from April to June 2017 and from April to June 2018. We counted the number of black-faced spoonbills and measured the environmental factors such as the water depth, paddy type by cultivation stage, and biomass of potential prey that are expected to affect black-faced spoonbills. We also filmed the feeding behavior of black-faced spoonbills to examine differences in feeding behavior by environmental factors. The survey showed that the number of black-faced spoonbills reached its peak from late April to late May which was the breeding period. Among a total of 308 black-faced spoonbills observed, 86.4% (n=266) were observed feeding on rice paddies, 90.6% (n=279) were adults, and none was observed on dry paddies. The biomass of rice paddy increased significantly since May when fish started hatching, and there was a significant difference in the amount of biomass between the rice paddies where black-faced spoonbills were observed and other rice paddies. The analysis of the feeding behavior of black-faced spoonbills showed that the feeding efficiency and feeding success rate of black-faced spoonbills increased significantly when the amount of biomass soared and that the number of steps of black-faced spoonbills per minute increased greatly after rice planting was completed. The number of bill sweeping was not related to the feeding efficiency, feeding success rate, and paddy type by cultivation stage.

• Journal of Forest and Environmental Science
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• v.34 no.1
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• pp.82-86
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• 2018

Loblolly pine (Pinus taeda L.) is widely distributed in the southern part of the United States and it has been used as a major economic species in the region due to its excellent growth and stem straightness. The tree also grows only in the southern part of Korea because it is susceptible to cold. Recently climate changes have had widespread impacts on forest trees. Thus, the use of good quality seeds is prerequisite for assessing assisted migration adaptation trial. In this study, we conducted to investigate its cone and seed characteristics of each family, which is expected to improve seeds productivity for planting valuable timber trees. A total of 14 families were selected from the experimental forest in Boseong, Jeonnam province in 1981. The seed production capacity was estimated to range from 87.2 to 129.4 among families and the average was 111.3. The number of aborted ovules was investigated in the range of 11.4 to 29.5 for the first test and 7.4 to 22.2 for the second test. The average number of empty and filled seeds was 1.4 and 79.2 per cone, respectively. Based on the results, we can conclude that there is a strong correlation between the number of fertile scale and the seed production ability.

• Restorative Dentistry and Endodontics
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• v.24 no.1
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• pp.40-48
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• 1999

This study was designed to examine the specificities of the designed primers for F. nucleatum and F. necrophorum and to compare the PCR results using clinical samples with those of the anaerobic culture method. F. nucleatum and F. necrophorum spp. are frequently isolated in infected root canals, and they are related to periapical diseases. F. nucleatum(VPI 10197) and F. necrophorum(ATCC 25286) were used as references for PCR reaction, and thirty five teeth with one canal and periapical lesion were used. The samples were cultured anaerobically and identified using Rapid ID 32A(BioMerieux Vitek, Inc., France) as biochemical battery. In the GenBank database, species-specific PCR primers(nuc1/nuc2 primers for F. nucleatum and nec1/nec2 primers for F. necrophorum) were designed from the 16S ribosomal DNA(rDNA) sequences of F. nucleatum(accession number M58683) and F. necrophorum(accession number AF044948). PCR procedures of F. nucleatum(VPI 10197) and F. necrophorum (ATCC 25286) were simulated on a computer software. Amplify(v.1.2${\beta}$ for Macintosh). 820 bps and 817 bps of nucleotides were expected, respectively. Using extracted DNAs with QiaAmp tissue kit(Qiagen co., Germany), PCR was done. The results were as follows : 1. The nuc1/nuc2 primers produced an amplicon of 820 bps and the nec1/nec2 primers produced an amplicon of 817 bps. 2. The nuc1/nuc2 primers and the nec1/nec2 primers were specific and did not react with species other than the designated ones(i.e. nuc1/nuc2 primers did not produce amplicons for F. necrophorum, and vice versa.). And the PCR products of Porphyromonas endodontalis(ATCC 35406), Porphyromonas gingivalis(ATCC 33277), Prevotella intermedia(ATCC 25611), and Prevotella nigrescens(ATCC 33563), frequently isolated in infected root canals and periapical lesions, were not amplified by the primers specific for Fusobacterium nucleatum and Fusobacterium necrophorum. 3. This method utilizing PCR could detect F. nucleatum and F. necrophorum in clinical samples, while anaerobic culture method could detect neither.

• Korean Journal of Plant Resources
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• v.18 no.3
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• pp.479-489
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• 2005

The floras of Jisimdo, Naedo and Yundoldo around Koje Island were investigated 3 times from January 2004 to November 2004. The vascular plants recorded were 165 taxa, 1 forms, 15 varieties, 149 species, 140 genera, 72 families, The rare and endangered plants were recorded as 1 taxa (Quercus gilva Blume), Korean endemic plants were recorded as 2 taxa (1 variety, 1 species, 2 genera, 2 families). The naturalized plants were 10 taxa, 10 species, 10 genera, 6 families, $3.52\%\;and\;6.1\%$ among the total naturalized plants and Urbanization Index in Korea. Anglers and visiter's number is expected to increase fast because nature of Jisimdo, Naedo and Yundoldo, in which kind and number of naturalized plants are forecasted to be increased more.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.1
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• pp.69-80
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• 2021

The new town in Korea, developed as a large-scale housing plan, has created urban ecological corridors to provide habitat and movement routes to wildlife and to promote natural ecological flow. This study aimed to investigate the use of wildlife in 10 ecological corridors in Gwanggyo New Town through camera trap technology and confirm effectiveness by identifying environmental factors affecting the use of wildlife's urban ecological corridors. Our researchers installed 20 unmanned sensor cameras at each the entrance and exit of the ecological corridors, and monitored urban wildlife for 10 weeks. According to the monioring results, the main species in Gwanggyo New Town were identified not only raccons, cats, water deer, korean hare and avain but also magpies, dove, eurasian tree sparrow, ring-necked pheasant, and eurasian jay. The number of uses ecological corridors of urban residents was 801(13.49%), as high as that of urban wildlife (1,140, 19.20%), which was judged to have disturbed the use of ecological corridors by wildlife. However, most dominant species of urban wildlife are nocturnal so that, it was judged that they share home range with urban residents at a time interval. In addition, according to the correlation analysis results between the mammal using rate of the urban ecological corridors and environmental factors(ecological corridor-specific length, ecological corridor-specific width, cover degree, shielding degree, connected green area, separation of movement routes, and presence of streetlights), environmental factors were not statistically significant. However, the more the area of green space connected to ecological corridors, the more increasing the mammal using rate of ecological corridor(r=0.71, p<0.05). Therefore, the area of green space connected to the ecological corridors that is associated with rate of wildlife using corridors should be considered as a priority when developing an urban ecological corridors. In the future, this study will extend the observation period of the ecological corridors and continuously accumulate data by adding the number of observation cameras. Furthermore, it is expected that the results of this study can be used as basic data for the standards for urban ecological corridors installation.

• Korean Journal of Ecology and Environment
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• v.55 no.4
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• pp.285-293
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• 2022

Sampling gears for collecting fish are diverse, and the community of fish varies according to the selection and characteristics of the sampling gears. The present study compared the characteristics of fish communities in Yedang reservoir using four sampling gears (kick net, cast net, gill net, and fyke net). The kick net and cast net were inefficient in collecting the number of individuals. However, they increased the species diversity of fish inhabiting the waterfront. Although not many individuals were collected, the gill net mainly collected large fish. The largest number of individuals was collected in the fyke net, and the dominance was high due to the high species selectivity. Through Self-Organizing Map (SOM) analysis, large fish were collected in the gill net, whereas small fish were collected in the fyke net. The characteristics and efficiency of the fish differed depending on the sampling gears. It is expected that researchers will need to use it appropriately according to the characteristics of the sampling gears when investigating the fish community.